Design of 16S rRNA-targeted oligonucleotide probes and microbial community analysis in the denitrification process of a saline industrial wastewater treatment system.

abstract：:Primers designed to conserved regions of botulinum and tetanus clostridial toxins were used to amplify DNA fragments from non-proteolytic Clostridium botulinum type F (202F) DNA using polymerase chain reaction technology. The fragments were cloned and the complete nucleotide sequence of the gene encoding type F toxin ...

journal_title：FEMS microbiology letters

authors： East AK,Richardson PT,Allaway D,Collins MD,Roberts TA,Thompson DE

更新日期：1992-09-15 00:00:00

abstract：:The nucleotide sequence of a 714-bp DNA fragment containing the enterotoxic Escherichia coli (ETEC) adhesive factor 8786 structural gene, designated nfaA, revealed an open reading frame of 498 bp encoding a polypeptide of 166 amino acids. Primer-extension experiments showed that the nfaA gene is within a single transc...

journal_title：FEMS microbiology letters

authors： Aubel D,Darfeuille-Michaud A,Martin C,Joly B

更新日期：1992-11-01 00:00:00

abstract：:We have constructed a fungal subtractive suppressive library enriched in genes expressed during appressorium maturation in Magnaporthe grisea. Sequencing of 250 clones from the subtracted appressorium cDNA library revealed 142 unique genes, represented by 155 non-redundant ESTs (expressed sequence tags). Of these ESTs...

journal_title：FEMS microbiology letters

authors： Lu JP,Liu TB,Lin FC

更新日期：2005-04-01 00:00:00

abstract：:Plant protection via disease-suppressive bacteria in desert farming requires specific biological control agents (BCAs) adapted to the unique arid conditions. We performed an ecological study of below-ground communities in desert farm soil and untreated desert soil, and based on these findings, selected antagonists wer...

journal_title：FEMS microbiology letters

authors： Köberl M,Ramadan EM,Adam M,Cardinale M,Hallmann J,Heuer H,Smalla K,Berg G

更新日期：2013-05-01 00:00:00

abstract：:We transformed a clinical Staphylococcus epidermidis isolate with the Enterococcus faecalis transposon Tn917-carrying plasmid pTV1. Loss of plasmid replication was observed at 47 degrees C. Tn917 transposes efficiently and apparently randomly. The transposition frequency could be stimulated by erythromycin. Transposon...

journal_title：FEMS microbiology letters

authors： Grueter L,Koenig O,Laufs R

更新日期：1991-08-01 00:00:00

abstract：:Bacillus cereus strains involved in food poisoning cases of the diarrheal type may produce two different enterotoxin complexes. To facilitate the identification of hemolysin BL-enterotoxin complex (HBL) and/or the nonhemolytic enterotoxin (NHE) producing colonies a colony immunoblot procedure was developed, which allo...

journal_title：FEMS microbiology letters

authors： Moravek M,Wegscheider M,Schulz A,Dietrich R,Bürk C,Märtlbauer E

更新日期：2004-09-01 00:00:00

abstract：:Members of the Streptococcus bovis group are frequent colonizers of the intestinal tract, which can also cause endocarditis. However, their ability to adhere to and colonize host tissues and the factors associated with pathogenicity are largely unknown. Here, we assessed 17 endocarditis-derived human isolates [identif...

journal_title：FEMS microbiology letters

authors： Sillanpää J,Nallapareddy SR,Singh KV,Ferraro MJ,Murray BE

更新日期：2008-12-01 00:00:00

abstract：:A DNA segment located immediately upstream of the eaeA gene of enterohemorrhagic Escherichia coli O157:H7 strain HA1 was cloned and sequenced. This segment contained an open reading frame encoding a predicted protein of 156 amino acids. A database search identified similar open reading frames upstream of the eaeA gene...

journal_title：FEMS microbiology letters

authors： Zhao S,Mitchell SE,Meng J,Doyle MP,Kresovich S

更新日期：1995-11-01 00:00:00

abstract：:We present the results of a phylogenetic study, based on amplified ribosomal DNA restriction analysis of the rDNA operon, of 37 Arthrospira ('Spirulina') cultivated clonal strains from four continents. In addition, duplicates from different culture collections or markedly different morphotypes of particular strains es...

journal_title：FEMS microbiology letters

authors： Scheldeman P,Baurain D,Bouhy R,Scott M,Mühling M,Whitton BA,Belay A,Wilmotte A

更新日期：1999-03-15 00:00:00

abstract：:The filamentous fungus Fusarium lateritium is cyanide tolerant, due partly to the induction of the enzyme cyanide hydratase in the presence of cyanide. This enzyme catalyses the hydration of cyanide to formamide. The expression in Escherichia coli of a cDNA clone encoding cyanide hydratase is described. The cDNA clone...

