Microorganisms form exocellular structures, trophosomes, to facilitate biodegradation of oil in aqueous media.

Abstract:

:Cytochemical staining and microscopy were used to study the trophic structures and cellular morphotypes that are produced during the colonization of oil-water interfaces by oil-degrading yeasts and bacteria. Among the microorganisms studied here, the yeasts (Schwanniomyces occidentalis, Torulopsis candida, Candida tropicalis, Candida lipolytica, Candida maltosa, Candida paralipolytica) and two representative bacteria (Rhodococcus sp. and Pseudomonas putida) produced exocellular structures composed of biopolymers during growth on petroleum hydrocarbons. Four of the yeasts including S. occidentalis, T. candida, C. tropicalis and C. maltosa excreted polymers through modified sites in their cell wall ('canals'), whereas C. lipolytica and C. paralipolytica and the two bacterial species secreted polymers over the entire cell surface. These polymers took the form of fibrils and films that clogged pores and cavities on the surfaces of the oil droplets. A three-dimensional reconstruction of the cavities using serial thin sections showed that the exopolymer films isolated the ambient aqueous medium together with microbial cells and oil to form both closed and open granules that contained pools of oxidative enzymes utilized for the degradation of the oil hydrocarbons. The formation of such granules, or 'trophosomes,' appears to be a fundamental process that facilitates the efficient degradation of oil in aqueous media.

journal_name

FEMS Microbiol Lett

authors

Dmitriev VV,Crowley D,Rogachevsky VV,Negri CM,Rusakova TG,Kolesnikova SA,Akhmetov LI

doi

10.1111/j.1574-6968.2010.02184.x

subject

Has Abstract

pub_date

2011-02-01 00:00:00

pages

134-40

issue

2

eissn

0378-1097

issn

1574-6968

journal_volume

315

pub_type

杂志文章
  • Adenosine kinase-deficient mutant of Saccharomyces cerevisiae.

    abstract::A cordycepin-resistant mutant strain of Saccharomyces cerevisiae (CD-R2) was found to be deficient in adenosine kinase. This mutant accumulated S-adenosylhomocysteine during growth in the presence of exogenous adenosine and it grew in a pseudohyphal manner in the presence of this nucleotide. ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07444.x

    authors: Iwashima A,Ogata M,Nosaka K,Nishimura H,Hasegawa T

    更新日期:1995-03-15 00:00:00

  • Evaluation of the catalase promoter for expressing the alkaline xylanase gene (alx) in Aspergillus niger.

    abstract::Aspergillus niger represents a promising host for the expression of recombinant proteins, but only a few expression systems are available for this organism. In this study, the inducible catalase promoter (PcatR) from A. niger was characterized. For this, constructs were developed and checked for the expression of the ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02454.x

    authors: Sharma R,Katoch M,Govindappa N,Srivastava PS,Sastry KN,Qazi GN

    更新日期:2012-02-01 00:00:00

  • IdeE, an IgG-endopeptidase of Streptococcus equi ssp. equi.

    abstract::Streptococcus equi ssp. equi is the causative agent of strangles, a highly contagious and serious disease in the upper respiratory tract of horses. The present study describes the characterization of IdeE, a homolog of the secreted IgG-specific protease IdeS/Mac of Streptococcus pyogenes. The activity of IdeE is compa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00404.x

    authors: Lannergård J,Guss B

    更新日期:2006-09-01 00:00:00

  • Sterols in a psychrophilic methanotroph, Methylosphaera hansonii.

    abstract::A suite of six sterols, lanosterol, lanost-8(9)-en-3beta-ol, 4, 4-dimethylcholesta-8(14),24-dien-3beta-ol, 4, 4-dimethylcholest-8(14)-en-3beta-ol, 4-methylcholesta-8(14), 24-dien-3beta-ol and 4-methylcholest-8(14)-en-3beta-ol, were identified in the psychrophilic methanotrophic bacterium, Methylosphaera hansonii. Thei...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09103.x

    authors: Schouten S,Bowman JP,Rijpstra WI,Sinninghe Damsté JS

    更新日期:2000-05-15 00:00:00

  • Search for additional targets of the transcriptional regulator CcpN from Bacillus subtilis.

    abstract::The transcriptional repressor CcpN from Bacillus subtilis mediates the CcpA-independent catabolite repression of three genes, sr1, encoding a small regulatory RNA, and two gluconeogenesis genes, gapB and pckA. The intracellular concentration of CcpN was determined to be around 4000 molecules per cell. The B. subtilis ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01754.x

    authors: Eckart RA,Brantl S,Licht A

    更新日期:2009-10-01 00:00:00

  • Characterization of a novel plasmid from extremely halophilic Archaea: nucleotide sequence and function analysis.

