Abstract:
:Carboxy (C)-terminal processing proteases (CTP) are a relatively new group of serine proteases. Found in a broad range of organisms - bacteria, archaea, algae, plants and animals - these proteases are involved in the C-terminal processing of proteins. In comparison with amino-terminal processing of bacterial proteins, less is known about C-terminal processing and its physiological function. Bacterial CTPs appear to influence different basal cellular processes. Although CTPs of Gram-negative bacteria are generally referred to as being localized in the periplasm, there is little experimental evidence for this. We show for the first time the subcellular localization of a CTP-3 family protein from Pseudomonas aeruginosa, named CtpA, in the periplasm by a carefully designed fractionation study. Our results provide experimental evidence for the generally accepted hypothesis that CTPs are located in the periplasmic space of Gram-negative bacteria.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Hoge R,Laschinski M,Jaeger KE,Wilhelm S,Rosenau Fdoi
10.1111/j.1574-6968.2010.02181.xsubject
Has Abstractpub_date
2011-03-01 00:00:00pages
23-30issue
1eissn
0378-1097issn
1574-6968journal_volume
316pub_type
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