Cloning of xylanase gene of Streptomyces flavogriseus in Escherichia coli and bacteriophage lambda-induced lysis for the release of cloned enzyme.


:The xylanase gene of Streptomyces flavogriseus was cloned in pUC8 plasmid and expressed in Escherichia coli lysogenic for lambda cI857. lambda-Induced lysis of E. coli at 42 degrees C allowed efficient release of cloned enzyme activity in extracellular environment. The xylanase gene was located in the 0.8-kb HindIII fragment and coded for 18,000 Mr xylanase.


FEMS Microbiol Lett


Srivastava R,Ali SS,Srivastava BS




Has Abstract


1991-03-01 00:00:00












  • The transcriptional regulator pool of the marine bacterium Rhodopirellula baltica SH 1T as revealed by whole genome comparisons.

    abstract::Rhodopirellula baltica (strain SH 1T) is a free-living marine representative of the phylogenetically independent and environmentally relevant phylum Planctomycetes. Little is known about the regulatory strategies of free-living bacteria with large (7.15 Mb) genomes. Therefore, a consistent, quantitative and qualitativ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lombardot T,Bauer M,Teeling H,Amann R,Glöckner FO

    更新日期:2005-01-01 00:00:00

  • Genetic evidence that hepA gene is involved in the normal deposition of the envelope of both heterocysts and akinetes in Anabaena variabilis ATCC 29413.

    abstract::The hepA gene in Anabaena sp. strain PCC 7120 is required for normal formation of the polysaccharide layer of the heterocyst envelope. A plasmid bearing hepA, interrupted by a neomycin-resistance cassette, was transferred by conjugation to wild type Anabaena variabilis ATCC 29413, so that the interrupted hepA gene rep...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Leganés F

    更新日期:1994-10-15 00:00:00

  • Roseovarius sp. strain 217: aerobic taurine dissimilation via acetate kinase and acetate-CoA ligase.

    abstract::The genome sequence of Roseovarius sp. strain 217 indicated that many pathway enzymes found in other organisms for the degradation of taurine are represented, but that a novel, apparently energy-dependent pathway is involved in the conversion of acetyl phosphate to acetyl CoA. Thus, an ABC transporter for taurine coul...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Baldock MI,Denger K,Smits TH,Cook AM

    更新日期:2007-06-01 00:00:00

  • Production of unmarked mutations in mycobacteria using site-specific recombination.

    abstract::Gene disruption experiments play an important role in the functional characterization of genes in mycobacteria and rely mostly on the use of one or two antibiotic resistance markers. We have developed a system for mycobacteria which features both the advantages of the use of antibiotic resistance markers for gene disr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Malaga W,Perez E,Guilhot C

    更新日期:2003-02-28 00:00:00

  • Construction of the first shuttle vectors for gene cloning and homologous recombination in Mycoplasma agalactiae.

    abstract::Mycoplasma agalactiae is a worldwide ruminant pathogen that causes significant economic losses by inflicting contagious agalactia in sheep and goats. The development of efficient control strategies requires a better understanding of the mycoplasma factors that promote successful infection. However, lack of genetic too...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Chopra-Dewasthaly R,Marenda M,Rosengarten R,Jechlinger W,Citti C

    更新日期:2005-12-01 00:00:00

  • The rhamnolipid stress response of Bacillus subtilis.

    abstract::Rhamnolipids are biosurfactants produced by the soil bacterium P seudomonas aeruginosa. In addition to their high industrial potential as surface-active molecules, rhamnolipids also have antimicrobial properties. In densely populated habitats, such as the soil, production of antimicrobial compounds is important to inh...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wecke T,Bauer T,Harth H,Mäder U,Mascher T

    更新日期:2011-10-01 00:00:00

  • Analysis of the role of the two flagella of Bradyrhizobium japonicum in competition for nodulation of soybean.

    abstract::Bradyrhizobium japonicum has two types of flagella. One has thin filaments consisting of the 33-kDa flagellins FliCI and FliCII (FliCI-II) and the other has thick filaments consisting of the 65-kDa flagellins FliC1, FliC2, FliC3, and FliC4 (FliC1-4). To investigate the roles of each flagellum in competition for nodula...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Althabegoiti MJ,Covelli JM,Pérez-Giménez J,Quelas JI,Mongiardini EJ,López MF,López-García SL,Lodeiro AR

