Extraction and characterization of lipopolysaccharide from Pseudomonas pseudomallei.

Abstract:

:The best yield of lipopolysaccharide (LPS) of Pseudomonas pseudomallei GIFU 12046 was obtained by extraction of defatted cells by phenol/chloroform/petroleum ether. The LPS showed a smooth character on SDS-polyacrylamide gel electrophoresis and contained D-glucose, L-glycero-D-manno-heptose, and D-glucosamine as the main sugar components, and 3-hydroxypalmitic acid as an amide-linked fatty acid. The growth conditions did not affect the electrophoresis profile and chemical composition of LPS. 2-Keto-3-deoxyoctonic acid was not detectable, and mild acid hydrolysis could not liberate free lipid A, suggesting that the linkage between inner core and lipid A was stable against acid hydrolysis, and the structure of this region is similar to that of P. cepacia, which has close taxonomic relationship with P. pseudomallei.

journal_name

FEMS Microbiol Lett

authors

Kawahara K,Dejsirilert S,Danbara H,Ezaki T

doi

10.1016/0378-1097(92)90392-2

keywords:

subject

Has Abstract

pub_date

1992-09-15 00:00:00

pages

129-33

issue

2-3

eissn

0378-1097

issn

1574-6968

journal_volume

75

pub_type

杂志文章
  • Stereoselective biosynthesis of chloroarylpropane diols by the basidiomycete Bjerkandera adusta: exploring the roles of amino acids, pyruvate, glycerol and phenyl acetyl carbinol.

    abstract::Bjerkandera adusta produces many chlorometabolites including chlorinated anisyl metabolites (CAMs) and 1-arylpropane-1,2-diols (1, 2, 3, 4) as idiophasic metabolic products of L-phenylalanine. These diols are stereoselectively biosynthesized from a C7-unit (benzylic, from L-phenylalanine) and a C2-unit, of unknown ori...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00725-0

    authors: Silk PJ,Macaulay JB

    更新日期:2003-11-07 00:00:00

  • Rapid accumulation of intracellular 2-keto-3-deoxy-6-phosphogluconate in an Entner-Doudoroff aldolase mutant results in bacteriostasis.

    abstract::The accumulation of 2-keto-3-deoxy-6-phosphogluconate, the key intermediate of the Entner-Doudoroff pathway, has long been thought to inhibit growth of bacteria, but careful measurements of 2-keto-3-deoxy-6-phosphogluconate accumulation by growing cells and the correlation of intracellular 2-keto-3-deoxy-6-phosphogluc...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb12870.x

    authors: Fuhrman LK,Wanken A,Nickerson KW,Conway T

    更新日期:1998-02-15 00:00:00

  • Ca2+ stabilization of respiratory complex I from Escherichia coli.

    abstract::Stability of the membrane-bound and purified H+-translocating NADH:ubiquinone oxidoreductase, Complex I, was studied. The loss of the enzyme activity is strongly increased by alkaline pH and dilution of the sample. Complex I inactivation is prevented specifically by a low concentration of Ca2+ and/or an intracellular ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fny097

    authors: Belevich G,Belevich N,Knuuti J,Verkhovskaya M

    更新日期:2018-06-01 00:00:00

  • Sequencing and characterization of a novel serine metalloprotease from Burkholderia pseudomallei.

    abstract::Burkholderia pseudomallei, a Gram-negative bacterium is found in the soil and water, mainly in Southeast Asia and Northern Australia. It is responsible for melioidosis in human and animals. The bacteria produce several potential virulent factors such as extracellular protease, hemolysin, lipase and lecithinase. The is...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09360.x

    authors: Lee MA,Liu Y

    更新日期:2000-11-01 00:00:00

  • A novel type-III staphylococcal cassette chromosome mec (SCCmec) variant among Indian isolates of methicillin-resistant Staphylococcus aureus.

    abstract::We identified a novel type-III staphylococcal cassette chromosome mec (SCCmec) element carried by eight methicillin-resistant Staphylococcus aureus (MRSA) strains from different wards and patients in an Indian hospital. Although the pulsed-field gel electrophoresis pattern and spa types of eight strains were identical...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01482.x

    authors: Arakere G,Nadig S,Ito T,Ma XX,Hiramatsu K

    更新日期:2009-03-01 00:00:00

  • Spreading the message of antimicrobial resistance: a detailed account of a successful public engagement event.

