Tetratricopeptide repeats are essential for PcrH chaperone function in Pseudomonas aeruginosa type III secretion.

abstract：:Trichothecenes are an important family of mycotoxins produced by several species of the genus Fusarium. These fungi cause serious disease on infected plants and postharvest storage of crops, and the toxins can cause health problems for humans and animals. Unfortunately, there are few methods for controlling mycotoxin ...

journal_title：FEMS microbiology letters

authors： Ismail Y,McCormick S,Hijri M

更新日期：2013-11-01 00:00:00

abstract：:The microbial adhesion to hydrocarbons (MATH) test is one of the most common method to determine the hydrophobicity of cell surfaces. Despite its prevalence, no standard test parameters are used in literature, making a direct comparison of data almost impossible. Criticism also focuses on test parameters that may mask...

journal_title：FEMS microbiology letters

authors： Nachtigall C,Weber C,Rothenburger S,Jaros D,Rohm H

更新日期：2019-06-01 00:00:00

abstract：:An accurate monitoring of disease progression is important to evaluate disease susceptibility phenotypes. Over the years, Arabidopsis thaliana has become the model species to serve as a host in plant-pathogen interactions. Despite the efforts to study genetic mechanisms of host defense, little efforts are made for a t...

journal_title：FEMS microbiology letters

authors： Brouwer M,Lievens B,Van Hemelrijck W,Van den Ackerveken G,Cammue BP,Thomma BP

更新日期：2003-11-21 00:00:00

abstract：:Saccharomyces cerevisiae exponentially growing in basic or 0.7 M NaCl medium were isotopically labelled with 35S-methionine, followed by protein separation and quantification by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) combined with computerised image analysis. The electrophoretic separation resolv...

journal_title：FEMS microbiology letters

authors： Norbeck J,Blomberg A

更新日期：1996-03-15 00:00:00

abstract：:Agrobacterium tumefaciens strain SCUEC1 is a nicotine-degrading bacterium, which has been recently isolated from the tobacco waste-contaminated field soil. However, the mechanism for nicotine degradation in this strain remains unclear. Here, we analyze the function and biological properties of the agnH gene in the str...

journal_title：FEMS microbiology letters

authors： Xia Z,Yu M,Yao J,Feng Z,Li D,Liu T,Cheng G,He D,Li X

更新日期：2020-02-01 00:00:00

abstract：:A Caulobacter crescentus rho::Tn5 mutant strain presenting a partially functional transcription termination factor Rho is highly sensitive to hydrogen peroxide in both exponential and stationary phases. The mutant was shown to be permanently under oxidative stress, based on fluorophore oxidation, and also to be sensit...

journal_title：FEMS microbiology letters

authors： Italiani VC,Braz VS,Xiao H,Steinman HM,Marques MV

更新日期：2010-02-01 00:00:00

abstract：:Two different nucleic acid precursor utilization patterns were obtained for five avian isolates of Chlamydia psittaci. Three of the isolates behaved in a manner similar to that previously described, showing total dependency on the host cell for ribonucleoside triphosphates and being unable to utilize medium-supplied t...

journal_title：FEMS microbiology letters

authors： McClarty G,Fan H,Andersen AA

更新日期：1993-04-15 00:00:00

abstract：:Acinetobacter baumannii strain A148, a clinical isolate resistant to imipenem (MIC = 32 mg l-1), synthesized two beta-lactamases with pIs 6.3 and > 9.2. The pI 6.3 enzyme hydrolyzed the penicillins, including isoxazoylpenicillins, first-, second- and, to a lesser extent, third-generation cephalosporins. It was inhibit...

journal_title：FEMS microbiology letters

authors： Hornstein M,Sautjeau-Rostoker C,Péduzzi J,Vessières A,Hong LT,Barthélémy M,Scavizzi M,Labia R

更新日期：1997-08-15 00:00:00

abstract：:Although very little, if any, beta-galactosidase activity is detected in Yersinia pestis by a standard Miller assay, we found that Y. pestis KIM6+ cells formed blue colonies on plates containing 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal). Searches of the Y. pestis genome databases revealed the presence of n...

journal_title：FEMS microbiology letters

authors： Bobrov AG,Perry RD

更新日期：2006-02-01 00:00:00

abstract：:Pneumocystis carinii pneumonia was induced in rats by chronic corticosteroid immunosuppression. The parasite was separated from virtually all contaminating host cells using the technique of unit gravity sedimentation. Cellular contamination was routinely below 0.02%. The same technique allowed partial separation of th...

journal_title：FEMS microbiology letters

authors： Taylor MB,Easmon CS

更新日期：1990-06-15 00:00:00

abstract：:The growing demand to replace fossil fuels with renewable alternatives has generated an urgent and imminent global need to find new non-fossil sources. Sweet sorghum is widely recognized as a highly promising biomass energy crop with the particular potential to complement sugarcane for ethanol production. Our aim in t...