    更新日期:2011-06-01 00:00:00

  • Description of new filamentous toxic Cyanobacteria (Oscillatoriales) colonizing the sulfidic periphyton mat in marine mangroves.

    abstract::In this multidisciplinary study, we combined morphological, physiological, and phylogenetic approaches to identify three dominant water bloom-forming Cyanobacteria in a tropical marine mangrove in Guadeloupe (French West Indies). Phylogenetic analysis based on 16S rRNA gene sequences place these marine Cyanobacteria i...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Guidi-Rontani C,Jean MR,Gonzalez-Rizzo S,Bolte-Kluge S,Gros O

    更新日期:2014-10-01 00:00:00

  • Microbiome characterization of poultry products based on the poultry part and production label.

    abstract::Poultry is one of the most consumed meats worldwide and there are many different types of poultry products available on the market for consumers. Many poultry producers provide a variety of labeled meats based on rearing system such as organic, free-range and antibiotic-free. However, these labels often can be mislead...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lee SI,Choi J,Daeschel DZ,Park SH

    更新日期:2019-05-01 00:00:00

  • Hydrocarbon utilization within a diesel-degrading bacterial consortium.

    abstract::Diesel fuel is a common environmental pollutant comprised of a large number of both aromatic and aliphatic hydrocarbons. The microbial degradation of individual hydrocarbons has been well characterized, however, the community dynamics within a system degrading a complex pollutant such as diesel fuel are still poorly u...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ciric L,Philp JC,Whiteley AS

    更新日期:2010-02-01 00:00:00

  • A new pullulan and a branched (1-->3)-, (1-->6)-linked beta-glucan from the lichenised ascomycete Teloschistes flavicans.

    abstract::The polysaccharides formed on hot alkaline extraction of the ascomycetous lichen Teloschistes flavicans were fractionated to give two glucans, which were characterised by methylation analysis and 1D and 2D NMR spectroscopy. One was a branched beta-glucan containing (1-->3) and (1-->6) linkages, a structure which is mo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Reis RA,Tischer CA,Gorin PA,Iacomini M

    更新日期:2002-04-23 00:00:00

  • Sequencing and characterization of a novel serine metalloprotease from Burkholderia pseudomallei.

    abstract::Burkholderia pseudomallei, a Gram-negative bacterium is found in the soil and water, mainly in Southeast Asia and Northern Australia. It is responsible for melioidosis in human and animals. The bacteria produce several potential virulent factors such as extracellular protease, hemolysin, lipase and lecithinase. The is...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lee MA,Liu Y

    更新日期:2000-11-01 00:00:00

  • Local hopping of IS3 elements into the A+T-rich part of the high-pathogenicity island in Yersinia enterocolitica 1B, O:8.

    abstract::The high-pathogenicity island (Yen HPI) of Yersinia enterocolitica biogroup (BG) 1B strains is associated with mouse virulence. Three repeated sequences are clustered on the A+T-rich part of the Yen HPI downstream of the fyuA yersiniabactin receptor gene in Y. enterocolitica O:8 strains WA-314 and 8081. In addition to...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Rakin A,Schubert S,Guilvout I,Carniel E,Heesemann J

    更新日期:2000-01-15 00:00:00

  • Esterase from the oil-degrading Acinetobacter lwoffii RAG-1: sequence analysis and over-expression in Escherichia coli.

    abstract::The est gene encoding an esterase from Acinetobacter lwoffii RAG-1 was cloned into E. coli under the control of the PL promoter of the phage lambda. The N-terminal sequence of the first 20 amino acids of the heterologous expressed esterase corresponded to that obtained from the nucleotide sequence. Antibodies prepared...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Alon RN,Gutnick DL

    更新日期:1993-09-15 00:00:00

  • RNA transcript sequencing reveals inorganic sulfur compound oxidation pathways in the acidophile Acidithiobacillus ferrivorans.

    abstract::Acidithiobacillus ferrivorans is an acidophile implicated in low-temperature biomining for the recovery of metals from sulfide minerals. Acidithiobacillus ferrivorans obtains its energy from the oxidation of inorganic sulfur compounds, and genes encoding several alternative pathways have been identified. Next-generati...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Christel S,Fridlund J,Buetti-Dinh A,Buck M,Watkin EL,Dopson M

