Insertion site of the locus of enterocyte effacement in enteropathogenic and enterohemorrhagic Escherichia coli differs in relation to the clonal phylogeny of the strains.

abstract：:The gene encoding the second enzyme of the 2C-methyl-D-erythritol 4-phosphate (MEP) pathway for isopentenyl diphosphate biosynthesis, 1-deoxy-D-xylulose 5-phosphate (DXP) reductoisomerase, was cloned and sequenced from Zymomonas mobilis. The deduced amino acid sequence showed the highest identity (48.2%) to the DXP re...

journal_title：FEMS microbiology letters

authors： Grolle S,Bringer-Meyer S,Sahm H

更新日期：2000-10-01 00:00:00

abstract：:The production of candidate affinity proteins in a soluble form, for downstream characterization, is often a time-consuming step in combinatorial protein engineering methods. Here, a novel approach for efficient production of candidate clones is described based on direct cleavage of the affinity protein from the surfa...

journal_title：FEMS microbiology letters

authors： Kronqvist N,Löfblom J,Severa D,Ståhl S,Wernérus H

更新日期：2008-01-01 00:00:00

abstract：:Six strains isolated from fermented food were identified as Weissella species by 16S rDNA sequencing, clustering with the species pair W. confusa/W. cibaria. The strains were analysed for growth on glucose, xylose and xylooligosaccharides (XOS). All strains were xylose positive using the API CHL 50 test. Growth on XOS...

journal_title：FEMS microbiology letters

authors： Patel A,Falck P,Shah N,Immerzeel P,Adlercreutz P,Stålbrand H,Prajapati JB,Holst O,Nordberg Karlsson E

更新日期：2013-09-01 00:00:00

abstract：:Nocardia asteroides complex organisms derived from human specimens between 1979 and 1992 were identified to the species level. Of 117 N. asteroides complex organisms, 34 (29%) were N. farcinica, 28 (24%) were N. nova, and 55 (47%) were N. asteroides sensu stricto. An analysis of the specimen sites from which the organ...

journal_title：FEMS microbiology letters

authors： Desmond EP,Flores M

更新日期：1993-07-01 00:00:00

abstract：:In order to find reasons for the absence of fire blight in most countries of the Southern hemisphere, bark samples from apple and pear trees in orchards of the Western Cape region in South Africa were extracted for bacteria which could be antagonistic to Erwinia amylovora. Screening was done in the late growth season ...

journal_title：FEMS microbiology letters

authors： Jock S,Völksch B,Mansvelt L,Geider K

更新日期：2002-06-04 00:00:00

abstract：:In this report we propose a model of apical growth for streptomycetes. The apical tip is considered as a multilayered wall that expands by an inside-to-outside mechanism of growth. It is also assumed that each layer is made up of peptidoglycan blocks, each of them being the result of the biosynthetic activity of a wal...

journal_title：FEMS microbiology letters

authors： Miguélez EM,Martín C,Manzanal MB,Hardisson C

更新日期：1992-12-15 00:00:00

abstract：:The effectiveness of an educational board game developed to teach the pharmacology of antimicrobial drugs to medical students was compared with the lecture-based seminar as a supplemental tool to improve short- and long-term knowledge retention and the perception of the learning method by students. A group of 124 stud...

journal_title：FEMS microbiology letters

authors： Karbownik MS,Wiktorowska-Owczarek A,Kowalczyk E,Kwarta P,Mokros Ł,Pietras T

更新日期：2016-04-01 00:00:00

abstract：:We transformed a clinical Staphylococcus epidermidis isolate with the Enterococcus faecalis transposon Tn917-carrying plasmid pTV1. Loss of plasmid replication was observed at 47 degrees C. Tn917 transposes efficiently and apparently randomly. The transposition frequency could be stimulated by erythromycin. Transposon...

journal_title：FEMS microbiology letters

authors： Grueter L,Koenig O,Laufs R

更新日期：1991-08-01 00:00:00

abstract：:Both ThyA and ThyX proteins catalyze the transfer of the methyl group from methylenetetrahydrofolate (CH(2) H(4) -folate) to dUMP, forming dTMP. To estimate the relative steady state expression levels of ThyA and ThyX, Western blot analysis was performed using ThyA or ThyX antiserum on total protein from the wild-type...

journal_title：FEMS microbiology letters

authors： Cho S,Yang S,Rhie H

更新日期：2012-03-01 00:00:00

abstract：:The gene encoding Aquifex aeolicus (Aae) DNA polymerase was expressed under the control of the trp promoter on a high-copy plasmid, pTRPNS, in Escherichia coli. The expressed enzyme was purified 11-fold with a 13.8% yield and a specific activity of 2268.3 U mg(-1). The optimum pH of the enzyme was 6.8-7.2. The optimal...

