A new subtype of the metalloprotease toxin gene and the incidence of the three bft subtypes among Bacteroides fragilis isolates in Japan.

abstract：:FlgM inhibits the flagella-specific sigma factor FliA and is involved in the mouse-virulence of Salmonella typhimurium. In recent experiments, we observed that: (i) a flgM gene that could function to negatively regulate flagella synthesis was present in a variety of salmonellae; and (ii) the flgM gene derived from Sal...

journal_title：FEMS microbiology letters

authors： Schmitt CK,Darnell SC,O'Brien AD

更新日期：1996-01-15 00:00:00

abstract：:It was shown that virulent Mycobacterium tuberculosis H37Rv induces necrosis of infected RAW264 cells at 24 h post infection while avirulent H37Ra and an attenuated H37Rv mutant that is deficient for RD1 region (H37RvDeltaRD1) cause less necrosis of the infected cells. While H37Rv caused damage of the mitochondrial in...

journal_title：FEMS microbiology letters

authors： Kaku T,Kawamura I,Uchiyama R,Kurenuma T,Mitsuyama M

更新日期：2007-09-01 00:00:00

abstract：:The Mycobacterium marinum G13 promoter is a sigma 70-like promoter that is more active by green fluorescent protein (gfp) differential fluorescence induction (DFI) assays when M. marium resides in an intracellular compartment as compared with growth in media. In assays using DFI, we found that the mycobacterial G13 pr...

journal_title：FEMS microbiology letters

authors： Gall K,Barker LP

更新日期：2006-02-01 00:00:00

abstract：:The primary structure of a bacteriocin produced by Enterococcus hirae DCH5 was determined by combined amino acid and DNA sequencing. Nucleotide analysis of a 2838-bp DNA fragment of E. hirae DCH5 revealed five putative ORFs. The first orf (hirJM79) encodes a 74-amino-acid peptide containing an N-terminal signal peptid...

journal_title：FEMS microbiology letters

authors： Sánchez J,Diep DB,Herranz C,Nes IF,Cintas LM,Hernández PE

更新日期：2007-05-01 00:00:00

abstract：:In Streptomyces cyanogenus S136 gene cluster for biosynthesis of polyglycosylated angucycline landomycin A (LaA), a divergently oriented gene pair for a TetR-family regulator (lanK) and an efflux protein (lanJ) is located, whose functions remained obscure. Overexpression and disruption studies showed that lanK and lan...

journal_title：FEMS microbiology letters

authors： Ostash I,Ostash B,Luzhetskyy A,Bechthold A,Walker S,Fedorenko V

更新日期：2008-08-01 00:00:00

abstract：:The extensively discussed idea of oxidative stress development under antibiotic treatment was confirmed using an antioxidant gene expression (soxRS-, oxyR-regulon) approach, including microaerobic cultivation conditions. The killing action of antibiotics and their ability to cause peroxide oxidative stress in Escheric...

journal_title：FEMS microbiology letters

authors： Akhova AV,Tkachenko AG

更新日期：2014-04-01 00:00:00

abstract：:Fourteen solvent-sensitive transposon mutants were generated from the solvent-tolerant Pseudomonas putida strain S12 by applying the TnMod-KmO mutagenesis system. These mutants were unable to grow in the presence of octanol and toluene. By cloning the region flanking the transposon insertion point a partial sequence o...

journal_title：FEMS microbiology letters

authors： Kieboom J,Bruinenberg R,Keizer-Gunnink I,de Bont JA

更新日期：2001-05-01 00:00:00

abstract：:Two plasmids, pEM4 (IncFI) determining virulence and pEM6 (IncI) determining colicine I production and resistance to tetracycline, have been found in a pathogenic strain of Citrobacter freundii. Even though pEM4 and pEM6 plasmids are conjugative and transfer themselves very efficiently during conjugation, a high cotra...

journal_title：FEMS microbiology letters

authors： Morzejko E,Panek E,Cebrat S

更新日期：1990-03-01 00:00:00

abstract：:The objective of this work was to analyze the effects of thermosonication (TS) on induction of a viable but non-culturable (VBNC) state in Salmonella Typhimurium and to examine incubation factors affecting subsequent resuscitation of cells. A TS treatment of 380 W at 53°C for 30 min induced the VBNC state in S. Typhim...

journal_title：FEMS microbiology letters

authors： Liao H,Jiang L,Zhang R

更新日期：2018-02-01 00:00:00

abstract：:A broad range of different chemical and pharmaceutical compounds have been produced in microbial cell factories. To compete with traditional crude oil based production processes, the use of complex alternative raw materials such as lignocellulosic biomass, waste streams and utilization of CO2 in gas fermentations has ...

