Influence of isolate origin and presence of various genes on biofilm formation by Enterococcus faecium.

abstract：:The biosynthesis of cephalosporins involving a thiozolidine ring expansion is catalyzed by deacetoxycephalosporin C synthase (DAOCS). In this study, three DAOCS isozymes were cloned and expressed as active enzymes together with Streptomyces jumonjinensis DAOCS that was newly isolated and partially characterized. The e...

journal_title：FEMS microbiology letters

authors： Chin HS,Sim J,Seah KI,Sim TS

更新日期：2003-01-28 00:00:00

abstract：:Tolaasin is a peptide toxin produced by Pseudomonas tolaasii and causes brown blotch disease of the cultivated mushrooms. Two types of ion channels were identified by the incorporation of tolaasin into lipid bilayer. The slope conductance of type 1 channel measured in the buffer containing 100 mM KCl was 150 pS with a...

journal_title：FEMS microbiology letters

authors： Cho KH,Kim YK

更新日期：2003-04-25 00:00:00

abstract：:The small noncoding RNA RyhB is a regulator of iron homeostasis in Escherichia coli. During iron limitation, it downregulates the expression of a number of iron-containing proteins, including enzymes of the tricarboxylic acid cycle and the respiratory chain. Because this infers a potential for RyhB to limit energy met...

journal_title：FEMS microbiology letters

authors： Bollinger CJ,Kallio PT

更新日期：2007-10-01 00:00:00

abstract：:Ammonium transporters form a conserved family of transport proteins and are widely distributed among all domains of life. The genome of Nitrosomonas europaea codes for a single gene (rh1) that belongs to the family of the AMT/Rh ammonium transporters. For the first time, this study provides functional and physiologica...

journal_title：FEMS microbiology letters

authors： Weidinger K,Neuhäuser B,Gilch S,Ludewig U,Meyer O,Schmidt I

更新日期：2007-08-01 00:00:00

abstract：:Salmonella enterica serovar Typhimurium is a food-borne pathogen and a leading cause of gastroenteritis in humans. Recently, we sequenced a phage-type DT108 strain (L945) and found reads with high similarity to both Salmonella typhi strain CT18 plasmid pHCM2 and bacteriophage SSU5. In this study, we completely sequenc...

journal_title：FEMS microbiology letters

authors： Octavia S,Sara J,Lan R

更新日期：2015-04-01 00:00:00

abstract：:Subtilin is an antimicrobial peptide of the lantibiotic family that is produced by Gram-positive Bacillus subtilis, and its biosynthesis involves expression of presubtilin which consists of a leader segment and a mature segment. The leader segment is unlike a typical sec-type general secretion signal, and its ability ...

journal_title：FEMS microbiology letters

authors： Paul LK,Izaguirre G,Hansen JN

更新日期：1999-07-01 00:00:00

abstract：:A number of enteric pathogens, including enteropathogenic (EPEC) and enterohemorrhagic (EHEC) Escherichia coli, Hafnia alvei, a strain of Citrobacter freundii, and rabbit EPEC strain RDEC-1 cause attaching-effacing (AE) lesions in the gut mucosa. These bacteria have a pathogenicity cassette (locus of enterocyte efface...

journal_title：FEMS microbiology letters

authors： Agin TS,Cantey JR,Boedeker EC,Wolf MK

更新日期：1996-11-01 00:00:00

abstract：:Recent studies have generated interest in the use of the homologous recombination system of bacteriophage lambda for genetic engineering. The system, called Red, consists primarily of three proteins: lambda exonuclease, which processively digests the 5'-ended strand of a dsDNA end; beta protein, which binds to ssDNA a...

journal_title：FEMS microbiology letters

authors： Poteete AR

更新日期：2001-07-10 00:00:00

abstract：:The gacA gene of the biocontrol strain Pseudomonas fluorescens CHA0 codes for a response regulator which, together with the sensor kinase GacS (=LemA), is required for the production of exoenzymes and secondary metabolites involved in biocontrol, including hydrogen cyanide (HCN). A gacA multicopy suppressor was isolat...

journal_title：FEMS microbiology letters

authors： Blumer C,Haas D

更新日期：2000-06-01 00:00:00

abstract：:Transposon mini-Tn7 vectors insert into the chromosome of several Gram-negative bacteria in a site-specific manner. Here, we showed the application of mini-Tn7 as single copy site-specific integration vector system for Xanthomonas campestris pv. campestris. The transposition of the mini-Tn7 into the bacterial genome w...

journal_title：FEMS microbiology letters

authors： Jittawuttipoka T,Buranajitpakorn S,Fuangthong M,Schweizer HP,Vattanaviboon P,Mongkolsuk S

