Molecular cloning of AbGst1 encoding a glutathione transferase differentially expressed during exposure of Alternaria brassicicola to isothiocyanates.

Abstract:

:The AbGst1 gene encoding a glutathione transferase from the necrotrophic pathogen Alternaria brassicicola was cloned from a benzyl isothiocyanate-treated conidial culture using differential display reverse transcription. The deduced amino-acid sequence of AbGst1p showed a significant degree of similarity to glutathione transferase-I from Saccharomyces cerevisiae and glutathione transferase-III from Schizosaccharomyces pombe. The transcription of AbGst1 was significantly enhanced by isothiocyanates, heavy metals and 1-chloro-2,4-dinitrobenzene. However, no significant transcript response was obtained with superoxide-generating menadione and paraquat. Recombinant AbGst1p expressed in Escherichia coli exhibited high transferase activity with allyl and benzyl isothiocyanates as substrate compared with 1-chloro-2,4-dinitrobenzene, but no peroxidase activity was detected. AbGst1 was upregulated in planta during the first day postinfection, suggesting the potential involvement of this enzyme in isothiocyanate detoxification mechanisms during host plant infection.

journal_name

FEMS Microbiol Lett

authors

Sellam A,Poupard P,Simoneau P

doi

10.1111/j.1574-6968.2006.00223.x

subject

Has Abstract

pub_date

2006-05-01 00:00:00

pages

241-9

issue

2

eissn

0378-1097

issn

1574-6968

pii

FML223

journal_volume

258

pub_type

杂志文章
  • Physical and genetic map of the Listeria monocytogenes EGD serotype 1/2a chromosome.

    abstract::Listeria monocytogenes is a facultative intracellular pathogen responsible for both invasive and non-invasive food-borne illness in animals and humans. In this study, macrorestriction analysis following pulsed-field gel electrophoresis was used to show that Listeria monocytogenes serovar 1/2a strain EGD has a single c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13632.x

    authors: von Both U,Otten S,Darbouche A,Domann E,Chakraborty T

    更新日期:1999-06-15 00:00:00

  • Phospholipids of Clostridium perfringens: a reexamination.

    abstract::We have identified phosphatidylethanolamine as one of the major phospholipids of Clostridium perfringens by two dimensional thin layer chromatography of the intact lipids and of their deacylation products and by liquid chromatography followed by mass spectrometry of the intact neutral phospholipid fraction. The princi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.01.048

    authors: Johnston NC,Baker JK,Goldfine H

    更新日期:2004-04-01 00:00:00

  • vpsA- and luxO-independent biofilms of Vibrio cholerae.

    abstract::The natural life cycle of Vibrio cholerae involves the transitioning of cells between different environmental surfaces such as the chitinous shell of Crustaceae and the epithelial layer of the human intestine. Previous studies using static biofilm systems showed a strict dependence of biofilm formation on the vps and ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00884.x

    authors: Müller J,Miller MC,Nielsen AT,Schoolnik GK,Spormann AM

    更新日期:2007-10-01 00:00:00

  • Taxonomic status of putative Verticillum alboatrum isolates.

    abstract::Two fungal isolates, formerly classified as Verticillium alboatrum and proposed as forming the basis of a new sub-group ('Group 2') within the species, have been shown to be non-pathogenic to known hosts of V. alboatrum and, on the basis of molecular evidence, to be closely related to Verticillium psalliotae and Verti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13376.x

    authors: Carder JH,Barbara DJ

    更新日期:1999-01-01 00:00:00

  • Identification of Mga1, a G-protein alpha-subunit gene involved in regulating citrinin and pigment production in Monascus ruber M7.

    abstract::The filamentous fungi Monascus spp., which have been used in traditional fermented food in Asia for centuries, are well-known producers of a group of bioactive metabolites that are widely used as food additives and nutraceutical supplements worldwide. However, its potential to produce the mycotoxin citrinin poses a th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.01992.x

    authors: Li L,Shao Y,Li Q,Yang S,Chen F

    更新日期:2010-07-01 00:00:00

  • Genotyping of clinical Serratia marcescens isolates: a comparison of PCR-based methods.

    abstract::The polymerase chain reaction (PCR)-based procedures of randomly amplified polymorphic DNA (RAPD) and repetitive element (RE)-based PCR were used to amplify total DNA prepared from each of 62 clinical Serratia marcescens isolates. Three different random primers, designated 1060, 1254 and 1283, were used individually i...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb09440.x

    authors: Patton TG,Katz S,Sobieski RJ,Crupper SS

    更新日期:2001-01-01 00:00:00

  • Diatom DNA barcodes for forensic discrimination of drowning incidents.

