Teaching matters-academic professional development in the early 21st century.

abstract：:A novel strain of Rhizopus oryzae WPG secretes a noninduced lipase (ROLw) in the culture medium; purified ROLw is a protein of 29 kDa, the 45 N-terminal amino acid residues were sequenced, this sequence is very homologous to Rhizopus delemar lipase (RDL), Rhizopus niveus lipase (RNL) and R. oryzae lipase (ROL29) seque...

journal_title：FEMS microbiology letters

authors： Salah RB,Mosbah H,Fendri A,Gargouri A,Gargouri Y,Mejdoub H

更新日期：2006-07-01 00:00:00

abstract：:CRISPR-Cas adaptive immune systems protect bacteria from phage predation, and other foreign genetic elements such as plasmids. Significant advances have been made regarding how CRISPR-Cas systems elicit immunity; however, comparatively little is known about their regulation. To study CRISPR-Cas regulation, we describe...

journal_title：FEMS microbiology letters

authors： Hampton HG,Patterson AG,Chang JT,Taylor C,Fineran PC

更新日期：2019-06-01 00:00:00

abstract：:A DNA segment located immediately upstream of the eaeA gene of enterohemorrhagic Escherichia coli O157:H7 strain HA1 was cloned and sequenced. This segment contained an open reading frame encoding a predicted protein of 156 amino acids. A database search identified similar open reading frames upstream of the eaeA gene...

journal_title：FEMS microbiology letters

authors： Zhao S,Mitchell SE,Meng J,Doyle MP,Kresovich S

更新日期：1995-11-01 00:00:00

abstract：:The isolate NJ-101 obtained from agricultural soil was characterized and presumptively identified as Pseudomonas sp. The isolate exhibited efficient degradation of the insecticide carbofuran with a rate constant of 0.035 day(-1), following first-order rate kinetics. The ability of performing multifarious biological ac...

journal_title：FEMS microbiology letters

authors： Bano N,Musarrat J

更新日期：2004-02-09 00:00:00

abstract：:The objective of this work was to analyze the effects of thermosonication (TS) on induction of a viable but non-culturable (VBNC) state in Salmonella Typhimurium and to examine incubation factors affecting subsequent resuscitation of cells. A TS treatment of 380 W at 53°C for 30 min induced the VBNC state in S. Typhim...

journal_title：FEMS microbiology letters

authors： Liao H,Jiang L,Zhang R

更新日期：2018-02-01 00:00:00

abstract：:Small plasmids were found in two clinical isolates of Streptococcus pneumoniae from Spain (strains 671 and 678) and in one strain (SpR) isolated in Germany. All three strains contained one plasmid (2.75 to 3.1 kb) which is related to the only previously described pneumococcal plasmid, pDP1. Strains 678 and SpR carried...

journal_title：FEMS microbiology letters

authors： Sibold C,Markiewicz Z,Latorre C,Hakenbeck R

更新日期：1991-01-01 00:00:00

abstract：:Immunoassays are increasingly used to investigate the production, properties and fates of the cyanobacterial hepatotoxic microcystins in vitro and in vivo. Responses of an ELISA immunoassay to microcystins have been determined using the authentic toxin antigen, microcystin-LR, and conjugation products between the toxi...

journal_title：FEMS microbiology letters

authors： Metcalf JS,Beattie KA,Pflugmacher S,Codd GA

更新日期：2000-08-15 00:00:00

abstract：:Proteins which are secreted or associated with the cell envelope of Mycobacterium tuberculosis may contain protective T-cell epitopes. Prior to this study, a recombinant clone bank of enzymatically active M. tuberculosis-alkaline phosphatase fusions, were screened for immunogenicity in a murine T-cell model. Five of t...

journal_title：FEMS microbiology letters

authors： Moran AJ,Doran JL,Wu J,Treit JD,Ekpo P,Kerr VJ,Roberts AD,Orme IM,Galant S,Ress SR,Nano FE

更新日期：1999-08-01 00:00:00

abstract：:Salmonella enterica serovar Enteritidis (S. Enteritidis) possesses plasmids of different sizes and roles. Besides the serovar-specific virulence plasmid present in most field strains, S. Enteritidis can harbour plasmids of low molecular mass whose biological role is poorly understood. We therefore sequenced plasmid pC...

journal_title：FEMS microbiology letters

authors： Gregorova D,Pravcova M,Karpiskova R,Rychlik I

更新日期：2002-09-10 00:00:00

abstract：:L-arginine has attracted a great deal of attention as an agent for refolding denatured proteins, and the mildness of its effects offer hope for a wide range of potential applications for this substance, including medicines with few side effects. We report that both L- and D-arginine inhibits Na+-driven flagellar motor...

