A molecular marker diagnostic of a specific isolate of an arbuscular mycorrhizal fungus, Gigaspora margarita.

Abstract:

:To investigate the auto-ecology of a strain of Gigaspora margarita in a commercial inoculum, we found a pair of PCR primers amplifying a sequence of 235 bp diagnostic of the isolate. We designed an oligonucleotide probe based on the DNA sequence. The combination of PCR and the probing successfully detected the diagnostic sequence from both DNA preparations of single spores and colonized roots. This protocol enabled us to distinguish the isolate among several isolates from Japan, Nepal and the USA.

journal_name

FEMS Microbiol Lett

authors

Yokoyama K,Tateishi T,Marumoto T,Saito M

doi

10.1111/j.1574-6968.2002.tb11262.x

keywords:

subject

Has Abstract

pub_date

2002-07-02 00:00:00

pages

171-5

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378109702007140

journal_volume

212

pub_type

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