Iterative selection from a Salmonella typhimurium cosmid library can lead to the isolation of an atypically small plasmid.

abstract：:A gene product with an apparent molecular mass of approximately 39,000 Da can be identified in the cytoplasmic membrane of Escherichia coli upon expression of cloned envC. In this communication we report that the product was labelled with [3H]glycerol and [3H]palmitic acid, and a precursor molecule of increased molecu...

journal_title：FEMS microbiology letters

authors： Seiffer D,Klein JR,Plapp R

更新日期：1993-03-01 00:00:00

abstract：:The miniplasmid profiles of 18 Bacillus thuringiensis strains belonging to 8 different serotypes were determined using an alkaline hydrolysis method for isolation of low molecular mass plasmids. Nearly all the strains contained covalently closed circular (CCC) DNA species ranging from 2 to 5 species per strain and fro...

journal_title：FEMS microbiology letters

authors： Marin R,Tanguay RM,Valéro J,Letarte R,Bellemare G

更新日期：1992-07-15 00:00:00

abstract：:Reduction of D-fructose to D-mannitol by whole-cell biotransformation with recombinant resting cells of Corynebacterium glutamicum ATCC13032 requires the coexpression of mdh and fdh, which encode mannitol and formate dehydrogenases, respectively. However, d-mannitol formation is limited by the uptake of d-fructose in ...

journal_title：FEMS microbiology letters

authors： Bäumchen C,Krings E,Bringer S,Eggeling L,Sahm H

更新日期：2009-01-01 00:00:00

abstract：:Phylogenetic relationships between Tuber pseudoexcavatum and other Tuber species were investigated by studying the sequences of four genes: 5.8S-ITS2, beta-tubulin, protein kinase C and elongation factor 1alpha. The four phylogenetic trees allowed to differentiate the black truffle clade, composed of two subclades, on...

journal_title：FEMS microbiology letters

authors： Wang Y,Tan ZM,Zhang DC,Murat C,Jeandroz S,Le Tacon F

更新日期：2006-06-01 00:00:00

abstract：:The multiple antibiotic resistance operon (marRAB) is a member of the multidrug resistance (mdr) systems. Similar to other mdr systems, this operon when induced encodes resistance to structurally and functionally unrelated antibiotics. marRAB has been shown to be conserved in the family Enterobacteriaceae, but within ...

journal_title：FEMS microbiology letters

authors： Kunonga NI,Sobieski RJ,Crupper SS

更新日期：2000-06-15 00:00:00

abstract：:beta-Glucosidase in Aspergillus nidulans was found to be both intracellular and extracellular. The intracellular beta-glucosidase was synthesized after the exhaustion of carbon source in the medium. The extracellular enzyme appeared with autolysis of the mycelium. Biosynthesis of beta-glucosidase was not induced by va...

journal_title：FEMS microbiology letters

authors： Lee J,Kwon KS,Hah YC

更新日期：1996-01-01 00:00:00

abstract：:Agrobacterium tumefaciens is the causal agent of crown gall disease and is a vector for DNA transfer in transgenic plants. The transformation process by A. tumefaciens has been widely studied, but the attachment stage has not been well characterized. Most measurements of attachment have used microscopy and colony coun...

journal_title：FEMS microbiology letters

authors： Petrovicheva A,Joyner J,Muth TR

更新日期：2017-10-02 00:00:00

abstract：:Members of the Streptococcus bovis group are frequent colonizers of the intestinal tract, which can also cause endocarditis. However, their ability to adhere to and colonize host tissues and the factors associated with pathogenicity are largely unknown. Here, we assessed 17 endocarditis-derived human isolates [identif...

journal_title：FEMS microbiology letters

authors： Sillanpää J,Nallapareddy SR,Singh KV,Ferraro MJ,Murray BE

更新日期：2008-12-01 00:00:00

abstract：:Recent studies have generated interest in the use of the homologous recombination system of bacteriophage lambda for genetic engineering. The system, called Red, consists primarily of three proteins: lambda exonuclease, which processively digests the 5'-ended strand of a dsDNA end; beta protein, which binds to ssDNA a...

journal_title：FEMS microbiology letters

authors： Poteete AR

更新日期：2001-07-10 00:00:00

abstract：:In Taiwan, leptospirosis is caused mainly by Leptospira santarosai serovar shermani. Suppression subtractive hybridization was employed to isolate DNA fragments present in pathogenic L. santarosai serovar shermani but absent in non-pathogenic L. biflexa serovar patoc. Analysis of 23 subtracted DNA clones revealed 25 g...