    abstract::We determined the complete nucleotide sequence of the 16341 bp plasmid pHH205 of the extremely halophilic archaeon Halobacterium salinarum J7. The plasmid has a G+C content of 61.1%. A number of direct and inverted repeat sequences were found in pHH205, while no insertion sequences were found. Thirty-eight large open ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00175-7

    authors: Ye X,Ou J,Ni L,Shi W,Shen P

    更新日期:2003-04-11 00:00:00

  • Rhizobium leguminosarum bv. trifolii rosR gene expression is regulated by catabolic repression.

    abstract::Rhizobium leguminosarum bv. trifolii rosR gene encodes a transcriptional regulator involved in the positive regulation of exopolysaccharide synthesis. Transcription of rosR is directed by two promoters, distal P1 and proximal P2, of different strengths. We demonstrated that rosR P1 functions as the main promoter and, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01443.x

    authors: Janczarek M,Skorupska A

    更新日期:2009-02-01 00:00:00

  • Purification and characteristics of cytosolic chitinase from Piromyces communis OTS1.

    abstract::A chitinase was purified from the cytosolic fraction of the anaerobic rumen fungus Piromyces communis OTS1 by affinity chromatography using regenerated chitin, gel filtration and chromatofocusing. The chitinase was most active at pH 6.2 and at 60 degrees C in a 20-min assay. The molecular mass of the purified protein ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08085.x

    authors: Sakurada M,Morgavi DP,Komatani K,Tomita Y,Onodera R

    更新日期:1996-03-15 00:00:00

  • Evidence for importance of the Staphylococcus hyicus lipase pro-peptide in lipase secretion, stability and activity.

    abstract::To investigate the function of the pro-peptide (PP) region of the Staphylococcus hyicus exolipase, restriction sites were created in the lipase gene to facilitate the construction of deletions in this region. Lipase gene expression was carried out in Staphylococcus carnosus. In the presence of the entire PP region, th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb07098.x

    authors: Demleitner G,Götz F

    更新日期:1994-08-15 00:00:00

  • Variant toxin B and a functional toxin A produced by Clostridium difficile C34.

    abstract::A particular property of Clostridium difficile strain C34 is an insertion of approximately 2 kb in the tcdA-C34 gene that does not hinder expression of a fully active TcdA-C34 molecule. Intoxication with TcdA-C34 induced an arborized appearance in eukaryotic cells (D-type cytopathic effect); intoxication with TcdB-C34...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10638.x

    authors: Mehlig M,Moos M,Braun V,Kalt B,Mahony DE,von Eichel-Streiber C

    更新日期:2001-05-01 00:00:00

  • Green fluorescent protein--a bright idea for the study of bacterial protein localization.

    abstract::Use of the green fluorescent protein (GFP) of Aequorea victoria as a reporter for protein and DNA localization has provided sensitive, new approaches for studying the organization of the bacterial cell, leading to new insights into diverse cellular processes. GFP has many characteristics that make it useful for locali...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.2001.tb10854.x

    authors: Phillips GJ

    更新日期:2001-10-16 00:00:00

  • Differential expression of proteolytic enzymes in endosymbiont-harboring Crithidia species.

    abstract::Crithidia oncopelti, Crithidia deanei and Crithidia desouzai are flagellates of the Trypanosomatidae family that present bacterium-like endosymbionts in their cytoplasm. Gelatin-SDS-PAGE analysis was used to characterize cell-associated and extracellular proteinases in these organisms. Our survey indicates that the pr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10782.x

    authors: d'Avila-Levy CM,Melo AC,Vermelho AB,Branquinha MH

    更新日期:2001-08-07 00:00:00

  • Functional expression of the glutamate uptake system from Corynebacterium glutamicum in Escherichia coli.

    abstract::Glutamate uptake in the Gram-positive Corynebacterium glutamicum is mediated via a binding protein-dependent transport system, which is encoded by the gluABCD gene cluster. Cloning of these genes in an expression vector and subsequent transformation of the resulting plasmid allows different strains of the Gram-negativ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07483.x

    authors: Burkovski A,Krämer R

    更新日期:1995-04-01 00:00:00

  • Prevotella intermedia induces matrix metalloproteinase-9 expression in human periodontal ligament cells.

    abstract::Matrix metalloproteinases (MMPs) play pivotal roles in inflammatory diseases including chronic periodontitis. The effects of Prevotella intermedia, a major periodontal pathogen, on MMP-9 production in primary human periodontal ligament (hPDL) cells were examined in the present study. MMP-9 mRNA expression was measured...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01140.x

    authors: Guan SM,Shu L,Fu SM,Liu B,Xu XL,Wu JZ

    更新日期:2008-06-01 00:00:00

  • Mitochondrial DNA organisation of the mtDNA type 2b of Aspergillus tubingensis compared to the Aspergillus niger mtDNA type 1a.

