Cell wall constituents of Microcystis sp. PCC 7806.


:Cell walls of Microcystis sp. PCC 7806 were purified from cell homogenates by sucrose density centrifugation and Triton X-100 extraction. The outer membrane contained carotenoids, two major peptidoglycan-associated proteins (Mr 49,000 and 52,000), and lipopolysaccharide (LPS) as indicated by the presence of 3-hydroxy fatty acids (3-OH-14:0, 3-OH-16:0, 3-OH-18:0), 4-oxo-18:0 fatty acid, and GlcN as lipid A components in addition to rare O-methyl sugars (2-O-methyl-6-deoxyhexoses I and II). The peptidoglycan (A1 gamma-type) was found to be covalently linked to a wall polysaccharide composed of GlcN, ManN, Man, Glc, and phosphate.


FEMS Microbiol Lett


Jürgens UJ,Martin C,Weckesser J




Has Abstract


1989-11-01 00:00:00












  • Cloning, sequence analysis and heterologous expression of the Myrothecium gramineum orotidine-5'-monophosphate decarboxylase gene.

    abstract::A 2918 bp sequence coding for the orotidine-5'-monophosphate decarboxylase enzyme (OMPD) was isolated from the genome of Myrothecium gramineum. This sequence was analysed and, remarkably, it is the first OMPD gene of a Sordariomycete that has an intron. The gene codes for an enzyme of 282 amino acids. The nucleotide s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: De Maeseneire SL,De Groeve MR,Dauvrin T,De Mey M,Soetaert W,Vandamme EJ

    更新日期:2006-08-01 00:00:00

  • Isozyme and morphological characteristics of the anaerobic fungus Piromyces mae isolated from the duodenum, rumen and faeces of sheep.

    abstract::Isolates of anaerobic fungi obtained from the rumen, duodenum and faeces of sheep were identified as Piromyces mae based on their morphological characteristics observed using light microscopy. There was no significant morphological variation among the isolates of P. mae from the rumen, duodenum and faeces. Isozymes of...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wong MV,Ho YW,Tan SG,Abdullah N,Jalaludin S

    更新日期:1995-12-01 00:00:00

  • Comparison of the thickening properties of four Lactobacillus delbrueckii subsp. bulgaricus strains and physicochemical characterization of their exopolysaccharides.

    abstract::It is now well established that physicochemical properties of exopolysaccharides (EPS) can vary between strains of a given species and according to growth conditions. The EPS production of four strains of Lactobacillus bulgaricus was monitored during growth in milk and in a chemically defined media. All strains, inclu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Petry S,Furlan S,Waghorne E,Saulnier L,Cerning dagger J,Maguin E

    更新日期:2003-04-25 00:00:00

  • Analysis of CcpA mutations defective in carbon catabolite repression in Bacillus megaterium.

    abstract::Five mutations in ccpA of Bacillus megaterium with impaired functions were analysed for carbon catabolite repression. The phenotypes support the hypothesis that CcpA assumes a PurR/LacI fold. The completely inactive mutants CcpA119GE and CcpA326am cause alterations which are incompatible with that fold. A mutation wit...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kraus A,Hillen W

    更新日期:1997-08-01 00:00:00

  • Glanders: off to the races with Burkholderia mallei.

    abstract::Burkholderia mallei, the etiologic agent of the disease known as glanders, is primarily a disease affecting horses and is transmitted to humans by direct contact with infected animals. The use of B. mallei as a biological weapon has been reported and currently, there is no vaccine available for either humans or animal...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Whitlock GC,Estes DM,Torres AG

    更新日期:2007-12-01 00:00:00

  • Non-radioactive restriction fragment length polymorphism (RFLP) typing of Clostridium difficile.

    abstract::A typing method for Clostridium difficile based on restriction fragment length polymorphisms (RFLP) is described. The technique utilizes commercially available Escherichia coli ribosomal ribonucleic acid (rRNA) as probe material. Probe labelling, hybridization and detection was performed using the Enhanced Chemilumine...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Bowman RA,O'Neill GL,Riley TV

    更新日期:1991-04-15 00:00:00

  • High-quality mutant libraries of Xanthomonas oryzae pv. oryzae and X. campestris pv. campestris generated by an efficient transposon mutagenesis system.