更新日期：2009-09-01 00:00:00

abstract：:Trehalase was studied in Schizosaccharomyces pombe cells growing vegetatively on minimal medium and in sporulating cultures. Acid trehalase activity, measured at pH 4.2, was absent in vegetative cells and occurred only in asci, indicating that this activity represented the sporulation-specific trehalase reported previ...

journal_title：FEMS microbiology letters

authors： De Virgilio C,Müller J,Boller T,Wiemken A

更新日期：1991-11-01 00:00:00

abstract：:Alcaligenes sp. strain PPH degrades phenanthrene via 1-hydroxy-2-naphthoic acid (1-H2NA), 1,2-dihydroxynaphthalene (1,2-DHN), salicylic acid and catechol. Enzyme activity versus growth profile and heat stability studies suggested the presence of two distinct hydroxylases, namely 1-hydroxy-2-naphthoic acid hydroxylase ...

journal_title：FEMS microbiology letters

authors： Deveryshetty J,Phale PS

更新日期：2010-10-01 00:00:00

abstract：:The enzymatic activity of salivary amylase bound to the surface of several species of oral streptococci was determined by the production of acid from starch and by the degradation of maltotetraose to glucose in a coupled, spectrophotometric assay. Most strains able to bind amylase exhibited functional enzyme on their ...

journal_title：FEMS microbiology letters

authors： Douglas CW,Heath J,Gwynn JP

更新日期：1992-04-15 00:00:00

abstract：:The nucleotide sequence of the gene specifying the ethidium efflux system of Escherichia coli has been determined. The translated open reading frame has identified a membrane-bound polypeptide of 110 amino acids (11,960 Da) which shares 42% identity with a staphylococcal protein specifying resistance to ethidium. ...

journal_title：FEMS microbiology letters

authors： Purewal AS

更新日期：1991-08-01 00:00:00

abstract：:We used error-prone PCR to generate mutations in a subtilisin protease-encoding gene, and screened for recombinants that expressed temperature-sensitive (TS) variants. From the dozens of mutations that we detected in the recombinant genes we found that those mutations that affected aspartate-75 had the most profound e...

journal_title：FEMS microbiology letters

authors： Thompson VC,McGuire BE,Frier MS,Legg MSG,Dyer TW,Gudavicius G,Potter S,Nano FE

更新日期：2020-10-16 00:00:00

abstract：:Enterobacter cloacae CHE, a clinical strain with overproduced cephalosporinase was found to be highly resistant to the new cephalosporins, cefepime and cefpirome (MICs> or =128 microg ml(-1)). The strain was isolated from a child previously treated with cefepime. The catalytic efficiency of the purified enzyme with th...

journal_title：FEMS microbiology letters

authors： Barnaud G,Labia R,Raskine L,Sanson-Le Pors MJ,Philippon A,Arlet G

更新日期：2001-02-20 00:00:00

abstract：:Tetracycline resistance in Mycoplasma hominis and Ureaplasma urealyticum has been associated with the tetM determinant and has recently been increasing in incidence. We report here a rapid method for detection of the tetM determinant based on the use of the polymerase chain reaction (PCR) to amplify a 397-bp DNA fragm...

journal_title：FEMS microbiology letters

authors： Blanchard A,Crabb DM,Dybvig K,Duffy LB,Cassell GH

更新日期：1992-08-15 00:00:00

abstract：:To tackle antimicrobial resistance (AMR) is of outmost importance for the general population to understand the severity and the relevance of different routes of transmission. Respondents of different age groups, educational and occupational backgrounds, area of living, diet and household composition participated in an...

journal_title：FEMS microbiology letters

authors： Alexa Oniciuc EA,Likotrafiti E,Garre A,Ruiz L,Prieto M,Alvarez-Ordóñez A

更新日期：2019-09-01 00:00:00

abstract：:Mycoplasma fermentans has been reported to be pathogenic for man. All fourteen strains tested contain an insertion sequence-like element (ISLE) which may be present in multiple copies. To determine whether ISLE copies are similarly distributed in different strains of M. fermentans, restriction enzyme digest fragments ...

journal_title：FEMS microbiology letters

authors： Pitcher D,Hilbocus J

更新日期：1998-03-01 00:00:00

abstract：:Phylogenetically diverse rhizobial strains endemic to Tunisia were isolated from symbiotic nodules of Lotus creticus, growing in different arid extremophile geographical regions of Tunisia, and speciated using multiloci-phylogenetic analysis as Neorhizobium huautlense (LCK33, LCK35, LCO42 and LCO49), Ensifer numidicus...

