Molecular cloning and phylogenetic analysis of the small cytoplasmic RNA from Listeria monocytogenes.

Abstract:

:A molecular cloning strategy has been designed to isolate the gene that encodes the small cytoplasmic RNA (scRNA) component of bacterial signal recognition particles. Using this strategy a putative Listeria monocytogenes scRNA lambda gt11 recombinant clone was isolated. A previously described complementation assay developed to genetically select functional homologues of 4.5S RNA and scRNA of bacteria confirmed that the lambda gt11 recombinant clone isolated encoded for the scRNA from L. monocytogenes. A secondary structure for this scRNA is proposed and a phylogenetic comparison of the 276 base L. monocytogenes scRNA with previously characterised Gram-positive bacterial scRNAs is also presented.

journal_name

FEMS Microbiol Lett

authors

Barry T,Kelly M,Glynn B,Peden J

doi

10.1111/j.1574-6968.1999.tb13483.x

keywords:

subject

Has Abstract

pub_date

1999-04-01 00:00:00

pages

47-53

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(99)00055-5

journal_volume

173

pub_type

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