Functional characterisation of the chaperones DnaK, DnaJ, and GrpE from Clostridium acetobutylicum.

abstract：:The chimeric plasmid pMY10 containing the origin of replication of pAL5000, the origin of replication of pBR322, the origin of transfer of pRK2 and a kanamycin resistance gene was constructed and successfully transferred by conjugation from Escherichia coli harbouring the helper plasmid pRK4.24 into Mycobacterium smeg...

journal_title：FEMS microbiology letters

authors： Lazraq R,Clavel-Sérès S,David HL,Roulland-Dussoix D

更新日期：1990-05-01 00:00:00

abstract：:The biosynthesis of glyantrypine from radiolabelled amino acid precursors has been shown experimentally to involve anthranilic acid, tryptophan and glycine. Low values for percentage incorporation of radiolabel into glyantrypine were partly influenced by a complex array of other novel alkaloids shown by the radiolabel...

journal_title：FEMS microbiology letters

authors： Penn J,Purcell M,Mantle PG

更新日期：1992-05-01 00:00:00

abstract：:Acinetobacter sp. strain 20B was isolated based on the ability to utilize dimethyl sulfide as the sole sulfur source. Since strain 20B oxidized indole as well as dimethyl sulfide, indigo production by recombinant Escherichia coli clones carrying Acinetobacter DNA was used as a selection for cloning genes encoding dime...

journal_title：FEMS microbiology letters

authors： Horinouchi M,Kasuga K,Nojiri H,Yamane H,Omori T

更新日期：1997-10-01 00:00:00

abstract：:Pseudomonas aeruginosa PA96 is a clinical isolate from Guangzhou, China, that is multiresistant to antibiotics. We previously described the 500-kb IncP-2 plasmid, pOZ176 that encodes many resistance genes including the IMP-9 carbapenemase. Whole-genome sequencing of PA96 enabled characterization of its genomic islands...

journal_title：FEMS microbiology letters

authors： Déraspe M,Alexander DC,Xiong J,Ma JH,Low DE,Jamieson FB,Roy PH

更新日期：2014-07-01 00:00:00

abstract：:Listeria monocytogenes is a Gram positive pathogen that is ubiquitous in the environment. It is a facultative anaerobic rod that causes listeriosis, a disease with potentially lethal consequences for susceptible individuals. During infection, the pathogen is capable of sequestering metal ions to act as vital biocataly...

journal_title：FEMS microbiology letters

authors： Dowd GC,Casey PG,Begley M,Hill C,Gahan CG

更新日期：2012-02-01 00:00:00

abstract：:Three heterocytous cyanobacterial strains were isolated from different habitats of Central India, and initial morphological studies indicated them to be members of the genus Nostoc or other closely related genera. Subsequent studies using morphological, ecological, molecular and phylogenetic methods indicated the thre...

journal_title：FEMS microbiology letters

authors： Saraf A,Dawda HG,Suradkar A,Behere I,Kotulkar M,Shaikh ZM,Kumat A,Batule P,Mishra D,Singh P

更新日期：2018-12-01 00:00:00

abstract：:Carbohydrate-binding components were shown to be present at the surface of Listeria monocytogenes by means of a panel of neoglycoproteins using direct agglutination. These lectin-like components bind on neoglycoproteins bearing D-glucosamine, L-fucosylamine, or para-amino-phenyl-alpha-D-mannopyrannoside residues. The ...

journal_title：FEMS microbiology letters

authors： Cottin J,Loiseau O,Robert R,Mahaza C,Carbonnelle B,Senet JM

更新日期：1990-03-15 00:00:00

abstract：:The transcriptional repressor CcpN from Bacillus subtilis mediates the CcpA-independent catabolite repression of three genes, sr1, encoding a small regulatory RNA, and two gluconeogenesis genes, gapB and pckA. The intracellular concentration of CcpN was determined to be around 4000 molecules per cell. The B. subtilis ...

journal_title：FEMS microbiology letters

authors： Eckart RA,Brantl S,Licht A

更新日期：2009-10-01 00:00:00

abstract：:Pseudomonas syringae pv. tomato DC3000, a plant pathogenic gram-negative bacterium, employs the type III secretion system (T3SS) to cause disease in tomato and Arabidopsis and to induce the hypersensitive response in nonhost plants. The expression of T3SS is regulated by the HrpL extracytoplasmic sigma factor. Express...

journal_title：FEMS microbiology letters

authors： Jovanovic M,Lawton E,Schumacher J,Buck M

更新日期：2014-07-01 00:00:00

abstract：:The Saccharomyces sensu stricto genus contains many species that are industrially important for fermentation of wines, beers and ales. The molecular characterization of the genomes of yeasts involved in these processes reveals that the majority arose from interspecific hybridization between two and sometimes three yea...

