Abstract:
:The relationship between sugar availability and RTX (repeats in toxin) cytotoxin (leukotoxin) production in the periodontopathic bacterium, Actinobacillus actinomycetemcomitans, was investigated using a chemostat. A actinomycetemcomitans 301-b produced significant amounts of leukotoxin in anaerobic fructose-limited chemostat cultures at a dilution rate of 0.15 h-1 and at pH 7.0. When the growth limitation was relieved by pulsing the cultures with 50 or 150 mM fructose (final concentrations), leukotoxin production immediately stopped and the amount of cellular leukotoxin decreased until the culture was returned to fructose-limited conditions. Leukotoxin synthesis was also repressed in the chemostat cultures by pulsing with glucose but not with the non-fermentable sugar analog, alpha-methyl-D-glucoside. Leukotoxin production was also repressed by fructose in chemostat cultures of ATCC 33384, which is generally recognized as a non-leukotoxin-producing or minimally leukotoxic strain.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Mizoguchi K,Ohta H,Miyagi A,Kurihara H,Takashiba S,Kato K,Murayama Y,Fukui Kdoi
10.1111/j.1574-6968.1997.tb10187.xsubject
Has Abstractpub_date
1997-01-01 00:00:00pages
161-6issue
1eissn
0378-1097issn
1574-6968pii
S0378109796004739journal_volume
146pub_type
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