    abstract::The presence of diatoms in victim's internal organs has been regarded as a gold biological evidence of drowning. The idea becomes true at the advent of DNA metabarcoding. Unfortunately, the DNA barcode of diatoms are far from being applicable due to neither consensus on the barcode and nor reliable reference library.I...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnaa145

    authors: Liu M,Zhao Y,Sun Y,Wu P,Zhou S,Ren L

    更新日期:2020-09-01 00:00:00

  • Impact of land use history on the arbuscular mycorrhizal fungal diversity in arid soils of Argentinean farming fields.

    abstract::Arbuscular mycorrhizal fungi (AMF) are a key soil functional group, with an important potential to increase crop productivity and sustainable agriculture including food security. However, there is clear evidence that land uses, crop rotations and soil features affect the AMF diversity and their community functioning i...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnaa114

    authors: Ontivero RE,Voyron S,Allione LVR,Bianco P,Bianciotto V,Iriarte HJ,Lugo MA,Lumini E

    更新日期:2020-07-01 00:00:00

  • Ergosterol levels in two L-methionine-enriched mutants of the methylotrophic yeast Candida boidinii ICCF26.

    abstract::Two L-methionine-enriched mutants, SN-78 and SE-57, were isolated in a sulphur-deficient medium from the methylotrophic yeast Candida boidinii ICCF26. No significant differences were detected between the L-cysteine pools of the mutants and the wild-type. In mutant strain SE-57, S-adenosylmethionine and ergosterol leve...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90144-d

    authors: Avram D,Stan R

    更新日期:1992-11-01 00:00:00

  • Cloning and analysis of methanol oxidation genes in the methylotroph Hyphomicrobium methylovorum GM2.

    abstract::The gene encoding the alpha-subunit of methanol dehydrogenase (mxaF) and its flanking region was isolated from a methylotrophic bacterium, Hyphomicrobium methylovorum GM2. The deduced amino acid sequence of MxaF showed 80, 80, 74 and 66% identity with those of Methylobacterium extorquens AM1, M. organophilum XX, Parac...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb12673.x

    authors: Tanaka Y,Yoshida T,Watanabe K,Izumi Y,Mitsunaga T

    更新日期:1997-09-15 00:00:00

  • Characterization of DegU-dependent expression of bpr in Bacillus subtilis.

    abstract::The response regulator DegU and its cognate histidine kinase DegS constitute a two-component system in the Gram-positive soil bacterium Bacillus subtilis. The phosphorylated form of DegU is known to activate transcription of more than 120 genes in B. subtilis, including the bpr gene encoding bacillopeptidase F. To cha...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.01019.x

    authors: Tsukahara K,Ogura M

    更新日期:2008-03-01 00:00:00

  • Bioplastic reservoir of diverse bacterial communities revealed along altitude gradient of Pangi-Chamba trans-Himalayan region.

    abstract::Himalaya hosts a unique environment for microbial ecology. The present study aimed to explore the bioplastic producing bacterial communities along altitude gradient of Pangi-Chamba trans-Himalayan region. A total of 411 bacteria were isolated and 70 characterized at the molecular level for potential polyhydroxyalkanoa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fny144

    authors: Kumar V,Thakur V,Ambika,Kumar S,Singh D

    更新日期:2018-07-01 00:00:00

  • Industrial production of acetone and butanol by fermentation-100 years later.

    abstract::Microbial production of acetone and butanol was one of the first large-scale industrial fermentation processes of global importance. During the first part of the 20th century, it was indeed the second largest fermentation process, superseded in importance only by the ethanol fermentation. After a rapid decline after t...

    journal_title:FEMS microbiology letters

    pub_type: 历史文章,杂志文章

    doi:10.1093/femsle/fnw134

    authors: Sauer M

    更新日期:2016-07-01 00:00:00

  • Distinct DNA repair pathways involving RecA and nonhomologous end joining in Mycobacterium smegmatis.

    abstract::Mycobacterium smegmatis was used to study the relationship between DNA repair processes involving RecA and nonhomologous end joining (NHEJ). The effect of gene deletions in recA and/or in two genes involved in NHEJ (ku and ligD) was tested on the ability of bacteria to join breaks in plasmids transformed into them and...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00199.x

    authors: Korycka-Machala M,Brzostek A,Rozalska S,Rumijowska-Galewicz A,Dziedzic R,Bowater R,Dziadek J

    更新日期:2006-05-01 00:00:00

  • Identification of beta-oxidation and thioesterase activities in Staphylococcus carnosus 833 strain.

