Screening and analysis of DNA fragments that show promoter activities in Thermus thermophilus.

abstract：:Streptococcus suis (S. suis) is an emerging zoonotic agent that exhibits high level resistance to classic antibiotics and a heavy burden in the swine industry. Therefore alternative antibacterial agents need to be developed. A novel endolysin derived from the S. suis temperate phage phi5218, termed Ply5218, was identi...

journal_title：FEMS microbiology letters

authors： Zhang H,Zhang C,Wang H,Yan YX,Sun J

更新日期：2016-09-01 00:00:00

abstract：:It is often claimed that Antoni van Leeuwenhoek was secretive about his methods. However, closer examination of his letters suggests that this reputation was not always deserved. Some letters were not published in the Royal Society Proceedings, and others were edited. This paper describes the repetition of his experim...

journal_title：FEMS microbiology letters

authors： Robertson LA

更新日期：2019-08-01 00:00:00

abstract：:Species of strictly and facultatively anaerobic cellulolytic bacteria from soils and sediments were examined for the ability to degrade chitin. Of 22 species studied, 16 degraded insoluble chitin. Cellulomonas uda, which was selected for a comparative study of its cellulase and chitinase enzyme systems, produced diffe...

journal_title：FEMS microbiology letters

authors： Reguera G,Leschine SB

更新日期：2001-11-13 00:00:00

abstract：:The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma 70-type promoter. This promoter responds to heat shock and involves an inverted repeat different from t...

journal_title：FEMS microbiology letters

authors： Brans A,Loriaux A,Thamm I,Joris B,Dusart J

更新日期：1997-04-15 00:00:00

abstract：:Microbial competition for carbon sources is a primary determinant of rhizosphere ecology. We employed the PCR to examine the population fluctuations of a symbiotic nitrogen-fixing bacterium (Rhizobium etli) during the first 11 days following inoculation of Phaseolus vulgaris seedlings grown in the presence or absence ...

journal_title：FEMS microbiology letters

authors： Petersen DJ,Srinivasan M,Chanway CP

更新日期：1996-09-01 00:00:00

abstract：:A number of enteric pathogens, including enteropathogenic (EPEC) and enterohemorrhagic (EHEC) Escherichia coli, Hafnia alvei, a strain of Citrobacter freundii, and rabbit EPEC strain RDEC-1 cause attaching-effacing (AE) lesions in the gut mucosa. These bacteria have a pathogenicity cassette (locus of enterocyte efface...

journal_title：FEMS microbiology letters

authors： Agin TS,Cantey JR,Boedeker EC,Wolf MK

更新日期：1996-11-01 00:00:00

abstract：:During programs for the selection of enological yeasts, several hundred natural isolates are usually screened. The scope of these operations is to isolate strains possessing good fermentative properties without necessarily arriving at a precise species designation: in other words, to detect strains belonging to the Sa...

journal_title：FEMS microbiology letters

authors： Nardi T,Carlot M,De Bortoli E,Corich V,Giacomini A

更新日期：2006-11-01 00:00:00

abstract：:The effectiveness of an educational board game developed to teach the pharmacology of antimicrobial drugs to medical students was compared with the lecture-based seminar as a supplemental tool to improve short- and long-term knowledge retention and the perception of the learning method by students. A group of 124 stud...

journal_title：FEMS microbiology letters

authors： Karbownik MS,Wiktorowska-Owczarek A,Kowalczyk E,Kwarta P,Mokros Ł,Pietras T

更新日期：2016-04-01 00:00:00

abstract：:The presence of insertion sequence IS1 in 70 multiple-antibiotic resistant clinical strains was determined. This 70-strain collection comprised 46 Escherichia coli, 18 Salmonella and 6 Shigella strains. The presence of IS1 was detected in the chromosome and plasmids of 73% and 63% of the strains, respectively, and 51%...

journal_title：FEMS microbiology letters

authors： Ramirez-Santos J,Alvarez G,Cisneros E,Gomez-Eichelmann MC

更新日期：1992-06-01 00:00:00

abstract：:We have reported that the macrophage-like cell line J774.1, when infected with the periodontopathic bacterium Actinobacillus actinomycetemcomitans, undergoes apoptosis. In this study, we examined whether stimulation of J774.1 cells with lipopolysaccharide (LPS) before the infection affects the subsequent apoptosis. Cy...

journal_title：FEMS microbiology letters

authors： Muro M,Nakashima K,Tomioka J,Kato S,Nonaka K,Yoshida T,Inoue M,Nishihara T,Kowashi Y