    abstract::A physical and functional map of Aspergillus tubingensis mtDNA type 2b was constructed and compared to Aspergillus niger typeI a mtDNA. The gene content and order, as well as the patterns of restriction sites, were similar. Two unidentified ORFs and several repeat elements were found in the region between the ndhl and...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.10.025

    authors: Juhász A,Láday M,Gácser A,Kucsera J,Pfeiffer I,Kevei F,Hamari Z

    更新日期:2004-12-01 00:00:00

  • The emergence of colistin-resistant Escherichia coli in chicken meats in Nepal.

    abstract::The emergence and dissemination of colistin resistance among Gram-negative bacteria is a global problem. We initiated a surveillance of colistin-resistant and -susceptible Escherichia coli in raw meats from chicken in Nepal. A total of 180 meat samples were collected; from these, 60 E. coli strains were isolated (33.3...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz237

    authors: Joshi PR,Thummeepak R,Leungtongkam U,Pooarlai R,Paudel S,Acharya M,Dhital S,Sitthisak S

    更新日期:2019-10-01 00:00:00

  • Cloning and expression in Escherichia coli of dextranase genes from Bacteroides thetaiotaomicron.

    abstract::A genomic bank was constructed in Escherichia coli HB101, consisting of DNA fragments from Bacteroides thetaiotaomicron strain 489 inserted within the vector pBR322. By screening on complex medium containing blue dextran, 10 stable dextranase-positive (Dex+) clones were isolated. Seven groups of Dex+ inserts were iden...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(91)90368-k

    authors: Joncquiert JC,Béchet M,Tierny Y,Courtois J,Dubourguier HC,Guillaume JB

    更新日期:1991-12-01 00:00:00

  • Characterization of a replication locus and formation of a higher-order complex between RepA protein and two inverted repeats in Streptomyces plasmid pSV1.

    abstract::We identified the minimal locus of 163-kb plasmid pSV1 of Streptomyces violaceoruber for the replication in S. lividans. This locus comprised a repA gene and an upstream 407-bp sequence containing two inverted repeats (IR-III and IR-IV) within an iteron, an AT-rich region and a 300-bp noncoding sequence (NCS). RepA pr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12307

    authors: Chen Y,Tan H,Qin Z

    更新日期:2013-12-01 00:00:00

  • Plasmid-mediated virulence genes in non-typhoid Salmonella serovars.

    abstract::Specific non-typhoid Salmonella serovars carry large virulence plasmids that promote sustained extra-intestinal infections. These plasmids all share a highly conserved 8-kb region containing the spv operon, consisting of the regulatory spvR locus and the four structural spvABCD genes. The SpvR protein belongs to the L...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.1994.tb07253.x

    authors: Guiney DG,Fang FC,Krause M,Libby S

    更新日期:1994-11-15 00:00:00

  • Evaluation of T3B fingerprinting for identification of clinical and environmental Sporothrix species.

    abstract::In this study, PCR fingerprinting using the universal primer T3B was applied to distinguish among clinical and environmental species of the Sporothrix complex, Sporothrix brasiliensis, S. globosa, S. mexicana, S. pallida, S. luriei and S. schenckii sensu stricto. The T3B fingerprinting generated clearly distinct bandi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv027

    authors: Oliveira MM,Franco-Duarte R,Romeo O,Pais C,Criseo G,Sampaio P,Zancope-Oliveira RM

    更新日期:2015-03-01 00:00:00

  • Characterization of IS1541-like elements in Yersinia enterocolitica and Yersinia pseudotuberculosis.

    abstract::We characterized Yersinia enterocolitica and Yersinia pseudotuberculosis insertion sequences related to insertion sequence 1541, recently identified in Yersinia pestis. For each of the two species, two insertion sequence copies were cloned and sequenced. Genetic elements from Y. pseudotuberculosis were almost identica...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13666.x

    authors: Devalckenaere A,Odaert M,Trieu-Cuot P,Simonet M

    更新日期:1999-07-01 00:00:00

  • The pel1 mutant of Saccharomyces cerevisiae is deficient in cardiolipin and does not survive the disruption of the CHO1 gene encoding phosphatidylserine synthase.

