Abstract:
:The RS1 element associated with Vibrio cholerae CTX phi prophage was cloned from an E1 Tor biotype Vibrio cholerae strain. We used the recA- vaccine strain Peru-15, that lacks the target for RS-mediated site-specific integration, to show that RS1 promotes autonomous replication of a suicide vector. A linker insertion in the rstR open reading frame abolished autonomous replication in Peru-15 but not in a strain containing an RS1 in the chromosome. An AT-rich region containing cis-acting elements involved in autonomous replication was identified by deletion. This region was sufficient to support autonomous replication in a strain containing an RS1 in the chromosome. DNA sequence analysis of a region present in RS1 and not RS2 revealed the presence of putative binding sites for host proteins involved in plasmid replication. These results indicate that RS1 contains a replicon distinct from RS2 which could be involved in replicative recombination events associated with tandem amplification of the CTX element.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Campos J,Fando R,Silva A,Rodriguez BL,Benitez JAdoi
10.1111/j.1574-6968.1998.tb13079.xsubject
Has Abstractpub_date
1998-07-01 00:00:00pages
141-7issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(98)00208-0journal_volume
164pub_type
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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