Abstract:
:The intracellular parasite Toxoplasma gondii is known to inhibit apoptosis of its host cell. The molecular mechanisms of this interference are, however, not yet completely understood. We show here that viable parasites prominently inhibited the activation of caspase 3/7 induced by cytochrome c, dATP and dithiothreitol in cytosolic extracts of human-derived Jurkat leukemic T cells. In contrast, granzyme B-induced caspase activity was only slightly diminished. De novo protein biosynthesis by T. gondii was dispensable for the inhibition of cytochrome c-induced caspase activation. Furthermore, a complete parasite lysate or, more importantly, molecules released by extracellular parasites mediated the interaction with the caspase cascade. The cell-free system applied here is thus a valuable tool to study the interaction of T. gondii and possibly other intracellular pathogens with host cell apoptosis.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Keller P,Schaumburg F,Fischer SF,Häcker G,Gross U,Lüder CGdoi
10.1111/j.1574-6968.2006.00241.xsubject
Has Abstractpub_date
2006-05-01 00:00:00pages
312-9issue
2eissn
0378-1097issn
1574-6968pii
FML241journal_volume
258pub_type
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