Abstract:
:The gene encoding Aquifex aeolicus (Aae) DNA polymerase was expressed under the control of the trp promoter on a high-copy plasmid, pTRPNS, in Escherichia coli. The expressed enzyme was purified 11-fold with a 13.8% yield and a specific activity of 2268.3 U mg(-1). The optimum pH of the enzyme was 6.8-7.2. The optimal concentrations of KCl and Mg(2+) were 20-30 mM and 4-5 mM, respectively. Aae DNA polymerase contained a double-strand-dependent 3'-->5' proofreading exonuclease activity but lacked any detectable 5'-->3' exonuclease activity.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Chang JR,Choi JJ,Kim HK,Kwon STdoi
10.1111/j.1574-6968.2001.tb10735.xkeywords:
subject
Has Abstractpub_date
2001-07-10 00:00:00pages
73-7issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(01)00248-8journal_volume
201pub_type
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