更新日期：1999-06-15 00:00:00

abstract：:A Deltalgt (Lgt, lipoprotein diacylglyceryl transferase) isogenic mutant was obtained which indicates that lgt is not essential for cell growth in vitro, like in the Gram-positive bacterium Bacillus subtilis, but unlike in the proteobacteria Escherichia coli and Salmonella typhimurium. The mutation was transduced to a...

journal_title：FEMS microbiology letters

authors： Petit CM,Brown JR,Ingraham K,Bryant AP,Holmes DJ

更新日期：2001-06-25 00:00:00

abstract：:Two conservative gene clusters, the S10 ribosomal protein region and one (of the two) set of rRNA genes, were split in a genome crossover rearrangement event in Mycoplasma gallisepticum. As a result of the rearrangement the major part of the S10 ribosomal protein cluster is located upstream of genes for 23S-5S rRNA (r...

journal_title：FEMS microbiology letters

authors： Skamrov A,Feoktistova E,Goldman M,Beabealashvilli R

更新日期：2002-03-05 00:00:00

abstract：:This study describes how bkaR, a highly conserved mycobacterial TetR-like transcriptional repressor, regulates a number of nearby genes that have associations with branched-chain keto-acid metabolism. bkaR (MSMEG_4718) was deleted from the nonpathogenic species Mycobacterium smegmatis, and changes in global gene expre...

journal_title：FEMS microbiology letters

authors： Balhana RJ,Swanston SN,Coade S,Withers M,Sikder MH,Stoker NG,Kendall SL

更新日期：2013-08-01 00:00:00

abstract：:We report a rapid and reliable two-step multiplex polymerase chain reaction (PCR) assay to identify the 10 Bacteroides fragilis group species - Bacteroides caccae, B. distasonis, B. eggerthii, B. fragilis, B. merdae, B. ovatus, B. stercoris, B. thetaiotaomicron, B. uniformis and B. vulgatus. These 10 species were firs...

journal_title：FEMS microbiology letters

authors： Liu C,Song Y,McTeague M,Vu AW,Wexler H,Finegold SM

更新日期：2003-05-16 00:00:00

abstract：:Helicobacter pylori possesses a gene (HP0326/JHP309) homologous to neuA of other bacteria, encoding a cytidyl monophosphate-N-acetylneuraminic acid synthetase-homologous enzyme in its N-terminal portion. We analysed the function of this gene, which is controlled by a flagellar class 2 sigma(54) promoter, in flagellar ...

journal_title：FEMS microbiology letters

authors： Josenhans C,Vossebein L,Friedrich S,Suerbaum S

更新日期：2002-05-07 00:00:00

abstract：:The transport of radioactively labelled uracil into submerged mycelium of T. viride was measured by means of a membrane filtration technique. It was found to be time-dependent (up to 90 min) and concentration-dependent (up to 8 mmol l-1). Its concentration dependence was biphasic and consisted from the saturatable par...

journal_title：FEMS microbiology letters

authors： Lakatos B,Simkovic M,Betina V,Varecka L

更新日期：1999-02-15 00:00:00

abstract：:Glutamate uptake in the Gram-positive Corynebacterium glutamicum is mediated via a binding protein-dependent transport system, which is encoded by the gluABCD gene cluster. Cloning of these genes in an expression vector and subsequent transformation of the resulting plasmid allows different strains of the Gram-negativ...

journal_title：FEMS microbiology letters

authors： Burkovski A,Krämer R

更新日期：1995-04-01 00:00:00

abstract：:Fusobacterium nucleatum is a common oral anaerobe associated with gingivitis, periodontal disease and preterm deliveries. Coaggregation among oral bacteria is considered to be a significant factor in dental plaque development. Adhesion to host cells was suggested to be important for the F. nucleatum virulence associat...

journal_title：FEMS microbiology letters

authors： Bachrach G,Ianculovici C,Naor R,Weiss EI

更新日期：2005-07-15 00:00:00

abstract：:Pseudomonas fragi is a meat and milk spoilage bacterium with high iron requirements; however, mechanisms of iron acquisition remain largely unknown. The aim of this work was to investigate siderophore production as an iron acquisition system for P. fragi. A vibrioferrin siderophore gene cluster was identified in 13 P....

journal_title：FEMS microbiology letters

authors： Stanborough T,Fegan N,Powell SM,Tamplin M,Chandry PS

更新日期：2018-03-01 00:00:00

abstract：:The denitrifying bacterium Thauera aromatica strain AR-1 grows anaerobically with protocatechuate (3,4-dihydroxybenzoate (DHB)) as sole energy and carbon source. This bacterium harbors two distinct pathways for degradation of aromatic compounds, the benzoyl-coenzyme A (CoA) pathway for benzoate degradation and the hyd...

