Abstract:
:We present a method to directly characterize the yeast diversity present in wine fermentations by employing denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 26S ribosomal RNA (rRNA) genes. PCR-DGGE of a portion of the 26S rRNA gene was shown to distinguish most yeast genera associated with the production of wine. With this method the microbial dynamics in several model wine fermentations were profiled. PCR-DGGE provided a qualitative assessment of the yeast diversity in these fermentations accurately identifying populations as low as 1000 cells ml(-1). PCR-DGGE represents an attractive alternative to traditional plating schemes for analysis of the microbial successions inherent in the fermentation of wine.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Cocolin L,Bisson LF,Mills DAdoi
10.1111/j.1574-6968.2000.tb09210.xkeywords:
subject
Has Abstractpub_date
2000-08-01 00:00:00pages
81-7issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(00)00257-3journal_volume
189pub_type
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