H4 histone in the macronucleus of Blepharisma japonicum (Protozoa, Ciliophora, Heterotrichida).

Abstract:

:Two clones, obtained by polymerase chain reaction from macronuclear DNA of the unicellular ciliated protist Blepharisma japonicum, were isolated and sequenced. They correspond to fragments of two different putative H4 histone genes. The existence of multiple H4 histone genes was also suggested by Southern blot hybridisation experiments employing one of the obtained clones as a probe. Two B. japonicum H4 protein fragments, which were directly sequenced, show differences in the amino acid sequences too. The comparison of the obtained B. japonicum H4 partial amino acid sequences with each other, and with H4 from other ciliates and from representative microbial and multicellular organisms, highlights the larger histone heterogeneity of lower eukaryotes compared to that observed in higher organisms.

journal_name

FEMS Microbiol Lett

authors

Salvini M,Bini E,Santucci A,Batistoni R

doi

10.1111/j.1574-6968.1997.tb10314.x

subject

Has Abstract

pub_date

1997-04-01 00:00:00

pages

93-8

issue

1

eissn

0378-1097

issn

1574-6968

pii

S037810979700061X

journal_volume

149

pub_type

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