journal_title：FEMS microbiology letters

authors： Novak K,Pflügl S

更新日期：2018-10-01 00:00:00

abstract：:High quality Fourier transform infrared (FTIR) spectra were acquired from living Micrasterias hardyi cells maintained in an IR transparent flow-through cell using a FTIR microscope coupled to a synchrotron light source. Spectral maps of living, nutrient-replete cells showed band intensities consistent with the known l...

journal_title：FEMS microbiology letters

authors： Heraud P,Wood BR,Tobin MJ,Beardall J,McNaughton D

更新日期：2005-08-15 00:00:00

abstract：:Most Moraxella catarrhalis isolates express a highly-conserved outer membrane protein of 453 residues designated OMPCD, which has been previously shown to mediate binding to A549 human lung cells. Here, it is reported that two distinct domains of the M. catarrhalis strain O35E OMPCD protein specify adherence. Truncate...

journal_title：FEMS microbiology letters

authors： Akimana C,Lafontaine ER

更新日期：2007-06-01 00:00:00

abstract：:In the transition to the post-petroleum economy, there is a growing demand for novel enzymes with high process performances to replace traditional chemistry with a more 'green' approach. To date, microorganisms encompass the richest source of industrial biocatalysts, but the Earth-living microbiota remains largely unt...

journal_title：FEMS microbiology letters

authors： Berini F,Casciello C,Marcone GL,Marinelli F

更新日期：2017-11-15 00:00:00

abstract：:Amylosucrase produces an insoluble alpha-1,4-linked glucan from sucrose, releasing fructose. In addition to polymerisation, in the presence of sucrose as sole substrate, amylosucrase catalyses sucrose hydrolysis and oligosaccharide synthesis in significant proportions. The effects of both glycogen acceptor and sucrose...

journal_title：FEMS microbiology letters

authors： Potocki de Montalk G,Remaud-Simeon M,Willemot RM,Monsan P

更新日期：2000-05-01 00:00:00

abstract：:To increase knowledge on haem biosynthesis in filamentous fungi like Aspergillus niger, pathway-specific gene expression in response to haem and haem intermediates was analysed. This analysis showed that iron, 5'-aminolevulinic acid (ALA) and possibly haem control haem biosynthesis mostly via modulating expression of ...

journal_title：FEMS microbiology letters

authors： Franken AC,Lokman BC,Ram AF,van den Hondel CA,de Weert S,Punt PJ

更新日期：2012-10-01 00:00:00

abstract：:Mycobacterium sp. PYR-1 was previously isolated in our laboratory and was shown to be able to mineralize high molecular mass polycyclic aromatic hydrocarbons (PAHs) [Heitkamp and Cerniglia, (1988) Appl. Environ. Microbiol. 54, 1612-1614]. In this research, the 16S rRNA gene (rDNA) of this strain was amplified by polym...

journal_title：FEMS microbiology letters

authors： Wang RF,Cao WW,Cerniglia CE

更新日期：1995-07-15 00:00:00

abstract：:This study was undertaken to investigate the binding activity of Streptococcus suis serotype 2 to albumin. Using flow cytometry we observed a binding activity of S. suis to albumin for virulent as well as for avirulent isolates. Western immunoblots analysis revealed that a 39-kDa S. suis protein was responsible, at le...

journal_title：FEMS microbiology letters

authors： Quessy S,Busque P,Higgins R,Jacques M,Dubreuil JD

更新日期：1997-02-15 00:00:00

abstract：:The role of the flagellum and chemotactic motility of Vibrio anguillarum for phagocytosis by and intracellular survival in fish macrophages was determined using a wild-type strain, a mutant without the flagellum, a mutant with a truncated flagellum and a non-chemotactic mutant. For all strains, the numbers of intracel...

journal_title：FEMS microbiology letters

authors： Larsen MH,Boesen HT

更新日期：2001-09-25 00:00:00

abstract：:Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) represents a simple reliable approach for rapid bacterial identification based on specific peptide/protein fingerprints. However, cell-wall characteristics of mycobacterial species, and their well known stability, complicate MA...

journal_title：FEMS microbiology letters

authors： Balážová T,Makovcová J,Šedo O,Slaný M,Faldyna M,Zdráhal Z

更新日期：2014-04-01 00:00:00

abstract：:Fusarium graminearum was grown on four lignocellulosic substrates (corn cobs, wheat bran, hop cell walls, and birchwood) and glucose as the sole carbon source. Proteomic studies performed on the resulting enzymatic cocktails highlighted a great diversity in the number and type of proteins secreted. The cell wall-degra...