journal_title：FEMS microbiology letters

authors： Querol A,Bond U

更新日期：2009-04-01 00:00:00

abstract：:Bordetella pertussis is the causative agent of whooping cough. Traditional vaccines against this disease are inherently reactogenic, thus research is currently focussed on the production of less reactive, acellular vaccines. Expression of candidate antigens for these vaccines in Escherichia coli would be preferable, h...

journal_title：FEMS microbiology letters

authors： Smith AM,Yan H,Groves N,Dalla Pozza T,Walker MJ

更新日期：2000-10-15 00:00:00

abstract：:In the purple sulphur bacterium Allochromatium vinosum, the prosthetic group of dissimilatory sulphite reductase (DsrAB) was identified as siroamide, an amidated form of the classical sirohaem. The genes dsrAB are the first two of a large cluster of genes necessary for the oxidation of sulphur globules stored intracel...

journal_title：FEMS microbiology letters

authors： Lübbe YJ,Youn HS,Timkovich R,Dahl C

更新日期：2006-08-01 00:00:00

abstract：:Two interlinked quorum sensing circuits, las and rhl, which control pathogenesis of Pseudomonas aeruginosa are further modulated by numerous regulators, including VqsR (virulence and quorum sensing regulator). High-density oligonucleotide microarrays were used to compare the global expression profile of a wild-type an...

journal_title：FEMS microbiology letters

authors： Juhas M,Wiehlmann L,Salunkhe P,Lauber J,Buer J,Tümmler B

更新日期：2005-01-15 00:00:00

abstract：:Polyclonal antibodies were prepared against the major antenna chlorophyll (Chl) a/b-binding protein from the prokaryote Prochlorothrix hollandica (Burger-Wiersma et al. (1986) Nature (Lond.) 320, 262-264). Immunoblotting experiments on Triton X-114 phase-partitioned P. hollandica thylakoids revealed that the antibody ...

journal_title：FEMS microbiology letters

authors： Bullerjahn GS,Jensen TC,Sherman DM,Sherman LA

更新日期：1990-01-15 00:00:00

abstract：:During protein synthesis, elongation factor G (EFG) participates at the steps of translocation and ribosome recycling. Fusidic acid (FA) is a bacteriostatic antibiotic, which traps EFG on ribosomes, stalling them on mRNAs. How the bacterial susceptibility to FA is determined, and which of the two functions of EFG (tra...

journal_title：FEMS microbiology letters

authors： Ayyub SA,Lahry K,Dobriyal D,Mondal S,Varshney U

更新日期：2018-07-01 00:00:00

abstract：:Expression of the dimethylsulfoxide respiratory (dor) operon of Rhodobacter is regulated by oxygen, light intensity and availability of substrate. Since dimethylsulfoxide reductase contains a pterin molybdenum cofactor, the role of molybdate in the regulation of dor operon expression was investigated. In this report w...

journal_title：FEMS microbiology letters

authors： Solomon PS,Shaw AL,Young MD,Leimkuhler S,Hanson GR,Klipp W,McEwan AG

更新日期：2000-09-15 00:00:00

abstract：:Giardia lamblia is a binucleated, flagellated protozoan parasite that inhabits the upper small intestine of its vertebrate hosts. The entire life cycle, which can be completed in vitro, is simple with cycling between a vegetative trophozoite and a highly resistant cystic form. The parasite is one of the earliest diver...

journal_title：FEMS microbiology letters

authors： Svärd SG,Hagblom P,Palm JE

更新日期：2003-01-21 00:00:00

abstract：:The first method for enucleation of yeast Saccharomyces cerevisiae is reported. Various strains, including some killer strain and respiratory-deficient mutants of Saccharomyces cerevisiae were enucleated after treatment with cytochalasin B. Removal of nuclei from protruding sphaeroplasts was induced by centrifugation ...

journal_title：FEMS microbiology letters

authors： Salek AT

更新日期：1993-10-01 00:00:00

abstract：:Members of the Streptococcus bovis group are frequent colonizers of the intestinal tract, which can also cause endocarditis. However, their ability to adhere to and colonize host tissues and the factors associated with pathogenicity are largely unknown. Here, we assessed 17 endocarditis-derived human isolates [identif...

journal_title：FEMS microbiology letters

authors： Sillanpää J,Nallapareddy SR,Singh KV,Ferraro MJ,Murray BE

更新日期：2008-12-01 00:00:00

abstract：:In artificial engineered systems, nitrification is a key reaction that accounts for the removal of biological nitrogen. Recently, a single microbe capable of oxidizing ammonia to nitrate, known as a complete ammonia oxidizer (comammox), has been discovered. Although the abundance and diversity of comammox Nitrospira i...