    abstract::A novel transposon mutagenesis system for the phytopathogenic bacteria Xanthomonas oryzae pv. oryzae (Xoo) and X. campestris pv. campestris (Xcc) was developed using a Tn5-based transposome. A highly efficient transformation up to 10(6) transformants per microg transposon DNA was obtained. Southern blot and thermal as...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sun Q,Wu W,Qian W,Hu J,Fang R,He C

    更新日期:2003-09-12 00:00:00

  • Epitopes of serogroup B Neisseria meningitidis analysed in vitro and directly from cerebrospinal fluid.

    abstract::Two type B15 P1.16 strains of Neisseria meningitidis were examined by immunogold electron microscopy for accessibility of two outer membrane protein (OMPs) to monoclonal antibodies. Both strains exhibited cell-to-cell variation of one epitope of the Class 3 OMP (P3.15) and one of the Class 1 OMPs (P1.16) when grown in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wall RA,Davies HA,Borriello SP

    更新日期:1989-11-01 00:00:00

  • Biotype and macromolecular profiles of cytotoxin-producing strains of Helicobacter pylori from antral gastric mucosa.

    abstract::Biotype, genome, protein and plasmid profile diversity amongst 40 epidemiologically unrelated strains of Helicobacter pylori was studied. Strains were API Zym biotypes II, III and IV but most (87%) were biotype II. Four subsets of strains were defined on a combination of motility (56% positive) and cytotoxin productio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Owen RJ,Bickley J,Moreno M,Costas M,Morgan DR

    更新日期:1991-04-15 00:00:00

  • Zinc content of the Bacillus anthracis lethal factor.

    abstract::We present evidence that the anthrax toxin lethal factor binds multiple zinc atoms. Results from atomic adsorption spectroscopy indicate that lethal factor contains approximately three zinc atoms per toxin molecule. Lethal factor treated with EDTA and o-phenanthroline contained a similar number of zinc atoms, indicati...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kochi SK,Schiavo G,Mock M,Montecucco C

    更新日期:1994-12-15 00:00:00

  • Genetic analysis of Carboxydothermus hydrogenoformans carbon monoxide dehydrogenase genes cooF and cooS.

    abstract::Carboxydothermus hydrogenoformans is an extremely thermophilic, Gram-positive bacterium growing on carbon monoxide (CO) as single carbon and energy source and producing only H(2) and CO(2). Carbon monoxide dehydrogenase is a key enzyme for CO metabolism. The carbon monoxide dehydrogenase genes cooF and cooS from C. hy...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: González JM,Robb FT

    更新日期:2000-10-15 00:00:00

  • Mycobacterium avium rough-to-smooth colony conversion resulting from growth in Tween 80 without presence of type-specific glycopeptidolipid antigens.

    abstract::Growth of a Mycobacterium avium complex serotype 20 rough-colony variant in 1.0% Tween 80 resulted in a smooth-colony morphological conversion that was reversible upon removal of Tween and was not associated with the presence of serotype-specific glycopeptidolipid antigens. Electron microscopic examination suggested t...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wright EL,Pourshafie M,Barrow WW

    更新日期:1992-11-01 00:00:00

  • Quantification of Agrobacterium tumefaciens C58 attachment to Arabidopsis thaliana roots.

    abstract::Agrobacterium tumefaciens is the causal agent of crown gall disease and is a vector for DNA transfer in transgenic plants. The transformation process by A. tumefaciens has been widely studied, but the attachment stage has not been well characterized. Most measurements of attachment have used microscopy and colony coun...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Petrovicheva A,Joyner J,Muth TR

    更新日期:2017-10-02 00:00:00

  • Accumulation of PHA granules in Cupriavidus necator as seen by confocal fluorescence microscopy.

    abstract::Many bacteria are capable of accumulating intracellular granules of polyhydroxyalkanoates (PHA). In this work, we developed confocal microscopy analysis of bacterial cells to study changes in the diameters of cells as well as PHA granules during growth and PHA accumulation in the bacterium Cupriavidus necator H16 (for...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mravec F,Obruca S,Krzyzanek V,Sedlacek P,Hrubanova K,Samek O,Kucera D,Benesova P,Nebesarova J

    更新日期:2016-05-01 00:00:00

  • Genetic variation among Mycoplasma agalactiae isolates detected by the variant surface lipoprotein gene (vspA) of Mycoplasma bovis.

