Cloning and partial sequence of transferrin-binding protein 2 of Neisseria meningitidis using a novel method: twin N-terminal PCR.

abstract：:The ectomycorrhizal fungus Paxillus involutus efficiently took up exogenously supplied [14C]alanine and rapidly converted it to pyruvate, citrate, succinate, fumarate and to CO2, thus providing direct evidence for the utilisation of alanine as a respiratory substrate. [14C]alanine was further actively metabolised to g...

journal_title：FEMS microbiology letters

authors： Chalot M,Brun A,Finlay RD,Söderström B

更新日期：1994-08-01 00:00:00

abstract：:We developed a DNA microarray for identification of Bacillus anthracis and other phylogenetic groupings within the "Bacillus cereus group". Nucleotide sequences of 16S-23S ribosomal DNA internal transcribed spacers containing genes for tRNA(Ile) from 52 B. anthracis strains were found to be identical to sequences from...

journal_title：FEMS microbiology letters

authors： Nübel U,Schmidt PM,Reiss E,Bier F,Beyer W,Naumann D

更新日期：2004-11-15 00:00:00

abstract：:High voltage electroporation has been investigated as a method for rapid recovery of plasmid and chromosomal DNA from the cyanobacteria Nostoc PCC 7121, Synechococcus PCC 7002, and Anabaena PCC 7120. Pulses of 18 kV/cm and higher applied to concentrated Nostoc cells carrying a shuttle plasmid (pRL25) resulted in copio...

journal_title：FEMS microbiology letters

authors： Moser D,Zarka D,Hedman C,Kallas T

更新日期：1995-05-15 00:00:00

abstract：:Correct identification of Campylobacter jejuni and Campylobacter coli isolates to the species or subspecies level is a cumbersome but nevertheless important task for a routine diagnostic laboratory. The widely used biochemical tests might be often misleading while more sophisticated phenotypic or genotypic methods are...

journal_title：FEMS microbiology letters

authors： Steinbrueckner B,Haerter G,Pelz K,Kist M

更新日期：1999-10-15 00:00:00

abstract：:The accumulation of 2-keto-3-deoxy-6-phosphogluconate, the key intermediate of the Entner-Doudoroff pathway, has long been thought to inhibit growth of bacteria, but careful measurements of 2-keto-3-deoxy-6-phosphogluconate accumulation by growing cells and the correlation of intracellular 2-keto-3-deoxy-6-phosphogluc...

journal_title：FEMS microbiology letters

authors： Fuhrman LK,Wanken A,Nickerson KW,Conway T

更新日期：1998-02-15 00:00:00

abstract：:Arbuscular mycorrhizal fungi (AMF) have been extensively studied in natural and agricultural ecosystems, but little is known about their diversity and community structure in highly petroleum-polluted soils. In this study, we described an unexpected diversity of AMF in a sedimentation basin of a former petrochemical pl...

journal_title：FEMS microbiology letters

authors： de la Providencia IE,Stefani FO,Labridy M,St-Arnaud M,Hijri M

更新日期：2015-06-01 00:00:00

abstract：:A cordycepin-resistant mutant strain of Saccharomyces cerevisiae (CD-R2) was found to be deficient in adenosine kinase. This mutant accumulated S-adenosylhomocysteine during growth in the presence of exogenous adenosine and it grew in a pseudohyphal manner in the presence of this nucleotide. ...

journal_title：FEMS microbiology letters

authors： Iwashima A,Ogata M,Nosaka K,Nishimura H,Hasegawa T

更新日期：1995-03-15 00:00:00

abstract：:Salmonella is a leading cause of waterborne diseases. Salmonella can survive for a long time in aquatic environments, and its persistence in the environment is of great concern to public health. Nonetheless, the presence and diversity of Salmonella in the aquatic environments in most areas remain relatively unknown. I...

journal_title：FEMS microbiology letters

authors： Huang KH,Hsu BM,Chou MY,Tsai HL,Kao PM,Wang HJ,Hsiao HY,Su MJ,Huang YL

更新日期：2014-03-01 00:00:00

abstract：:Yeast two-hybrid and Far Western analyses were used to detect interactions between Bacillus subtilis spores' nutrient germinant receptor proteins and proteins encoded by the spoVA operon, all of which are involved in spore germination and located in the spores' inner membrane. These analyses indicated that two subunit...

journal_title：FEMS microbiology letters

authors： Vepachedu VR,Setlow P

更新日期：2007-09-01 00:00:00

abstract：:Tn10 transposon mutagenesis of Escherichia coli producing K99 fimbriae was carried out to identify host factors involved in regulation of biosynthesis of fimbriae. Two chromosomal mutants were obtained that showed a strongly reduced cell surface expression of K99 fimbriae upon colony blotting and ELISA. Analysis by in...

