SopB effector protein of Salmonella Typhimurium is translocated in mesenteric lymph nodes during murine salmonellosis.

abstract：:Currently, interest in phage science is on the rise again as this subject remains largely unexplored, and its potential diversity mainly untapped. Here, we present phage vB_BsuP-Goe1, a new isolate that infects Bacillus subtilis The phage forms round plaques with a matt outline on agar plates containing a B. subtilis ...

journal_title：FEMS microbiology letters

authors： Willms IM,Hertel R

更新日期：2016-10-01 00:00:00

abstract：:The detection of Trypanosoma cruzi kinetoplast DNA by polymerase chain reaction (PCR) amplification is a potentially powerful tool for the parasitological diagnosis of Chagas' disease. We have applied this technique in a field situation in Bolivia, where 45 children from a primary school were subjected to serological ...

journal_title：FEMS microbiology letters

authors： Wincker P,Bosseno MF,Britto C,Yaksic N,Cardoso MA,Morel CM,Brenière SF

更新日期：1994-12-15 00:00:00

abstract：:Two actinomycete strains, CHR3 and CHR28, were isolated from metal-contaminated sediments from Baltimore Inner Harbor. The isolates were classified as Streptomyces spp. Agar diffusion assays showed both isolates to be resistant to mercuric chloride and phenylmercuric acetate. Hybridization experiments indicated that g...

journal_title：FEMS microbiology letters

authors： Ravel J,Amoroso MJ,Colwell RR,Hill RT

更新日期：1998-05-01 00:00:00

abstract：:A structural characterization of polysaccharides extracted from the aposymbiotically cultured photobiont of the lichen Ramalina gracilis was carried out in order to compare them with those previously found in the symbiotic thallus. The photobiont was isolated from thallus fragments, following the method of Yamamoto, a...

journal_title：FEMS microbiology letters

authors： Cordeiro LM,Carbonero ER,Sassaki GL,Reis RA,Stocker-Wörgötter E,Gorin PA,Iacomini M

更新日期：2005-03-01 00:00:00

abstract：:While there is an extensive database of genes encoding ammonia monooxygenase (amo) found in the ammonia-oxidizing beta-proteobacteria, few amo sequences are available representing the gamma-proteobacteria. We sequenced the complete amo operon (amoCAB) for Nitrosococcus oceani (ATCC 19707), a marine, autotrophic, ammon...

journal_title：FEMS microbiology letters

authors： Alzerreca JJ,Norton JM,Klotz MG

更新日期：1999-11-01 00:00:00

abstract：:Strains of the filamentous gliding bacterium Vitreoscilla, LB13 and C1, are shown to be highly sensitive to UV-A (320-400 nm), with an LD50 of less than 20 kJ m(-2). Vitreoscilla LB13 can be protected from UV-A by including superoxide dismutase and catalase, separately or in combination, during the exposure, indicatin...

journal_title：FEMS microbiology letters

authors： Biggs LF,Moody AJ

更新日期：2003-07-15 00:00:00

abstract：:In the transition to the post-petroleum economy, there is a growing demand for novel enzymes with high process performances to replace traditional chemistry with a more 'green' approach. To date, microorganisms encompass the richest source of industrial biocatalysts, but the Earth-living microbiota remains largely unt...

journal_title：FEMS microbiology letters

authors： Berini F,Casciello C,Marcone GL,Marinelli F

更新日期：2017-11-15 00:00:00

abstract：:The nitrogen-fixing cyanobacterium Nostoc sp. ATCC 29132 was shown to contain two sequence-specific endonucleases. Nsp(29132) I was an isoschizomer of AsuII, and Nsp(29132) II was an isoschizomer of BamHI. Nsp(29132) II was shown to generate ends that could be ligated to those generated by BamHI. ...

journal_title：FEMS microbiology letters

authors： Muro-Pastor AM,Herrero A,Flores E

更新日期：1991-01-01 00:00:00

abstract：:There are no published methods on plasmid isolation from Peptostreptococcus spp., therefore two methods of plasmid isolation from this genera were analysed: the boiling and alkaline-SDS methods. Plasmid DNA was not recovered by the boiling method, however, with the alkaline-SDS method, cryptic plasmid DNA was detected...

journal_title：FEMS microbiology letters

authors： Ng LK,Dillon JA

更新日期：1989-10-01 00:00:00

abstract：:Different genes encoding pyridine nucleotide transhydrogenases (pntAB, udhA) and acetoacetyl-CoA reductases (phaB) were heterologously overexpressed in Rhodospirillum rubrum S1. A recombinant strain, which harbored the gene encoding the membrane-bound transhydrogenase PntAB from Escherichia coli MG1655 and the phaB1 g...

journal_title：FEMS microbiology letters

authors： Heinrich D,Raberg M,Steinbüchel A

更新日期：2015-04-01 00:00:00

abstract：:High quality Fourier transform infrared (FTIR) spectra were acquired from living Micrasterias hardyi cells maintained in an IR transparent flow-through cell using a FTIR microscope coupled to a synchrotron light source. Spectral maps of living, nutrient-replete cells showed band intensities consistent with the known l...