    abstract::Multiple restriction fragments, homologous to the previously described Mycoplasma bovis vspA gene, were identified in the chromosome of Mycoplasma agalactiae. The vspA, a representative variable surface lipoprotein gene of the vsp gene family, and four synthetic oligonucleotides, representing sequences complementary t...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Flitman-Tene R,Levisohn S,Rosenbusch R,Rapoport E,Yogev D

    更新日期:1997-11-01 00:00:00

  • Phospholipid etherlipid and phospholipid fatty acid fingerprints in selected euryarchaeotal monocultures for taxonomic profiling.

    abstract::Phospholipid etherlipid (PLEL) derived isoprenoids and phospholipid fatty acids (PLFA) were determined in eight Euryarchaeotal monocultures for taxonomic profiling. For the first time significant amounts of fatty acids in the PLFA of Euryarchaeota were determined. The PLFA proportion varied between 11.3 and 35.5% of t...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gattinger A,Schloter M,Munch JC

    更新日期:2002-07-16 00:00:00

  • Cross-utilization and expression of outer membrane receptor proteins for siderophore uptake by Diamondback moth Plutella xylostella (Lepidoptera: Plutellidae) gut bacteria.

    abstract::Siderophore production by entomo- and phytopathogens, plus the cross-utilization of these siderophores and expression of outer membrane receptor proteins (OMRPs) by Diamondback moth (DBM) gut bacterial strains, were all examined. All the tested strains grew in the presence of 2, 2'-dipyridyl, and the Brachybacterium s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Indiragandhi P,Anandham R,Madhaiyan M,Kim GH,Sa T

    更新日期:2008-12-01 00:00:00

  • Biosynthesis of poly(4-hydroxybutyric acid) by recombinant strains of Escherichia coli.

    abstract::The aim of this study was the production of the homopolyester poly(4-hydroxybutyric acid) (poly(4HB)) with recombinant strains of Escherichia coli. Wild-type strains and other widely used non-recombinant strains of E. coli are not able to produce polyhydroxyalkanoic acids (PHA) as storage compounds and cannot utilize ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hein S,Söhling B,Gottschalk G,Steinbüchel A

    更新日期:1997-08-15 00:00:00

  • Characterization of a periplasmic peptidyl-prolyl cis-trans isomerase in Erwinia chrysanthemi.

    abstract::The main determinant of the plant pathogen Erwinia chrysanthemi virulence is the production of extracellular enzymes, mainly pectate lyases. Adjacent to a pectate lyase encoding locus, we identified the gene rotA supposed to encode a folding catalyst. Overproduction of the protein and assay of activity using a synthet...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Pissavin C,Hugouvieux-Cotte-Pattat N

    更新日期:1997-12-01 00:00:00

  • Physical and genetic map of the Listeria monocytogenes EGD serotype 1/2a chromosome.

    abstract::Listeria monocytogenes is a facultative intracellular pathogen responsible for both invasive and non-invasive food-borne illness in animals and humans. In this study, macrorestriction analysis following pulsed-field gel electrophoresis was used to show that Listeria monocytogenes serovar 1/2a strain EGD has a single c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: von Both U,Otten S,Darbouche A,Domann E,Chakraborty T

    更新日期:1999-06-15 00:00:00

  • Analysis of low-molecular mass products from biosolubilized coal.

    abstract::A relatively simple, rapid sample preparation method has been developed for analysis of low-molecular mass compounds present in soluble coal products generated by microbial coal solubilizing agents. Acidification of the sample followed by direct extraction into hexanes is coupled with gas chromatography/mass spectrome...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Toth-Allen J,Torzilli AP,Isbister JD

    更新日期:1994-03-01 00:00:00

  • Identification and characterization of Bacillus anthracis by multiplex PCR analysis of sequences on plasmids pXO1 and pXO2 and chromosomal DNA.

    abstract::Bacillus anthracis can be identified on the basis of the detection of virulence factor genes located on two plasmids, pXO1 and pXO2. Thus isolates lacking both pXO1 and pXO2 are indistinguishable from closely related B. cereus group bacteria. We developed a multiplex PCR assay for characterization of B. anthracis isol...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ramisse V,Patra G,Garrigue H,Guesdon JL,Mock M

    更新日期:1996-11-15 00:00:00

  • Purification and analysis of the antigenicity of a 69,000 Da protein from Bordetella pertussis.