    abstract::The increase in Antimicrobial resistance (AMR) microorganisms has been exacerbated by exposure to antimicrobial drugs (e.g. antibiotics). A solution to AMR may require academic researchers to not only contribute to the drug discovery pipeline through laboratory research, but also to engage and inform non-specialist au...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fny175

    authors: Redfern J,Bowater L,Crossley M,Verran J

    更新日期:2018-08-01 00:00:00

  • PCR primers specific for the genus Tuber reveal the presence of several truffle species in a truffle-ground.

    abstract::Truffles are hypogeous Ascomycete fungi belonging to the genus Tuber and forming fruiting bodies highly prized for their taste and aroma. The identification of the genus Tuber and its species is important to investigate their ecology and avoid fraud in the food market. As genus-specific primers are not available, the ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01655.x

    authors: Zampieri E,Mello A,Bonfante P,Murat C

    更新日期:2009-08-01 00:00:00

  • Enhanced expression of aconitase raises acetic acid resistance in Acetobacter aceti.

    abstract::Acetobacter spp. are used for industrial vinegar production because of their high ability to oxidize ethanol to acetic acid and high resistance to acetic acid. Two-dimensional gel electrophoretic analysis of a soluble fraction of Acetobacter aceti revealed the presence of several proteins whose production was enhanced...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.05.007

    authors: Nakano S,Fukaya M,Horinouchi S

    更新日期:2004-06-15 00:00:00

  • Lysis of staphylococcal mastitis pathogens by bacteriophage phi11 endolysin.

    abstract::The Staphylococcus aureus bacteriophage phi11 endolysin has two peptidoglycan hydrolase domains (endopeptidase and amidase) and an SH3b cell wall-binding domain. In turbidity reduction assays, the purified protein can lyse untreated staphylococcal mastitis pathogens, Staphylococcus aureus and coagulase-negative staphy...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00483.x

    authors: Donovan DM,Lardeo M,Foster-Frey J

    更新日期:2006-12-01 00:00:00

  • Glanders: off to the races with Burkholderia mallei.

    abstract::Burkholderia mallei, the etiologic agent of the disease known as glanders, is primarily a disease affecting horses and is transmitted to humans by direct contact with infected animals. The use of B. mallei as a biological weapon has been reported and currently, there is no vaccine available for either humans or animal...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.2007.00949.x

    authors: Whitlock GC,Estes DM,Torres AG

    更新日期:2007-12-01 00:00:00

  • Impact of the small RNA RyhB on growth, physiology and heterologous protein expression in Escherichia coli.

    abstract::The small noncoding RNA RyhB is a regulator of iron homeostasis in Escherichia coli. During iron limitation, it downregulates the expression of a number of iron-containing proteins, including enzymes of the tricarboxylic acid cycle and the respiratory chain. Because this infers a potential for RyhB to limit energy met...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00880.x

    authors: Bollinger CJ,Kallio PT

    更新日期:2007-10-01 00:00:00

  • Roseovarius sp. strain 217: aerobic taurine dissimilation via acetate kinase and acetate-CoA ligase.

    abstract::The genome sequence of Roseovarius sp. strain 217 indicated that many pathway enzymes found in other organisms for the degradation of taurine are represented, but that a novel, apparently energy-dependent pathway is involved in the conversion of acetyl phosphate to acetyl CoA. Thus, an ABC transporter for taurine coul...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00719.x

    authors: Baldock MI,Denger K,Smits TH,Cook AM

    更新日期:2007-06-01 00:00:00

  • Cloning and characterization of genes encoding an enzyme which oxidizes dimethyl sulfide in Acinetobacter sp. strain 20B.

    abstract::Acinetobacter sp. strain 20B was isolated based on the ability to utilize dimethyl sulfide as the sole sulfur source. Since strain 20B oxidized indole as well as dimethyl sulfide, indigo production by recombinant Escherichia coli clones carrying Acinetobacter DNA was used as a selection for cloning genes encoding dime...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb12692.x

    authors: Horinouchi M,Kasuga K,Nojiri H,Yamane H,Omori T

    更新日期:1997-10-01 00:00:00

  • Knockout of the alanine racemase gene in Aeromonas hydrophila HBNUAh01 results in cell wall damage and enhanced membrane permeability.

