Bioplastic reservoir of diverse bacterial communities revealed along altitude gradient of Pangi-Chamba trans-Himalayan region.

Abstract:

:Himalaya hosts a unique environment for microbial ecology. The present study aimed to explore the bioplastic producing bacterial communities along altitude gradient of Pangi-Chamba trans-Himalayan region. A total of 411 bacteria were isolated and 70 characterized at the molecular level for potential polyhydroxyalkanoates (PHA) producers. The most abundant phylum for PHA synthesis was Proteobacteria (73%), followed by Actinobacteria (11%), Firmicutes (10%) and Bacteroidetes (6%). However, at the genus level, Pseudomonas and Janthinobacterium were dominantly reported. Also, the ability to synthesize PHA was reported for the first time for few genera such as Collimonas, Pseudarthrobacter and Paenarthrobacter. Phylogenetic analysis of partial 16S rDNA and phaC genes revealed conservation in phaC and possibility of horizontal gene transfer among distant taxa. Furthermore, GC-MS also confirmed the ability of potential bacterial isolates to synthesize PHA. In fact, we found that PHA-positive bacteria are dominant in the high altitude of Himalaya, suggesting the vital role of PHA in bacterial adaptation and survival. Together, these findings had revealed the rich bacterial diversity and genetic machinery for PHA synthesis which does have potential for further utilization in the commercial applications.

journal_name

FEMS Microbiol Lett

authors

Kumar V,Thakur V,Ambika,Kumar S,Singh D

doi

10.1093/femsle/fny144

subject

Has Abstract

pub_date

2018-07-01 00:00:00

issue

14

eissn

0378-1097

issn

1574-6968

pii

5037922

journal_volume

365

pub_type

杂志文章
  • Prebiotics and gut microbiota in chickens.

    abstract::Prebiotics are non-digestible feed ingredients that are metabolized by specific members of intestinal microbiota and provide health benefits for the host. Fermentable oligosaccharides are best known prebiotics that have received increasing attention in poultry production. They act through diverse mechanisms, such as p...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fnv122

    authors: Pourabedin M,Zhao X

    更新日期:2015-08-01 00:00:00

  • Molecular monitoring and characterization of the faecal microbiota of healthy dogs during fructan supplementation.

    abstract::The large intestine of dogs contains a complex microbial ecosystem with predominance of streptococci, bifidobacteria, lactobacilli, Bacteroides and Clostridium. Generally, this predominant microbiota in dogs is relatively stable in time but much less is known about its taxonomic composition. Moreover, almost no studie...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.06.003

    authors: Vanhoutte T,Huys G,De Brandt E,Fahey GC Jr,Swings J

    更新日期:2005-08-01 00:00:00

  • Draft genome sequence of the Chilean isolate Aeromonas salmonicida strain CBA100.

    abstract::We report the draft genome sequence from Aeromonas salmonicida sp. strain CBA100, which was characterized as an antibiotic-resistant bacterium isolated from infected rainbow trout. The total size of the genome is 4,788,109 bp, with a G + C content of 60.55%. Comparison of its open reading frames shows that the closest...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnu062

    authors: Valdes N,Espinoza C,Sanhueza L,Gonzalez A,Corsini G,Tello M

    更新日期:2015-03-01 00:00:00

  • Description of Sulfitobacter sediminilitoris sp. nov., isolated from a tidal flat.

    abstract::A Gram-stain-negative bacterial strain, JBTF-M27T, was isolated from a tidal flat from Yellow Sea, Republic of Korea. Neighbor-joining phylogenetic tree of 16S rRNA gene sequences showed that strain JBTF-M27T fell within the clade comprising the type strains of Sulfitobacter species. Strain JBTF-M27T exhibited the hig...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnaa074

    authors: Park S,Chen S,Lee JS,Kim W,Yoon JH

    更新日期:2020-05-01 00:00:00

  • Haemin binding as a factor in the virulence of Porphyromonas gingivalis.

    abstract::Haemin (iron protoporphyrin IX) is an essential growth factor for the periodontal pathogen. Porphyromonas gingivalis. Iron protoporphyrin IX (IPP IX) binding to the avirulent P. gingivalis beige variant (W50/BE1) and the black-pigmenting parent wild-type strain W50 was quantified. W50/BE1 grown in a chemostat under ha...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08364.x

    authors: Smalley JW,Birss AJ,McKee AS,Marsh PD

    更新日期:1996-07-15 00:00:00

  • A European questionnaire-based study on population awareness and risk perception of antimicrobial resistance.