    abstract::A purification scheme was devised for a 69-kDa outer membrane protein of Bordetella pertussis, a virulence-associated protein which may play a role in the pathogenesis of the organism. The protein was purified to apparent homogeneity by heating B. pertussis cells for 1 h at 60 degrees C followed by DEAE-Sepharose and ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gould-Kostka JL,Burns DL,Brennan MJ,Manclark CR

    更新日期:1990-02-01 00:00:00

  • DNA probes for identification of the ectomycorrhizal fungus Tuber magnatum Pico.

    abstract::The random amplified polymorphic DNA (RAPD) technique was used to develop DNA probes for the identification of ectomycorrhizal fungi belonging to the genus Tuber. RAPD fingerprinting revealed a high degree of interspecific variability and a low degree of intraspecific variability. One band (approximately 1.5 kb), cons...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lanfranco L,Wyss P,Marzachí C,Bonfante P

    更新日期:1993-12-15 00:00:00

  • Expression of the short fimbriae of Porphyromonas gingivalis is regulated in oral bacterial consortia.

    abstract::The Mfa1 protein of Porphyromonas gingivalis is the structural subunit of the short fimbriae and mediates coadhesion between P. gingivalis and Streptococcus gordonii. We utilized a promoter-lacZ reporter construct to examine the regulation of mfa1 expression in consortia with common oral plaque bacteria. Promoter acti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Park Y,James CE,Yoshimura F,Lamont RJ

    更新日期:2006-09-01 00:00:00

  • Direct profiling of the yeast dynamics in wine fermentations.

    abstract::We present a method to directly characterize the yeast diversity present in wine fermentations by employing denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 26S ribosomal RNA (rRNA) genes. PCR-DGGE of a portion of the 26S rRNA gene was shown to distinguish most yeast genera a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cocolin L,Bisson LF,Mills DA

    更新日期:2000-08-01 00:00:00

  • Cloning and characterization of the gene for the metalloprotease enterotoxin of Bacteroides fragilis.

    abstract::The Bacteroides fragilis enterotoxin is an extracellular zinc metalloprotease that has been implicated in diarrheal disease of humans and animals. This toxin causes fluid accumulation in intestinal loops and is cytotoxic for HT-29 cells, an intestinal carcinoma cell line. Here we report the cloning and sequencing of t...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kling JJ,Wright RL,Moncrief JS,Wilkins TD

    更新日期:1997-01-15 00:00:00

  • The immunosuppressive drug leflunomide affects mating-pheromone response and sporulation by different mechanisms in Saccharomyces cerevisiae.

    abstract::Leflunomide (LFM) is a novel anti-inflammatory and immunosuppressive drug, and inhibits the growth of cytokine-stimulated lymphoid cells in vitro. The effect of LFM on haploid and diploid cells of Saccharomyces cerevisiae was investigated to elucidate the molecular mechanism of action of the drug. Using a halo assay, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fujimura H

    更新日期:2000-10-01 00:00:00

  • Rapid identification of marine bioluminescent bacteria by amplified 16S ribosomal RNA gene restriction analysis.

    abstract::To rapidly identify natural isolates of marine bioluminescent bacteria, we developed amplified ribosomal DNA restriction analysis (ARDRA) methods. ARDRA, which is based on the restriction patterns of 16S rRNA gene digested with five enzymes (EcoRI, DdeI, HhaI, HinfI, RsaI), clearly distinguished the 14 species of mari...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kita-Tsukamoto K,Wada M,Yao K,Kamiya A,Yoshizawa S,Uchiyama N,Kogure K

    更新日期:2006-03-01 00:00:00

  • Gloeosporiocide, a new antifungal cyclic peptide from Streptomyces morookaense AM25 isolated from the Amazon bulk soil.

    abstract::Actinobacteria are known by their ability to produce several antimicrobial compounds of biotechnological interest. Thus, in this study, we isolated and identified by partial 16S RNA sequencing ∼100 actinobacteria isolates from guarana (Paullinia cupana) bulk soil. Besides, we isolated from the actinobacteria Streptomy...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Vicente Dos Reis G,Abraham WR,Grigoletto DF,de Campos JB,Marcon J,da Silva JA,Quecine MC,de Azevedo JL,Ferreira AG,de Lira SP

    更新日期:2019-07-01 00:00:00