Abstract:
:A murine monoclonal antibody, designated MA-2G9, directed against outer surface protein A (OspA) of the Lyme disease spirochete, Borrelia burgdorferi, has been produced. Antibody MA-2G9, IgG1 subclass, was purified by affinity chromatography on protein G Sepharose column and used for purification of OspA antigen from Borrelia burgdorferi cell lysate. Epitope specificity was studied by Western immunoblotting, using several strains of B. burgdorferi and non-Lyme disease bacteria such as Treponema pallidum and B. hermsii. The MA-2G9 monoclonal antibody reacted specifically with recombinant OspA as well as with native OspA in sonicated B. burgdorferi strains. No reaction was observed with T. pallidum, Escherichia coli, Staphylococcus aureus and B. hermsii lysates. The MA-2G9 antibody also recognized the denatured form of OspA indicating that it is directed against sequential epitope and not conformational epitope.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Abolhassani M,Pavia CS,Dunn JJ,Chiao JWdoi
10.1016/0378-1097(93)90037-3subject
Has Abstractpub_date
1993-05-15 00:00:00pages
303-9issue
2-3eissn
0378-1097issn
1574-6968journal_volume
109pub_type
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