Binding and hemagglutinating properties of the B subunit(s) of heat-labile enterotoxin isolated from human enterotoxigenic Escherichia coli.

abstract：:A cloning cassette, carrying a modified amino-glycoside resistance gene (neo) from transposon Tn5 was constructed. Three restriction sites internal to the neo gene were eliminated by in vitro mutagenesis, allowing their use in designing new cloning vectors. The original, suboptimal transcription promoter was replaced ...

journal_title：FEMS microbiology letters

authors： Denis F,Brzezinski R

更新日期：1991-07-01 00:00:00

abstract：:Haemophilus parasuis, the etiological agent of Glässer's disease in pigs, possesses iron acquisition pathways mediated by a surface receptor that specifically bind porcine transferrin. This receptor is composed of transferrin-binding protein A (TbpA) and TbpB. As it has been reported for other gram-negative organisms,...

journal_title：FEMS microbiology letters

authors： Martínez S,Frandoloso R,Rodríguez-Ferri EF,González-Zorn B,Gutiérrez-Martín CB

更新日期：2010-06-01 00:00:00

abstract：:FlgM inhibits the flagella-specific sigma factor FliA and is involved in the mouse-virulence of Salmonella typhimurium. In recent experiments, we observed that: (i) a flgM gene that could function to negatively regulate flagella synthesis was present in a variety of salmonellae; and (ii) the flgM gene derived from Sal...

journal_title：FEMS microbiology letters

authors： Schmitt CK,Darnell SC,O'Brien AD

更新日期：1996-01-15 00:00:00

abstract：:beta-Glucosidase in Aspergillus nidulans was found to be both intracellular and extracellular. The intracellular beta-glucosidase was synthesized after the exhaustion of carbon source in the medium. The extracellular enzyme appeared with autolysis of the mycelium. Biosynthesis of beta-glucosidase was not induced by va...

journal_title：FEMS microbiology letters

authors： Lee J,Kwon KS,Hah YC

更新日期：1996-01-01 00:00:00

abstract：:A group of bacterial strains formerly known as CDC group M-5 are opportunistic pathogens to humans. In 1993, a name, Neisseria weaveri, was proposed by two independent studies to harbor CDC group M-5 strains, namely N. weaveri Holmes et al. 1993 and N. weaveri Andersen et al. 1993, with two different 'type' strains. H...

journal_title：FEMS microbiology letters

authors： Yi H,Cho YJ,Yoon SH,Park SC,Chun J

更新日期：2012-03-01 00:00:00

abstract：:Isolates of anaerobic fungi obtained from the rumen, duodenum and faeces of sheep were identified as Piromyces mae based on their morphological characteristics observed using light microscopy. There was no significant morphological variation among the isolates of P. mae from the rumen, duodenum and faeces. Isozymes of...

journal_title：FEMS microbiology letters

authors： Wong MV,Ho YW,Tan SG,Abdullah N,Jalaludin S

更新日期：1995-12-01 00:00:00

abstract：:Eight-day-old embryonated hen's eggs were used as a model to study Mycobacterium avium virulence. Strains isolated from human patients caused 20-90% mortality when eggs were infected by injection of bacterial suspensions into the amniotic sac. Virulence of examined strains subsequently decreased with passage through e...

journal_title：FEMS microbiology letters

authors： Long EG,Ewing EP Jr,Bartlett JH,Horsburgh CR Jr,Birkness KA,Yakrus MA,Newman GW,Quinn FD

更新日期：2000-09-15 00:00:00

abstract：:In several bacterial species that show natural transformation, dprA has been described as a competence gene. The DprA protein has been suggested to be involved in the protection of incoming DNA. However, members of the dprA gene family (also called smf) can be detected in virtually all bacterial species, which suggest...

journal_title：FEMS microbiology letters

authors： Smeets LC,Becker SC,Barcak GJ,Vandenbroucke-Grauls CM,Bitter W,Goosen N

更新日期：2006-10-01 00:00:00

abstract：:Rifampicin is a principal drug used to combat infections by mycobacteria and related organisms. Most strains of Mycobacterium are able to inactivate this antibiotic by ribosylation via an ADP-ribosylated intermediate. We found that this inactivation was inhibited by light at levels similar to those prevailing in labor...

journal_title：FEMS microbiology letters

authors： Dabbs ER,Quan S

更新日期：2000-01-01 00:00:00

abstract：:We have constructed a very efficient synthesis and secretion system for cholera toxin B subunit (CTB) of Vibrio cholerae 569B using Bacillus-brevis. The constructed expression-secretion vector has the multiple promoters and the signal peptide coding region of the mwp gene, a structural gene for one of the major cell w...

