Characterization of a large novel phage-like plasmid in Salmonella enterica serovar Typhimurium.

abstract：:To analyze regulation of the vanadium-dependent nitrogenase of Azotobacter vinelandii, plasmids carrying vnfE-, vnfH-, or vnfD-lacZ fusions were transferred to Escherichia coli. These genes were expressed only if VnfA was present. Deletions of the vnfE upstream region were constructed and comparison of a region necess...

journal_title：FEMS microbiology letters

authors： Woodley P,Buck M,Kennedy C

更新日期：1996-01-15 00:00:00

abstract：:Bacteriophages infecting Salmonella spp. were isolated from sewage using soft agar overlays containing three Salmonella serovars and assessed with regard to their potential to control food-borne salmonellae. Two distinct phages, as defined by plaque morphology, structure and host range, were obtained from a single sam...

journal_title：FEMS microbiology letters

authors： Carey-Smith GV,Billington C,Cornelius AJ,Hudson JA,Heinemann JA

更新日期：2006-05-01 00:00:00

abstract：:An investigation of mortality of piglets through clinical signs, post-mortem, histopathology and bacteriological analyses revealed the causal organism to be Escherichia coli, mainly O149:K91:K88 which belongs to the enterotoxigenic biotypes. Molecular characterization and epidemiologic analysis elucidated it as shiga-...

journal_title：FEMS microbiology letters

authors： Kanengoni AT,Thomas R,Gelaw AK,Madoroba E

更新日期：2017-02-01 00:00:00

abstract：:The phosphatidylcholine (PC) content of Aspergillus nidulans choC was varied by growing the auxotroph in medium containing various concentrations of choline chloride. Direct linear correlations were observed between PC content and in vivo chitin synthase activity, between in vivo chitin synthase activity and mean hyph...

journal_title：FEMS microbiology letters

authors： Binks PR,Robson GD,Goosey MW,Trinci AP

更新日期：1991-10-01 00:00:00

abstract：:The genes encoding transferrin-binding proteins (TBPs) 1 and 2 of Neisseria meningitidis and N. gonorrhoeae were used as model loci in a novel method of cloning (twin N-terminal polymerase chain reaction; TNT-PCR) involving amplification between the 5' ends of two genes. Primers were based on N-terminal amino-acid seq...

journal_title：FEMS microbiology letters

authors： Wilton J,Ala'Aldeen D,Palmer HM,Borriello SP

更新日期：1993-02-15 00:00:00

abstract：:Three strains of Streptococcus uberis isolated from dairy cows with mastitis were co-cultured with a bovine mammary epithelial cell line (MAC-T) in Dulbecco's modified Eagle's medium without fetal bovine serum. Protein profiles from culture supernatants and bacterial pellets among different treatments were compared by...

journal_title：FEMS microbiology letters

authors： Fang W,Luther DA,Oliver SP

更新日期：1998-09-15 00:00:00

abstract：:The branched-chain amino acid permease Bap2p is a transport system for leucine, isoleucine, and valine in Saccharomyces cerevisiae, and its synthesis is regulated transcriptionally. However, the downregulation mechanisms of Bap2p have not been established. Here we demonstrate that the C-terminal region of Bap2p plays ...

journal_title：FEMS microbiology letters

authors： Omura F,Kodama Y,Ashikari T

更新日期：2001-01-15 00:00:00

abstract：:Binding of meningitis-causing Escherichia coli K1 to human brain microvascular endothelial cells (HBMEC) contributes to traversal of the blood-brain barrier, which occurs in part by the mannose-sensitive binding of FimH. In this study, we showed that FimH also binds to HBMEC, independent of mannose, and identified ATP...

journal_title：FEMS microbiology letters

authors： Shin S,Kim KS

更新日期：2010-02-01 00:00:00

abstract：:A novel Toxoplasma gondii interspersed repeat element (TgIRE), present in most of the tachyzoite chromosomes, was characterised. Two regions on the TgIRE sequence showed high identity to two different T. gondii expressed sequence tag cDNAs of unknown function, which seems to be TgIRE pseudogenes. Two set of primers we...

journal_title：FEMS microbiology letters

authors： Echeverria PC,Rojas PA,Martin V,Guarnera EA,Pszenny V,Angel SO

更新日期：2000-03-01 00:00:00

abstract：:A cordycepin-resistant mutant strain of Saccharomyces cerevisiae (CD-R2) was found to be deficient in adenosine kinase. This mutant accumulated S-adenosylhomocysteine during growth in the presence of exogenous adenosine and it grew in a pseudohyphal manner in the presence of this nucleotide. ...