journal_title：FEMS microbiology letters

authors： Hsieh WJ,Pan MJ

更新日期：2004-06-01 00:00:00

abstract：:We have observed significant cell lysis upon temperature up-shift of recombinant Escherichia coli cultures harboring CI857-repressed lambda-based expression vectors. This event, that becomes evident about 30-40 min after the heat shock, takes place when using the lambda promoter system in Ind- lysogenic strains, but n...

journal_title：FEMS microbiology letters

authors： Hoffmann F,Arís A,Carbonell X,Rohde M,Corchero JL,Rinas U,Villaverde A

更新日期：1999-08-15 00:00:00

abstract：:The discovery of nitrite-dependent anaerobic methane oxidation (n-damo) mediated by 'Candidatus Methylomirabilis oxyfera' with nitrite and methane as substrates has connected biogeochemical carbon and nitrogen cycles in a new way. The paddy fields often carry substantial methane and nitrate, thus may be a favorable ha...

journal_title：FEMS microbiology letters

authors： Zhou L,Wang Y,Long XE,Guo J,Zhu G

更新日期：2014-11-01 00:00:00

abstract：:In Salmonella typhimurium the metE and metR promoters overlap and are divergently transcribed. Three tandem repeats of an 8 bp sequence defined previously as the metE operator site for MetJ-mediated repression also overlap the -35 region of the metR promoter. Starting with a metE-lacZ.metR-galK double gene fusion, sit...

journal_title：FEMS microbiology letters

authors： Wu WF,Urbanowski ML,Stauffer GV

更新日期：1993-04-01 00:00:00

abstract：:Gut microbiota has been suggested as an important component of host defense. However, microbiota in other parts of the body have rarely been characterized. In our present work, we give a detailed view of the body of Spodoptera exigua larvae, the midgut with intestinal contents (MG), midgut excluding intestinal content...

journal_title：FEMS microbiology letters

authors： Gao X,Li W,Luo J,Zhang L,Ji J,Zhu X,Wang L,Zhang K,Zhang S,Cui J

更新日期：2020-02-01 00:00:00

abstract：:A novel transposon mutagenesis system for the phytopathogenic bacteria Xanthomonas oryzae pv. oryzae (Xoo) and X. campestris pv. campestris (Xcc) was developed using a Tn5-based transposome. A highly efficient transformation up to 10(6) transformants per microg transposon DNA was obtained. Southern blot and thermal as...

journal_title：FEMS microbiology letters

authors： Sun Q,Wu W,Qian W,Hu J,Fang R,He C

更新日期：2003-09-12 00:00:00

abstract：:A semi-automated method has been developed for the quantification and measurement of conidia discharged by the aphid pathogen Erynia neoaphidis. This was used to compare conidiation by E. neoaphidis-mycosed pea aphid cadavers, mycelial plugs cut from agar plates, mycelial pellets from shake flasks and by mycelial pell...

journal_title：FEMS microbiology letters

authors： Bonner TJ,Pell JK,Gray SN

更新日期：2003-03-14 00:00:00

abstract：:We have analyzed 81 isolates of Mycoplasma agalactiae from four different regions of Italy between 1990 and 1995 in order to identify antigenic differences through SDS-PAGE and Western blotting and chromosomal DNA restriction endonuclease cleavage pattern differences. Antigenic variability in M. agalactiae isolates wa...

journal_title：FEMS microbiology letters

authors： Tola S,Idini G,Manunta D,Casciano I,Rocchigiani AM,Angioi A,Leori G

更新日期：1996-10-01 00:00:00

abstract：:Adenylate kinase isoenzymes localised in the mitochondria and in the cytosol have been detected in extracts of glucose-grown Aspergillus nidulans using specific staining after electrophoresis on cellulose acetate. The isoenzymes have similar Km values for AMP, ADP and MgATP2- but may differ in the mechanism used for i...

journal_title：FEMS microbiology letters

authors： Hoang VQ,Jaklitsch W,Scrutton MC

更新日期：1991-03-01 00:00:00

abstract：:Naturally occurring antimicrobial cationic polypeptides play a major role in innate and adaptive immunity. These polypeptides are found to be either linear and unstructured or structured through disulfide bonds. Among the structured antimicrobial polypeptides, defensins comprise a family of cysteine-rich cationic poly...

journal_title：FEMS microbiology letters

authors： Raj PA,Dentino AR

更新日期：2002-01-02 00:00:00

abstract：:Yeast two-hybrid and Far Western analyses were used to detect interactions between Bacillus subtilis spores' nutrient germinant receptor proteins and proteins encoded by the spoVA operon, all of which are involved in spore germination and located in the spores' inner membrane. These analyses indicated that two subunit...

