The IS1 elements in Shigella boydii: horizontal transfer, vertical inactivation and target duplication.

abstract：:The emergence and dissemination of colistin resistance among Gram-negative bacteria is a global problem. We initiated a surveillance of colistin-resistant and -susceptible Escherichia coli in raw meats from chicken in Nepal. A total of 180 meat samples were collected; from these, 60 E. coli strains were isolated (33.3...

journal_title：FEMS microbiology letters

authors： Joshi PR,Thummeepak R,Leungtongkam U,Pooarlai R,Paudel S,Acharya M,Dhital S,Sitthisak S

更新日期：2019-10-01 00:00:00

abstract：:We report a rapid and reliable two-step multiplex polymerase chain reaction (PCR) assay to identify the 10 Bacteroides fragilis group species - Bacteroides caccae, B. distasonis, B. eggerthii, B. fragilis, B. merdae, B. ovatus, B. stercoris, B. thetaiotaomicron, B. uniformis and B. vulgatus. These 10 species were firs...

journal_title：FEMS microbiology letters

authors： Liu C,Song Y,McTeague M,Vu AW,Wexler H,Finegold SM

更新日期：2003-05-16 00:00:00

abstract：:The best yield of lipopolysaccharide (LPS) of Pseudomonas pseudomallei GIFU 12046 was obtained by extraction of defatted cells by phenol/chloroform/petroleum ether. The LPS showed a smooth character on SDS-polyacrylamide gel electrophoresis and contained D-glucose, L-glycero-D-manno-heptose, and D-glucosamine as the m...

journal_title：FEMS microbiology letters

authors： Kawahara K,Dejsirilert S,Danbara H,Ezaki T

更新日期：1992-09-15 00:00:00

abstract：:There are two major pathways for methionine biosynthesis: the enterobacterial type transsulfuration pathway and the sulfhydrylation pathway as previously identified in the spirochete Leptospira meyeri. Sequence analysis of the L. meyeri metYX locus allows the identification of a third gene, called metW, which encodes ...

journal_title：FEMS microbiology letters

authors： Picardeau M,Bauby H,Saint Girons I

更新日期：2003-08-29 00:00:00

abstract：:A physical and functional map of Aspergillus tubingensis mtDNA type 2b was constructed and compared to Aspergillus niger typeI a mtDNA. The gene content and order, as well as the patterns of restriction sites, were similar. Two unidentified ORFs and several repeat elements were found in the region between the ndhl and...

journal_title：FEMS microbiology letters

authors： Juhász A,Láday M,Gácser A,Kucsera J,Pfeiffer I,Kevei F,Hamari Z

更新日期：2004-12-01 00:00:00

abstract：:In Azotobacter chroococcum the hydrogenase structural genes (hupSL) cover about 2.8 kb of a 15-kb region associated with hydrogen-uptake (Hup) activity. Two other genes in this region, hupD and hupE, were located 8.9 kb downstream of hupL and were shown to be essential for hydrogenase activity by insertion mutagenesis...

journal_title：FEMS microbiology letters

authors： Du L,Stejskal F,Tibelius KH

更新日期：1992-09-01 00:00:00

abstract：:Ferric uptake regulator (Fur) proteins are widely recognized as repressors that in many prokaryotes regulate a large number of genes involved in iron homeostasis and oxidative stress response. In our study, we were able to identify the complete sequence of the fur gene from Microcystis aeruginosa using inverse-polymer...

journal_title：FEMS microbiology letters

authors： Martin-Luna B,Hernandez JA,Bes MT,Fillat MF,Peleato ML

更新日期：2006-01-01 00:00:00

abstract：:The streptokinase gene of Streptococcus equisimilis H46 was inactivated by plasmid insertion mutagenesis to study the relationship between elaboration of streptokinase and acquisition of cell-associated plasmin activity after incubation of wild-type and mutant cells in media containing plasminogen or plasmin. The resu...

journal_title：FEMS microbiology letters

authors： Malke H,Mechold U,Gase K,Gerlach D

更新日期：1994-02-01 00:00:00

abstract：:The effect of coumarin (1,2 benzopyrone) on glucose utilisation by the anaerobic rumen fungi Neocallimastix frontalis and N. patriciarum has been compared with the effect of p-coumaric acid. Both compounds largely inhibited glucose utilisation by N. patriciarum strain Cx when present in the medium at a concentration o...

journal_title：FEMS microbiology letters

authors： Cansunar E,Richardson AJ,Wallace G,Stewart CS

更新日期：1990-07-01 00:00:00

abstract：:Integrons are bacterial genetic elements capable of capturing and expressing potentially adaptive genetic material. Class 1 integrons constitute the most intensely studied group of these elements to date, mainly due to their well-established role in the acquisition and dissemination of antibiotic resistance genes in c...