    abstract::To tackle antimicrobial resistance (AMR) is of outmost importance for the general population to understand the severity and the relevance of different routes of transmission. Respondents of different age groups, educational and occupational backgrounds, area of living, diet and household composition participated in an...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz221

    authors: Alexa Oniciuc EA,Likotrafiti E,Garre A,Ruiz L,Prieto M,Alvarez-Ordóñez A

    更新日期:2019-09-01 00:00:00

  • Expression and purification of Clostridium perfringens beta-toxin glutathione S-transferase fusion protein.

    abstract::The beta-toxin gene from Clostridium perfringens type C was cloned and expressed as a glutathione S-transferase fusion protein in Escherichia coli. The DNA sequence was determined and compared to the type B sequence. Two nucleotide differences were found in the protein coding sequence, resulting in one amino acid diff...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07731.x

    authors: Steinthorsdottir V,Fridriksdottir V,Gunnarsson E,Andrésson OS

    更新日期:1995-08-01 00:00:00

  • Conformational plasticity of molecular chaperones involved in periplasmic and outer membrane protein folding.

    abstract::How proteins reach their native conformation and location has been a major question of biology during the last 50 years. To counterbalance protein misfolding and the accumulation of aggregation products, a complex network of chaperones and proteases takes care of protein quality control in the cell. Such a chaperone n...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fny121

    authors: Mas G,Hiller S

    更新日期:2018-07-01 00:00:00

  • Characterization of a recombinant transferrin-binding protein A (TbpA) fragment from Haemophilus parasuis serovar 5.

    abstract::Haemophilus parasuis, the etiological agent of Glässer's disease in pigs, possesses iron acquisition pathways mediated by a surface receptor that specifically bind porcine transferrin. This receptor is composed of transferrin-binding protein A (TbpA) and TbpB. As it has been reported for other gram-negative organisms,...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.01970.x

    authors: Martínez S,Frandoloso R,Rodríguez-Ferri EF,González-Zorn B,Gutiérrez-Martín CB

    更新日期:2010-06-01 00:00:00

  • Genetic engineering of Streptococcus gordonii for the simultaneous display of two heterologous proteins at the bacterial surface.

    abstract::The Gram-positive bacterium Streptococcus gordonii has been genetically engineered to allow the simultaneous expression of two heterologous proteins at the cell surface. A family of recombinant streptococci displaying two different antigens was constructed. All the strains were genetically stable and expressed both pr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11172.x

    authors: Maggi T,Spinosa M,Ricci S,Medaglini D,Pozzi G,Oggioni MR

    更新日期:2002-04-23 00:00:00

  • Accumulation of ppGpp in Streptococcus pyogenes and Streptococcus rattus following amino acid starvation.

    abstract::We have re-examined the stringent response of Streptococcus rattus and Streptococcus pyogenes, two organisms that had originally been reported not to accumulate ppGpp following amino acid deprivation. We conclude that ppGpp does accumulate when S. rattus and S. pyogenes are deprived of isoleucine by mupirocin addition...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb12836.x

    authors: Whitehead KE,Webber GM,England RR

    更新日期:1998-02-01 00:00:00

  • Comparison of the thickening properties of four Lactobacillus delbrueckii subsp. bulgaricus strains and physicochemical characterization of their exopolysaccharides.

    abstract::It is now well established that physicochemical properties of exopolysaccharides (EPS) can vary between strains of a given species and according to growth conditions. The EPS production of four strains of Lactobacillus bulgaricus was monitored during growth in milk and in a chemically defined media. All strains, inclu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00214-3

    authors: Petry S,Furlan S,Waghorne E,Saulnier L,Cerning dagger J,Maguin E

    更新日期:2003-04-25 00:00:00

  • Diatom DNA barcodes for forensic discrimination of drowning incidents.

    abstract::The presence of diatoms in victim's internal organs has been regarded as a gold biological evidence of drowning. The idea becomes true at the advent of DNA metabarcoding. Unfortunately, the DNA barcode of diatoms are far from being applicable due to neither consensus on the barcode and nor reliable reference library.I...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnaa145

    authors: Liu M,Zhao Y,Sun Y,Wu P,Zhou S,Ren L

    更新日期:2020-09-01 00:00:00

  • Arthrospira ('Spirulina') strains from four continents are resolved into only two clusters, based on amplified ribosomal DNA restriction analysis of the internally transcribed spacer.