journal_title：FEMS microbiology letters

authors： Vepachedu VR,Setlow P

更新日期：2007-09-01 00:00:00

abstract：:A 1.8-kb cryptic plasmid pFR18 was isolated from Leuconostoc mesenteroides ssp. mesenteroides FR52 and characterized. The identification of single-stranded DNA intermediate (ssDNA) in Leuconostoc demonstrated that the replication of pFR18 is directed by a rolling-circle mechanism (RCR). Sequence analysis revealed a si...

journal_title：FEMS microbiology letters

authors： Biet F,Cenatiempo Y,Fremaux C

更新日期：1999-10-15 00:00:00

abstract：:Escherichia coli is the most common organism associated with asymptomatic bacteriuria (ABU) in humans. In contrast to uropathogenic E. coli (UPEC) that cause symptomatic urinary tract infection, very little is known about the mechanisms by which these strains colonize the urinary tract. Here, we have investigated the ...

journal_title：FEMS microbiology letters

authors： Hancock V,Ferrières L,Klemm P

更新日期：2007-02-01 00:00:00

abstract：:Using electron microscopy, pili with at least two distinct morphologies were observed on strains of Pasteurella multocida isolated from pigs with atrophic rhinitis. Rigid pili were found on 60-80% of all cells observed. These pili had a strong tendency to lie flat along the side of the outer cell membrane of P. multoc...

journal_title：FEMS microbiology letters

authors： Isaacson RE,Trigo E

更新日期：1995-10-15 00:00:00

abstract：:The polymerase chain reaction (PCR)-based procedures of randomly amplified polymorphic DNA (RAPD) and repetitive element (RE)-based PCR were used to amplify total DNA prepared from each of 62 clinical Serratia marcescens isolates. Three different random primers, designated 1060, 1254 and 1283, were used individually i...

journal_title：FEMS microbiology letters

authors： Patton TG,Katz S,Sobieski RJ,Crupper SS

更新日期：2001-01-01 00:00:00

abstract：:The genome size of Actinobacillus pleuropneumoniae was determined by pulsed field gel electrophoresis of AscI and ApaI digested chromosomal DNA. The genome size of the type strain 4074T (serotype 1) was determined to be 2404 +/- 40 kb. The chromosome sizes for the reference strains of the other serotypes range between...

journal_title：FEMS microbiology letters

authors： Chevallier B,Dugourd D,Tarasiuk K,Harel J,Gottschalk M,Kobisch M,Frey J

更新日期：1998-03-15 00:00:00

abstract：:Previously-developed PCR protocols specific for the 16S rRNA gene of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli were adapted for the detection of these species in human faeces, following DNA extraction and purification using mini-prep columns. The limits of detection in seeded faeces f...

journal_title：FEMS microbiology letters

authors： Mikosza AS,La T,Margawani KR,Brooke CJ,Hampson DJ

更新日期：2001-04-13 00:00:00

abstract：:An isolation procedure was developed to provide within one day microcystin-LR, a cyclic heptapeptide toxin from Microcystis aeruginosa PCC 7806. After ODS (octadecylsilyl) solid phase extraction, the crude toxin fraction was chromatographed using a strong anion exchange column. The toxin was eluted with 0.02 M ammoniu...

journal_title：FEMS microbiology letters

authors： Martin C,Sivonen K,Matern U,Dierstein R,Weckesser J

更新日期：1990-03-01 00:00:00

abstract：:Escherichia coli heat-labile enterotoxin B subunit (EtxB) has been proposed as a potential protein carrier for the delivery of heterologous peptides to target cells, particularly for the oral delivery of epitopes to the mucosal immune system. In this study, two extensions to the C-terminus of EtxB were genetically eng...

journal_title：FEMS microbiology letters

authors： Marcello A,Loregian A,Palù G,Hirst TR

更新日期：1994-03-15 00:00:00

abstract：:Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) represents a simple reliable approach for rapid bacterial identification based on specific peptide/protein fingerprints. However, cell-wall characteristics of mycobacterial species, and their well known stability, complicate MA...

journal_title：FEMS microbiology letters

authors： Balážová T,Makovcová J,Šedo O,Slaný M,Faldyna M,Zdráhal Z

更新日期：2014-04-01 00:00:00

abstract：:An inducible mycelial beta-glucosidase from Scytalidum thermophilum was characterized. The enzyme exhibited a pI of 6.5, a carbohydrate content of 15%, and an apparent molecular mass of about 40 kDa. Optima of temperature and pH were 60 degrees C and 6.5, respectively. The enzyme was stable up to 1 h at 50 degrees C a...

journal_title：FEMS microbiology letters

authors： Zanoelo FF,Polizeli Mde L,Terenzi HF,Jorge JA

更新日期：2004-11-15 00:00:00