Abstract:
:A molecular cloning strategy has been designed to isolate the gene that encodes the small cytoplasmic RNA (scRNA) component of bacterial signal recognition particles. Using this strategy a putative Listeria monocytogenes scRNA lambda gt11 recombinant clone was isolated. A previously described complementation assay developed to genetically select functional homologues of 4.5S RNA and scRNA of bacteria confirmed that the lambda gt11 recombinant clone isolated encoded for the scRNA from L. monocytogenes. A secondary structure for this scRNA is proposed and a phylogenetic comparison of the 276 base L. monocytogenes scRNA with previously characterised Gram-positive bacterial scRNAs is also presented.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Barry T,Kelly M,Glynn B,Peden Jdoi
10.1111/j.1574-6968.1999.tb13483.xkeywords:
subject
Has Abstractpub_date
1999-04-01 00:00:00pages
47-53issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(99)00055-5journal_volume
173pub_type
杂志文章abstract::Group B streptococci (GBS) represent a very important group of human pathogens. So far little is known about the mechanisms by which these bacteria can cause disease and the bacterial factors involved. One putative virulence factor is the beta antigen of the C protein complex (Bac), which can bind to the Fc region of ...
journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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