Abstract:
:The mechanism of adaptation of bacteria to survive at elevated temperature in the human host and the expression of heat-shock proteins in response to stress was examined by labelling with [35S]methionine. An increase in culture temperature from 26 degrees C to 37 degrees C induced expression of certain bacterial proteins (70 and 60 kDa). Heat shock at 40 degrees C, cold shock (10 degrees C), ethanol treatment or arsenite treatment also led to an increased expression of heat shock proteins of 70 and 60 kDa. Actinomycin D completely blocked the induction, indicating that transcription is required for the overexpression of stress proteins in Leuconostoc mesenteroides. N-terminal sequence analysis showed that these proteins were homologous to the highly conserved chaperone proteins DnaK and GroEL of Escherichia coli, respectively.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Salotra P,Singh DK,Seal KP,Krishna N,Jaffe H,Bhatnagar Rdoi
10.1111/j.1574-6968.1995.tb07754.xsubject
Has Abstractpub_date
1995-08-15 00:00:00pages
57-62issue
1eissn
0378-1097issn
1574-6968pii
037810979500235Wjournal_volume
131pub_type
杂志文章abstract::We developed a multiplex PCR to detect the presence of methicillin- (mecA), cadmium/zinc-(czrC) and antiseptic-resistant (qacA/B) staphylococci and to identify Panton-Valentine leukocidin (PVL)-positive and -negative Staphylococcus aureus and coagulase-negative staphylococci (CoNS) from infected and healthy eyes. The ...
journal_title:FEMS microbiology letters
pub_type: 信件
doi:10.1111/1574-6968.12383
更新日期:2014-03-01 00:00:00
abstract::The genes mphA and mphB encode macrolide 2'-phosphotransferases I and II, respectively, and they confer resistance to macrolide antibiotics in Escherichia coli. To study the expression of these genes in Gram-positive bacteria, we constructed recombinant plasmids that consisted of an mph gene and the pUB110 vector in B...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1998.tb12880.x
更新日期:1998-02-15 00:00:00
abstract::Helicobacter pullorum and Campylobacter lari are rarely isolated from humans with acute enteritis. Hitherto the two species could only be identified by genotypic techniques. Gas liquid chromatography of whole cell fatty acid extracts is described as the first phenotypic method for discrimination of the two species. Ch...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1998.tb13275.x
更新日期:1998-11-15 00:00:00
abstract::Induction, energy gain, effect on growth, and interaction of nitrate and nitrite reduction of Bradyrhizobium sp. (Lupinus) USDA 3045 were characterized. Both nitrate and nitrite were reduced in air, although nitrite reduction was insensitive to ammonium inhibition. Anaerobic reduction of both ions was shown to be link...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/S0378-1097(03)00620-7
更新日期:2003-09-26 00:00:00
abstract::Streptomyces netropsis SD-07, the producer of novel polyene macrolide antifungal antibiotics, was isolated from soil. For the investigation of the functions of its biosynthesis genes and regulation mechanisms, a genetic operating system is necessary. In this study, we successfully transferred the plasmid DNA of pSET15...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/1574-6968.12507
更新日期:2014-08-01 00:00:00
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pub_type: 杂志文章
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/0378-1097(89)90232-2
更新日期:1989-12-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:2017-04-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章,评审
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更新日期:2004-03-12 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2002.tb11322.x
更新日期:2002-08-27 00:00:00
abstract::Rhodococcus rhodochrous NCIMB13064 can dehalogenate and utilise a number of halogenated aliphatic compounds as sole carbon and energy source. Mutants of NCIMB13064 can be easily isolated with an enlarged range of 1-chloroalkane utilising ability. Dehalogenation of 1-chlorononane, 1-chlorodecane and short-chain 1-chlor...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1996.tb08582.x
更新日期:1996-12-01 00:00:00
abstract::Ninety-three Salmonella isolates recovered from commercial foods and exotic animals in Colombia were studied. The serotypes, resistance profiles and where applicable the quinolone resistance genes were determined. Salmonella Anatum (n=14), Uganda (19), Braenderup (10) and Newport (10) were the most prevalent serovars,...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:2010-12-01 00:00:00
abstract::A novel Toxoplasma gondii interspersed repeat element (TgIRE), present in most of the tachyzoite chromosomes, was characterised. Two regions on the TgIRE sequence showed high identity to two different T. gondii expressed sequence tag cDNAs of unknown function, which seems to be TgIRE pseudogenes. Two set of primers we...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2000.tb08984.x
更新日期:2000-03-01 00:00:00
abstract::Mycobacteria belonging to the Mycobacterium tuberculosis complex have the ability to invade and replicate in non-phagocytic cells, an event that requires the presence of bacterial surface components capable of triggering a cell response and the subsequent internalization of the microorganism. In this study, we report ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1999.tb13738.x
更新日期:1999-08-15 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6968.2008.01480.x
更新日期:2009-04-01 00:00:00
abstract::The clonal relationship of thirty E. coli strains of 0 antigen serotype 06 isolated from human, dog, pig or cow infections were investigated. Two main clones with serotypes 06 : H1 or 06 : H31, H- were identified. Isolates from humans, dogs, pigs and cows were found in both clones, indicating that animals are a possib...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/0378-1097(91)90600-f