    abstract::This study focused on the alanine racemase gene (alr-2), which is involved in the synthesis of d-alanine that forms the backbone of the cell wall. A stable alr-2 knockout mutant of Aeromonas hydrophila HBNUAh01 was constructed. When the mutant was supplemented with d-alanine, growth was unaffected; deprivation of d-al...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv089

    authors: Liu D,Zhang L,Xue W,Wang Y,Ju J,Zhao B

    更新日期:2015-07-01 00:00:00

  • Genomic 3' terminal sequence comparison of three isolates of rabbit haemorrhagic disease virus.

    abstract::Comparison of sequence data is necessary in older to investigate virus origins, identify features common to virulent strains, and characterize genomic organization within virus families. A virulent caliciviral disease of rabbits recently emerged in China. We have sequenced 1100 bases from the 3' ends of two independen...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90486-8

    authors: Milton ID,Vlasak R,Nowotny N,Rodak L,Carter MJ

    更新日期:1992-05-15 00:00:00

  • The antibacterial effects of terpene alcohols on Staphylococcus aureus and their mode of action.

    abstract::The study was made of the antibacterial effects of three terpene alcohols on Staphylococcus aureus, focusing on the leakage of K+ ions and toxicity over time. The leakage of K+ ions was monitored continuously with a K+-electrode. Our results suggested that the terpene alcohols, namely, farnesol, nerolidol and plaunoto...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.06.049

    authors: Inoue Y,Shiraishi A,Hada T,Hirose K,Hamashima H,Shimada J

    更新日期:2004-08-15 00:00:00

  • Transcription and genetic analyses of a putative N-acetylmuramyl-L-alanine amidase in Borrelia burgdorferi.

    abstract::In this study, a putative N-acetylmuramyl-l-alanine amidase gene (bb0666) was identified in the genome of the Lyme disease spirochete Borrelia burgdorferi. This protein shares c. 30% identity with its counterparts from other bacteria. Reverse transcriptase-PCR analysis showed that bb0666 along with two other genes (bb...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01416.x

    authors: Yang Y,Li C

    更新日期:2009-01-01 00:00:00

  • A new pullulan and a branched (1-->3)-, (1-->6)-linked beta-glucan from the lichenised ascomycete Teloschistes flavicans.

    abstract::The polysaccharides formed on hot alkaline extraction of the ascomycetous lichen Teloschistes flavicans were fractionated to give two glucans, which were characterised by methylation analysis and 1D and 2D NMR spectroscopy. One was a branched beta-glucan containing (1-->3) and (1-->6) linkages, a structure which is mo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11152.x

    authors: Reis RA,Tischer CA,Gorin PA,Iacomini M

    更新日期:2002-04-23 00:00:00

  • Recent advances in development of cell-free protein synthesis systems for fast and efficient production of recombinant proteins.

    abstract::Cell-free protein synthesis has emerged in recent years as a powerful tool that can potentially transform the production of recombinant proteins. Cell-free protein synthesis harnesses the synthetic power of living cells while eliminating many of the constraints of traditional cell-based gene expression methods. Due to...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fny174

    authors: Lee KH,Kim DM

    更新日期:2018-09-01 00:00:00

  • Non-radioactive restriction fragment length polymorphism (RFLP) typing of Clostridium difficile.

    abstract::A typing method for Clostridium difficile based on restriction fragment length polymorphisms (RFLP) is described. The technique utilizes commercially available Escherichia coli ribosomal ribonucleic acid (rRNA) as probe material. Probe labelling, hybridization and detection was performed using the Enhanced Chemilumine...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(91)90097-t

    authors: Bowman RA,O'Neill GL,Riley TV

    更新日期:1991-04-15 00:00:00

  • The oxyR gene from Erwinia carotovora: cloning, sequence analysis and expression in Escherichia coli.

    abstract::Homologs of the Escherichia coli oxyR gene were identified in several Erwinia species, using a combination of PCR and Southern hybridization analysis. The oxyR gene from Erwinia carotovora was isolated on a cosmid clone and characterized. The gene and deduced gene product shared high level sequence identity with their...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13242.x

    authors: Calcutt MJ,Lewis MS,Eisenstark A

    更新日期:1998-10-15 00:00:00

  • In vitro evaluation of the prebiotic properties of almond skins (Amygdalus communis L.).

    abstract::In this study we investigated the potential prebiotic effect of natural (NS) and blanched (BS) almond skins, the latter being a byproduct of the almond-processing industry. A full model of the gastrointestinal tract, including in vitro gastric and duodenal digestion, followed by colonic fermentation using mixed faecal...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.01898.x

    authors: Mandalari G,Faulks RM,Bisignano C,Waldron KW,Narbad A,Wickham MS

    更新日期:2010-03-01 00:00:00

  • A chemiluminescence immunoassay for evaluation of Cryptosporidium parvum growth in vitro.