journal_title：FEMS microbiology letters

authors： Pilipcinec E,Huisman TT,Willemsen PT,Appelmelk BJ,de Graaf FK,Oudega B

更新日期：1994-10-15 00:00:00

abstract：:The polyhydroxyalkanoic acid (PHA) synthase gene (phaCAc) of a species of Acinetobacter isolated from an activated sludge treatment plant was cloned by heterologous complementation in a poly-beta-hydroxybutyrate (PHB) negative mutant of Alcaligenes eutrophus. Nucleotide sequence analysis of phaCAc revealed an open rea...

journal_title：FEMS microbiology letters

authors： Schembri MA,Bayly RC,Davies JK

更新日期：1994-05-01 00:00:00

abstract：:Based on amino acid sequence similarities between the methylated elongation factor EF-Tu from Escherichia coli and the EF-Tu from Euglena gracilis chloroplast, we predicted that the latter could also be methylated in the presence of an appropriate methyltransferase. We found that, as reported for the eubacterial homol...

journal_title：FEMS microbiology letters

authors： Toledo H,Jerez CA

更新日期：1990-09-15 00:00:00

abstract：:Production of the gene transfer agent of Rhodobacter capsulatus, RcGTA, is dependent upon several cellular regulatory systems, including a putative phosphorelay involving the CtrA and CckA proteins. These proteins are also involved in flagellar motility in R. capsulatus. The interactions of proteins in this system are...

journal_title：FEMS microbiology letters

authors： Mercer RG,Quinlan M,Rose AR,Noll S,Beatty JT,Lang AS

更新日期：2012-06-01 00:00:00

abstract：:High resistance to class IIa bacteriocins in Listeria monocytogenes has been clearly linked to lack of expression of the mptACD operon, encoding the EIIt Man mannose PTS permease. Also, intermediate resistance has been associated with membrane phospholipid modifications in the spontaneous mutants L. monocytogenes B73-...

journal_title：FEMS microbiology letters

authors： Arous S,Dalet K,Héchard Y

更新日期：2004-09-01 00:00:00

abstract：:Tachyzoites of Toxoplasma gondii multiply within the parasitophorous vacuole (PV) until the lysis of the host cell. This study was undertaken to evaluate the effect of hydroxyurea (a specific drug that arrests cell division at G1/S phase) on the multiplication of T. gondii tachyzoites in infected Vero cells. Infected ...

journal_title：FEMS microbiology letters

authors： de Melo EJ,Mayerhoffer RO,de Souza W

更新日期：2000-04-01 00:00:00

abstract：:Outer membrane-associated proteins of 18 kDa were expressed by enteroaggregative Escherichia coli (EAggEC) belonging to serotypes O126:H27 and O44:H18, which hybridized with a probe derived from a plasmid necessary for enteroaggregative adhesion. The 18 kDa proteins expressed by strains of E. coli, belonging to these ...

journal_title：FEMS microbiology letters

authors： Chart H,Smith HR,Rowe B

更新日期：1995-10-01 00:00:00

abstract：:The Great Salt Plains is a 65-km(2) hypersaline habitat of geological origin located in north-central Oklahoma. Contamination of such ecosystems by petroleum compounds is expected from non-point sources and due to increased human activities. Little information exists about the ability of halophilic and halotolerant ba...

journal_title：FEMS microbiology letters

authors： Nicholson CA,Fathepure BZ

更新日期：2005-04-15 00:00:00

abstract：:Antibiotic therapies are known to disrupt gastrointestinal (GI) bacterial communities. HIV and pathogenic simian immunodeficiency virus (SIV) infections have also been associated with disrupted GI bacterial communities. We administered a combination antibiotic therapy to six SIV-infected rhesus macaques and collected ...

journal_title：FEMS microbiology letters

authors： Zevin AS,Hensley-McBain T,Miller C,Smith E,Langevin S,Klatt NR

更新日期：2017-12-15 00:00:00

abstract：:Thermophilic bacteria have recently attracted great attention because of their potential application in improving different biochemical processes such as anaerobic digestion of various substrates, wastewater treatment or hydrogen production. In this study we report on the design of a specific 16S rRNA-targeted oligonu...

journal_title：FEMS microbiology letters

authors： Gagliano MC,Braguglia CM,Rossetti S

更新日期：2014-09-01 00:00:00

abstract：:The polymerase chain reaction (PCR)-based procedures of randomly amplified polymorphic DNA (RAPD) and repetitive element (RE)-based PCR were used to amplify total DNA prepared from each of 62 clinical Serratia marcescens isolates. Three different random primers, designated 1060, 1254 and 1283, were used individually i...