journal_title：FEMS microbiology letters

authors： Ichikawa Y,Yamagata H,Tochikubo K,Udaka S

更新日期：1993-08-01 00:00:00

abstract：:We characterized Yersinia enterocolitica and Yersinia pseudotuberculosis insertion sequences related to insertion sequence 1541, recently identified in Yersinia pestis. For each of the two species, two insertion sequence copies were cloned and sequenced. Genetic elements from Y. pseudotuberculosis were almost identica...

journal_title：FEMS microbiology letters

authors： Devalckenaere A,Odaert M,Trieu-Cuot P,Simonet M

更新日期：1999-07-01 00:00:00

abstract：:Helicobacter pylori ATCC43504 responds chemotactically to aspartic acid and serine, but not to arginine, nor to sodium bicarbonate. In contrast, H. pylori ATCC700392 (strain 26695) shows chemotaxis to all four attractants. Open reading frame HP0099 from H. pylori 26695 is predicted to encode one of three methyl-accept...

journal_title：FEMS microbiology letters

authors： Cerda O,Rivas A,Toledo H

更新日期：2003-07-29 00:00:00

abstract：:CRISPR-Cas systems provide adaptive immunity against mobile genetic elements, but employment of this resistance mechanism is often reported with a fitness cost for the host. Whether or not CRISPR-Cas systems are important barriers for the horizontal spread of conjugative plasmids, which play a crucial role in the spre...

journal_title：FEMS microbiology letters

authors： van Sluijs L,van Houte S,van der Oost J,Brouns SJ,Buckling A,Westra ER

更新日期：2019-03-01 00:00:00

abstract：:In the present study, the homology relationships among fragments amplified by ERIC (Enterobacterial Repetitive Intergenic Consensus) and REP (Repetitive Extragenic Palindromic) primers in Drechslera avenae and Stemphylium solani causing leaf blotch and black spot of oats and leaf blight of cotton, respectively, were i...

journal_title：FEMS microbiology letters

authors： Mehta A,Mehta YR,Rosato YB

更新日期：2002-05-21 00:00:00

abstract：:In the yearly Internationally Genetically Engineered Machines (iGEM) competition, teams of Bachelor's and Master's students design and build an engineered biological system using DNA technologies. Advising an iGEM team poses unique challenges due to the inherent difficulties of mounting and completing a new biological...

journal_title：FEMS microbiology letters

authors： Tsui J,Meyer AS

更新日期：2016-07-01 00:00:00

abstract：:The genus Phytomonas is responsible for many diseases in different crop plant species. The finding that chitin is an exposed cell surface polysaccharide in Phytomonas françai and the observation that chitinases can inhibit fungal growth raises expectations about the potential effect of plant chitinases on the P. franç...

journal_title：FEMS microbiology letters

authors： Gomes Rocha GC,Nicolich R,Romeiro A,Margis-Pinheiro M,Attias M,Alves-Ferreira M

更新日期：2003-09-12 00:00:00

abstract：:Autophagy, a eukaryotic cellular activity leading to the degradation of cellular components, serves as a defense mechanism against facultative intracellular bacteria as well as a growth niche for the obligate intracellular bacterium Coxiella burnetii. We here demonstrate that the obligate intracellular bacterial patho...

journal_title：FEMS microbiology letters

authors： Pachikara N,Zhang H,Pan Z,Jin S,Fan H

更新日期：2009-03-01 00:00:00

abstract：:Salmonella enterica serovar Agona is an important zoonotic pathogen, causing serious human illness worldwide, but knowledge about its genetics and evolution, especially regarding the genomic events that might have contributed to the formation of S. Agona as an important pathogen, is lacking. As a first step toward und...

journal_title：FEMS microbiology letters

authors： Chen F,Poppe C,Liu GR,Li YG,Peng YH,Sanderson KE,Johnston RN,Liu SL

更新日期：2009-04-01 00:00:00

abstract：:Loop-mediated isothermal amplification (LAMP) assay detected Salmonella within 60 min. The 220 strains of 39 serotypes of Salmonella subsp. enterica and 7 strains of Salmonella enterica subsp. arizonae were amplified, but not 62 strains of 23 bacterial species other than Salmonella. The sensitivity of the LAMP assay w...

journal_title：FEMS microbiology letters

authors： Hara-Kudo Y,Yoshino M,Kojima T,Ikedo M

更新日期：2005-12-01 00:00:00

abstract：:In this study, 52 Streptococcus suis isolates from pigs in southern China were divided into four known sequence types (STs) and six new STs, using multilocus sequence typing. Ten representative isolates were selected from 10 STs for the analysis of whole genome sequences. Virulence was assessed in 10 isolates, which w...

