spyder, a new method for in silico design and assessment of 16S rRNA gene primers for molecular microbial ecology.

abstract：:Phlebia tremellosa decolourised eight synthetic textile dyes (200 mg l(-1)) by greater than 96% within 14 days under stationary incubation conditions. High performance liquid chromatography analysis of culture supernatants indicated that Remazol Black B was degraded by the fungus, however, complete mineralisation did ...

journal_title：FEMS microbiology letters

authors： Kirby N,Marchant R,McMullan G

更新日期：2000-07-01 00:00:00

abstract：:The Giardia genome project database provides an online resource for Giardia lamblia (WB strain, clone C6) genome sequence information. The database includes edited single-pass reads, the results of BLASTX searches, and details of progress towards sequencing the entire 12 million-bp Giardia genome. Pre-sorted BLASTX re...

journal_title：FEMS microbiology letters

authors： McArthur AG,Morrison HG,Nixon JE,Passamaneck NQ,Kim U,Hinkle G,Crocker MK,Holder ME,Farr R,Reich CI,Olsen GE,Aley SB,Adam RD,Gillin FD,Sogin ML

更新日期：2000-08-15 00:00:00

abstract：:Contrary to its effect on rich medium, D-cycloserine showed no bactericidal effect on Zymomonas mobilis cells cultured on mineral medium. Addition of a mixture of glycine and glutamic acid to the mineral medium restored its bactericidal action. However, mutant enrichments run in these conditions were biased, with most...

journal_title：FEMS microbiology letters

authors： Pencreac'h G,Aït-Abdelkader N,Joset F,Baratti JC

更新日期：1996-09-01 00:00:00

abstract：:The RS1 element associated with Vibrio cholerae CTX phi prophage was cloned from an E1 Tor biotype Vibrio cholerae strain. We used the recA- vaccine strain Peru-15, that lacks the target for RS-mediated site-specific integration, to show that RS1 promotes autonomous replication of a suicide vector. A linker insertion ...

journal_title：FEMS microbiology letters

authors： Campos J,Fando R,Silva A,Rodriguez BL,Benitez JA

更新日期：1998-07-01 00:00:00

abstract：:TraJ is an activator of the transfer (tra) operon in the F plasmid that counteracts H-NS silencing at the main transfer promoter (P(Y)). TraJ contains 226 aa (26 670 kDa), not 229 aa as reported previously, and forms homodimers. TraJ binds DNA containing P(Y)in vivo as demonstrated using a chromatin-immunoprecipitatio...

journal_title：FEMS microbiology letters

authors： Rodriguez-Maillard JM,Arutyunov D,Frost LS

更新日期：2010-09-01 00:00:00

abstract：:The high-pathogenicity island (Yen HPI) of Yersinia enterocolitica biogroup (BG) 1B strains is associated with mouse virulence. Three repeated sequences are clustered on the A+T-rich part of the Yen HPI downstream of the fyuA yersiniabactin receptor gene in Y. enterocolitica O:8 strains WA-314 and 8081. In addition to...

journal_title：FEMS microbiology letters

authors： Rakin A,Schubert S,Guilvout I,Carniel E,Heesemann J

更新日期：2000-01-15 00:00:00

abstract：:The main determinant of the plant pathogen Erwinia chrysanthemi virulence is the production of extracellular enzymes, mainly pectate lyases. Adjacent to a pectate lyase encoding locus, we identified the gene rotA supposed to encode a folding catalyst. Overproduction of the protein and assay of activity using a synthet...

journal_title：FEMS microbiology letters

authors： Pissavin C,Hugouvieux-Cotte-Pattat N

更新日期：1997-12-01 00:00:00

abstract：:Many bacteria are capable of accumulating intracellular granules of polyhydroxyalkanoates (PHA). In this work, we developed confocal microscopy analysis of bacterial cells to study changes in the diameters of cells as well as PHA granules during growth and PHA accumulation in the bacterium Cupriavidus necator H16 (for...

journal_title：FEMS microbiology letters

authors： Mravec F,Obruca S,Krzyzanek V,Sedlacek P,Hrubanova K,Samek O,Kucera D,Benesova P,Nebesarova J

更新日期：2016-05-01 00:00:00

abstract：:The genes encoding transferrin-binding proteins (TBPs) 1 and 2 of Neisseria meningitidis and N. gonorrhoeae were used as model loci in a novel method of cloning (twin N-terminal polymerase chain reaction; TNT-PCR) involving amplification between the 5' ends of two genes. Primers were based on N-terminal amino-acid seq...

journal_title：FEMS microbiology letters

authors： Wilton J,Ala'Aldeen D,Palmer HM,Borriello SP

更新日期：1993-02-15 00:00:00

abstract：:We report on the specificity of a monoclonal antibody which reacts with autoclaved extracts of four species of enterococci but does not react to the same extent with similar extracts from two non-enterococcal group D streptococci. The monoclonal antibody also reacts specifically with purified lipoteichoic acid from St...