    更新日期:2016-04-01 00:00:00

  • Distribution of the IS elements ISS1 and IS904 in lactococci.

    abstract::A broad distribution of the lactococcal IS elements ISS1 [1] and IS904 [2] in several lactococcal plasmids and chromosomal DNA was observed. Hybridization of the ISS1 and IS904 oligonucleotide gene probes with DNA of lactococcal phages showed that none of these tested bacteriophages contained one of the IS elements. O...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Schäfer A,Jahns A,Geis A,Teuber M

    更新日期:1991-05-15 00:00:00

  • Accumulation of ppGpp in Streptococcus pyogenes and Streptococcus rattus following amino acid starvation.

    abstract::We have re-examined the stringent response of Streptococcus rattus and Streptococcus pyogenes, two organisms that had originally been reported not to accumulate ppGpp following amino acid deprivation. We conclude that ppGpp does accumulate when S. rattus and S. pyogenes are deprived of isoleucine by mupirocin addition...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Whitehead KE,Webber GM,England RR

    更新日期:1998-02-01 00:00:00

  • Characterization of human humoral responses to the major outer membrane protein and OMP2 of Chlamydophila pneumoniae.

    abstract::Chlamydophila pneumoniae infection is associated with a range of diseases including pneumonia, asthma and heart disease. Although an obligate intracellular pathogen, high levels of antigen-specific antibody are induced and serology is frequently used to diagnose these infections. Proteins targeted by the humoral respo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cunningham AF,Ward ME

    更新日期:2003-10-10 00:00:00

  • Acquisition of plasmin activity and induction of arachidonic acid release by Streptococcus suis in contact with human brain microvascular endothelial cells.

    abstract::Infections caused by Streptococcus suis, a major swine pathogen, include meningitis, arthritis, pneumonia and septicaemia. In this study, we investigated interactions that may occur between human brain microvascular endothelial cells (HBMEC), the main constituent of the blood-brain barrier, and S. suis. We show that S...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jobin MC,Fortin J,Willson PJ,Gottschalk M,Grenier D

    更新日期:2005-11-01 00:00:00

  • The adaptation and resistance of Clostridium aminophilum F to the butyrivibriocin-like substance of Butyrivibrio fibrisolvens JL5 and monensin.

    abstract::When the amino acid-fermenting bacterium Clostridium aminophilum F was inoculated into media containing 1 microM monensin or a bacteriocin-like inhibitory substance (BLIS) from Butyrivibrio fibrisolvens JL5, the cultures lagged and growth was not observed for more than 12 h. The monensin- and BLIS-treated cultures eve...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Rychlik JL,Russell JB

    更新日期:2002-03-19 00:00:00

  • Efflux of choline and glycine betaine from osmoregulating cells of Escherichia coli.

    abstract::We present evidence that glycine betaine (betaine) which was synthesized from choline was excreted and reaccumulated in osmoregulating cells of Escherichia coli. Choline which was accumulated in bet mutants defective in betaine synthesis was shown to be excreted in response to betaine uptake. Our data suggest that E. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lamark T,Styrvold OB,Strøm AR

    更新日期:1992-09-15 00:00:00

  • Sphingomonas astaxanthinifaciens sp. nov., a novel astaxanthin-producing bacterium of the family Sphingomonadaceae isolated from Misasa, Tottori, Japan.

    abstract::A red-pigmented, Gram-negative, motile, strictly aerobic, mesophilic, oval- or short rod-shaped bacterium (TDMA-17(T)) was isolated from fresh water collected at Misasa, a radioactive site in Japan. TDMA-17(T) was slightly tolerant against gamma-ray irradiation, and effectively produced carotenoids (2.8 mg g(-1) dry c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Asker D,Beppu T,Ueda K

    更新日期:2007-08-01 00:00:00

  • Cloning and analysis of the polyhydroxyalkanoic acid synthase gene from an Acinetobacter sp.: evidence that the gene is both plasmid and chromosomally located.

    abstract::The polyhydroxyalkanoic acid (PHA) synthase gene (phaCAc) of a species of Acinetobacter isolated from an activated sludge treatment plant was cloned by heterologous complementation in a poly-beta-hydroxybutyrate (PHB) negative mutant of Alcaligenes eutrophus. Nucleotide sequence analysis of phaCAc revealed an open rea...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Schembri MA,Bayly RC,Davies JK