    abstract::A chemiluminescence immunoassay (CLIA) was developed to detect Cryptosporidium parvum growth in Madin-Darby canine kidney (MDCK) cell cultures. Optimal results were obtained when MDCK cells were plated at a density of 1 x 10(4) cells/well (96-well plate) and maintained as a monolayer for 4 days prior to infection with...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08057.x

    authors: You X,Arrowood MJ,Lejkowski M,Xie L,Schinazi RF,Mead JR

    更新日期:1996-03-01 00:00:00

  • Conformational plasticity of molecular chaperones involved in periplasmic and outer membrane protein folding.

    abstract::How proteins reach their native conformation and location has been a major question of biology during the last 50 years. To counterbalance protein misfolding and the accumulation of aggregation products, a complex network of chaperones and proteases takes care of protein quality control in the cell. Such a chaperone n...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fny121

    authors: Mas G,Hiller S

    更新日期:2018-07-01 00:00:00

  • Horizontal gene transfer of chromosomal Type II toxin-antitoxin systems of Escherichia coli.

    abstract::Type II toxin-antitoxin systems (TAs) are small autoregulated bicistronic operons that encode a toxin protein with the potential to inhibit metabolic processes and an antitoxin protein to neutralize the toxin. Most of the bacterial genomes encode multiple TAs. However, the diversity and accumulation of TAs on bacteria...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv238

    authors: Ramisetty BC,Santhosh RS

    更新日期:2016-02-01 00:00:00

  • Infection by the 'photosynthetic' phage S-PM2 induces increased synthesis of phycoerythrin in Synechococcus sp. WH7803.

    abstract::Phycoerythrin-containing Synechococcus strains are unicellular cyanobacteria that are of great ecological importance in the marine environment. These organisms are known to be susceptible to infection by cyanophages (viruses that infect cyanobacteria). The infection cycle takes several hours and during this time the c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01148.x

    authors: Shan J,Jia Y,Clokie MR,Mann NH

    更新日期:2008-06-01 00:00:00

  • Marinitalea sucinacia gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from tidal flat sediment.

    abstract::An amber-pigmented, Gram-negative, rod-shaped and aerobic bacterial strain devoid of flagella, designated strain JC2131(T) , was isolated from tidal flat sediment of Dongmak in Ganghwa island, South Korea. Identification was carried out on the basis of polyphasic taxonomy. Phylogenetic analysis based on the 16S rRNA g...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.02147.x

    authors: Kim KY,Park SJ,Hahm YT,Cha CJ

    更新日期:2011-01-01 00:00:00

  • System-level modeling of acetone-butanol-ethanol fermentation.

    abstract::Acetone-butanol-ethanol (ABE) fermentation is a metabolic process of clostridia that produces bio-based solvents including butanol. It is enabled by an underlying metabolic reaction network and modulated by cellular gene regulation and environmental cues. Mathematical modeling has served as a valuable strategy to faci...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fnw074

    authors: Liao C,Seo SO,Lu T

    更新日期:2016-05-01 00:00:00

  • Detection of Nitrosomonas spp. by polymerase chain reaction.

    abstract::A unique genomic DNA fragment was isolated from Nitrosomonas europaea ATCC 19718. Based on the sequence of this fragment, oligonucleotide primers for polymerase chain reaction amplification were prepared which amplify sequences of 775 and 658 bp. The predicted DNA fragments were both amplified from the genome of N. eu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb07029.x

    authors: Nejidat A,Abeliovich A

    更新日期:1994-07-01 00:00:00

  • Surface Listeria monocytogenes carbohydrate-binding components revealed by agglutination with neoglycoproteins.

    abstract::Carbohydrate-binding components were shown to be present at the surface of Listeria monocytogenes by means of a panel of neoglycoproteins using direct agglutination. These lectin-like components bind on neoglycoproteins bearing D-glucosamine, L-fucosylamine, or para-amino-phenyl-alpha-D-mannopyrannoside residues. The ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/s0378-1097(05)80058-8

    authors: Cottin J,Loiseau O,Robert R,Mahaza C,Carbonnelle B,Senet JM

    更新日期:1990-03-15 00:00:00