journal_title：FEMS microbiology letters

authors： Patton TG,Katz S,Sobieski RJ,Crupper SS

更新日期：2001-01-01 00:00:00

abstract：:Researchers studying both cellular and viral disease agents in the laboratory have become infected since the early days of microbiology 150 years ago. However, in the early 21st century, new concerns about bioweapons being used to generate terror and also with a series of newly emerging or newly understood disease-cau...

journal_title：FEMS microbiology letters

authors： Silver S

更新日期：2015-01-01 00:00:00

abstract：:Proteus mirabilis strain MAG1, a clinical isolate that is resistant to broad-spectrum penicillins and co-amoxiclav, produces inhibitor-resistant TEM (IRT)-21, a novel mutant of TEM beta-lactamase. This enzyme has a pI of 5.2 and is derived from the bla(TEM-1a) gene ancestor. It contains two major amino acid substituti...

journal_title：FEMS microbiology letters

authors： Mammeri H,Gilly L,Laurans G,Vedel G,Eb F,Paul G

更新日期：2001-12-18 00:00:00

abstract：:Changes in composition of the principal low molecular mass thiols of Leishmania donovani were monitored during the transformation of promastigotes, first to stationary phase metacyclic forms and then to amastigotes. No consistent variation in the thiol composition of the parasite which could account for the known incr...

journal_title：FEMS microbiology letters

authors： Weldrick DP,Chodacka B,Vogt R,Steenkamp DJ

更新日期：1999-04-01 00:00:00

abstract：:The presence of insertion sequence IS1 in 70 multiple-antibiotic resistant clinical strains was determined. This 70-strain collection comprised 46 Escherichia coli, 18 Salmonella and 6 Shigella strains. The presence of IS1 was detected in the chromosome and plasmids of 73% and 63% of the strains, respectively, and 51%...

journal_title：FEMS microbiology letters

authors： Ramirez-Santos J,Alvarez G,Cisneros E,Gomez-Eichelmann MC

更新日期：1992-06-01 00:00:00

abstract：:In order to clarify the enteropathogenicity of Plesiomonas shigelloides, we investigated a cytotoxin produced by the P-1 strain isolated from patients suffering from diarrhea. The cytotoxicity of the culture filtrate of the strain reached a maximum in culture at 37 degrees C after 12 h shaken in BHI medium. The cytoto...

journal_title：FEMS microbiology letters

authors： Okawa Y,Ohtomo Y,Tsugawa H,Matsuda Y,Kobayashi H,Tsukamoto T

更新日期：2004-10-01 00:00:00

abstract：:Volvariella volvacea, strain V14, produces multiple forms of extracellular laccase when grown in submerged culture in a defined medium with glucose as sole carbon source, and on cotton waste 'compost' representative of the conditions used for industrial-scale mushroom cultivation. In liquid culture, enzyme synthesis i...

journal_title：FEMS microbiology letters

authors： Chen S,Ma D,Ge W,Buswell JA

更新日期：2003-01-21 00:00:00

abstract：:The identification of virulence factors in Rhodococcus equi has been severely hampered by the lack of a method for in vivo random insertion mutagenesis. This study reports the use of transposomes to generate random insertions of a gene conferring kanamycin resistance into the genome of R. equi ATCC 33701. Southern hyb...

journal_title：FEMS microbiology letters

authors： Mangan MW,Meijer WG

更新日期：2001-12-18 00:00:00

abstract：:The growth of Mycobacterium microti was inhibited within J774A.1 macrophage cells activated with either interferon-gamma or tumor necrosis factor-alpha. Activation with interferon-gamma or tumor necrosis factor-alpha alone did not stimulate the production of nitrite in J774A.1 cells. Interferon-gamma but not tumor nec...

journal_title：FEMS microbiology letters

authors： Gupta R,Dogra N,Raje M,Majumder S

更新日期：1996-07-01 00:00:00

abstract：:Using pulsed-field gel electrophoresis (PFGE), nine profile types were identified in XbaI digests of a panel of strains of Salmonella enteritidis phage type 4 (PT 4) isolated in England and Wales between 1967 and 1992. When applied to 39 isolates from humans, chickens, poultry products and feed, strains belonging to p...

journal_title：FEMS microbiology letters

authors： Powell NG,Threlfall EJ,Chart H,Rowe B

更新日期：1994-06-01 00:00:00

abstract：:Phycoerythrin-containing Synechococcus strains are unicellular cyanobacteria that are of great ecological importance in the marine environment. These organisms are known to be susceptible to infection by cyanophages (viruses that infect cyanobacteria). The infection cycle takes several hours and during this time the c...

journal_title：FEMS microbiology letters

authors： Shan J,Jia Y,Clokie MR,Mann NH

更新日期：2008-06-01 00:00:00