journal_title：FEMS microbiology letters

authors： Debeire P,Delalande F,Habrylo O,Jeltsch JM,Van Dorsselaer A,Phalip V

更新日期：2014-06-01 00:00:00

abstract：:Within the phylum Bacteroidetes, the gyrB gene, encoding for the B subunit of the DNA gyrase, has been used as a phylogenetic marker for several genera closely related to Flavobacterium. The phylogenies of the complete 16S rRNA gene and the gyrB gene were compared for 33 Antarctic Flavobacterium isolates and 23 type s...

journal_title：FEMS microbiology letters

authors： Peeters K,Willems A

更新日期：2011-08-01 00:00:00

abstract：:This study focused on the alanine racemase gene (alr-2), which is involved in the synthesis of d-alanine that forms the backbone of the cell wall. A stable alr-2 knockout mutant of Aeromonas hydrophila HBNUAh01 was constructed. When the mutant was supplemented with d-alanine, growth was unaffected; deprivation of d-al...

journal_title：FEMS microbiology letters

authors： Liu D,Zhang L,Xue W,Wang Y,Ju J,Zhao B

更新日期：2015-07-01 00:00:00

abstract：:Enteroaggregative Escherichia coli (EAEC) is an important diarrhoeal pathogen causing diseases in multiple epidemiological and clinical settings. In developing countries like India, diarrhoeal diseases are one of the major killers among paediatric population and oddly, few studies are available from Indian paediatric ...

journal_title：FEMS microbiology letters

authors： Gupta D,Sharma M,Sarkar S,Thapa BR,Chakraborti A

更新日期：2016-09-01 00:00:00

abstract：:In Bordetella pertussis virulence-associated genes, including adenylate cyclase toxin (Cya), are coordinately regulated in response to environmental signals by proteins coded by the bvg-locus. We have constructed cya-lac fusions in Escherichia coli and have shown that the cya operon is not expressed in E. coli, neithe...

journal_title：FEMS microbiology letters

authors： Goyard S,Ullmann A

更新日期：1991-01-15 00:00:00

abstract：:Environmental persistence of Mycobacterium tuberculosis is subject to speculation. However, the reality that infected postmortem tissues can be a danger to pathologists and embalmers has worrisome implications. A few experimental studies have demonstrated the organism's ability to withstand exposure to embalming fluid...

journal_title：FEMS microbiology letters

authors： Lemma E,Zimhony O,Greenblatt CL,Koltunov V,Zylber MI,Vernon K,Spigelman M

更新日期：2008-06-01 00:00:00

abstract：:The identification of virulence factors in Rhodococcus equi has been severely hampered by the lack of a method for in vivo random insertion mutagenesis. This study reports the use of transposomes to generate random insertions of a gene conferring kanamycin resistance into the genome of R. equi ATCC 33701. Southern hyb...

journal_title：FEMS microbiology letters

authors： Mangan MW,Meijer WG

更新日期：2001-12-18 00:00:00

abstract：:Agrobacterium tumefaciens is the causal agent of crown gall disease and is a vector for DNA transfer in transgenic plants. The transformation process by A. tumefaciens has been widely studied, but the attachment stage has not been well characterized. Most measurements of attachment have used microscopy and colony coun...

journal_title：FEMS microbiology letters

authors： Petrovicheva A,Joyner J,Muth TR

更新日期：2017-10-02 00:00:00

abstract：:A physical and functional map of Aspergillus tubingensis mtDNA type 2b was constructed and compared to Aspergillus niger typeI a mtDNA. The gene content and order, as well as the patterns of restriction sites, were similar. Two unidentified ORFs and several repeat elements were found in the region between the ndhl and...

journal_title：FEMS microbiology letters

authors： Juhász A,Láday M,Gácser A,Kucsera J,Pfeiffer I,Kevei F,Hamari Z

更新日期：2004-12-01 00:00:00

abstract：:The nitrogen-fixing cyanobacterium Nostoc sp. ATCC 29132 was shown to contain two sequence-specific endonucleases. Nsp(29132) I was an isoschizomer of AsuII, and Nsp(29132) II was an isoschizomer of BamHI. Nsp(29132) II was shown to generate ends that could be ligated to those generated by BamHI. ...

journal_title：FEMS microbiology letters

authors： Muro-Pastor AM,Herrero A,Flores E

更新日期：1991-01-01 00:00:00

abstract：:As part of the ongoing sequencing of the complete Salmonella typhimurium LT2 genome, a partly ordered set of 416 lambda clones has been developed, representing over 90% of the genome. The average insert size is 17 kb. Sequences were obtained from both ends of each clone in this set. A total of over 600 kb of sequence ...

journal_title：FEMS microbiology letters

authors： Wong RM,Wong KK,Benson NR,McClelland M

更新日期：1999-04-15 00:00:00