Quantification of disease progression of several microbial pathogens on Arabidopsis thaliana using real-time fluorescence PCR.

Abstract:

:An accurate monitoring of disease progression is important to evaluate disease susceptibility phenotypes. Over the years, Arabidopsis thaliana has become the model species to serve as a host in plant-pathogen interactions. Despite the efforts to study genetic mechanisms of host defense, little efforts are made for a thorough pathogen assessment, often still depending on symptomology. This manuscript describes the use of real-time polymerase chain reaction (PCR) to assess pathogen growth in the host Arabidopsis for a number of frequently studied pathogens. A wide range of correlations between pathogen biomass and fluorescence is demonstrated, demonstrating the theoretical sensitivity of the technique. It is also demonstrated that host DNA does not interfere with the quantification of pathogen DNA over a wide range. Finally, quantification of pathogen biomass in different plant genotypes with a varying degree of resistance shows the capability of this technique to be used for assessment of pathogen development in disease progression.

journal_name

FEMS Microbiol Lett

authors

Brouwer M,Lievens B,Van Hemelrijck W,Van den Ackerveken G,Cammue BP,Thomma BP

doi

10.1016/S0378-1097(03)00759-6

keywords:

subject

Has Abstract

pub_date

2003-11-21 00:00:00

pages

241-8

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378109703007596

journal_volume

228

pub_type

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