    abstract::Staphylococcus carnosus 833, inoculated into sausage meat, increased the level of methyl ketones, which contributed to the cured aroma. These ketones can arise from incomplete beta-oxidation followed by two enzymatic activities: a thioesterase and a decarboxylase. In this study we identified the beta-oxidative pathway...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09272.x

    authors: Engelvin G,Feron G,Perrin C,Mollé D,Talon R

    更新日期:2000-09-01 00:00:00

  • Test parameters and cell chain length of Streptococcus thermophilus affect the microbial adhesion to hydrocarbons assay: a methodical approach.

    abstract::The microbial adhesion to hydrocarbons (MATH) test is one of the most common method to determine the hydrophobicity of cell surfaces. Despite its prevalence, no standard test parameters are used in literature, making a direct comparison of data almost impossible. Criticism also focuses on test parameters that may mask...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz150

    authors: Nachtigall C,Weber C,Rothenburger S,Jaros D,Rohm H

    更新日期:2019-06-01 00:00:00

  • Genetic analysis of CTX prophages with special reference to ctxB and rstR alleles of Vibrio cholerae O139 strains isolated from Kolkata over a decade.

    abstract::Chronological analysis of 125 Vibrio cholerae O139 strains isolated during 1993-2005 in Kolkata revealed the prevalence of two new genotypes of cholera toxin (CT) and novel combinations of ctxB and rstR alleles resulting in variant CTX prophages. One of the new genotypes of ctxB, which first appeared in 1996 with the ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01856.x

    authors: Raychoudhuri A,Mukherjee P,Ramamurthy T,Nandy RK,Takeda Y,Nair GB,Mukhopadhyay AK

    更新日期:2010-02-01 00:00:00

  • Spiroethers of German chamomile inhibit production of aflatoxin G and trichothecene mycotoxin by inhibiting cytochrome P450 monooxygenases involved in their biosynthesis.

    abstract::The essential oil of German chamomile showed specific inhibition toward aflatoxin G(1) (AFG(1)) production, and (E)- and (Z)-spiroethers were isolated as the active compounds from the oil. The (E)- and (Z)-spiroethers inhibited AFG(1) production of Aspergillus parasiticus with inhibitory concentration 50% (IC(50)) val...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01195.x

    authors: Yoshinari T,Yaguchi A,Takahashi-Ando N,Kimura M,Takahashi H,Nakajima T,Sugita-Konishi Y,Nagasawa H,Sakuda S

    更新日期:2008-07-01 00:00:00

  • Sesquiterpene farnesol as a competitive inhibitor of lipase activity of Staphylococcus aureus.

    abstract::Staphylococcus aureus lipase (SAL) is known to possess broad substrate specificity for triacylglycerides. We found that a sub-minimum inhibitory concentration of farnesol (1000 mg L(-1)) inhibits this lipase activity on a Mueller-Hinton agar containing 1% Tween substrates. A quantitative lipase assay using p-nitrophen...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00772.x

    authors: Kuroda M,Nagasaki S,Ito R,Ohta T

    更新日期:2007-08-01 00:00:00

  • EnvC, a new lipoprotein of the cytoplasmic membrane of Escherichia coli.

    abstract::A gene product with an apparent molecular mass of approximately 39,000 Da can be identified in the cytoplasmic membrane of Escherichia coli upon expression of cloned envC. In this communication we report that the product was labelled with [3H]glycerol and [3H]palmitic acid, and a precursor molecule of increased molecu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06026.x

    authors: Seiffer D,Klein JR,Plapp R

    更新日期:1993-03-01 00:00:00

  • Nucleotide sequence of the ethidium efflux gene from Escherichia coli.

    abstract::The nucleotide sequence of the gene specifying the ethidium efflux system of Escherichia coli has been determined. The translated open reading frame has identified a membrane-bound polypeptide of 110 amino acids (11,960 Da) which shares 42% identity with a staphylococcal protein specifying resistance to ethidium. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(91)90338-b

    authors: Purewal AS

    更新日期:1991-08-01 00:00:00

  • Mobilizable narrow host range plasmids as natural suicide vectors enabling horizontal gene transfer among distantly related bacterial species.

    abstract::Klebsiella pneumoniae 287-w carries three small narrow host range (NHR) plasmids (pIGMS31, pIGMS32, and pIGRK), which could be maintained in several closely related species of Gammaproteobacteria, but not in Alphaproteobacteria. The plasmids contain different mobilization systems (MOB), whose activity in Escherichia c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02432.x

    authors: Smorawinska M,Szuplewska M,Zaleski P,Wawrzyniak P,Maj A,Plucienniczak A,Bartosik D