journal_title：FEMS microbiology letters

authors： Hardwick SA,Stokes HW,Findlay S,Taylor M,Gillings MR

更新日期：2008-01-01 00:00:00

abstract：:Photorhabdus (Enterobacteriaceae) bacteria are pathogenic to insects and mutualistic with entomopathogenic Heterorhabditis nematodes. Photorhabdus luminescens subsp. akhurstii LN2, associated with Heterorhabditis indica LN2, shows nematicidal activity against H. bacteriophora H06 infective juveniles (IJs). In the pres...

journal_title：FEMS microbiology letters

authors： Qiu X,Wu C,Cao L,Ehlers RU,Han R

更新日期：2016-03-01 00:00:00

abstract：:Bacterial specific primers were used to amplify 23S rRNA genes from a representative strain from each of the 23 serogroups of the pathogenic Leptospira interrogans and 8 strains from 6 serogroups of the non-pathogenic Leptospira biflexa. Only regions of extreme variability, which had been identified on the basis of ho...

journal_title：FEMS microbiology letters

authors： Woo TH,Smythe LD,Symonds ML,Norris MA,Dohnt MF,Patel BK

更新日期：1997-05-01 00:00:00

abstract：:Three 16S rRNA-targeted oligonucleotide probes, namely, PSMg437 targeting several members of the genus Pseudomonas, Hlm474 targeting several members of the genus Halomonas, and Clw844 targeting several members of the genus Colwellia, were designed. The microbial community structure and nitrogen removal ability of nitr...

journal_title：FEMS microbiology letters

authors： Yoshie S,Noda N,Tsuneda S,Hirata A,Inamori Y

更新日期：2004-06-01 00:00:00

abstract：:To clarify the cause of the supersusceptibility of Branhamella catarrhalis to macrolide antibiotics, which are well-known to be inactive to most Gram-negative bacteria, we determined its cell surface hydrophobicity by the partition experiment between water and hydrocarbons. Its cell surface was found to be markedly mo...

journal_title：FEMS microbiology letters

authors： Gotoh N,Tanaka S,Nishino T

更新日期：1989-05-01 00:00:00

abstract：:The ADAMs are a family of integral membrane proteases involved in shedding and fusion events in animal tissues. Here, we report the identification of two ADAMs, ADM-A and ADM-B, in the pathogenic fungus Aspergillus fumigatus. The domain structure of metazoan ADAMs was seen in ADM-A and -B, although with some differenc...

journal_title：FEMS microbiology letters

authors： Lavens SE,Rovira-Graells N,Birch M,Tuckwell D

更新日期：2005-07-01 00:00:00

abstract：:An analysis of the 2 kb nucleotide sequence including the 5'-flanking region of a cell-adhesion protein-encoding gene (mfbA) isolated from the basidiomycete Lentinus edodes revealed that the promoter region contains a TATA box, a GC box, a CAAT box, a CT-rich sequence element, a TATA box, two CT-rich sequences, and a ...

journal_title：FEMS microbiology letters

authors： Kondoh O,Shishido K

更新日期：1995-08-01 00:00:00

abstract：:We have re-examined the stringent response of Streptococcus rattus and Streptococcus pyogenes, two organisms that had originally been reported not to accumulate ppGpp following amino acid deprivation. We conclude that ppGpp does accumulate when S. rattus and S. pyogenes are deprived of isoleucine by mupirocin addition...

journal_title：FEMS microbiology letters

authors： Whitehead KE,Webber GM,England RR

更新日期：1998-02-01 00:00:00

abstract：:In several bacterial species that show natural transformation, dprA has been described as a competence gene. The DprA protein has been suggested to be involved in the protection of incoming DNA. However, members of the dprA gene family (also called smf) can be detected in virtually all bacterial species, which suggest...

journal_title：FEMS microbiology letters

authors： Smeets LC,Becker SC,Barcak GJ,Vandenbroucke-Grauls CM,Bitter W,Goosen N

更新日期：2006-10-01 00:00:00

abstract：:The use of plasmids possessing a regulatable gene coding for a site-specific recombinase together with its recognition sequences significantly facilitates genome manipulations since it allows self-excision of the portion of the genetic construct integrated into the host genome. Stable maintenance of such plasmids in E...

journal_title：FEMS microbiology letters

authors： Agaphonov MO

更新日期：2017-12-01 00:00:00

abstract：:Piscirickettsia salmonis is a novel, aggressive, facultative Gram-negative bacterium that drastically affects salmon production at different latitudes, with particular impact in southern Chile. Initially, P. salmonis was described as a Rickettsia-like, obligate, intracellular Alphaproteobacteria, but it was reclassifi...