    更新日期:1994-05-01 00:00:00

  • Biodegradation of phenanthrene by Alcaligenes sp. strain PPH: partial purification and characterization of 1-hydroxy-2-naphthoic acid hydroxylase.

    abstract::Alcaligenes sp. strain PPH degrades phenanthrene via 1-hydroxy-2-naphthoic acid (1-H2NA), 1,2-dihydroxynaphthalene (1,2-DHN), salicylic acid and catechol. Enzyme activity versus growth profile and heat stability studies suggested the presence of two distinct hydroxylases, namely 1-hydroxy-2-naphthoic acid hydroxylase ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Deveryshetty J,Phale PS

    更新日期:2010-10-01 00:00:00

  • Involvement of the mpo operon in resistance to class IIa bacteriocins in Listeria monocytogenes.

    abstract::High resistance to class IIa bacteriocins in Listeria monocytogenes has been clearly linked to lack of expression of the mptACD operon, encoding the EIIt Man mannose PTS permease. Also, intermediate resistance has been associated with membrane phospholipid modifications in the spontaneous mutants L. monocytogenes B73-...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Arous S,Dalet K,Héchard Y

    更新日期:2004-09-01 00:00:00

  • Overproduction of L-cysteine and L-cystine by expression of genes for feedback inhibition-insensitive serine acetyltransferase from Arabidopsis thaliana in Escherichia coli.

    abstract::Two cDNAs encoding feedback inhibition-insensitive serine acetyltransferases of Arabidopsis thaliana were expressed in the chromosomal serine acetyltransferase-deficient and L-cysteine non-utilizing Escherichia coli strain JM39-8. The transformants produced 1600 to 1700 mg l(-1) of L-cysteine and L-cystine from glucos...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Takagi H,Awano N,Kobayashi S,Noji M,Saito K,Nakamori S

    更新日期:1999-10-15 00:00:00

  • Detection of hepatitis B virus DNA by polymerase chain reaction in HBsAg negative Senegalese patients suffering from cirrhosis or primary liver cancer.

    abstract::The polymerase chain reaction was used to search for hepatitis B virus (HBV)-DNA sequences in the sera of HBsAg-negative Senegalese patients suffering from liver cirrhosis or liver cancer. Amplified HBV-DNA sequences were detected by hybridization with a digoxigenin-labelled HBV-DNA probe. HBV-DNA was detected in 17% ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Coursaget P,Le Cann P,Leboulleux D,Diop MT,Bao O,Coll AM

    更新日期:1991-09-15 00:00:00

  • Comparison of transcription of multiple genes at three developmental stages of the plant pathogen Sclerotinia sclerotiorum.

    abstract::The ascomycete Sclerotinia sclerotiorum is a plant pathogen with a very broad host range. In order to identify and characterize genes involved in S. sclerotiorum infection of Brassica napus (canola), expressed sequence tags (ESTs) were examined from libraries prepared from three tissues: complex appressorium (infectio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sexton AC,Cozijnsen AJ,Keniry A,Jewell E,Love CG,Batley J,Edwards D,Howlett BJ

    更新日期:2006-05-01 00:00:00

  • Cellular transformation by human T-cell leukemia virus type I.

    abstract::Human T-cell leukemia virus type I (HTLV-I) is an oncogenic retrovirus that was isolated in 1980 from a patient with adult T-cell leukemia. From the numerous experiments using infected patient T-cells, transgenic mice and tissue culture transformation assays, the Tax protein has been determined to be the transforming ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Ressler S,Connor LM,Marriott SJ

    更新日期:1996-07-01 00:00:00

  • Sucrose favors Pseudomonas aeruginosa pellicle production through the extracytoplasmic function sigma factor SigX.

    abstract::Pseudomonas aeruginosa biofilm formation was increased by addition of sucrose to Luria-Bertani medium, whereas addition of NaCl to a final similar osmolarity and use of maltose instead of sucrose, were ineffective. In a previous study, we showed that the extracytoplasmic sigma factor SigX is activated in the presence ...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Bouffartigues E,Duchesne R,Bazire A,Simon M,Maillot O,Dufour A,Feuilloley M,Orange N,Chevalier S

    更新日期:2014-07-01 00:00:00