    abstract::We present the results of a phylogenetic study, based on amplified ribosomal DNA restriction analysis of the rDNA operon, of 37 Arthrospira ('Spirulina') cultivated clonal strains from four continents. In addition, duplicates from different culture collections or markedly different morphotypes of particular strains es...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13471.x

    authors: Scheldeman P,Baurain D,Bouhy R,Scott M,Mühling M,Whitton BA,Belay A,Wilmotte A

    更新日期:1999-03-15 00:00:00

  • Conjugational cotransfer of IncFI and IncI conjugative plasmids forming aggregate in a pathogenic Citrobacter freundii strain.

    abstract::Two plasmids, pEM4 (IncFI) determining virulence and pEM6 (IncI) determining colicine I production and resistance to tetracycline, have been found in a pathogenic strain of Citrobacter freundii. Even though pEM4 and pEM6 plasmids are conjugative and transfer themselves very efficiently during conjugation, a high cotra...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90115-7

    authors: Morzejko E,Panek E,Cebrat S

    更新日期:1990-03-01 00:00:00

  • Mouse pathogenicity studies of Nocardia asteroides complex species and clinical correlation with human isolates.

    abstract::Nocardia asteroides complex organisms derived from human specimens between 1979 and 1992 were identified to the species level. Of 117 N. asteroides complex organisms, 34 (29%) were N. farcinica, 28 (24%) were N. nova, and 55 (47%) were N. asteroides sensu stricto. An analysis of the specimen sites from which the organ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06335.x

    authors: Desmond EP,Flores M

    更新日期:1993-07-01 00:00:00

  • Salicylate uniquely induces a 27-kDa protein in tubercle bacillus.

    abstract::Salicylate was found to uniquely induce a 27-kDa protein in Mycobacterium tuberculosis complex organisms but not in Mycobacterium smegmatis or Escherichia coli. The structural analogue antitubercular para-amino-salicylate also induced the 27-kDa protein but to a somewhat lower level than salicylate. Other structural a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10843.x

    authors: Sun Z,Cheng SJ,Zhang H,Zhang Y

    更新日期:2001-09-25 00:00:00

  • Purification and properties of D-(-)-3-hydroxybutyrate oligomer hydrolase of Paracoccus denitrificans.

    abstract::D-(-)-3-Hydroxybutyrate (3HB) oligomer hydrolase was purified from Paracoccus denitrificans. The enzyme was a monomeric protein with an approximate molecular mass of 31 kDa. The isoelectric point of the enzyme was 5.2. Optimum temperature and pH were 35-40 degrees C and 8.0, respectively. The enzyme activity was not a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11006.x

    authors: Ueda S,Sano K,Gao D,Tomihari N,Yamane T,Endo I

    更新日期:2002-01-10 00:00:00

  • Virulence determinants in enteroaggregative Escherichia coli from North India and their interaction in in vitro organ culture system.

    abstract::Enteroaggregative Escherichia coli (EAEC) is an important diarrhoeal pathogen causing diseases in multiple epidemiological and clinical settings. In developing countries like India, diarrhoeal diseases are one of the major killers among paediatric population and oddly, few studies are available from Indian paediatric ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw189

    authors: Gupta D,Sharma M,Sarkar S,Thapa BR,Chakraborti A

    更新日期:2016-09-01 00:00:00

  • Low heat resistance of Bacillus sphaericus spores correlated with high protoplast water content.

    abstract::The low heat resistance (D100 = 0.554 min, z = 13.4 degrees C) of dormant lysozyme-sensitized spores of Bacillus sphaericus 9602 was correlated with a low protoplast wet density (1.305 g/ml) equivalent to a high protoplast water content (61.0%, wet weight basis). These values for these unusual spores were consistent w...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(89)90331-5

    authors: Beaman TC,Pankratz HS,Gerhardt P

    更新日期:1989-03-01 00:00:00

  • H4 histone in the macronucleus of Blepharisma japonicum (Protozoa, Ciliophora, Heterotrichida).

    abstract::Two clones, obtained by polymerase chain reaction from macronuclear DNA of the unicellular ciliated protist Blepharisma japonicum, were isolated and sequenced. They correspond to fragments of two different putative H4 histone genes. The existence of multiple H4 histone genes was also suggested by Southern blot hybridi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb10314.x

    authors: Salvini M,Bini E,Santucci A,Batistoni R

    更新日期:1997-04-01 00:00:00

  • Efflux of choline and glycine betaine from osmoregulating cells of Escherichia coli.