journal_title：FEMS microbiology letters

authors： Hammonds SJ,Easterbrook TJ,McIllmurray MB

更新日期：1991-07-15 00:00:00

abstract：:A bacterial isolate, tentatively identified as Pseudomonas sp. strain TR3, was found to utilize the diaryl ester phenylbenzoate as sole source of carbon and energy. This strain has the ability to productively degrade phenylbenzoate and some substituted derivatives by a catabolic sequence which was characterized bioche...

journal_title：FEMS microbiology letters

authors： Reich T,Schmidt S,Fortnagel P

更新日期：1999-07-15 00:00:00

abstract：:A murine monoclonal antibody, designated MA-2G9, directed against outer surface protein A (OspA) of the Lyme disease spirochete, Borrelia burgdorferi, has been produced. Antibody MA-2G9, IgG1 subclass, was purified by affinity chromatography on protein G Sepharose column and used for purification of OspA antigen from ...

journal_title：FEMS microbiology letters

authors： Abolhassani M,Pavia CS,Dunn JJ,Chiao JW

更新日期：1993-05-15 00:00:00

abstract：:The nucleotide sequence of the gene specifying the ethidium efflux system of Escherichia coli has been determined. The translated open reading frame has identified a membrane-bound polypeptide of 110 amino acids (11,960 Da) which shares 42% identity with a staphylococcal protein specifying resistance to ethidium. ...

journal_title：FEMS microbiology letters

authors： Purewal AS

更新日期：1991-08-01 00:00:00

abstract：:A metagenomic approach was taken to investigate the genetic basis for the ability of an anaerobic consortium to grow on either 4-chlorobenzoate or 4-bromobenzoate under denitrifying conditions. Degenerate PCR primers were designed for the family of 4-chlorobenzoyl-CoA dehalogenase genes. The primers were utilized to s...

journal_title：FEMS microbiology letters

authors： Chae JC,Song B,Zylstra GJ

更新日期：2008-04-01 00:00:00

abstract：:A high-level expression system for the Escherichia coli HB101 gamma-glutamyltranspeptidase (GGT) gene was constructed to obtain a sufficient amount of the enzyme for structural studies. About 300 mg of the enzyme was purified from 2 1 of culture broth. Both subunits were needed to reconstitute GGT activity. The E. col...

journal_title：FEMS microbiology letters

authors： Ishiye M,Niwa M

更新日期：1992-10-15 00:00:00

abstract：:Deferoxamine (DFO), an FDA-approved iron chelator used for treatment of iron poisoning, affects bacteria as iron availability is intimately connected with growth and several virulence determinants. However, little is known about the effect on oral pathogens. In this study, the effect of DFO on Porphyromonas gingivalis...

journal_title：FEMS microbiology letters

authors： Moon JH,Herr Y,Kim SW,Lee JY

更新日期：2011-10-01 00:00:00

abstract：:Escherichia coli heat-labile enterotoxin B subunit (EtxB) has been proposed as a potential protein carrier for the delivery of heterologous peptides to target cells, particularly for the oral delivery of epitopes to the mucosal immune system. In this study, two extensions to the C-terminus of EtxB were genetically eng...

journal_title：FEMS microbiology letters

authors： Marcello A,Loregian A,Palù G,Hirst TR

更新日期：1994-03-15 00:00:00

abstract：:Nitrogen is a key nutrient for land plants and phytoplankton in terrestrial and aquatic ecosystems. The model alga Chlamydomonas reinhardtii can grow efficiently on several inorganic nitrogen sources (e.g. ammonium, nitrate, nitrite) as well as many amino acids. In this study, we show that Chlamydomonas is unable to u...

journal_title：FEMS microbiology letters

authors： Calatrava V,Hom EFY,Llamas Á,Fernández E,Galván A

更新日期：2018-04-01 00:00:00

abstract：:A solvent-tolerant, slightly thermophilic bacterium was isolated at 45 degrees C in the presence of toluene vapor provided as the sole carbon source. Strain T27 was identified as Deinococcus geothermalis T27. It could tolerate high concentrations of solvent provided as a nonaqueous layer (5% and 20%, v/v) to a cell su...

journal_title：FEMS microbiology letters

authors： Kongpol A,Kato J,Vangnai AS

更新日期：2008-09-01 00:00:00

abstract：:The anr gene of Pseudomonas aeruginosa encodes a transcriptional regulator of anaerobic gene expression, homologous to the Fnr protein of Escherichia coli. We report here that Anr has a role in regulating the activity of the aerobic respiratory chain of P. aeruginosa. Strains with internal deletions in their anr gene ...

