Comparison of the thickening properties of four Lactobacillus delbrueckii subsp. bulgaricus strains and physicochemical characterization of their exopolysaccharides.

Abstract:

:It is now well established that physicochemical properties of exopolysaccharides (EPS) can vary between strains of a given species and according to growth conditions. The EPS production of four strains of Lactobacillus bulgaricus was monitored during growth in milk and in a chemically defined media. All strains, including the non-ropy one, produced EPS. The monosaccharide composition, molar mass (M(w)), and intrinsic viscosity of these EPS were determined and compared. Further characterization using high-performance size-exclusion chromatography revealed the presence of two fractions in all EPS: one fraction exhibited a high M(w) and a high intrinsic viscosity while the other had a low M(w) and a low intrinsic viscosity. Strikingly, the EPS synthesized by the non-ropy strain was mainly composed of the low-M(w) fraction while for the ropy strains, the fraction of high M(w) varied between 43 and 90%. According to our results, we propose that the ratio between the high-M(w) and low-M(w) fractions is critical for the texturing properties of L. bulgaricus EPS.

journal_name

FEMS Microbiol Lett

authors

Petry S,Furlan S,Waghorne E,Saulnier L,Cerning dagger J,Maguin E

doi

10.1016/S0378-1097(03)00214-3

keywords:

subject

Has Abstract

pub_date

2003-04-25 00:00:00

pages

285-91

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378109703002143

journal_volume

221

pub_type

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