Abstract：
:Clostridium difficile, a Gram-positive spore-forming anaerobe, causes infections in humans ranging from mild diarrhoeal to potentially life-threatening pseudomembranous colitis. The availability of genomic information for a range of C. difficile strains affords researchers the opportunity to better understand not only the evolution of these organisms but also their basic physiology and biochemistry. We used proteomics to characterize the insoluble subproteome of C. difficile strain 630. Gel-based LC-MS analysis led to the identification of 2298 peptides; provalt analysis with a false discovery rate set at 1% concatenated this list to 560 unique peptides, resulting in 107 proteins being positively identified. These were functionally classified and physiochemically characterized and pathway reconstruction identified a variety of central anaerobic metabolic pathways, including glycolysis, mixed acid fermentation and short-chain fatty acid metabolism. Additionally, the metabolism of a variety of amino acids was apparent, including the reductive branch of the leucine fermentation pathway, from which we identified seven of the eight enzymes. Increasing proteomics data sets should - in conjunction with other 'omic' technologies - allow the construction of models for 'normal' metabolism in C. difficile 630. This would be a significant initial step towards a full systems understanding of this clinically important microorganism.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Jain S,Graham RL,McMullan G,Ternan NGdoi
10.1111/j.1574-6968.2010.02111.xsubject
Has Abstractpub_date
2010-11-01 00:00:00pages
151-9issue
2eissn
0378-1097issn
1574-6968journal_volume
312pub_type
杂志文章abstract：:In Bacillus subtilis, carbon catabolite control is mediated by the regulatory protein CcpA. In addition to loss of catabolite repression, ccpA mutants exhibit a severe growth defect. They are not able to grow with glucose and ammonium as single sources of carbon and nitrogen, respectively. Only ccpA mutant strains car...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2001.tb10830.x
更新日期：2001-09-11 00:00:00
abstract：:Enterococcus faecium has emerged as an important cause of nosocomial infections over the last two decades. We recently demonstrated collagen type I (CI) as a common adherence target for some E. faecium isolates and a significant correlation was found to exist between acm-mediated CI adherence and clinical origin. Here...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2009.01806.x
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abstract：:Haemin (iron protoporphyrin IX) is an essential growth factor for the periodontal pathogen. Porphyromonas gingivalis. Iron protoporphyrin IX (IPP IX) binding to the avirulent P. gingivalis beige variant (W50/BE1) and the black-pigmenting parent wild-type strain W50 was quantified. W50/BE1 grown in a chemostat under ha...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1996.tb08364.x
更新日期：1996-07-15 00:00:00
abstract：:Two-component systems are widespread in nature and constitute the most common mechanism of transmembrane signal transduction in bacteria. Recently, a functionally active two-component system consisting of malS and malR genes possibly involved in the expression of a C4-dicarboxylate transporter system (dctAB operon) wa...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2009.01589.x
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1997.tb12566.x
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2001.tb10735.x
更新日期：2001-07-10 00:00:00
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2001.tb10902.x
更新日期：2001-11-13 00:00:00
abstract：:The significance of the chronicled role of the yeast transcription factor Adr1p in regulating ETR1 was examined in wild type and isogenic adr1Delta mutant cells. An ETR1-lacZ reporter construct was used to verify Adr1p-dependent gene expression. On solid glycerol medium containing X-gal, wild-type cells expressing the...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
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更新日期：2009-08-01 00:00:00
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1993.tb06335.x
更新日期：1993-07-01 00:00:00
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2009.01639.x
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/j.femsle.2005.06.050
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/0378-1097(90)90307-c
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abstract：:Alignment of published 16S rRNA sequences allowed the definition of a pair of oligonucleotides suitable for polymerase chain reaction (PCR). Using this pair of PCR primers, several mycoplasmas including the four human parasites Mycoplasma genitalium, M. hominis, M. salivarium and M. orale were detected. This DNA ampli...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/0378-1097(91)90467-o
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1997.tb10314.x
更新日期：1997-04-01 00:00:00
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2002.tb11105.x
更新日期：2002-03-19 00:00:00
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2008.01449.x
更新日期：2009-02-01 00:00:00
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1093/femsle/fny021
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/j.femsle.2004.08.007
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journal_title：FEMS microbiology letters
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doi：10.1016/0378-1097(92)90147-g
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2011.02223.x
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abstract：:Currently, interest in phage science is on the rise again as this subject remains largely unexplored, and its potential diversity mainly untapped. Here, we present phage vB_BsuP-Goe1, a new isolate that infects Bacillus subtilis The phage forms round plaques with a matt outline on agar plates containing a B. subtilis ...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1093/femsle/fnw208
更新日期：2016-10-01 00:00:00
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/1574-6968.12121
更新日期：2013-05-01 00:00:00
abstract：:An open reading frame located in the tyrB-uvrA intergenic region of the Escherichia coli MG1655 chromosome was identified as encoding the class B acid phosphatase of this species on the basis of cloning and expression experiments. A protocol for purification of the enzyme (named AphA) was developed, and its properties...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1997.tb10192.x
更新日期：1997-01-15 00:00:00
abstract：:Cells of the pel1 mutant of Saccharomyces cerevisiae were found to contain an extremely low content of cardiolipin, a decreased level of phosphatidylcholine and an increased level of phosphatidylinositol. Disruption of the PEL1 gene in cells containing a null mutation in the CHO1 gene was lethal. Despite its putative ...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1996.tb08312.x
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/S0378-1097(03)00778-X
更新日期：2003-12-05 00:00:00
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journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2000.tb09164.x
更新日期：2000-07-01 00:00:00
abstract：:The sequence of 1383 nucleotides of the DNA encoding 16S rDNA was determined for strains of human intestinal spirochaetes, comprising an unnamed isolate and "Brachyspira aalborgi" NCTC 11492. A phylogenetic tree was inferred from aligned sequence comparisons between the intestinal spirochaetes, representatives of the ...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1994.tb06790.x
更新日期：1994-04-15 00:00:00
abstract：:The presence of cholesteryl glucosides and high levels of lysophospholipids are elements making the cell wall of Helicobacter pylori unique. In this study, we have investigated the relationship between lysophospholipid content and cholesteryl glucoside composition of variants of 6 clinical isolates. The samples were c...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/j.femsle.2005.01.030
更新日期：2005-03-01 00:00:00
abstract：:Using pulsed-field gel electrophoresis (PFGE), nine profile types were identified in XbaI digests of a panel of strains of Salmonella enteritidis phage type 4 (PT 4) isolated in England and Wales between 1967 and 1992. When applied to 39 isolates from humans, chickens, poultry products and feed, strains belonging to p...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1994.tb06888.x
更新日期：1994-06-01 00:00:00
