Abstract:
:The distribution of the alcohol dehydrogenase gene (adh) among different Archaea was investigated by Southern blot analysis revealing the potentiality of the adh gene as a specific marker for the genus Sulfolobus. Moreover, the in vivo expression of the adh gene from a new isolate of Sulfolobus solfataricus, G theta, was studied to investigate gene regulation in Archaea. Primer extension analysis allowed the identification of a single initiation site and the TATA box element. Comparison of the G theta adh promoter with the corresponding Ssadh (adh from S. solfataricus) and RC3adh (adh from Sulfolobus RC3) also revealed the presence of two putative regulatory inverted repeats at the 5' of the TATA element. Northern blot analysis and enzymatic assays demonstrated that the transcription and expression of the G theta adh gene is regulated by different carbon and energy sources or by the natural substrate of the ADH enzyme.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Cannio R,Fiorentino G,Rossi M,Bartolucci Sdoi
10.1111/j.1574-6968.1999.tb13352.xsubject
Has Abstractpub_date
1999-01-01 00:00:00pages
31-9issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(98)00520-5journal_volume
170pub_type
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