journal_title：FEMS microbiology letters

authors： Atsumi T,Yoshimura F,Sugiyama S

更新日期：2006-03-01 00:00:00

abstract：:Pectinases are enzymes that catalyze pectin degradation. There is a global demand for pectinases because of their wide utility and catalytic efficiency. Optimization of the fermentation process to increase the pectolytic enzyme activity is generally practiced to lower process costs, but whether temperature influences ...

journal_title：FEMS microbiology letters

authors： Guan Y,Yin D,Du X,Ye X

更新日期：2018-12-01 00:00:00

abstract：:During bacterial infection, professional phagocytes are attracted to the site of infection, where they constitute a first line of host cell defense. Their function is to engulf and destroy the pathogens. Thus, bacteria must withstand the bactericidal activity of professional phagocytes, including macrophages to counte...

journal_title：FEMS microbiology letters

authors： Tran SL,Ramarao N

更新日期：2013-10-01 00:00:00

abstract：:DNA polymerase from the archaeon Pyrococcus abyssi strain Orsay was expressed in Escherichia coli. The recombinant DNA polymerase (Pab) was purified to homogeneity by heat treatment followed by 5 steps of chromatography and characterized for PCR applications. Buffer optimization experiments indicated that Pab PCR perf...

journal_title：FEMS microbiology letters

authors： Dietrich J,Schmitt P,Zieger M,Preve B,Rolland JL,Chaabihi H,Gueguen Y

更新日期：2002-11-19 00:00:00

abstract：:Dissimilatory metal-reducing bacteria (DMRB), such as Shewanella oneidensis MR-1, are of great interest for their importance in the biogeochemical cycling of metals and utility in biotechnological processes, such as bioremediation and microbial fuel cells. To identify genes necessary for metal reduction, this study co...

journal_title：FEMS microbiology letters

authors： Kouzuma A,Hashimoto K,Watanabe K

更新日期：2012-01-01 00:00:00

abstract：:The hepA gene in Anabaena sp. strain PCC 7120 is required for normal formation of the polysaccharide layer of the heterocyst envelope. A plasmid bearing hepA, interrupted by a neomycin-resistance cassette, was transferred by conjugation to wild type Anabaena variabilis ATCC 29413, so that the interrupted hepA gene rep...

journal_title：FEMS microbiology letters

authors： Leganés F

更新日期：1994-10-15 00:00:00

abstract：:A characteristic feature of fungal hypha is the presence of large number of nuclei in a common cytoplasmic environment. Where it has been examined, the coenocytic mycelium is commonly heterokaryotic. The nuclei cooperate, compete or combat. It is proposed that in addition to their classical role in heredity, supernume...

journal_title：FEMS microbiology letters

authors： Maheshwari R

更新日期：2005-08-01 00:00:00

abstract：:The chemical and molecular mechanism of toxicity of nitrite towards food-spoilage bacteria such as Clostridium botulinum or Clostridium sporogenes is not well understood. In order to discover the active species and explore its chemistry, a number of compounds related to nitrite were synthesized. Their bacteriocidal ef...

journal_title：FEMS microbiology letters

authors： Cui X,Joannou CL,Hughes MN,Cammack R

更新日期：1992-11-01 00:00:00

abstract：:Saccharomyces cerevisiae strains tolerant to osmotic stress are important for ethanol production during very high gravity (VHG) fermentation. We aimed to identify novel genes that confer enhanced tolerance to osmotic stress in S. cerevisiae. Two strains tolerant to up to 30% (w/v) glucose were isolated by screening a ...

journal_title：FEMS microbiology letters

authors： Kim B,Kim HS

更新日期：2018-07-01 00:00:00

abstract：:Hopanoid fingerprints allowed to differentiate bacteria formerly connected to the genus Pseudomonas. Whereas all strains related to Pseudomonas and Ralstonia were devoid of any detectable hopanoid, these pentacyclic triterpenoids were found in the Burkholderia species and in related soil isolates, which contained as m...

journal_title：FEMS microbiology letters

authors： Cvejic JH,Putra SR,El-Beltagy A,Hattori R,Hattori T,Rohmer M

更新日期：2000-02-15 00:00:00

abstract：:Antibody to Escherichia coli O157 lipopolysaccharide was detected in the sera of healthy individuals more frequently in Southern Thailand than in Japan. The result suggested possible exposure of Thai people to E. coli O157. E. coli O157:H7 or O157:H(-) was isolated from four of 95 retail beef and one of 55 bovine fece...

journal_title：FEMS microbiology letters

authors： Vuddhakul V,Patararungrong N,Pungrasamee P,Jitsurong S,Morigaki T,Asai N,Nishibuchi M

