Neuraminidase (sialidase) activity of Haemophilus parasuis.

Abstract:

:Neuraminidase (sialidase), a potential virulence factor in bacteria, was demonstrated in Haemophilus parasuis, an invasive swine pathogen, but not in four other pathogens of the Pasteurellaceae family: H. influenzae, H. somnus, H. paragallinarum, or Actinobacillus pleuropneumoniae. H. parasuis neuraminidase had an acidic pH optimum and a specificity for several substrates also cleaved by other bacterial neuraminidases. Similar to the neuraminidase of Pasteurella multocida, H. parasuis neuraminidase was cell associated and did not require divalent cations for activity. Exogenous sialic acid added to growth medium of H. parasuis was cleared after a lag of about 10 h and these cultures grew to a greater final density than cultures without added sialic acid, indicating that exogenous sialic acid is metabolized. The role of sialidase in providing nutrients to H. parasuis may be an important factor in its obligate parasitism.

journal_name

FEMS Microbiol Lett

authors

Lichtensteiger CA,Vimr ER

doi

10.1111/j.1574-6968.1997.tb10438.x

subject

Has Abstract

pub_date

1997-07-15 00:00:00

pages

269-74

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(97)00209-7

journal_volume

152

pub_type

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