journal_title：FEMS microbiology letters

authors： Brown DT,Turner PD,O'Reilly C

更新日期：1995-12-15 00:00:00

abstract：:Multidrug resistance plasmids carrying the bla(CMY-2) gene have been identified in Salmonella enterica serovars Typhimurium and Newport from the United States. This gene confers decreased susceptibility to ceftriaxone, and is most often found in strains with concomitant resistance to ampicillin, chloramphenicol, strep...

journal_title：FEMS microbiology letters

authors： Doublet B,Carattoli A,Whichard JM,White DG,Baucheron S,Chaslus-Dancla E,Cloeckaert A

更新日期：2004-04-15 00:00:00

abstract：:Two clones, obtained by polymerase chain reaction from macronuclear DNA of the unicellular ciliated protist Blepharisma japonicum, were isolated and sequenced. They correspond to fragments of two different putative H4 histone genes. The existence of multiple H4 histone genes was also suggested by Southern blot hybridi...

journal_title：FEMS microbiology letters

authors： Salvini M,Bini E,Santucci A,Batistoni R

更新日期：1997-04-01 00:00:00

abstract：:The sacB gene of Bacillus subtilis was successfully applied in various Arthrobacter, Brevibacterium, Corynebacterium and Rhodococcus strains for the isolation of transposable elements. Three different insertion sequence (IS) elements entrapped in sacB were isolated. The IS elements IS-Bl and IS-Cg isolated from Brevib...

journal_title：FEMS microbiology letters

authors： Jäger W,Schäfer A,Kalinowski J,Pühler A

更新日期：1995-02-01 00:00:00

abstract：:A lipopolysaccharide mutant of Leptospira interrogans (LaiMut) was obtained by growth in the presence of an agglutinating monoclonal antibody (mAb) against lipopolysaccharide. Agglutination reactions with anti-lipopolysaccharide mAbs and polyclonal antibodies showed that LaiMut had lost some serogroup Icterohaemorrhag...

journal_title：FEMS microbiology letters

authors： Zapata S,Trueba G,Bulach DM,Boucher D,Adler B,Hartskeerl R

更新日期：2010-08-01 00:00:00

abstract：:Poultry is one of the most consumed meats worldwide and there are many different types of poultry products available on the market for consumers. Many poultry producers provide a variety of labeled meats based on rearing system such as organic, free-range and antibiotic-free. However, these labels often can be mislead...

journal_title：FEMS microbiology letters

authors： Lee SI,Choi J,Daeschel DZ,Park SH

更新日期：2019-05-01 00:00:00

abstract：:Cholera epidemics caused by Vibrio cholerae 01 continue to represent a major public health concern in many developing countries. A rapid and simple test kit for the detection of V. cholerae 01 has been developed. The kit, CholeraScreen is a monoclonal antibody-based, co-agglutination test and is used directly with sto...

journal_title：FEMS microbiology letters

authors： Colwell RR,Hasan JA,Huq A,Loomis L,Siebeling RJ,Torres M,Galvez S,Islam S,Tamplin MT,Bernstein D

更新日期：1992-10-15 00:00:00

abstract：:The study compared images of mature Streptococcus mutans biofilms captured at increasing magnification to determine which microscopy method is most acceptable for imaging the biofilm topography and the extracellular polymeric substance (EPS). In vitro S. mutans biofilms were imaged using (1) scanning electron microsco...

journal_title：FEMS microbiology letters

authors： Weber K,Delben J,Bromage TG,Duarte S

更新日期：2014-01-01 00:00:00

abstract：:This paper describes the validation and application of a simple flask-based (14)C-respirometer system designed to assess mineralisation of (14)C-labelled substrates under defined conditions. Validation of this respirometer system indicated stoichiometric CO(2) trapping up to a maximum of 400 micromol of CO(2) (in a si...

journal_title：FEMS microbiology letters

authors： Reid BJ,MacLeod CJ,Lee PH,Morriss AW,Stokes JD,Semple KT

更新日期：2001-03-15 00:00:00

abstract：:The Fe(III) reductase activity was studied in the South African Fe(III)-reducing bacterium, Thermus scotoductus (SA-01). Fractionation studies revealed that the membrane as well as the soluble fraction contained NAD(P)H-dependent Fe(III) reductase activity. The membrane-associated enzyme was solubilized by KCl treatme...

journal_title：FEMS microbiology letters

authors： Möller C,van Heerden E

更新日期：2006-12-01 00:00:00

abstract：:Streptococcus mutans, the etiologic agent of dental caries in humans, is considered a dominating force in the oral microbiome due to its highly-evolved propensity for survival. The oral pathogen encodes an elaborate array of regulatory elements, including the carbon catabolite-responsive regulator, CcpA, a global regu...

