Diversity of pigmented Gram-positive bacteria associated with marine macroalgae from Antarctica.


:Little is known about the diversity and roles of Gram-positive and pigmented bacteria in Antarctic environments, especially those associated with marine macroorganisms. This work is the first study about the diversity and antimicrobial activity of culturable pigmented Gram-positive bacteria associated with marine Antarctic macroalgae. A total of 31 pigmented Gram-positive strains were isolated from the surface of six species of macroalgae collected in the King George Island, South Shetland Islands. On the basis of 16S rRNA gene sequence similarities ≥99%, 18 phylotypes were defined, which were clustered into 11 genera of Actinobacteria (Agrococcus, Arthrobacter, Brachybacterium, Citricoccus, Kocuria, Labedella, Microbacterium, Micrococcus, Rhodococcus, Salinibacterium and Sanguibacter) and one genus of the Firmicutes (Staphylococcus). It was found that five isolates displayed antimicrobial activity against a set of macroalgae-associated bacteria. The active isolates were phylogenetically related to Agrococcus baldri, Brachybacterium rhamnosum, Citricoccus zhacaiensis and Kocuria palustris. The results indicate that a diverse community of pigmented Gram-positive bacteria is associated with Antartic macroalgae and suggest its potential as a promising source of antimicrobial and pigmented natural compounds.


FEMS Microbiol Lett


Leiva S,Alvarado P,Huang Y,Wang J,Garrido I




Has Abstract


2015-12-01 00:00:00














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    abstract::An exo-polygalacturonase (EC was purified to apparent homogeneity from cultures of Fusarium oxysporum f.sp. lycopersici on synthetic medium supplemented with citrus pectin, using preparative isoelectric focusing. The enzyme, denominated PG2, had an apparent M(r) of 74000 Da upon SDS-PAGE. The pI of the main ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Di Pietro A,Roncero MI

    更新日期:1996-12-01 00:00:00

  • Molecular host mimicry and manipulation in bacterial symbionts.

    abstract::It is common among intracellular bacterial pathogens to use eukaryotic-like proteins that mimic and manipulate host cellular processes to promote colonization and intracellular survival. Eukaryotic-like proteins are bacterial proteins with domains that are rare in bacteria, and known to function in the context of a eu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Frank AC

    更新日期:2019-02-01 00:00:00

  • Adhesive properties of the purified plasminogen activator Pla of Yersinia pestis.

    abstract::The beta-barrel outer membrane protease Pla from Yersinia pestis is an important virulence factor in plague and enables initiation of the bubonic plague. Pla is a multifunctional protease whose expression also enhances bacterial adherence to extracellular matrix. It has remained uncertain whether the increase in cellu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lobo LA

    更新日期:2006-09-01 00:00:00

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Loh JT,Torres VJ,Algood HM,McClain MS,Cover TL

    更新日期:2008-12-01 00:00:00

  • Monitoring intracellular redox conditions in the endoplasmic reticulum of living yeasts.

    abstract::Methods for in vivo monitoring of redox changes in different cellular compartments have been developed in recent years, and are mostly based on redox-sensitive variants of the green fluorescent protein (GFP). However, due to the thermodynamic stability of the introduced reactive disulfide bond, these sensors are limit...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Delic M,Mattanovich D,Gasser B

    更新日期:2010-05-01 00:00:00

  • Pre-exposure to UV irradiation increases the transfer frequency of the IncJ conjugative transposon-like elements R391, R392, R705, R706, R997 and pMERPH and is recA+ dependent.

    abstract::The enteric conjugative transposon-like IncJ elements R391, R392, R705, R706 and pMERPH, all demonstrated increased conjugative transfer upon UV irradiation. The transfer frequency increased on average from its basal rate of 10(-5) to 10(-3) per recipient, upon pre-exposure to UV irradiation. However, the transfer fre...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: McGrath BM,O'Halloran JA,Pembroke JT

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gowher H,Ehrlich KC,Jeltsch A

    更新日期:2001-11-27 00:00:00

  • Neisseria meningitidis transferrin-binding protein 1 expressed in Escherichia coli is surface exposed and binds human transferrin.

    abstract::A gene library of Neisseria meningitidis B15 P1.16 DNA was established in lambda Zap II and clones containing DNA encoding transferrin binding protein 1 (TBP-1) identified following hybridisation with a 63-bp DNA probe based on the codon assignment for the first 21 N-terminal amino acids of TBP-1. Sequencing of the cl...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Palmer HM,Powell NB,Ala'Aldeen DA,Wilton J,Borriello SP

    更新日期:1993-06-15 00:00:00

  • Co-expression of Bacillus thuringiensis Cry4Ba and Cyt2Aa2 in Escherichia coli revealed high synergism against Aedes aegypti and Culex quinquefasciatus larvae.

    abstract::Cry4Ba is a delta-endotoxin produced by Bacillus thuringiensis subsp. israelensis and Cyt2Aa2 is a cytolytic delta-endotoxin produced by B. thuringiensis subsp. darmstadiensis. Cry4Ba produced in Escherichia coli was toxic to Aedes aegypti larvae (LC(50)=140 ng ml(-1)) but virtually inactive to Culex quinquefasciatus ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Promdonkoy B,Promdonkoy P,Panyim S

    更新日期:2005-11-01 00:00:00

  • Modular structure of the Rhizobium meliloti DctB protein.

    abstract::To investigate the modular structure of the Rhizobium meliloti dicarboxylic acid sensor protein, DctB, three truncated DctB proteins (DctB4, DctB5 and DctB4G) were constructed, overproduced in Escherichia coli and purified. The DctB4G protein was composed of 446 amino acids of the DctB C-terminus and displayed strong ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Giblin L,Archdeacon J,O'Gara F