journal_title：FEMS microbiology letters

authors： Fujitani H,Nomachi M,Takahashi Y,Hasebe Y,Eguchi M,Tsuneda S

更新日期：2020-01-01 00:00:00

abstract：:Two-component systems are widespread in nature and constitute the most common mechanism of transmembrane signal transduction in bacteria. Recently, a functionally active two-component system consisting of malS and malR genes possibly involved in the expression of a C4-dicarboxylate transporter system (dctAB operon) wa...

journal_title：FEMS microbiology letters

authors： Papa R,Rippa V,Duilio A

更新日期：2009-06-01 00:00:00

abstract：:In previous work, only one culture (strain TA12) from a pristine site was reported to utilize the xenobiotic compound p-toluenesulfonate (TSA) as a sole source of carbon and energy for aerobic growth. 'Strain TA12' has now been recognized as a community of three bacteria: Achromobacter xylosoxidans TA12-A, Ensifer adh...

journal_title：FEMS microbiology letters

authors： Tralau T,Yang EC,Tralau C,Cook AM,Küpper FC

更新日期：2011-03-01 00:00:00

abstract：:To explore the discriminatory power of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for detecting subtle differences in isogenic isolates, we tested isogenic strains of Staphylococcus aureus differing in their expression of resistance to methicillin or teicoplanin. More i...

journal_title：FEMS microbiology letters

authors： Majcherczyk PA,McKenna T,Moreillon P,Vaudaux P

更新日期：2006-02-01 00:00:00

abstract：:The sacB gene of Bacillus subtilis was successfully applied in various Arthrobacter, Brevibacterium, Corynebacterium and Rhodococcus strains for the isolation of transposable elements. Three different insertion sequence (IS) elements entrapped in sacB were isolated. The IS elements IS-Bl and IS-Cg isolated from Brevib...

journal_title：FEMS microbiology letters

authors： Jäger W,Schäfer A,Kalinowski J,Pühler A

更新日期：1995-02-01 00:00:00

abstract：:Endoglucanase of Aspergillus niger AS-101 was partially purified by ammonium sulphate fractionation and molecular sieving on Sephadex G-200. The enzyme was found to be unstable on storage, however, it received some protection on the addition of BSA, glycerol or sodium azide. Glycerol also protected the enzyme from ina...

journal_title：FEMS microbiology letters

authors： Singh A,Agrawal AK,Abidi AB,Darmwal NS

更新日期：1990-09-01 00:00:00

abstract：:A synthetic version of the ligA gene encoding the NAD-dependent DNA ligase from the Arctic bacterium Pseudoalteromonas haloplanktis was substituted for its homolog in the chromosome of Mycobacterium smegmatis. The resulting recombinant strain grew identically to the parent strain at permissive temperatures but failed ...

journal_title：FEMS microbiology letters

authors： Pinto CT,Nano FE

更新日期：2015-09-01 00:00:00

abstract：:The aim of this study was to illustrate the relative pervasiveness of Borderline Oxacillin Resistant Staphylococcus aureus (BORSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) in community and food of animal origin and their relationship with other genetic determinants. Staphylococcus aureus isolates were su...

journal_title：FEMS microbiology letters

authors： Zehra A,Gulzar M,Singh R,Kaur S,Gill JPS

更新日期：2020-12-22 00:00:00

abstract：:The phosphatidylcholine (PC) content of Aspergillus nidulans choC was varied by growing the auxotroph in medium containing various concentrations of choline chloride. Direct linear correlations were observed between PC content and in vivo chitin synthase activity, between in vivo chitin synthase activity and mean hyph...

journal_title：FEMS microbiology letters

authors： Binks PR,Robson GD,Goosey MW,Trinci AP

更新日期：1991-10-01 00:00:00

abstract：:Microbial competition for carbon sources is a primary determinant of rhizosphere ecology. We employed the PCR to examine the population fluctuations of a symbiotic nitrogen-fixing bacterium (Rhizobium etli) during the first 11 days following inoculation of Phaseolus vulgaris seedlings grown in the presence or absence ...

journal_title：FEMS microbiology letters

authors： Petersen DJ,Srinivasan M,Chanway CP

更新日期：1996-09-01 00:00:00

abstract：:The effect of low concentrations of cyanide on dissimilatory perchlorate and chlorate reduction and aerobic respiration was examined using pure cultures of Azospira sp. KJ. Cyanide at a concentration of 38 microM inhibited cell growth on perchlorate, chlorate and molecular oxygen, but it did not inhibit the activity o...

journal_title：FEMS microbiology letters

authors： Song Y,Logan BE

更新日期：2004-10-15 00:00:00