journal_title：FEMS microbiology letters

authors： Ray A,Williams HD

更新日期：1997-11-15 00:00:00

abstract：:We have developed a novel type of a positive screen for the discovery of antibacterial compounds that target the Escherichia coli replication initiator protein DnaA. DnaA is an essential replication protein, conserved in (almost) all bacteria--including all human pathogens--and no existing antibiotics target the main ...

journal_title：FEMS microbiology letters

authors： Fossum S,De Pascale G,Weigel C,Messer W,Donadio S,Skarstad K

更新日期：2008-04-01 00:00:00

abstract：:Mycobacterium sp. PYR-1 was previously isolated in our laboratory and was shown to be able to mineralize high molecular mass polycyclic aromatic hydrocarbons (PAHs) [Heitkamp and Cerniglia, (1988) Appl. Environ. Microbiol. 54, 1612-1614]. In this research, the 16S rRNA gene (rDNA) of this strain was amplified by polym...

journal_title：FEMS microbiology letters

authors： Wang RF,Cao WW,Cerniglia CE

更新日期：1995-07-15 00:00:00

abstract：:Cytoplasmic precursors of the peptidoglycan biosynthetic pathway were purified from vancomycin-treated, glycopeptide-sensitive and -resistant strains of Enterococcus faecium. Resistance was due to production of a modified precursor, UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-lactate, where lactate was identified on the basi...

journal_title：FEMS microbiology letters

authors： Messer J,Reynolds PE

更新日期：1992-07-01 00:00:00

abstract：:Hyphae of the human pathogenic fungus Candida albicans exhibit thigmotropic behaviour in vitro, in common with phytopathogenic and saprotrophic fungi. An examination of the literature on C. albicans hyphal penetration of epithelial and endothelial membranes does not support the premise that hyphal thigmotropism plays ...

journal_title：FEMS microbiology letters

authors： Davies JM,Stacey AJ,Gilligan CA

更新日期：1999-02-15 00:00:00

abstract：:A Phoma sp. was isolated and characterized as endophytic and as a pathogen of Larrea tridentata (creosote bush) growing in the desert region of southern Utah, USA. This fungus produces a unique mixture of volatile organic compounds (VOCs), including a series of sesquiterpenoids, some alcohols and several reduced napht...

journal_title：FEMS microbiology letters

authors： Strobel G,Singh SK,Riyaz-Ul-Hassan S,Mitchell AM,Geary B,Sears J

更新日期：2011-07-01 00:00:00

abstract：:Two actinomycete strains, CHR3 and CHR28, were isolated from metal-contaminated sediments from Baltimore Inner Harbor. The isolates were classified as Streptomyces spp. Agar diffusion assays showed both isolates to be resistant to mercuric chloride and phenylmercuric acetate. Hybridization experiments indicated that g...

journal_title：FEMS microbiology letters

authors： Ravel J,Amoroso MJ,Colwell RR,Hill RT

更新日期：1998-05-01 00:00:00

abstract：:The polyhydroxyalkanoate (PHA) synthase (PhaC(Da)) from Delftia acidovorans DS-17 (formerly Comamonas acidovorans) has a unique large insertion consisting of 40 amino acid residues in the alpha/beta hydrolase fold region. In order to examine whether this insertion is necessary for enzyme function, we generated a mutan...

journal_title：FEMS microbiology letters

authors： Tsuge T,Imazu S,Takase K,Taguchi S,Doi Y

更新日期：2004-02-09 00:00:00

abstract：:Bacteriophages infecting Salmonella spp. were isolated from sewage using soft agar overlays containing three Salmonella serovars and assessed with regard to their potential to control food-borne salmonellae. Two distinct phages, as defined by plaque morphology, structure and host range, were obtained from a single sam...

journal_title：FEMS microbiology letters

authors： Carey-Smith GV,Billington C,Cornelius AJ,Hudson JA,Heinemann JA

更新日期：2006-05-01 00:00:00

abstract：:The polymerase chain reaction was used to search for hepatitis B virus (HBV)-DNA sequences in the sera of HBsAg-negative Senegalese patients suffering from liver cirrhosis or liver cancer. Amplified HBV-DNA sequences were detected by hybridization with a digoxigenin-labelled HBV-DNA probe. HBV-DNA was detected in 17% ...

journal_title：FEMS microbiology letters

authors： Coursaget P,Le Cann P,Leboulleux D,Diop MT,Bao O,Coll AM

更新日期：1991-09-15 00:00:00

abstract：:Desulfovibrio fructosovorans possesses two periplasmic hydrogenases (a nickel-iron and an iron hydrogenase) and a cytoplasmic NADP-dependent hydrogenase. The hydAB genes encoding the periplasmic iron hydrogenase were replaced, in the wild-type strain as well as in single mutants depleted of one of the other two hydrog...

journal_title：FEMS microbiology letters

authors： Casalot L,Valette O,De Luca G,Dermoun Z,Rousset M,de Philip P

更新日期：2002-08-27 00:00:00