Inhibition of aerobic respiration and dissimilatory perchlorate reduction using cyanide.

abstract：:Levan fructotransferase (LFTase) from Arthrobacter ureafaciens K2032 was expressed with N-terminal fusion of a LacZ-derived secretion motif (TMITNSSSVP) using the lac promoter system in recombinant Escherichia coli JM109 [pUDF-A81]. In flask cultures, recombinant enzyme activity was detected in culture media, and sequ...

journal_title：FEMS microbiology letters

authors： Lee J,Saraswat V,Koh I,Song KB,Park YH,Rhee SK

更新日期：2001-02-20 00:00:00

abstract：:Recently, a novel verocytotoxin named VT2y was described which belongs to the STx family and is produced by Escherichia coli isolated from domestic poultry with swollen head syndrome (SHS). The VT2y toxin induced apoptosis in Vero, HeLa, CHO, CEF (primary chicken embryo fibroblast) and PCK (primary chicken kidney) cel...

journal_title：FEMS microbiology letters

authors： Salvadori MR,Yamada AT,Yano T

更新日期：2001-04-01 00:00:00

abstract：:In the current studies, we used Lineweaver-Burke analysis to examine the role of 1-hydroxybenzotriazole (HBT) in the oxidation of various compounds by laccase from Trametes versicolor. At low concentrations, HBT was a competitive inhibitor of the oxidation, but at high concentrations, it was a noncompetitive inhibitor...

journal_title：FEMS microbiology letters

authors： Hirai H,Shibata H,Kawai S,Nishida T

更新日期：2006-12-01 00:00:00

abstract：:A solvent-tolerant, slightly thermophilic bacterium was isolated at 45 degrees C in the presence of toluene vapor provided as the sole carbon source. Strain T27 was identified as Deinococcus geothermalis T27. It could tolerate high concentrations of solvent provided as a nonaqueous layer (5% and 20%, v/v) to a cell su...

journal_title：FEMS microbiology letters

authors： Kongpol A,Kato J,Vangnai AS

更新日期：2008-09-01 00:00:00

abstract：:This study provides a novel qRT-PCR protocol for specific detection and proof of viability of Phytophthora in environmental samples based on differential accumulation of cox II transcripts. Chemical and physical treatments were tested for their ability to induce in vitro the accumulation of cytochrome oxidase genes en...

journal_title：FEMS microbiology letters

authors： Vannini A,Tomassini A,Bruni N,Vettraino AM

更新日期：2014-04-01 00:00:00

abstract：:We analyzed gel electrophoretic banding patterns of penicillin-binding proteins (PBPs) of 16 type strains of coagulase-negative staphylococci (CNS). S. epidermidis, S. haemolyticus, S. saprophyticus, S. hominis, S. xylosus, S. simulans, S. warneri, S. capitis, S. saccharolyticus, S. auricularis, S. caseolyticus, S. ga...

journal_title：FEMS microbiology letters

authors： Kanda K,Hiramatsu K,Yokota T

更新日期：1990-10-01 00:00:00

abstract：:We developed a DNA microarray for identification of Bacillus anthracis and other phylogenetic groupings within the "Bacillus cereus group". Nucleotide sequences of 16S-23S ribosomal DNA internal transcribed spacers containing genes for tRNA(Ile) from 52 B. anthracis strains were found to be identical to sequences from...

journal_title：FEMS microbiology letters

authors： Nübel U,Schmidt PM,Reiss E,Bier F,Beyer W,Naumann D

更新日期：2004-11-15 00:00:00

abstract：:Streptococcus suis is a worldwide cause of various swine infections and is also an important agent of zoonosis. Strains of S. suis are classified according to their serotype, and currently, 35 serotypes are recognized. The aim of this study was to characterize nontypeable isolates of S. suis with regard to their cell ...

journal_title：FEMS microbiology letters

authors： Bonifait L,Gottschalk M,Grenier D

更新日期：2010-10-01 00:00:00

abstract：:Mycobacterium smegmatis was used to study the relationship between DNA repair processes involving RecA and nonhomologous end joining (NHEJ). The effect of gene deletions in recA and/or in two genes involved in NHEJ (ku and ligD) was tested on the ability of bacteria to join breaks in plasmids transformed into them and...

journal_title：FEMS microbiology letters

authors： Korycka-Machala M,Brzostek A,Rozalska S,Rumijowska-Galewicz A,Dziedzic R,Bowater R,Dziadek J

更新日期：2006-05-01 00:00:00

abstract：:To investigate the auto-ecology of a strain of Gigaspora margarita in a commercial inoculum, we found a pair of PCR primers amplifying a sequence of 235 bp diagnostic of the isolate. We designed an oligonucleotide probe based on the DNA sequence. The combination of PCR and the probing successfully detected the diagnos...