journal_title：FEMS microbiology letters

authors： Philipp B,Kemmler D,Hellstern J,Gorny N,Caballero A,Schink B

更新日期：2002-06-18 00:00:00

abstract：:Among aerobic prokaryotes, many different terminal oxidase complexes have been described. Sequence comparison has revealed that the aa3-type cytochrome c oxidase and the bo3-type quinol oxidase are variations on the same theme: the heme-copper oxidase. A third member of this family has recently been recognized: the cb...

journal_title：FEMS microbiology letters

authors： van der Oost J,de Boer AP,de Gier JW,Zumft WG,Stouthamer AH,van Spanning RJ

更新日期：1994-08-01 00:00:00

abstract：:Desulfovibrio fructosovorans possesses two periplasmic hydrogenases (a nickel-iron and an iron hydrogenase) and a cytoplasmic NADP-dependent hydrogenase. The hydAB genes encoding the periplasmic iron hydrogenase were replaced, in the wild-type strain as well as in single mutants depleted of one of the other two hydrog...

journal_title：FEMS microbiology letters

authors： Casalot L,Valette O,De Luca G,Dermoun Z,Rousset M,de Philip P

更新日期：2002-08-27 00:00:00

abstract：:We have constructed a fungal subtractive suppressive library enriched in genes expressed during appressorium maturation in Magnaporthe grisea. Sequencing of 250 clones from the subtracted appressorium cDNA library revealed 142 unique genes, represented by 155 non-redundant ESTs (expressed sequence tags). Of these ESTs...

journal_title：FEMS microbiology letters

authors： Lu JP,Liu TB,Lin FC

更新日期：2005-04-01 00:00:00

abstract：:A molecular cloning strategy has been designed to isolate the gene that encodes the small cytoplasmic RNA (scRNA) component of bacterial signal recognition particles. Using this strategy a putative Listeria monocytogenes scRNA lambda gt11 recombinant clone was isolated. A previously described complementation assay dev...

journal_title：FEMS microbiology letters

authors： Barry T,Kelly M,Glynn B,Peden J

更新日期：1999-04-01 00:00:00

abstract：:Escherichia coli is the most common organism associated with asymptomatic bacteriuria (ABU) in humans. In contrast to uropathogenic E. coli (UPEC) that cause symptomatic urinary tract infection, very little is known about the mechanisms by which these strains colonize the urinary tract. Here, we have investigated the ...

journal_title：FEMS microbiology letters

authors： Hancock V,Ferrières L,Klemm P

更新日期：2007-02-01 00:00:00

abstract：:The beta-lactamase genes blaA and blaB were detected by PCR amplification in strains of Yersinia enterocolitica biovar 1A isolated from India, Germany, France and the USA. Both genes were detected in all strains. Polymerase chain reaction-restriction fragment length polymorphism revealed genetic heterogeneity in blaA ...

journal_title：FEMS microbiology letters

authors： Sharma S,Mittal S,Mallik S,Virdi JS

更新日期：2006-04-01 00:00:00

abstract：:Divergicin 750, a bacteriocin produced by Carnobacterium divergens 750, preferentially inhibited the growth of strains of Carnobacterium and Enterococcus. Selected strains of Listeria monocytogenes and Clostridium perfringens were also inhibited. The bacteriocin was purified to homogeneity by ammonium sulfate precipit...

journal_title：FEMS microbiology letters

authors： Holck A,Axelsson L,Schillinger U

更新日期：1996-02-15 00:00:00

abstract：:We have tested the expression of alkaline phosphatases in a mutant strain of Escherichia coli deficient in dipZ, a gene coding for a protein involved in cytochrome c biogenesis, and the isogenic wild-type strain. The yield of soluble and active human placental alkaline phosphatase was significantly reduced in the muta...

journal_title：FEMS microbiology letters

authors： Beck R,Crooke H,Jarsch M,Cole J,Burtscher H

更新日期：1994-12-01 00:00:00

abstract：:The natural life cycle of Vibrio cholerae involves the transitioning of cells between different environmental surfaces such as the chitinous shell of Crustaceae and the epithelial layer of the human intestine. Previous studies using static biofilm systems showed a strict dependence of biofilm formation on the vps and ...

journal_title：FEMS microbiology letters

authors： Müller J,Miller MC,Nielsen AT,Schoolnik GK,Spormann AM

更新日期：2007-10-01 00:00:00

abstract：:The xylanase gene of Streptomyces flavogriseus was cloned in pUC8 plasmid and expressed in Escherichia coli lysogenic for lambda cI857. lambda-Induced lysis of E. coli at 42 degrees C allowed efficient release of cloned enzyme activity in extracellular environment. The xylanase gene was located in the 0.8-kb HindIII f...

journal_title：FEMS microbiology letters

authors： Srivastava R,Ali SS,Srivastava BS

更新日期：1991-03-01 00:00:00