更新日期：2000-01-15 00:00:00

abstract：:In this study, internal size variations in the VanA gene cluster Tn1546, encoding resistance to glycopeptides, is described. Studies of previously uncharacterized size variations of an internal region, encoding the vanX and vanY genes of Tn1546, revealed that these variations were due to the presence of the IS sequenc...

journal_title：FEMS microbiology letters

authors： Jensen LB

更新日期：1998-12-15 00:00:00

abstract：:We developed a multiplex PCR to detect the presence of methicillin- (mecA), cadmium/zinc-(czrC) and antiseptic-resistant (qacA/B) staphylococci and to identify Panton-Valentine leukocidin (PVL)-positive and -negative Staphylococcus aureus and coagulase-negative staphylococci (CoNS) from infected and healthy eyes. The ...

journal_title：FEMS microbiology letters

authors： Panda S,Kar S,Choudhury R,Sharma S,Singh DV

更新日期：2014-03-01 00:00:00

abstract：:The plant antimicrobial peptide MiAMP1 from Macadamia integrifolia and the yeast killer toxin peptide WmKT from Williopsis mrakii are structural homologues. Comparative studies of yeast mutants were performed to test their sensitivity to these two antimicrobial peptides. No differences in susceptibility to MiAMP1 were...

journal_title：FEMS microbiology letters

authors： Stephens C,Kazan K,Goulter KC,Maclean DJ,Manners JM

更新日期：2005-02-01 00:00:00

abstract：:Arbuscular mycorrhizal fungi (AMF) belong to phylum Glomeromycota, an early divergent fungal lineage forming symbiosis with plant roots. Many reports have documented that bacteria are intimately associated with AMF mycelia in the soil. However, the role of these bacteria remains unclear and their diversity within intr...

journal_title：FEMS microbiology letters

authors： Iffis B,St-Arnaud M,Hijri M

更新日期：2014-09-01 00:00:00

abstract：:Gene disruption experiments play an important role in the functional characterization of genes in mycobacteria and rely mostly on the use of one or two antibiotic resistance markers. We have developed a system for mycobacteria which features both the advantages of the use of antibiotic resistance markers for gene disr...

journal_title：FEMS microbiology letters

authors： Malaga W,Perez E,Guilhot C

更新日期：2003-02-28 00:00:00

abstract：:Bacteriophage M102 is a virulent siphophage that propagates in some serotype c Streptococcus mutans strains, but not in S. mutans of serotype e, f or k. The serotype of S. mutans is determined by the glucose side chain of rhamnose-glucose polysaccharide (RGP). Because the first step in the bacteriophage infection proc...

journal_title：FEMS microbiology letters

authors： Shibata Y,Yamashita Y,van der Ploeg JR

更新日期：2009-05-01 00:00:00

abstract：:Most Moraxella catarrhalis isolates express a highly-conserved outer membrane protein of 453 residues designated OMPCD, which has been previously shown to mediate binding to A549 human lung cells. Here, it is reported that two distinct domains of the M. catarrhalis strain O35E OMPCD protein specify adherence. Truncate...

journal_title：FEMS microbiology letters

authors： Akimana C,Lafontaine ER

更新日期：2007-06-01 00:00:00

abstract：:The P39 antigen is a specific, highly conserved, and immunogenic protein of Lyme disease spirochetes, Borrelia burgdorferi sensu lato. The nucleotide sequence of the gene encoding this protein was determined and found to be the first of two tandemly arranged open reading frames located on the spirochete's chromosome. ...

journal_title：FEMS microbiology letters

authors： Simpson WJ,Cieplak W,Schrumpf ME,Barbour AG,Schwan TG

更新日期：1994-06-15 00:00:00

abstract：:An internal segment of the penicillin-binding protein gene, pbpA, of Streptomyces griseus was amplified from genomic DNA using the polymerase chain reaction and used as a hybridization probe to isolate the complete gene from a cosmid library. pbpA encodes a 485 amino acid sequence that conserves three motifs of PBPs, ...

journal_title：FEMS microbiology letters

authors： Jiang H,Kendrick KE

更新日期：2000-12-01 00:00:00

abstract：:Acinetobacter sp. strain 20B was isolated based on the ability to utilize dimethyl sulfide as the sole sulfur source. Since strain 20B oxidized indole as well as dimethyl sulfide, indigo production by recombinant Escherichia coli clones carrying Acinetobacter DNA was used as a selection for cloning genes encoding dime...

journal_title：FEMS microbiology letters

authors： Horinouchi M,Kasuga K,Nojiri H,Yamane H,Omori T

更新日期：1997-10-01 00:00:00