    abstract::Cells of the pel1 mutant of Saccharomyces cerevisiae were found to contain an extremely low content of cardiolipin, a decreased level of phosphatidylcholine and an increased level of phosphatidylinositol. Disruption of the PEL1 gene in cells containing a null mutation in the CHO1 gene was lethal. Despite its putative ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08312.x

    authors: Janitor M,Obernauerová M,Kohlwein SD,Subík J

    更新日期:1996-06-15 00:00:00

  • Kinetic and substrate complex characterization of RamA, a corrinoid protein reductive activase from Methanosarcina barkeri.

    abstract::In microbial corrinoid-dependent methyltransferase systems, adventitious Co(I)-corrinoid oxidation halts catalysis and necessitates repair by ATP-dependent reductive activases. RamA, an activase with a C-terminal ferredoxin domain with two [4Fe-4S] clusters from methanogenic archaea, has been far less studied than the...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnaa128

    authors: Huening KA,Jiang R,Krzycki JA

    更新日期:2020-09-01 00:00:00

  • Synthesis of poly(3-hydroxyalkanoates) in Escherichia coli expressing the PHA synthase gene phaC2 from Pseudomonas aeruginosa: comparison of PhaC1 and PhaC2.

    abstract::In order to obtain functional expression of PHA synthase gene phaC2 from Pseudomonas aeruginosa in Escherichia coli, the coding region of phaC2 was subcloned, including the ribosomal binding site, into pBluescript SK- collinear to the lac promoter. This plasmid pBHR71-C2 enabled functional expression of phaC2 in E. co...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb12767.x

    authors: Qi Q,Rehm BH,Steinbüchel A

    更新日期:1997-12-01 00:00:00

  • Metabolic flux response to phosphoglucose isomerase knock-out in Escherichia coli and impact of overexpression of the soluble transhydrogenase UdhA.

    abstract::Blocking glycolytic breakdown of glucose by inactivation of phosphoglucose isomerase (Pgi) in Escherichia coli led to a greatly reduced maximum specific growth rate. Examination of the operational catabolic pathways and their flux ratios using [U-(13)C(6)]glucose-labeling experiments and metabolic flux ratio analysis ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10892.x

    authors: Canonaco F,Hess TA,Heri S,Wang T,Szyperski T,Sauer U

    更新日期:2001-11-13 00:00:00

  • Inactivation of the fis gene leads to reduced growth rate.

    abstract::The FIS protein (factor for inversion stimulation) is known to activate the transcription of rRNA and tRNA operons in Escherichia coli. In this report the growth behaviour of a fis bacterium was studied under steady-state conditions and in competition experiments with a wild-type bacterium in a chemostat. The growth r...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90292-v

    authors: Nilsson L,Verbeek H,Hoffmann U,Haupt M,Bosch L

    更新日期:1992-11-15 00:00:00

  • Comparison of five rep-PCR genomic fingerprinting methods for differentiation of fecal Escherichia coli from humans, poultry and wild birds.

    abstract::The development of a methodology to identify the origin of fecal pollution is important both for assessing the degree of risk posed to public health and for developing strategies to mitigate the environmental loading of pathogens associated with waterborne disease transmission. Five rep-PCR genomic fingerprinting meth...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00948.x

    authors: Mohapatra BR,Broersma K,Mazumder A

    更新日期:2007-12-01 00:00:00

  • Two types of ion channel formation of tolaasin, a Pseudomonas peptide toxin.

    abstract::Tolaasin is a peptide toxin produced by Pseudomonas tolaasii and causes brown blotch disease of the cultivated mushrooms. Two types of ion channels were identified by the incorporation of tolaasin into lipid bilayer. The slope conductance of type 1 channel measured in the buffer containing 100 mM KCl was 150 pS with a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00182-4

    authors: Cho KH,Kim YK

    更新日期:2003-04-25 00:00:00

  • Activation of FNR-dependent transcription by iron: an in vitro switch for FNR.

    abstract::FNR is an iron-binding transcriptional regulator of Escherichia coli which controls the expression of target genes in response to anaerobiosis. The mechanism used by FNR to sense and respond to anaerobiosis is unknown but it is thought to involve iron. In vitro transcription analyses have shown that iron-deficient FNR...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06517.x

    authors: Green J,Guest JR

    更新日期:1993-10-15 00:00:00

  • Identification of genes transcribed by Pasteurella multocida in rabbit livers through the selective capture of transcribed sequences.

    abstract::Pasteurella multocida, a Gram-negative nonmotile coccobacillus, is the causative agent of fowl cholera in poultry, hemorrhagic septicemia in cattle, atropic rhinitis in swine, and snuffles in rabbits. The differentially expressed gene profile of P. multocida in infected rabbit livers was identified and compared with t...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2012.02559.x

    authors: Guo D,Lu Y,Zhang A,Liu J,Yuan D,Jiang Q,Lin H,Si C,Qu L

    更新日期:2012-06-01 00:00:00