journal_title：FEMS microbiology letters

authors： Heraud P,Wood BR,Tobin MJ,Beardall J,McNaughton D

更新日期：2005-08-15 00:00:00

abstract：:A Gram-negative sporulating thermophilic anaerobe, designated AB11Ad, was isolated from the heated waters of the Great Artesian Basin of Australia. It grew on a variety of carbohydrates including glucose, starch, and dextran and produced a thermostable and thermoactive extracellular endo-dextranase. The enzyme was pro...

journal_title：FEMS microbiology letters

authors： Wynter C,Patel BK,Bain P,de Jersey J,Hamilton S,Inkerman PA

更新日期：1996-07-01 00:00:00

abstract：:Clostridium difficile and Clostridium perfringens type A are infectious agents of enteric diseases. The main virulence factors of these microorganisms include toxins A and B of C. difficile (ToxA and ToxB) and enterotoxin of C. perfringens (Cpe). In this study genetic constructions have been created for the expression...

journal_title：FEMS microbiology letters

authors： Belyi IF,Varfolomeeva NA

更新日期：2003-08-29 00:00:00

abstract：:The degradation of a series of nitroaromatic compounds by the lignin-degrading fungus Phanerochaete chrysosporium was examined. From 4-nitrotoluene (4-NT), several metabolic intermediates were identified. Initially, 4-NT was converted to 4-nitrobenzyl alcohol (4-NBA), followed by the oxidation reactions to form 4-nitr...

journal_title：FEMS microbiology letters

authors： Teramoto H,Tanaka H,Wariishi H

更新日期：2004-05-15 00:00:00

abstract：:This review is devoted to the mechanisms of antibiotic resistance in mollicutes (class Bacilli, subclass Mollicutes), the smallest self-replicating bacteria, that can cause diseases in plants, animals and humans, and also contaminate cell cultures and vaccine preparations. Research in this area has been mainly based o...

journal_title：FEMS microbiology letters

authors： Chernov VM,Chernova OA,Mouzykantov AA,Medvedeva ES,Baranova NB,Malygina TY,Aminov RI,Trushin MV

更新日期：2018-09-01 00:00:00

abstract：:The interaction of Entamoeba histolytica trophozoites with collagen type I and calcium induces a membrane to nuclei signaling. The transduction pathways involved in such phenomena are still poorly understood. Using a combination of immunoprecipitation assays, Western immunoblot analysis, electrophoretic mobility shift...

journal_title：FEMS microbiology letters

authors： Cruz-Vera J,Clara L,Hernández-Kelly R,Alfredo Méndez J,Pérez-Salazar E,Ortega A

更新日期：2003-12-12 00:00:00

abstract：:The biosynthesis of cephalosporins involving a thiozolidine ring expansion is catalyzed by deacetoxycephalosporin C synthase (DAOCS). In this study, three DAOCS isozymes were cloned and expressed as active enzymes together with Streptomyces jumonjinensis DAOCS that was newly isolated and partially characterized. The e...

journal_title：FEMS microbiology letters

authors： Chin HS,Sim J,Seah KI,Sim TS

更新日期：2003-01-28 00:00:00

abstract：:The central carbon metabolism is well investigated in bacteria, but this is not the case for archaea. MJ0400-His(6) from Methanocaldococcus jannaschii catalyzes the cleavage of fructose-1,6-bisphosphate (FBP) to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate with a V(max) of 33 mU mg(-1) and a K(m) of 430 m...

journal_title：FEMS microbiology letters

authors： Samland AK,Wang M,Sprenger GA

更新日期：2008-04-01 00:00:00

abstract：:Vibrio cholerae non-O1 strain N037 produced a hemolysin (NO37-Hly) which was antigenically similar to E1 Tor hemolysin (E1 Tor-Hly) but different in molecular size, hemolytic activity, and glucose binding capacity. In the gene encoding NO37-Hly, a 4-bp insertion into the structural gene for E1 Tor-Hly (hlyA) was found...

journal_title：FEMS microbiology letters

authors： Honma Y,Yamamoto K,Iwanaga M

更新日期：1995-11-01 00:00:00

abstract：:The aim of this study was to evaluate the ability of a nested PCR system to detect Salmonella senftenberg in raw oysters. The specific primers of the PCR were derived from the invA gene sequence, essential for Salmonella invasiveness into epithelial cells. First, for the extraction of DNA, four methods (guanidine isot...

journal_title：FEMS microbiology letters

authors： Vázquez-Novelle MD,Pazos AJ,Abad M,Sánchez JL,Pérez-Parallé ML

更新日期：2005-02-01 00:00:00

abstract：:Ammonium transporters form a conserved family of transport proteins and are widely distributed among all domains of life. The genome of Nitrosomonas europaea codes for a single gene (rh1) that belongs to the family of the AMT/Rh ammonium transporters. For the first time, this study provides functional and physiologica...