journal_title：FEMS microbiology letters

authors： Iwashima A,Ogata M,Nosaka K,Nishimura H,Hasegawa T

更新日期：1995-03-15 00:00:00

abstract：:Atypical enteropathogenic Escherichia coli (aEPEC) is comprised of a large heterogeneous group of strains and serotypes that carry the intimin gene (eae) but no other EPEC virulence factors. In a previous study, we examined a few aEPEC strains of O157:H16 serotype from the U.S. and France and found these to be nearly ...

journal_title：FEMS microbiology letters

authors： Feng PC,Keys C,Lacher DW,Beutin L,Bentancor A,Heuvelink A,Afset JE,Rumi V,Monday S

更新日期：2012-12-01 00:00:00

abstract：:The effectiveness of an educational board game developed to teach the pharmacology of antimicrobial drugs to medical students was compared with the lecture-based seminar as a supplemental tool to improve short- and long-term knowledge retention and the perception of the learning method by students. A group of 124 stud...

journal_title：FEMS microbiology letters

authors： Karbownik MS,Wiktorowska-Owczarek A,Kowalczyk E,Kwarta P,Mokros Ł,Pietras T

更新日期：2016-04-01 00:00:00

abstract：:The assessment of scientific research is essentially based on several metric parameters, the so-termed Impact Factor perhaps being the predominant one. Despite well-founded criticisms and the wide opposition of reputed scientists, this procedure has become a tool of scientific policy, and is applied in editorial proce...

journal_title：FEMS microbiology letters

authors： Argüelles JC,Argüelles-Prieto R

更新日期：2019-06-01 00:00:00

abstract：:The biosynthesis of cephalosporins involving a thiozolidine ring expansion is catalyzed by deacetoxycephalosporin C synthase (DAOCS). In this study, three DAOCS isozymes were cloned and expressed as active enzymes together with Streptomyces jumonjinensis DAOCS that was newly isolated and partially characterized. The e...

journal_title：FEMS microbiology letters

authors： Chin HS,Sim J,Seah KI,Sim TS

更新日期：2003-01-28 00:00:00

abstract：:The influence of nutritional and physical stress on sporulation, conidial germination and vegetative biomass of Ophiocordyceps sinensis, one of the most important medicinal fungi in China and now globally, was evaluated using a two-stage culture method. All the treatments, except nutrient deprivation, enhanced conidia...

journal_title：FEMS microbiology letters

authors： Ren SY,Yao YJ

更新日期：2013-09-01 00:00:00

abstract：:We isolated and characterized nodA genes from photosynthetic and non-photosynthetic rhizobia nodulating the legume genus Aeschynomene, and found that the nodA sequence from photosynthetic stem-nodulating bacteria was phylogenetically distant from the other already described nodA genes. Characterization of the photosyn...

journal_title：FEMS microbiology letters

authors： Chaintreuil C,Boivin C,Dreyfus B,Giraud E

更新日期：2001-01-01 00:00:00

abstract：:In Corynebacterium glutamicum the promoter of the araBAD Escherichia coli gene is positively regulated by both arabinose and the araC gene product, as it is the case in the natural host. If the L-arabinose inducer and an active araC gene are present, significant amounts of araBAD promoter expression take place in the ...

journal_title：FEMS microbiology letters

authors： Ben-Samoun K,Leblon G,Reyes O

更新日期：1999-05-01 00:00:00

abstract：:Efficient type-III secretion depends on cytosolic molecular chaperones, which bind specifically to the translocators and effectors. In the past there has been a tendency to shoe-horn all type-III-secretion chaperones into a single structural and functional class. However, we have shown that the LcrH/SycD-like chaperon...

journal_title：FEMS microbiology letters

authors： Pallen MJ,Francis MS,Fütterer K

更新日期：2003-06-06 00:00:00

abstract：:Surrogate methods for detecting lateral gene transfer are those that do not require inference of phylogenetic trees. Herein I apply four such methods to identify open reading frames (ORFs) in the genome of Escherichia coli K12 that may have arisen by lateral gene transfer. Only two of these methods detect the same ORF...

journal_title：FEMS microbiology letters

authors： Ragan MA

更新日期：2001-07-24 00:00:00

abstract：:The genes mphA and mphB encode macrolide 2'-phosphotransferases I and II, respectively, and they confer resistance to macrolide antibiotics in Escherichia coli. To study the expression of these genes in Gram-positive bacteria, we constructed recombinant plasmids that consisted of an mph gene and the pUB110 vector in B...