journal_title：FEMS microbiology letters

authors： Lindsay EL,Faustoferri RC,Quivey RG

更新日期：2021-01-16 00:00:00

abstract：:Enteroaggregative Escherichia coli (EAEC) is an important diarrhoeal pathogen causing diseases in multiple epidemiological and clinical settings. In developing countries like India, diarrhoeal diseases are one of the major killers among paediatric population and oddly, few studies are available from Indian paediatric ...

journal_title：FEMS microbiology letters

authors： Gupta D,Sharma M,Sarkar S,Thapa BR,Chakraborti A

更新日期：2016-09-01 00:00:00

abstract：:Streptococcus mutans and certain other oral lactic-acid bacteria were found to have the ability to carry out malolactic fermentation involving decarboxylation of L-malate to yield L-lactic acid and concomitant reduction in acidity. The activity was inducible by L-malate in S. mutans UA159 growing in suspensions or bio...

journal_title：FEMS microbiology letters

authors： Sheng J,Marquis RE

更新日期：2007-07-01 00:00:00

abstract：:Lactobacilli occupy specific ecological niches, where they represent a major component of foods, human and animal microbial communities. Employing these bacteria in industrial fermentations or for human health benefits exposes them to certain life-threatening conditions where their ability to adapt plays a key role in...

journal_title：FEMS microbiology letters

authors： Hussain MA,Hosseini Nezhad M,Sheng Y,Amoafo O

更新日期：2013-12-01 00:00:00

abstract：:Two plasmids, pEM4 (IncFI) determining virulence and pEM6 (IncI) determining colicine I production and resistance to tetracycline, have been found in a pathogenic strain of Citrobacter freundii. Even though pEM4 and pEM6 plasmids are conjugative and transfer themselves very efficiently during conjugation, a high cotra...

journal_title：FEMS microbiology letters

authors： Morzejko E,Panek E,Cebrat S

更新日期：1990-03-01 00:00:00

abstract：:The detection of ancient microbial DNA offers a new approach for the study of infectious diseases, their occurrence, frequency and host-pathogen interaction in historic times and populations. Moreover, data obtained from skeletal and mummified tissue may represent an important completion of contemporary phylogenetic a...

journal_title：FEMS microbiology letters

authors： Zink AR,Reischl U,Wolf H,Nerlich AG

更新日期：2002-08-06 00:00:00

abstract：:The molecular genetics of indole-3-acetic (IAA) synthesis and regulation in Azospirillum brasilense was investigated in this study. Tn5 mutagenesis was performed and five mutants with decreased IAA production were isolated. Five Tn5-inserted genes from these mutants were cloned and sequenced. Four genes were reported ...

journal_title：FEMS microbiology letters

authors： Xie B,Xu K,Zhao HX,Chen SF

更新日期：2005-07-01 00:00:00

abstract：:Strains of the filamentous gliding bacterium Vitreoscilla, LB13 and C1, are shown to be highly sensitive to UV-A (320-400 nm), with an LD50 of less than 20 kJ m(-2). Vitreoscilla LB13 can be protected from UV-A by including superoxide dismutase and catalase, separately or in combination, during the exposure, indicatin...

journal_title：FEMS microbiology letters

authors： Biggs LF,Moody AJ

更新日期：2003-07-15 00:00:00

abstract：:A newly isolated strain of Variovorax paradoxus could grow on homovanillate and several monohydroxylated phenylacetic acids. During growth on homovanillate, the organism formed separate NAD(P)H-dependent hydroxylases with activity towards 4-hydroxyphenylacetic acid and homovanillate. Homovanillate hydroxylase catalyse...

journal_title：FEMS microbiology letters

authors： Allison N,Turner JE,Wait R

更新日期：1995-12-15 00:00:00

abstract：:Species of strictly and facultatively anaerobic cellulolytic bacteria from soils and sediments were examined for the ability to degrade chitin. Of 22 species studied, 16 degraded insoluble chitin. Cellulomonas uda, which was selected for a comparative study of its cellulase and chitinase enzyme systems, produced diffe...

journal_title：FEMS microbiology letters

authors： Reguera G,Leschine SB

更新日期：2001-11-13 00:00:00

abstract：:To test the hypothesis that actA allelic variation contributes to virulence differences among Listeria monocytogenes strains, cell-to-cell spread and intracellular ActA phosphorylation patterns were characterized for 14 wild-type isolates and selected isogenic mutants. Our data show that (i) while actA allelic variati...

journal_title：FEMS microbiology letters

authors： Roberts AJ,Wiedmann M

更新日期：2006-01-01 00:00:00