Direct profiling of the yeast dynamics in wine fermentations.

Abstract:

:We present a method to directly characterize the yeast diversity present in wine fermentations by employing denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 26S ribosomal RNA (rRNA) genes. PCR-DGGE of a portion of the 26S rRNA gene was shown to distinguish most yeast genera associated with the production of wine. With this method the microbial dynamics in several model wine fermentations were profiled. PCR-DGGE provided a qualitative assessment of the yeast diversity in these fermentations accurately identifying populations as low as 1000 cells ml(-1). PCR-DGGE represents an attractive alternative to traditional plating schemes for analysis of the microbial successions inherent in the fermentation of wine.

journal_name

FEMS Microbiol Lett

authors

Cocolin L,Bisson LF,Mills DA

doi

10.1111/j.1574-6968.2000.tb09210.x

keywords:

subject

Has Abstract

pub_date

2000-08-01 00:00:00

pages

81-7

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(00)00257-3

journal_volume

189

pub_type

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