journal_title：FEMS microbiology letters

authors： Yokoyama K,Tateishi T,Marumoto T,Saito M

更新日期：2002-07-02 00:00:00

abstract：:Two variants have been isolated from the wild-type Azospirillum lipoferum strain 4B. The first variant, 4V(I), spontaneously emerged from the wild-type at frequencies in the order of 10(-4) to 10(-3) per cell generation. Compared to the wild-type, the 4V(I) variant gained (production of a carotenoid-like pigment, assi...

journal_title：FEMS microbiology letters

authors： Alexandre G,Bally R

更新日期：1999-05-15 00:00:00

abstract：:A broad range of different chemical and pharmaceutical compounds have been produced in microbial cell factories. To compete with traditional crude oil based production processes, the use of complex alternative raw materials such as lignocellulosic biomass, waste streams and utilization of CO2 in gas fermentations has ...

journal_title：FEMS microbiology letters

authors： Novak K,Pflügl S

更新日期：2018-10-01 00:00:00

abstract：:Bacteriocins from gram-positive bacteria are potent antimicrobial peptides that inhibit pathogenic and food-spoilage bacteria. They are usually ineffective against gram-negative bacteria because they cannot penetrate the outer membrane (OM). Disruption of the OM of some gram-negative bacteria was reported to sensitize...

journal_title：FEMS microbiology letters

authors： Martin-Visscher LA,Yoganathan S,Sit CS,Lohans CT,Vederas JC

更新日期：2011-04-01 00:00:00

abstract：:This study describes how bkaR, a highly conserved mycobacterial TetR-like transcriptional repressor, regulates a number of nearby genes that have associations with branched-chain keto-acid metabolism. bkaR (MSMEG_4718) was deleted from the nonpathogenic species Mycobacterium smegmatis, and changes in global gene expre...

journal_title：FEMS microbiology letters

authors： Balhana RJ,Swanston SN,Coade S,Withers M,Sikder MH,Stoker NG,Kendall SL

更新日期：2013-08-01 00:00:00

abstract：:We report the draft genome sequence from Aeromonas salmonicida sp. strain CBA100, which was characterized as an antibiotic-resistant bacterium isolated from infected rainbow trout. The total size of the genome is 4,788,109 bp, with a G + C content of 60.55%. Comparison of its open reading frames shows that the closest...

journal_title：FEMS microbiology letters

authors： Valdes N,Espinoza C,Sanhueza L,Gonzalez A,Corsini G,Tello M

更新日期：2015-03-01 00:00:00

abstract：:Five independent collections, comprising a total of 34 clones encoding cellulases, hemicellulases and cell surface proteins of Clostridium thermocellum, were searched for overlapping or contiguous DNA fragments. The clones were hybridized to large genomic restriction fragments separated by pulse-field electrophoresis....

journal_title：FEMS microbiology letters

authors： Guglielmi G,Béguin P

更新日期：1998-04-01 00:00:00

abstract：:DNA from Aspergillus sp. has been reported not to contain 5-methylcytosine. However, it has been found that Aspergillus nidulans responds to 5-azacytidine, a drug that is a strong inhibitor of DNA methyltransferases. Therefore, we have re-examined the occurrence of 5-methylcytosine in DNA from Aspergillus flavus by us...

journal_title：FEMS microbiology letters

authors： Gowher H,Ehrlich KC,Jeltsch A

更新日期：2001-11-27 00:00:00

abstract：:Aspergillus parasiticus (255), a non-toxigenic isolate showed the presence of secondary metabolites-aflatoxins (B1, B2, G1, G2) when grown in yeast extract sucrose media but not in basal media, thus demonstrating its toxigenic potential. Native PAGE of the crude protein isolated at different growth periods of A. paras...

journal_title：FEMS microbiology letters

authors： Shashidhar J,Sashidhar RB,Deshpande V

更新日期：2005-10-01 00:00:00

abstract：:The polyhydroxyalkanoate (PHA) synthase (PhaC(Da)) from Delftia acidovorans DS-17 (formerly Comamonas acidovorans) has a unique large insertion consisting of 40 amino acid residues in the alpha/beta hydrolase fold region. In order to examine whether this insertion is necessary for enzyme function, we generated a mutan...

journal_title：FEMS microbiology letters

authors： Tsuge T,Imazu S,Takase K,Taguchi S,Doi Y

更新日期：2004-02-09 00:00:00

abstract：:The identification of virulence factors in Rhodococcus equi has been severely hampered by the lack of a method for in vivo random insertion mutagenesis. This study reports the use of transposomes to generate random insertions of a gene conferring kanamycin resistance into the genome of R. equi ATCC 33701. Southern hyb...

journal_title：FEMS microbiology letters

authors： Mangan MW,Meijer WG

更新日期：2001-12-18 00:00:00

abstract：:Production of the gene transfer agent of Rhodobacter capsulatus, RcGTA, is dependent upon several cellular regulatory systems, including a putative phosphorelay involving the CtrA and CckA proteins. These proteins are also involved in flagellar motility in R. capsulatus. The interactions of proteins in this system are...