    更新日期:1996-05-15 00:00:00

  • Molecular cloning and phylogenetic analysis of the small cytoplasmic RNA from Listeria monocytogenes.

    abstract::A molecular cloning strategy has been designed to isolate the gene that encodes the small cytoplasmic RNA (scRNA) component of bacterial signal recognition particles. Using this strategy a putative Listeria monocytogenes scRNA lambda gt11 recombinant clone was isolated. A previously described complementation assay dev...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Barry T,Kelly M,Glynn B,Peden J

    更新日期:1999-04-01 00:00:00

  • Conjugative transfer of a shuttle plasmid from Escherichia coli to Mycobacterium smegmatis [corrected].

    abstract::The chimeric plasmid pMY10 containing the origin of replication of pAL5000, the origin of replication of pBR322, the origin of transfer of pRK2 and a kanamycin resistance gene was constructed and successfully transferred by conjugation from Escherichia coli harbouring the helper plasmid pRK4.24 into Mycobacterium smeg...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lazraq R,Clavel-Sérès S,David HL,Roulland-Dussoix D

    更新日期:1990-05-01 00:00:00

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    abstract::Pythium drechsleri Paul was described as a new species from soil samples taken in a salt-marsh of Arzew, Algeria [Paul, B. (1988) Une nouvelle espèce de Pythium isolée d'une saline de l'ouest Algérien. Cryptogam. Mycol. 9, 325-333]. The name of the fungus, P. drechsleri, is a nomen invalidum, as it is a later homonym ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Paul B

    更新日期:2000-02-01 00:00:00

  • Functional expression of the glutamate uptake system from Corynebacterium glutamicum in Escherichia coli.

    abstract::Glutamate uptake in the Gram-positive Corynebacterium glutamicum is mediated via a binding protein-dependent transport system, which is encoded by the gluABCD gene cluster. Cloning of these genes in an expression vector and subsequent transformation of the resulting plasmid allows different strains of the Gram-negativ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Burkovski A,Krämer R

    更新日期:1995-04-01 00:00:00

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    abstract::During protein synthesis, elongation factor G (EFG) participates at the steps of translocation and ribosome recycling. Fusidic acid (FA) is a bacteriostatic antibiotic, which traps EFG on ribosomes, stalling them on mRNAs. How the bacterial susceptibility to FA is determined, and which of the two functions of EFG (tra...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ayyub SA,Lahry K,Dobriyal D,Mondal S,Varshney U

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    abstract::A complementary DNA of the Aspergillus nidulans chsB gene encoding chitin synthase, an essential gene for hyphal growth, was obtained by RT-PCR and expressed in Saccharomyces cerevisiae by using the GAL1 promoter in a multicopy plasmid. The biochemical characteristics of chitin synthase B (ChsB) expressed in S. cerevi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Tatsuno K,Yamada-Okabe H,Takagi M,Arisawa M,Sudoh M

    更新日期:1997-04-15 00:00:00

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    abstract::The lipoate acetyltransferase (E2p) subunits of the pyruvate dehydrogenase (PDH) complex of Escherichia coli have three tandemly repeated lipoyl domains, although net deletions of one or two has no apparent effect on the activity of the purified complexes. Plasmids containing IPTG-inducible aceEF-lpd operons, which en...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Machado RS,Clark DP,Guest JR

    更新日期:1992-12-15 00:00:00

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    abstract::Pseudomonas chlororaphis PA23 is a biocontrol agent that, in addition to producing antifungal compounds, synthesizes polyhydroxyalkanoate (PHA) polymers as a carbon and energy sink. Quorum sensing (QS) and the anaerobic regulator (ANR) are required for PA23-mediated fungal suppression; however, the role of these regul...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mohanan N,Gislason A,Sharma PK,Ghergab A,Plouffe J,Levin DB,de Kievit T

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Tarraran L,Mazzoli R

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lee YK,Chang HH,Lee HJ,Park H,Lee KH,Joe MH

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Smith AM,Yan H,Groves N,Dalla Pozza T,Walker MJ

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Bullerjahn GS,Jensen TC,Sherman DM,Sherman LA

    更新日期:1990-01-15 00:00:00

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Zanoelo FF,Polizeli Mde L,Terenzi HF,Jorge JA

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Akimana C,Lafontaine ER

    更新日期:2007-06-01 00:00:00

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gates AJ,Hughes RO,Sharp SR,Millington PD,Nilavongse A,Cole JA,Leach ER,Jepson B,Richardson DJ,Butler CS

    更新日期:2003-03-28 00:00:00

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Chen Y,Tan H,Qin Z

    更新日期:2013-12-01 00:00:00

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    abstract::Antibody to Escherichia coli O157 lipopolysaccharide was detected in the sera of healthy individuals more frequently in Southern Thailand than in Japan. The result suggested possible exposure of Thai people to E. coli O157. E. coli O157:H7 or O157:H(-) was isolated from four of 95 retail beef and one of 55 bovine fece...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Vuddhakul V,Patararungrong N,Pungrasamee P,Jitsurong S,Morigaki T,Asai N,Nishibuchi M

    更新日期:2000-01-15 00:00:00

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Ward VCA,Chatzivasileiou AO,Stephanopoulos G

    更新日期:2018-05-01 00:00:00

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    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Rodríguez-Sáiz M,Lembo M,Bertetti L,Muraca R,Velasco J,Malcangi A,de la Fuente JL,Barredo JL

    更新日期:2004-06-01 00:00:00