journal_title：FEMS microbiology letters

authors： Chang JR,Choi JJ,Kim HK,Kwon ST

更新日期：2001-07-10 00:00:00

abstract：:The symbiotic plasmid (pSym) DNA present in bacteroids of strain RCR1001 of Rhizobium leguminosarum biovar viceae has been compared qualitatively and quantitatively with that present in free living bacteria by hybridization experiments with appropriate probes. A decrease in the relative amount of pSym DNA was observed...

journal_title：FEMS microbiology letters

authors： Vierny C,Iaccarino M

更新日期：1989-07-01 00:00:00

abstract：:In this study, the role of Listeria monocytogenes ferritin was investigated. The fri gene encoding the ferritin was deleted and the phenotype of the mutant was analyzed demonstrating that ferritin is necessary for optimal growth in minimal medium in both presence and absence of iron, as well as after cold- and heat-sh...

journal_title：FEMS microbiology letters

authors： Dussurget O,Dumas E,Archambaud C,Chafsey I,Chambon C,Hébraud M,Cossart P

更新日期：2005-09-15 00:00:00

abstract：:A bacterial isolate, tentatively identified as Pseudomonas sp. strain TR3, was found to utilize the diaryl ester phenylbenzoate as sole source of carbon and energy. This strain has the ability to productively degrade phenylbenzoate and some substituted derivatives by a catabolic sequence which was characterized bioche...

journal_title：FEMS microbiology letters

authors： Reich T,Schmidt S,Fortnagel P

更新日期：1999-07-15 00:00:00

abstract：:Mycoplasma mycoides subsp. mycoides (Mmm) strain Afadé had previously been shown to undergo spontaneous phase variations between an opaque capsulated variant and a translucent (TR) variant devoid of a capsule but able to secrete cell-free exopolysaccharides. This phase variation is associated with an ON/OFF genetic sw...

journal_title：FEMS microbiology letters

authors： Gaurivaud P,Lakhdar L,Le Grand D,Poumarat F,Tardy F

更新日期：2014-10-01 00:00:00

abstract：:Yeast Cia2p is a component of the cytosolic Fe/S protein assembly (CIA) machinery. Initial studies of the CIA machinery were performed in yeast, but the precise role of Cia2p in this eukaryote is still unknown. We report that CIA2 deficiency results in impaired oxidative stress response, as evidenced by increased sens...

journal_title：FEMS microbiology letters

authors： Zhao W,Zheng HZ,Niu YJ,Yuan Y,Fang BX,Liu YN,Cai LH,Zhou ZJ,Liu XG

更新日期：2015-03-01 00:00:00

abstract：:An exo-polygalacturonase (EC 3.2.1.15) was purified to apparent homogeneity from cultures of Fusarium oxysporum f.sp. lycopersici on synthetic medium supplemented with citrus pectin, using preparative isoelectric focusing. The enzyme, denominated PG2, had an apparent M(r) of 74000 Da upon SDS-PAGE. The pI of the main ...

journal_title：FEMS microbiology letters

authors： Di Pietro A,Roncero MI

更新日期：1996-12-01 00:00:00

abstract：:Piscirickettsia salmonis is the etiological agent of Salmonid Rickettsial Septicemia, a disease affecting salmon aquaculture industry. We analyzed the 16S-23S rDNA spacer region (internal transcribed spacer, ITS) of Chilean P. salmonis isolates LF-89 and EM-90. Two main ITS amplification products were obtained by PCR ...

journal_title：FEMS microbiology letters

authors： Casanova A,Obreque J,Sandino AM,Jashés M

更新日期：2001-04-01 00:00:00

abstract：:Tn4560 (8.6 kb) was derived from Tn4556, a Tn3-like element from Streptomyces fradiae. It contains a viomycin resistance gene that has not been used previously for selection in mycobacteria. Tn4560, cloned in a Streptomyces plasmid, was introduced by electroporation into Mycobacterium smegmatis mc(2)155. Tn4560 transp...

journal_title：FEMS microbiology letters

authors： Bhatt A,Stewart GR,Kieser T

更新日期：2002-01-10 00:00:00

abstract：:Hyphae of the human pathogenic fungus Candida albicans exhibit thigmotropic behaviour in vitro, in common with phytopathogenic and saprotrophic fungi. An examination of the literature on C. albicans hyphal penetration of epithelial and endothelial membranes does not support the premise that hyphal thigmotropism plays ...

journal_title：FEMS microbiology letters

authors： Davies JM,Stacey AJ,Gilligan CA

更新日期：1999-02-15 00:00:00

abstract：:High voltage electroporation has been investigated as a method for rapid recovery of plasmid and chromosomal DNA from the cyanobacteria Nostoc PCC 7121, Synechococcus PCC 7002, and Anabaena PCC 7120. Pulses of 18 kV/cm and higher applied to concentrated Nostoc cells carrying a shuttle plasmid (pRL25) resulted in copio...

