Abstract:
:Multiple restriction fragments, homologous to the previously described Mycoplasma bovis vspA gene, were identified in the chromosome of Mycoplasma agalactiae. The vspA, a representative variable surface lipoprotein gene of the vsp gene family, and four synthetic oligonucleotides, representing sequences complementary to selected regions of the vsp genes, were used as probes against digested chromosomal DNAs of several M. agalactiae clinical isolates. The resulting Southern blot analysis demonstrated a marked DNA polymorphism of multiple vspA-related fragments among the isolates. An oligonucleotide representing a conserved 5'-region common to all known vsp genes, was found to hybridize to multiple M. agalactiae genomic fragments while the other three oligonucleotides, representing distinct repetitive structures within the coding region of three known vsp genes (vspA, vspE, and vspF), failed to react. These results argue for the possible existence of a gene family in M. agalactiae analogous to the vsp system of M. bovis but comprised of diverse genes.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Flitman-Tene R,Levisohn S,Rosenbusch R,Rapoport E,Yogev Ddoi
10.1111/j.1574-6968.1997.tb12716.xsubject
Has Abstractpub_date
1997-11-01 00:00:00pages
123-8issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(97)00413-8journal_volume
156pub_type
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