journal_title：FEMS microbiology letters

authors： Marshall SH,Henríquez V,Gómez FA,Cárdenas C

更新日期：2011-01-01 00:00:00

abstract：:We determined the complete nucleotide sequence of the 16341 bp plasmid pHH205 of the extremely halophilic archaeon Halobacterium salinarum J7. The plasmid has a G+C content of 61.1%. A number of direct and inverted repeat sequences were found in pHH205, while no insertion sequences were found. Thirty-eight large open ...

journal_title：FEMS microbiology letters

authors： Ye X,Ou J,Ni L,Shi W,Shen P

更新日期：2003-04-11 00:00:00

abstract：:In the Pseudomonas fluorescens strain ST the ability to utilize styrene as the sole carbon source is due to the presence of a plasmid, pEG. In the present report we show that pEG contains two inverted repeat sequences and we present evidence indicating that the catabolic genes are localized in these repeats. The regio...

journal_title：FEMS microbiology letters

authors： Ruzzi M,Zennaro E

更新日期：1989-06-01 00:00:00

abstract：:The filamentous fungus Cunninghamella elegans has the ability to metabolize xenobiotics, including polycyclic aromatic hydrocarbons and pharmaceutical drugs, by both phase I and II biotransformations. Cytosolic and microsomal fractions were assayed for activities of cytochrome P450 monooxygenase, aryl sulfotransferase...

journal_title：FEMS microbiology letters

authors： Zhang D,Yang Y,Leakey JE,Cerniglia CE

更新日期：1996-05-01 00:00:00

abstract：:Type II restriction endonucleases were purified by heparin-sepharose followed by ion chromatography from Treponema strains. The results indicate that in addition to frequently cutting GATC-specific restriction enzymes, the tested strains also possess rarely cutting endonucleases. The purified restriction endonucleases...

journal_title：FEMS microbiology letters

authors： Piknova M,Javorsky P,Pristas P

更新日期：2005-10-01 00:00:00

abstract：:The beta-toxin gene from Clostridium perfringens type C was cloned and expressed as a glutathione S-transferase fusion protein in Escherichia coli. The DNA sequence was determined and compared to the type B sequence. Two nucleotide differences were found in the protein coding sequence, resulting in one amino acid diff...

journal_title：FEMS microbiology letters

authors： Steinthorsdottir V,Fridriksdottir V,Gunnarsson E,Andrésson OS

更新日期：1995-08-01 00:00:00

abstract：:The malate dehydrogenase (MDH; EC 1.1.1.37; L-malate-NAD(+)-oxidoreductase) activities of truffles of the genus Tuber (Tuber melanosporum Vittad., Tuber brumale Vittad., Tuber aestivum Vittad., Tuber magnatum Pico, Tuber rufum Pico) have been characterized with regard to the K(m) and V(max) values in the direct and re...

journal_title：FEMS microbiology letters

authors： Zarivi O,Cesare P,Aimola P,Ragnelli AM,Scirri C,Cimini A,Bonfigli A,Pacioni G,Miranda M

更新日期：2000-04-15 00:00:00

abstract：:Characterization of genomic variation among different microbial species, or different strains of the same species, is a field of significant interest with a wide range of potential applications. We have investigated the genomic variation in mycorrhizal fungal genomes through genomic suppressive subtractive hybridizati...

journal_title：FEMS microbiology letters

authors： Murat C,Zampieri E,Vallino M,Daghino S,Perotto S,Bonfante P

更新日期：2011-05-01 00:00:00

abstract：:Type II toxin-antitoxin systems (TAs) are small autoregulated bicistronic operons that encode a toxin protein with the potential to inhibit metabolic processes and an antitoxin protein to neutralize the toxin. Most of the bacterial genomes encode multiple TAs. However, the diversity and accumulation of TAs on bacteria...

journal_title：FEMS microbiology letters

authors： Ramisetty BC,Santhosh RS

更新日期：2016-02-01 00:00:00

abstract：:The DnaK chaperone system is involved in various cellular processes such as the control of the folded and oligomeric state of proteins under stress and non-stress conditions. In this study we functionally characterised the homologues of the DnaK system from Clostridium acetobutylicum DnaK, DnaJ, GrpE and OrfA were het...

journal_title：FEMS microbiology letters

authors： Rüngeling E,Laufen T,Bahl H

更新日期：1999-01-01 00:00:00

abstract：:The detection of Trypanosoma cruzi kinetoplast DNA by polymerase chain reaction (PCR) amplification is a potentially powerful tool for the parasitological diagnosis of Chagas' disease. We have applied this technique in a field situation in Bolivia, where 45 children from a primary school were subjected to serological ...

journal_title：FEMS microbiology letters

authors： Wincker P,Bosseno MF,Britto C,Yaksic N,Cardoso MA,Morel CM,Brenière SF

更新日期：1994-12-15 00:00:00