journal_title：FEMS microbiology letters

authors： Noguchi N,Tamura Y,Katayama J,Narui K

更新日期：1998-02-15 00:00:00

abstract：:A composite transposon is a mobile genetic element consisting of two insertion sequences (ISs) flanking a segment of cargo DNA often containing antibiotic resistance (AR) genes. Composite transposons can move as a discreet unit. There have been recently several reports on a novel mechanism of movement of an IS26-based...

journal_title：FEMS microbiology letters

authors： Tansirichaiya S,Mullany P,Roberts AP

更新日期：2016-09-01 00:00:00

abstract：:Bacterial pathogens face constant challenges from DNA-damaging agents generated by host phagocytes. Although Borrelia burgdorferi appears to have much fewer DNA repair enzymes than pathogens with larger genomes, it does contain homologues of uvrA and uvrB (subunits A and B of excinuclease ABC). As a first step to expl...

journal_title：FEMS microbiology letters

authors： Sambir M,Ivanova LB,Bryksin AV,Godfrey HP,Cabello FC

更新日期：2011-04-01 00:00:00

abstract：:Food-borne diseases are a threat to human health and can cause severe economic losses. Nowadays, in a growing and increasingly interconnected world, food-borne diseases need to be dealt with in a global manner. In order to tackle this issue, it is essential to consider all possible entry routes of human pathogens into...

journal_title：FEMS microbiology letters

authors： Schierstaedt J,Grosch R,Schikora A

更新日期：2019-12-01 00:00:00

abstract：:Marine snow aggregates are microbial hotspots that support high bacterial abundance and activities. We conducted laboratory experiments to compare cell-specific bacterial protein production (BPP) and protease activity between free-living and attached bacteria. Natural bacterial assemblages attached to model aggregates...

journal_title：FEMS microbiology letters

authors： Grossart HP,Tang KW,Kiørboe T,Ploug H

更新日期：2007-01-01 00:00:00

abstract：:Multidrug efflux pumps contribute to multiple antibiotic resistance in Pseudomonas aeruginosa. Pump expression usually has been quantified by Western blotting. Quantitative real-time polymerase chain reaction has been developed to measure mRNA expression for genes of interest. Whether this method correlates with pump ...

journal_title：FEMS microbiology letters

authors： Yoneda K,Chikumi H,Murata T,Gotoh N,Yamamoto H,Fujiwara H,Nishino T,Shimizu E

更新日期：2005-02-01 00:00:00

abstract：:A previous study [DeVault et al. (1991) Mol. Microbiol. 5, 2503-2509] suggested that growth of Pseudomonas aeruginosa in glucose-containing medium represses algD gene transcription. In this study, growth of P. aeruginosa in rich medium containing glucose or gluconate increased alginate production and algD transcriptio...

journal_title：FEMS microbiology letters

authors： Ma JF,Phibbs PV,Hassett DJ

更新日期：1997-03-15 00:00:00

abstract：:Growth of Pseudomonas aeruginosa on acyclic terpene alcohols (citronellol) and on other methyl-branched compounds such as leucine or isovalerate requires a functional leucine/isovalerate utilization (Liu) pathway. In this study, we investigated the liuABCDE gene cluster by insertion mutant analysis, heterologous expre...

journal_title：FEMS microbiology letters

authors： Förster-Fromme K,Jendrossek D

更新日期：2008-09-01 00:00:00

abstract：:In order to characterize the metabolites produced in vivo by biphenyl-2,3-dioxygenase and biphenyl-2,3-dihydrodiol-2,3-dehydrogenase, the first two enzymes of the (polychloro)biphenyl catabolic pathway encoded by the bph locus of Pseudomonas sp. LB400, recombinant E. coli strains expressing the respective genes were c...

journal_title：FEMS microbiology letters

authors： Seeger M,Timmis KN,Hofer B

更新日期：1995-11-15 00:00:00

abstract：:Biochemical responses to cold and osmotic stresses overlap because each decreases the availability of free water. Since RNA-binding proteins are known to accumulate following cold stress and play key roles in regulating transcription termination, the effect of osmotic stress on expression of RNA-binding proteins was e...

journal_title：FEMS microbiology letters

authors： Mori S,Castoreno A,Mulligan ME,Lammers PJ

更新日期：2003-10-24 00:00:00

abstract：:High resistance to class IIa bacteriocins in Listeria monocytogenes has been clearly linked to lack of expression of the mptACD operon, encoding the EIIt Man mannose PTS permease. Also, intermediate resistance has been associated with membrane phospholipid modifications in the spontaneous mutants L. monocytogenes B73-...

journal_title：FEMS microbiology letters

authors： Arous S,Dalet K,Héchard Y

更新日期：2004-09-01 00:00:00