journal_title：FEMS microbiology letters

authors： Mercer RG,Quinlan M,Rose AR,Noll S,Beatty JT,Lang AS

更新日期：2012-06-01 00:00:00

abstract：:Streptococcus pneumoniae strain JNR.7/87 is a highly virulent, type 4 encapsulated Gram-positive bacterium whose transformability has not been tested previously, and whose genome is currently being sequenced. The strain was transformed at very low efficiency by addition of exogenous competence-stimulating peptide: How...

journal_title：FEMS microbiology letters

authors： Bricker AL,Camilli A

更新日期：1999-03-15 00:00:00

abstract：:Acidithiobacillus ferrivorans is an acidophile implicated in low-temperature biomining for the recovery of metals from sulfide minerals. Acidithiobacillus ferrivorans obtains its energy from the oxidation of inorganic sulfur compounds, and genes encoding several alternative pathways have been identified. Next-generati...

journal_title：FEMS microbiology letters

authors： Christel S,Fridlund J,Buetti-Dinh A,Buck M,Watkin EL,Dopson M

更新日期：2016-04-01 00:00:00

abstract：:CheY, the response regulator of the chemotaxis system in Escherichia coli, can be regulated by two covalent modifications - phosphorylation and acetylation. Both covalent modifications are involved in chemotaxis, but the mechanism and role of the acetylation are still obscure. While acetylation was shown to repress th...

journal_title：FEMS microbiology letters

authors： Li R,Chen P,Gu J,Deng JY

更新日期：2013-10-01 00:00:00

abstract：:Atypical enteropathogenic Escherichia coli (EPEC) comprise an important group of paediatric pathogens. Atypical EPEC have reservoirs in farm and domestic animals where they can be either commensal or pathogenic; serogroup O26 is dominant in humans and animals. Central to intestinal colonization by EPEC is the transloc...

journal_title：FEMS microbiology letters

authors： Ooka T,Vieira MA,Ogura Y,Beutin L,La Ragione R,van Diemen PM,Stevens MP,Aktan I,Cawthraw S,Best A,Hernandes RT,Krause G,Gomes TA,Hayashi T,Frankel G

更新日期：2007-06-01 00:00:00

abstract：:The plant-growth promoting rhizobacterium Azospirillum lipoferum strain 4B generates in vitro a stable phase variant designated 4VI at frequencies of 10(-4) to 10(-3) per cell per generation. Variant 4VI displays pleitropic modifications, such as the loss of swimming motility and the inability to assimilate certain su...

journal_title：FEMS microbiology letters

authors： Vial L,Pothier JF,Normand P,Moënne-Loccoz Y,Bally R,Wisniewski-Dyé F

更新日期：2004-07-15 00:00:00

abstract：:A mixture of two lyso isomers of a galactolipid was obtained from Dictyonema glabratum. Aqueous hydrolysis gave rise to galactose and glycerol in a 3:1 molar ratio. ESI-MS spectroscopy gave, in the positive-ion mode, a pseudomolecular ion at m/z 839 and daughter ions with m/z 677, 600, 515 and 353, suggesting three ga...

journal_title：FEMS microbiology letters

authors： Sassaki GL,Gorin PA,Iacomini M

更新日期：2001-01-15 00:00:00

abstract：:A Deltalgt (Lgt, lipoprotein diacylglyceryl transferase) isogenic mutant was obtained which indicates that lgt is not essential for cell growth in vitro, like in the Gram-positive bacterium Bacillus subtilis, but unlike in the proteobacteria Escherichia coli and Salmonella typhimurium. The mutation was transduced to a...

journal_title：FEMS microbiology letters

authors： Petit CM,Brown JR,Ingraham K,Bryant AP,Holmes DJ

更新日期：2001-06-25 00:00:00

abstract：:Escherichia coli mutant MM52 (secA(ts)) was transformed with a cosmid library from Staphylococcus carnosus, and a recombinant cosmid (pBO23) allowing growth at the non-permissive temperature (42 degrees C) was isolated. pBO23 also restored the growth defects of E. coli mutants IQ85 (secY(ts)) and IT41 (lep(ts)). Nucle...

journal_title：FEMS microbiology letters

authors： Overhoff-Freundlieb B,Freudl R

更新日期：1991-11-15 00:00:00

abstract：:Antibacterial effects in terms of biofilm formation and swarming motility were studied using polyacrylate plates having protruding or recessed shark skin micropatterned surfaces with a shallow groove (2 μm pattern width and spacing, 0.4 μm pattern height). It was found that biofilm formation and swarming motility of P...

journal_title：FEMS microbiology letters

authors： Sakamoto A,Terui Y,Horie C,Fukui T,Masuzawa T,Sugawara S,Shigeta K,Shigeta T,Igarashi K,Kashiwagi K

更新日期：2014-12-01 00:00:00