    abstract::We present evidence that glycine betaine (betaine) which was synthesized from choline was excreted and reaccumulated in osmoregulating cells of Escherichia coli. Choline which was accumulated in bet mutants defective in betaine synthesis was shown to be excreted in response to betaine uptake. Our data suggest that E. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90395-5

    authors: Lamark T,Styrvold OB,Strøm AR

    更新日期:1992-09-15 00:00:00

  • Bacillus thuringiensis serovar israelensis is highly toxic to the coffee berry borer, Hypothenemus hampei Ferr. (Coleoptera: Scolytidae).

    abstract::A native collection of Bacillus thuringiensis strains was screened, once a reliable bioassay technique to assess the toxicity against the coffee berry borer (CBB) first-instar larvae was developed. A first round of bioassays with 170 strains indicated that the great majority of them showed no or very little insecticid...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00557-3

    authors: Méndez-López I,Basurto-Ríos R,Ibarra JE

    更新日期:2003-09-12 00:00:00

  • Teaching matters-academic professional development in the early 21st century.

    abstract::Academic work at different career stages has changed and a broadened portfolio of expertise enables academics to adapt, maintain and advance their career. Development related to research activity is naturally driven by methodology and technology. Institutions and peers largely support development in the contexts of di...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fnv156

    authors: Fahnert B

    更新日期:2015-10-01 00:00:00

  • Histidine aminotransferase activity in Streptomyces tendae and its correlation with nikkomycin production.

    abstract::Streptomyces tendae Tü901 produces nikkomycins belonging to the nucleoside peptide antibiotics. Mutants defective in histidine catabolism were isolated and characterized with regard to their histidine ammonium-lyase activity and antibiotic synthesis. In the histidine ammonialyase-negative mutant hut-11 which was unimp...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90384-z

    authors: Roos U,Mattern S,Schrempf H,Bormann C

    更新日期:1992-10-01 00:00:00

  • Listeria monocytogenes infection of Caco-2 human epithelial cells induces activation of transcription factor NF-kappa B/Rel-like DNA binding activities.

    abstract::The effect of Listeria monocytogenes infection on the cellular level of transcription factor NF-kappa B in the human epithelia-like cell line Caco-2 was investigated. Infection with L. monocytogenes or treatment with lipoteichoic acid induced the formation of three NF-kappa B-like DNA-protein complexes C1, C2, and C3,...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13766.x

    authors: Hauf N,Goebel W,Fiedler F,Kuhn M

    更新日期:1999-09-01 00:00:00

  • Microbial organic acid production as carbon dioxide sink.

    abstract::Mixed-substrate conversions are an under-regarded option to fix carbon dioxide in significant amounts. In such a conversion, carbon dioxide together with one other carbon source such as glucose is converted to a single carbon product. With mixed-substrate conversions, it is possible to incorporate carbon dioxide into ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx212

    authors: Steiger MG,Mattanovich D,Sauer M

    更新日期:2017-11-15 00:00:00

  • Sequencing and characterization of a novel serine metalloprotease from Burkholderia pseudomallei.

    abstract::Burkholderia pseudomallei, a Gram-negative bacterium is found in the soil and water, mainly in Southeast Asia and Northern Australia. It is responsible for melioidosis in human and animals. The bacteria produce several potential virulent factors such as extracellular protease, hemolysin, lipase and lecithinase. The is...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09360.x

    authors: Lee MA,Liu Y

    更新日期:2000-11-01 00:00:00

  • Construction of mini-Tn10luxABcam/Ptac-ATS and its use for developing a bacteriophage that transduces bioluminescence to Escherichia coli O157:H7.

    abstract::Mini-Tn10luxABcam/Ptac-ATS was constructed in order to develop a luciferase-transducing bacteriophage for detecting Escherichia coli O157:H7. The transposon was designed to deliver a 3.6-kb insertion that confers n-decanal-dependent bioluminescence and resistance to chloramphenicol and was constructed using mini-Tn10c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb08909.x

    authors: Waddell TE,Poppe C

    更新日期:2000-01-15 00:00:00

  • The immune response to Lactococcus lactis: implications for its use as a vaccine delivery vehicle.

    abstract::The development of natural antibodies to Lactococcus lactis in three inbred strains of mice and the effect of inoculating L. lactis into these mice has been investigated. All animals developed detectable levels of natural (systemic and secretory) anti-lactococcal antibodies. Systemic anti-lactococcal antibodies were p...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb07041.x

    authors: Norton PM,Brown HW,Le Page RW

    更新日期:1994-07-15 00:00:00