journal_title：FEMS microbiology letters

authors： Durán MA,Dantur K,Ruiz RM,Romero ER,Zossi S,Gusils C

更新日期：2018-07-01 00:00:00

abstract：:Mycoplasma mycoides subsp. mycoides (Mmm) strain Afadé had previously been shown to undergo spontaneous phase variations between an opaque capsulated variant and a translucent (TR) variant devoid of a capsule but able to secrete cell-free exopolysaccharides. This phase variation is associated with an ON/OFF genetic sw...

journal_title：FEMS microbiology letters

authors： Gaurivaud P,Lakhdar L,Le Grand D,Poumarat F,Tardy F

更新日期：2014-10-01 00:00:00

abstract：:Rhamnolipids are biosurfactants produced by the soil bacterium P seudomonas aeruginosa. In addition to their high industrial potential as surface-active molecules, rhamnolipids also have antimicrobial properties. In densely populated habitats, such as the soil, production of antimicrobial compounds is important to inh...

journal_title：FEMS microbiology letters

authors： Wecke T,Bauer T,Harth H,Mäder U,Mascher T

更新日期：2011-10-01 00:00:00

abstract：:A lysC-lac'Z fusion plasmid was constructed to study the regulatory region of the lysC gene. Analysis by deletion mutations confirmed the existence of an alternative promoter, P2, located upstream of the previously identified promoter, P1. The transcription start site of promoter P2 was located 85 base pairs upstream ...

journal_title：FEMS microbiology letters

authors： Liao HH,Hseu TH

更新日期：1998-11-01 00:00:00

abstract：:A 1.8-kb cryptic plasmid pFR18 was isolated from Leuconostoc mesenteroides ssp. mesenteroides FR52 and characterized. The identification of single-stranded DNA intermediate (ssDNA) in Leuconostoc demonstrated that the replication of pFR18 is directed by a rolling-circle mechanism (RCR). Sequence analysis revealed a si...

journal_title：FEMS microbiology letters

authors： Biet F,Cenatiempo Y,Fremaux C

更新日期：1999-10-15 00:00:00

abstract：:A total of 401 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates from two experimentally infected calves were analyzed using molecular biological methods. Genetic differences detected by pulsed-field gel electrophoresis were observed between the inoculated and recovered strains as early as 1 day post inoculat...

journal_title：FEMS microbiology letters

authors： Akiba M,Sameshima T,Nakazawa M

更新日期：2000-03-01 00:00:00

abstract：:We adapted PCR-based signature-tagged mutagenesis (STM) to Pseudomonas aeruginosa. A collection of 1056 mutants was screened in a chronic lung infection rat model. Thirteen mutants were confirmed to be attenuated. Analysis revealed that these STM mutants represented transposon insertions into eight genes previously de...

journal_title：FEMS microbiology letters

authors： Lehoux DE,Sanschagrin F,Levesque RC

更新日期：2002-04-23 00:00:00

abstract：:Salmonella enteritidis isolated from poultry infections generated a convoluted colonial morphology after 48 h growth on colonisation factor antigen (CFA) agar at 25 degrees C. A mutant S. enteritidis defective for the elaboration of the SEF17 fimbrial antigen, in which the agf gene cluster was inactivated by insertion...

journal_title：FEMS microbiology letters

authors： Allen-Vercoe E,Dibb-Fuller M,Thorns CJ,Woodward MJ

更新日期：1997-08-01 00:00:00

abstract：:We have analyzed 81 isolates of Mycoplasma agalactiae from four different regions of Italy between 1990 and 1995 in order to identify antigenic differences through SDS-PAGE and Western blotting and chromosomal DNA restriction endonuclease cleavage pattern differences. Antigenic variability in M. agalactiae isolates wa...

journal_title：FEMS microbiology letters

authors： Tola S,Idini G,Manunta D,Casciano I,Rocchigiani AM,Angioi A,Leori G

更新日期：1996-10-01 00:00:00

abstract：:Soil isolate Pseudomonas sp. strain AKN5 degrades atrazine as the sole source of nitrogen. The strain showed expeditious growth on medium containing citrate as the carbon source and ammonium chloride as the nitrogen source as compared to citrate plus atrazine or cyanuric acid. Biochemical and nitrogen-source-dependent...

journal_title：FEMS microbiology letters

authors： Sharma A,Kalyani P,Trivedi VD,Kapley A,Phale PS

更新日期：2019-01-01 00:00:00

abstract：:A plasmid-based food-grade vector system was developed for Lactococcus lactis by exploiting the genes for lactose metabolism. L. lactis MG5267 is a plasmid-free strain containing the entire lactose operon as a chromosomal insertion. The lacF gene was deleted from this strain by a double cross-over homologous recombina...