    更新日期:2012-01-01 00:00:00

  • Amino acid and nucleotide sequence, adjacent genes, and heterologous expression of hiracin JM79, a sec-dependent bacteriocin produced by Enterococcus hirae DCH5, isolated from Mallard ducks (Anas platyrhynchos).

    abstract::The primary structure of a bacteriocin produced by Enterococcus hirae DCH5 was determined by combined amino acid and DNA sequencing. Nucleotide analysis of a 2838-bp DNA fragment of E. hirae DCH5 revealed five putative ORFs. The first orf (hirJM79) encodes a 74-amino-acid peptide containing an N-terminal signal peptid...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00673.x

    authors: Sánchez J,Diep DB,Herranz C,Nes IF,Cintas LM,Hernández PE

    更新日期:2007-05-01 00:00:00

  • spyder, a new method for in silico design and assessment of 16S rRNA gene primers for molecular microbial ecology.

    abstract::Molecular microbial ecology studies are heavily reliant on 'Universal' 16S rRNA gene primers for elucidating microbial community structure and composition, and yet primer design and optimization is often overlooked. Primers that exhibit minor biases due to primer-template mismatches can substantially alter the pool of...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02302.x

    authors: Thomas MC,Thomas DK,Selinger LB,Inglis GD

    更新日期:2011-07-01 00:00:00

  • Modified peptidoglycan precursors produced by glycopeptide-resistant enterococci.

    abstract::Cytoplasmic precursors of the peptidoglycan biosynthetic pathway were purified from vancomycin-treated, glycopeptide-sensitive and -resistant strains of Enterococcus faecium. Resistance was due to production of a modified precursor, UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-lactate, where lactate was identified on the basi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90608-q

    authors: Messer J,Reynolds PE

    更新日期:1992-07-01 00:00:00

  • Influence of isolate origin and presence of various genes on biofilm formation by Enterococcus faecium.

    abstract::Enterococcus faecium, a major cause of nosocomial infections, is often isolated from conditions where biofilm is considered to be important in the establishment of infections. We investigated biofilm formation among E. faecium isolates from diverse sources and found that the occurrence and amount of biofilm formation ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12418

    authors: Almohamad S,Somarajan SR,Singh KV,Nallapareddy SR,Murray BE

    更新日期:2014-04-01 00:00:00

  • Expression of the phytochrome operon in Pseudomonas aeruginosa is dependent on the alternative sigma factor RpoS.

    abstract::Phytochromes are red/far-red light photoreceptors found in plants, cyanobacteria and heterotrophic bacteria. Biochemical analyses have established that the genes bphO and bphP (PA4116 and PA4117) of Pseudomonas aeruginosa encode both phytochrome components: BphO, a heme oxygenase that produces the linear tetrapyrrole ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.01058.x

    authors: Barkovits K,Harms A,Benkartek C,Smart JL,Frankenberg-Dinkel N

    更新日期:2008-03-01 00:00:00

  • Flow cytometric analysis of prolonged stress-dependent heterogeneity in bacterial cells.

    abstract::When bacterial cells are subjected to stress, cellular and population characteristics can change significantly. Rapid acquisition of these properties of cells in their native physiological state is necessary in order to precisely interpret their survival modes. The multiparametric analytical capability of flow cytomet...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01410.x

    authors: Sachidanandham R,Gin KY

    更新日期:2009-01-01 00:00:00

  • Degradation of chlorobiphenyls catalyzed by the bph-encoded biphenyl-2,3-dioxygenase and biphenyl-2,3-dihydrodiol-2,3-dehydrogenase of Pseudomonas sp. LB400.

    abstract::In order to characterize the metabolites produced in vivo by biphenyl-2,3-dioxygenase and biphenyl-2,3-dihydrodiol-2,3-dehydrogenase, the first two enzymes of the (polychloro)biphenyl catabolic pathway encoded by the bph locus of Pseudomonas sp. LB400, recombinant E. coli strains expressing the respective genes were c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07894.x

    authors: Seeger M,Timmis KN,Hofer B

    更新日期:1995-11-15 00:00:00

  • Serotype F double- and triple-converting phage insertionally inactivate the Staphylococcus aureus beta-toxin determinant by a common molecular mechanism.

    abstract::The precise molecular mechanism of Staphylococcus aureus beta-toxin inactivation by the serotype F triple-converting phage phi 42, phi A1 and phi A3 was investigated. Sequence analysis of the phi 42 (attP) and Staphylococcus aureus (attB) attachment sites and the left (attL) and right (attR) chromosomal/bacteriophage ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb05951.x

    authors: Carroll JD,Cafferkey MT,Coleman DC

    更新日期:1993-01-15 00:00:00