journal_title：FEMS microbiology letters

authors： Rejili M,BenAbderrahim MA,Mars M,Sherrier JD

更新日期：2020-06-01 00:00:00

abstract：:Six clinical isolates of Enterobacter cloacae isolated in a Portuguese hospital, between April 1999 and November 2000, demonstrated resistance to almost all broad-spectrum cephalosporins, except to cefepime. These isolates were susceptible to quinolones and to aminoglycosides. Isoelectric focusing demonstrated product...

journal_title：FEMS microbiology letters

authors： Conceição T,Faria N,Lito L,Melo Cristino J,Salgado MJ,Duarte A

更新日期：2004-01-30 00:00:00

abstract：:Although Lentinula edodes is the second most important cultivated mushroom worldwide, most industrially cultivated strains have been identified only through traditional phenotypic analysis. Here, we report for the first time the use of sequence characterized amplified region (SCAR) markers for strain differentiation. ...

journal_title：FEMS microbiology letters

authors： Qin LH,Tan Q,Chen MJ,Pan YJ

更新日期：2006-04-01 00:00:00

abstract：:The alternative sigma factor sigmaH has been shown to play an important role in stress response and morphological differentiation in Streptomyces coelicolor. Its gene, sigH, is located in an operon with the gene encoding proposed anti-sigma factor UshX, and one of the promoters directing expression of the operon is de...

journal_title：FEMS microbiology letters

authors： Sevcikova B,Kormanec J

更新日期：2002-04-09 00:00:00

abstract：:A typing method for Clostridium difficile based on restriction fragment length polymorphisms (RFLP) is described. The technique utilizes commercially available Escherichia coli ribosomal ribonucleic acid (rRNA) as probe material. Probe labelling, hybridization and detection was performed using the Enhanced Chemilumine...

journal_title：FEMS microbiology letters

authors： Bowman RA,O'Neill GL,Riley TV

更新日期：1991-04-15 00:00:00

abstract：:Many bacteria are capable of accumulating intracellular granules of polyhydroxyalkanoates (PHA). In this work, we developed confocal microscopy analysis of bacterial cells to study changes in the diameters of cells as well as PHA granules during growth and PHA accumulation in the bacterium Cupriavidus necator H16 (for...

journal_title：FEMS microbiology letters

authors： Mravec F,Obruca S,Krzyzanek V,Sedlacek P,Hrubanova K,Samek O,Kucera D,Benesova P,Nebesarova J

更新日期：2016-05-01 00:00:00

abstract：:In Bacillus subtilis, utilisation of xylose, arabinose and ribose is controlled by the transcriptional factors XylR, AraR and RbsR, respectively. Here we apply the comparative approach to the analysis of these regulons in the Bacillus/Clostridium group. Evolutionary variability of operon structures is demonstrated and...

journal_title：FEMS microbiology letters

authors： Rodionov DA,Mironov AA,Gelfand MS

更新日期：2001-12-18 00:00:00

abstract：:Xylella fastidiosa is the causal agent of economically important plant diseases, including citrus variegated chlorosis and Pierce's disease. Hitherto, there has been no information on the molecular mechanisms controlling X. fastidiosa-plant interactions. To determine whether predicted open reading frames (ORFs) encodi...

journal_title：FEMS microbiology letters

authors： Scarpari LM,Lambais MR,Silva DS,Carraro DM,Carrer H

更新日期：2003-05-16 00:00:00

abstract：:Salmonella is a leading cause of waterborne diseases. Salmonella can survive for a long time in aquatic environments, and its persistence in the environment is of great concern to public health. Nonetheless, the presence and diversity of Salmonella in the aquatic environments in most areas remain relatively unknown. I...

journal_title：FEMS microbiology letters

authors： Huang KH,Hsu BM,Chou MY,Tsai HL,Kao PM,Wang HJ,Hsiao HY,Su MJ,Huang YL

更新日期：2014-03-01 00:00:00

abstract：:We report the construction of two broad host range promoter-probe plasmid vectors for rapid analysis of promoters in Gram-negative bacteria. The new vectors, pME4507 and pME4510, carry carbenicillin and gentamycin resistance genes, respectively, and are small sized (4 kb) with a flexible multiple cloning site to facil...

journal_title：FEMS microbiology letters

authors： Rist M,Kertesz MA

更新日期：1998-12-01 00:00:00

abstract：:It is often claimed that Antoni van Leeuwenhoek was secretive about his methods. However, closer examination of his letters suggests that this reputation was not always deserved. Some letters were not published in the Royal Society Proceedings, and others were edited. This paper describes the repetition of his experim...

journal_title：FEMS microbiology letters

authors： Robertson LA

更新日期：2019-08-01 00:00:00