Expression of the landomycin biosynthetic gene cluster in a PKS mutant of Streptomyces fradiae is dependent on the coexpression of a putative transcriptional activator gene.

Abstract:

:The formation of landomycin A or one of its derivatives (5,6-anhydrolandomycin A) in a heterologous strain has never been achieved. It has now been made possible by the coexpression of a cosmid containing all biosynthetic genes necessary to produce landomycin A together with a pathway-specific regulatory gene. As host we used a polyketide synthase-defective mutant strain of Streptomyces fradiae Tü2717 which is not able to produce urdamycin A. Our results indicate that four glycosyltransferases are responsible for the formation of the hexasaccharide side chain of landomycin A.

journal_name

FEMS Microbiol Lett

authors

von Mulert U,Luzhetskyy A,Hofmann C,Mayer A,Bechthold A

doi

10.1016/S0378-1097(03)00861-9

keywords:

subject

Has Abstract

pub_date

2004-01-15 00:00:00

pages

91-7

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378109703008619

journal_volume

230

pub_type

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