Cloning of xylanase gene of Streptomyces flavogriseus in Escherichia coli and bacteriophage lambda-induced lysis for the release of cloned enzyme.

Abstract:

:The xylanase gene of Streptomyces flavogriseus was cloned in pUC8 plasmid and expressed in Escherichia coli lysogenic for lambda cI857. lambda-Induced lysis of E. coli at 42 degrees C allowed efficient release of cloned enzyme activity in extracellular environment. The xylanase gene was located in the 0.8-kb HindIII fragment and coded for 18,000 Mr xylanase.

journal_name

FEMS Microbiol Lett

authors

Srivastava R,Ali SS,Srivastava BS

doi

10.1016/0378-1097(91)90158-7

subject

Has Abstract

pub_date

1991-03-01 00:00:00

pages

201-5

issue

2-3

eissn

0378-1097

issn

1574-6968

journal_volume

62

pub_type

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