journal_title：FEMS microbiology letters

authors： Peng L,Lin M,Huang Z,Guo S,Sun H,Yang X

更新日期：2020-03-01 00:00:00

abstract：:Escherichia coli heat-labile enterotoxin B subunit (EtxB) has been proposed as a potential protein carrier for the delivery of heterologous peptides to target cells, particularly for the oral delivery of epitopes to the mucosal immune system. In this study, two extensions to the C-terminus of EtxB were genetically eng...

journal_title：FEMS microbiology letters

authors： Marcello A,Loregian A,Palù G,Hirst TR

更新日期：1994-03-15 00:00:00

abstract：:The main determinant of the plant pathogen Erwinia chrysanthemi virulence is the production of extracellular enzymes, mainly pectate lyases. Adjacent to a pectate lyase encoding locus, we identified the gene rotA supposed to encode a folding catalyst. Overproduction of the protein and assay of activity using a synthet...

journal_title：FEMS microbiology letters

authors： Pissavin C,Hugouvieux-Cotte-Pattat N

更新日期：1997-12-01 00:00:00

abstract：:The American Society for Microbiology's curricular guidelines for Introductory Microbiology highlighted key laboratory skills in the isolation, visualization and identification of microorganisms as core learning objectives in the discipline. Since the publication of these guidelines in 2012, there has been a paucity o...

journal_title：FEMS microbiology letters

authors： Wang JTH,Huston WM,Johanesen P,Lloyd M,Waller KL

更新日期：2018-10-01 00:00:00

abstract：:The enteric conjugative transposon-like IncJ elements R391, R392, R705, R706 and pMERPH, all demonstrated increased conjugative transfer upon UV irradiation. The transfer frequency increased on average from its basal rate of 10(-5) to 10(-3) per recipient, upon pre-exposure to UV irradiation. However, the transfer fre...

journal_title：FEMS microbiology letters

authors： McGrath BM,O'Halloran JA,Pembroke JT

更新日期：2005-02-15 00:00:00

abstract：:High resistance to class IIa bacteriocins in Listeria monocytogenes has been clearly linked to lack of expression of the mptACD operon, encoding the EIIt Man mannose PTS permease. Also, intermediate resistance has been associated with membrane phospholipid modifications in the spontaneous mutants L. monocytogenes B73-...

journal_title：FEMS microbiology letters

authors： Arous S,Dalet K,Héchard Y

更新日期：2004-09-01 00:00:00

abstract：:Dissimilatory metal-reducing bacteria (DMRB), such as Shewanella oneidensis MR-1, are of great interest for their importance in the biogeochemical cycling of metals and utility in biotechnological processes, such as bioremediation and microbial fuel cells. To identify genes necessary for metal reduction, this study co...

journal_title：FEMS microbiology letters

authors： Kouzuma A,Hashimoto K,Watanabe K

更新日期：2012-01-01 00:00:00

abstract：:A 2918 bp sequence coding for the orotidine-5'-monophosphate decarboxylase enzyme (OMPD) was isolated from the genome of Myrothecium gramineum. This sequence was analysed and, remarkably, it is the first OMPD gene of a Sordariomycete that has an intron. The gene codes for an enzyme of 282 amino acids. The nucleotide s...

journal_title：FEMS microbiology letters

authors： De Maeseneire SL,De Groeve MR,Dauvrin T,De Mey M,Soetaert W,Vandamme EJ

更新日期：2006-08-01 00:00:00

abstract：:In the Pseudomonas fluorescens strain ST the ability to utilize styrene as the sole carbon source is due to the presence of a plasmid, pEG. In the present report we show that pEG contains two inverted repeat sequences and we present evidence indicating that the catabolic genes are localized in these repeats. The regio...

journal_title：FEMS microbiology letters

authors： Ruzzi M,Zennaro E

更新日期：1989-06-01 00:00:00

abstract：:During bacterial infection, professional phagocytes are attracted to the site of infection, where they constitute a first line of host cell defense. Their function is to engulf and destroy the pathogens. Thus, bacteria must withstand the bactericidal activity of professional phagocytes, including macrophages to counte...

journal_title：FEMS microbiology letters

authors： Tran SL,Ramarao N

更新日期：2013-10-01 00:00:00

abstract：:The Gram-positive bacterium Streptococcus gordonii has been genetically engineered to allow the simultaneous expression of two heterologous proteins at the cell surface. A family of recombinant streptococci displaying two different antigens was constructed. All the strains were genetically stable and expressed both pr...

journal_title：FEMS microbiology letters

authors： Maggi T,Spinosa M,Ricci S,Medaglini D,Pozzi G,Oggioni MR

更新日期：2002-04-23 00:00:00