journal_title：FEMS microbiology letters

authors： Moser D,Zarka D,Hedman C,Kallas T

更新日期：1995-05-15 00:00:00

abstract：:Two Pseudomonas aeruginosa genes encoding the enzymes 1-deoxy-D-xylulose 5-phosphate (DXP) synthase and DXP reductoisomerase, both involved in the mevalonate-independent biosynthesis of isoprenoids, have been expressed as recombinant enzymes in Escherichia coli. The purified P. aeruginosa DXP reductoisomerase was inhi...

journal_title：FEMS microbiology letters

authors： Altincicek B,Hintz M,Sanderbrand S,Wiesner J,Beck E,Jomaa H

更新日期：2000-09-15 00:00:00

abstract：:One of the commercial strains of Flammulina velutipes was analyzed for the presence of double-stranded RNA (dsRNA) elements to examine the underlying mechanism of strain degeneration. As a result, two dsRNA elements sized 1.9 and 1.8 kb were detected in mycelium derived from spontaneously brown-colored fruit body. The...

journal_title：FEMS microbiology letters

authors： Magae Y,Hayashi N

更新日期：1999-11-15 00:00:00

abstract：:Pseudomonas spp., in particular Pseudomonas fragi, are dominant in aerobically stored chilled meats. This work isolated P. fragi isolates from spoiled chicken and investigated the effect of modified atmosphere packaging (MAP, CO2/N2 30%/70%) on physicochemical properties of P. fragi isolates compared to the correspond...

journal_title：FEMS microbiology letters

authors： Wang GY,Li M,Ma F,Wang HH,Xu XL,Zhou GH

更新日期：2017-06-15 00:00:00

abstract：:Biology education is undergoing a transformation toward a more student-centered, inquiry-driven classroom. Many educators have designed engaging assignments that are designed to help undergraduate students gain exposure to the scientific process and data analysis. One of these types of assignments is use of a grant pr...

journal_title：FEMS microbiology letters

authors： Sparks-Thissen RL

更新日期：2017-02-01 00:00:00

abstract：:Among the etiological agents of Lyme disease, Borrelia burgdorferi sensu stricto strains carry a 16 kb plasmid, which did not hybridize to plasmids of B. garinii and B. afzelii strains. A 1271 bp DNA fragment of the 16 kb plasmid was cloned. It hybridized to several plasmids of this species (16, 27 and 55 kb). Sequenc...

journal_title：FEMS microbiology letters

authors： Misonne MC,Schuttler M,Dernelle JM,De Kesel M,Hoet PP

更新日期：1997-05-01 00:00:00

abstract：:A purification scheme was devised for a 69-kDa outer membrane protein of Bordetella pertussis, a virulence-associated protein which may play a role in the pathogenesis of the organism. The protein was purified to apparent homogeneity by heating B. pertussis cells for 1 h at 60 degrees C followed by DEAE-Sepharose and ...

journal_title：FEMS microbiology letters

authors： Gould-Kostka JL,Burns DL,Brennan MJ,Manclark CR

更新日期：1990-02-01 00:00:00

abstract：:While there is an extensive database of genes encoding ammonia monooxygenase (amo) found in the ammonia-oxidizing beta-proteobacteria, few amo sequences are available representing the gamma-proteobacteria. We sequenced the complete amo operon (amoCAB) for Nitrosococcus oceani (ATCC 19707), a marine, autotrophic, ammon...

journal_title：FEMS microbiology letters

authors： Alzerreca JJ,Norton JM,Klotz MG

更新日期：1999-11-01 00:00:00

abstract：:A cDNA from Penicillium minioluteum HI-4 encoding a dextranase (1,6-alpha-glucan hydrolase, EC 3.2.1.11) was isolated and characterized. cDNA clones corresponding to genes expressed in dextran-induced cultures were identified by differential hybridization. Southern hybridization and restriction mapping analysis of sel...

journal_title：FEMS microbiology letters

authors： Garcia B,Margolles E,Roca H,Mateu D,Raices M,Gonzales ME,Herrera L,Delgado J

更新日期：1996-10-01 00:00:00

abstract：:A cordycepin-resistant mutant strain of Saccharomyces cerevisiae (CD-R2) was found to be deficient in adenosine kinase. This mutant accumulated S-adenosylhomocysteine during growth in the presence of exogenous adenosine and it grew in a pseudohyphal manner in the presence of this nucleotide. ...

journal_title：FEMS microbiology letters

authors： Iwashima A,Ogata M,Nosaka K,Nishimura H,Hasegawa T

更新日期：1995-03-15 00:00:00

abstract：:Fusarium graminearum was grown on four lignocellulosic substrates (corn cobs, wheat bran, hop cell walls, and birchwood) and glucose as the sole carbon source. Proteomic studies performed on the resulting enzymatic cocktails highlighted a great diversity in the number and type of proteins secreted. The cell wall-degra...

journal_title：FEMS microbiology letters

authors： Debeire P,Delalande F,Habrylo O,Jeltsch JM,Van Dorsselaer A,Phalip V

更新日期：2014-06-01 00:00:00