journal_title：FEMS microbiology letters

authors： MacCormick CA,Griffin HG,Gasson MJ

更新日期：1995-03-15 00:00:00

abstract：:From the nisZ gene sequence, a non-radioactive digoxigenin-labeled DNA probe, was tested for detection of nisin-producing strains using polymerase chain reaction amplification. The digoxigenin-labeled DNA probe clearly discriminated between nisin-producing and non-producing strains with a high degree of sensitivity an...

journal_title：FEMS microbiology letters

authors： Meghrous J,Fliss I,Bouksaim M,Lacroix C

更新日期：1999-02-01 00:00:00

abstract：:Abstract The emergence of the nontuberculosis mycobacteria (NTM) as clinically relevant pathogens has warranted the study of these ubiquitous organisms in the context of their likely environmental niche, the biofilm. We assayed the NTM bacterium Mycobacterium marinum strain 1218R, a fish outbreak isolate, for biofilm ...

journal_title：FEMS microbiology letters

authors： Hall-Stoodley L,Brun OS,Polshyna G,Barker LP

更新日期：2006-04-01 00:00:00

abstract：:Multidrug efflux pumps contribute to multiple antibiotic resistance in Pseudomonas aeruginosa. Pump expression usually has been quantified by Western blotting. Quantitative real-time polymerase chain reaction has been developed to measure mRNA expression for genes of interest. Whether this method correlates with pump ...

journal_title：FEMS microbiology letters

authors： Yoneda K,Chikumi H,Murata T,Gotoh N,Yamamoto H,Fujiwara H,Nishino T,Shimizu E

更新日期：2005-02-01 00:00:00

abstract：:Two clones, obtained by polymerase chain reaction from macronuclear DNA of the unicellular ciliated protist Blepharisma japonicum, were isolated and sequenced. They correspond to fragments of two different putative H4 histone genes. The existence of multiple H4 histone genes was also suggested by Southern blot hybridi...

journal_title：FEMS microbiology letters

authors： Salvini M,Bini E,Santucci A,Batistoni R

更新日期：1997-04-01 00:00:00

abstract：:Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) represents a simple reliable approach for rapid bacterial identification based on specific peptide/protein fingerprints. However, cell-wall characteristics of mycobacterial species, and their well known stability, complicate MA...

journal_title：FEMS microbiology letters

authors： Balážová T,Makovcová J,Šedo O,Slaný M,Faldyna M,Zdráhal Z

更新日期：2014-04-01 00:00:00

abstract：:Using labelled, gamma-32P rRNA of mycobacteria as a probe restriction fragment length polymorphism (RFLP) of rRNA genes of strains belonging to the Mycobacterium fortuitum-chelonei complex was analysed. Each DNA sample was cleaved with EcoRI restriction endonuclease, the fragments were separated by agarose gel electro...

journal_title：FEMS microbiology letters

authors： Kanaujia GV,Katoch VM,Shivannavar CT,Sharma VD,Patil MA

更新日期：1991-01-15 00:00:00

abstract：:The denitrifying bacterium Thauera aromatica strain AR-1 grows anaerobically with protocatechuate (3,4-dihydroxybenzoate (DHB)) as sole energy and carbon source. This bacterium harbors two distinct pathways for degradation of aromatic compounds, the benzoyl-coenzyme A (CoA) pathway for benzoate degradation and the hyd...

journal_title：FEMS microbiology letters

authors： Philipp B,Kemmler D,Hellstern J,Gorny N,Caballero A,Schink B

更新日期：2002-06-18 00:00:00

abstract：:The major heat shock proteins from Thiobacillus ferrooxidans were identified as DnaK and GroEL equivalents by Western blotting and analysis of the N-terminal amino acid sequence of spots isolated from dried 2-D polyacrylamide electrophoresis gels. The T. ferrooxidans chaperonins showed 70% and 80% identity with the Es...

journal_title：FEMS microbiology letters

authors： Varela P,Jerez CA

更新日期：1992-11-01 00:00:00

abstract：:A particular property of Clostridium difficile strain C34 is an insertion of approximately 2 kb in the tcdA-C34 gene that does not hinder expression of a fully active TcdA-C34 molecule. Intoxication with TcdA-C34 induced an arborized appearance in eukaryotic cells (D-type cytopathic effect); intoxication with TcdB-C34...

journal_title：FEMS microbiology letters

authors： Mehlig M,Moos M,Braun V,Kalt B,Mahony DE,von Eichel-Streiber C

更新日期：2001-05-01 00:00:00