更新日期:1991-05-15 00:00:00
abstract::Two plasmids, pEM4 (IncFI) determining virulence and pEM6 (IncI) determining colicine I production and resistance to tetracycline, have been found in a pathogenic strain of Citrobacter freundii. Even though pEM4 and pEM6 plasmids are conjugative and transfer themselves very efficiently during conjugation, a high cotra...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/0378-1097(90)90115-7
更新日期:1990-03-01 00:00:00
abstract::FlgM inhibits the flagella-specific sigma factor FliA and is involved in the mouse-virulence of Salmonella typhimurium. In recent experiments, we observed that: (i) a flgM gene that could function to negatively regulate flagella synthesis was present in a variety of salmonellae; and (ii) the flgM gene derived from Sal...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1996.tb08002.x
更新日期:1996-01-15 00:00:00
abstract::Oidium sp. has been recovered as an endophyte in Terminalia catappa (tropical chestnut) in Costa Rica. The volatile organic compounds (VOCs) of this organism uniquely and primarily consist of esters of propanoic acid, 2-methyl-, butanoic acid, 2-methyl-, and butanoic acid, 3-methyl-. The VOCs of Oidium sp. are slightl...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2008.01137.x
更新日期:2008-06-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:1996-10-01 00:00:00
abstract::This report describes Vibrio seventh pandemic island II (VSP-II) and three novel variants revealed by comparative genomics of 23 Vibrio cholerae strains and their presence among a large and diverse collection of V. cholerae isolates. Three VSP-II variants were reported previously and our results demonstrate the presen...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2010.02008.x
更新日期:2010-07-01 00:00:00
abstract::Salmonella Typhimurium harbors two Salmonella pathogenicity islands (SPIs), each encoding a type three secretion system for virulence proteins. Although there is increasing evidence of postinvasion roles for SPI-1, it has been generally accepted that SPI-1 genes are downregulated following the invasion process. Here, ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2011.02217.x
更新日期:2011-04-01 00:00:00
abstract::An 18-kDa protein that occurs in Vibrio cholerae has been described as an in vivo and low-iron regulated outer membrane antigen. Monoclonal antibodies which recognized this antigen were protective as passive vaccines in the infant rabbit model of cholera disease. In this study, those monoclonal antibodies were used in...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1993.tb06360.x
更新日期:1993-07-15 00:00:00
abstract::We report a rapid and reliable two-step multiplex polymerase chain reaction (PCR) assay to identify the 10 Bacteroides fragilis group species - Bacteroides caccae, B. distasonis, B. eggerthii, B. fragilis, B. merdae, B. ovatus, B. stercoris, B. thetaiotaomicron, B. uniformis and B. vulgatus. These 10 species were firs...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/S0378-1097(03)00296-9
更新日期:2003-05-16 00:00:00
abstract::Three (0140J, C197C and EF20) out of four strains of Streptococcus uberis exhibited high levels of bound plasmin activity following growth in the presence of bovine plasminogen. The remaining strain (C197) bound considerably less plasmin following growth in the same medium. In contrast to the others, this strain was u...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1997.tb12633.x
更新日期:1997-09-01 00:00:00
abstract::Growth of a Mycobacterium avium complex serotype 20 rough-colony variant in 1.0% Tween 80 resulted in a smooth-colony morphological conversion that was reversible upon removal of Tween and was not associated with the presence of serotype-specific glycopeptidolipid antigens. Electron microscopic examination suggested t...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/0378-1097(92)90158-k
更新日期:1992-11-01 00:00:00
abstract::Streptococcus pneumoniae strain JNR.7/87 is a highly virulent, type 4 encapsulated Gram-positive bacterium whose transformability has not been tested previously, and whose genome is currently being sequenced. The strain was transformed at very low efficiency by addition of exogenous competence-stimulating peptide: How...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1999.tb13460.x
更新日期:1999-03-15 00:00:00
abstract::Coagulase-negative staphylococci (CoNS) are common bacterial colonizers of the human skin. They are often involved in nosocomial infections due to biofilm formation in indwelling medical devices. While biofilm formation has been extensively studied in Staphylococcus epidermidis, little is known regarding other CoNS sp...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1093/femsle/fnv175
更新日期:2015-10-01 00:00:00
abstract::A cloning cassette, carrying a modified amino-glycoside resistance gene (neo) from transposon Tn5 was constructed. Three restriction sites internal to the neo gene were eliminated by in vitro mutagenesis, allowing their use in designing new cloning vectors. The original, suboptimal transcription promoter was replaced ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/0378-1097(91)90224-x
更新日期:1991-07-01 00:00:00
abstract::Antibiotic susceptibility, plasmid profiles and random amplification of polymorphic DNA (RAPD) were used to study strains of Vibrio vulnificus isolated from cockles (Anadara granosa). Thirty-six isolates were analyzed. The prevalent biotypes were 1 (72.2% of the isolates) and 2 (27.8%). Among these, 21 strains of biot...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1998.tb13138.x
更新日期:1998-08-01 00:00:00