journal_title：FEMS microbiology letters

authors： Weidinger K,Neuhäuser B,Gilch S,Ludewig U,Meyer O,Schmidt I

更新日期：2007-08-01 00:00:00

abstract：:Actinobacteria are known by their ability to produce several antimicrobial compounds of biotechnological interest. Thus, in this study, we isolated and identified by partial 16S RNA sequencing ∼100 actinobacteria isolates from guarana (Paullinia cupana) bulk soil. Besides, we isolated from the actinobacteria Streptomy...

journal_title：FEMS microbiology letters

authors： Vicente Dos Reis G,Abraham WR,Grigoletto DF,de Campos JB,Marcon J,da Silva JA,Quecine MC,de Azevedo JL,Ferreira AG,de Lira SP

更新日期：2019-07-01 00:00:00

abstract：:The genome size of Actinobacillus pleuropneumoniae was determined by pulsed field gel electrophoresis of AscI and ApaI digested chromosomal DNA. The genome size of the type strain 4074T (serotype 1) was determined to be 2404 +/- 40 kb. The chromosome sizes for the reference strains of the other serotypes range between...

journal_title：FEMS microbiology letters

authors： Chevallier B,Dugourd D,Tarasiuk K,Harel J,Gottschalk M,Kobisch M,Frey J

更新日期：1998-03-15 00:00:00

abstract：:The viability of probiotics is affected by several factors during manufacturing, storage and gastrointestinal tract passage. Protecting the probiotics from harmful conditions is particularly critical for oxygen sensitive species like Akkermansia muciniphila, a bacterium which recently has been proposed as a next-gener...

journal_title：FEMS microbiology letters

authors： Marcial-Coba MS,Saaby L,Knøchel S,Nielsen DS

更新日期：2019-01-01 00:00:00

abstract：:We have cloned and sequenced the rfaH gene from Salmonella enterica serovar Typhi strain Ty2. The gene showed a high degree of similarity to the rfaH genes from Escherichia coli K-12 and S. enterica serovar Typhimurium. A rfaH mutant was constructed by site-directed mutagenesis. This mutant produced a rough lipopolysa...

journal_title：FEMS microbiology letters

authors： Rojas G,Saldías S,Bittner M,Zaldívar M,Contreras I

更新日期：2001-10-16 00:00:00

abstract：:The signal peptidase (SPase) SipS of Bacillus subtilis is responsible for the processing of precursors of secreted proteins. It differs from the SPases of Gram-negative bacteria in structure and specificity. To assay the activity of SipS in vitro, two efficient transcription-translation systems for the synthesis of ra...

journal_title：FEMS microbiology letters

authors： Vehmaanperä J,Görner A,Venema G,Bron S,van Dijl JM

更新日期：1993-12-01 00:00:00

abstract：:An isolation procedure was developed to provide within one day microcystin-LR, a cyclic heptapeptide toxin from Microcystis aeruginosa PCC 7806. After ODS (octadecylsilyl) solid phase extraction, the crude toxin fraction was chromatographed using a strong anion exchange column. The toxin was eluted with 0.02 M ammoniu...

journal_title：FEMS microbiology letters

authors： Martin C,Sivonen K,Matern U,Dierstein R,Weckesser J

更新日期：1990-03-01 00:00:00

abstract：:The ascomycete Sclerotinia sclerotiorum is a plant pathogen with a very broad host range. In order to identify and characterize genes involved in S. sclerotiorum infection of Brassica napus (canola), expressed sequence tags (ESTs) were examined from libraries prepared from three tissues: complex appressorium (infectio...

journal_title：FEMS microbiology letters

authors： Sexton AC,Cozijnsen AJ,Keniry A,Jewell E,Love CG,Batley J,Edwards D,Howlett BJ

更新日期：2006-05-01 00:00:00

abstract：:A strain of Agromyces fucosus, designated Vs2, metabolized a range of organophosphonate compounds as sole phosphorus sources for growth and metabolized phosphonoacetate as a sole carbon, energy and phosphorus source for growth. With phosphonoacetate as the sole phosphorus source and a pyruvate carbon source, transient...

journal_title：FEMS microbiology letters

authors： O'Loughlin SN,Graham RL,McMullan G,Ternan NG

更新日期：2006-08-01 00:00:00

abstract：:The need for improved rapid diagnostic tests for tuberculosis disease has prompted interest in the volatile organic compounds (VOCs) emitted by Mycobacterium tuberculosis complex bacteria. We have investigated VOCs emitted by Mycobacterium bovis BCG grown on Lowenstein-Jensen media using selected ion flow tube mass sp...

journal_title：FEMS microbiology letters

authors： McNerney R,Mallard K,Okolo PI,Turner C

更新日期：2012-03-01 00:00:00