PCR differentiation of seventeen genospecies of Acinetobacter.

abstract：:A gene expression system for both Bacillus subtilis and Escherichia coli was developed. The expression vector, pHASH102, produces any combination of promoter and open reading frame to be expressed based on the T-extended cloning method. Because the pHASH series vectors are designed to shuttle between the genome and a ...

journal_title：FEMS microbiology letters

authors： Ohashi Y,Ohshima H,Tsuge K,Itaya M

更新日期：2003-04-11 00:00:00

abstract：:The streptokinase gene of Streptococcus equisimilis H46 was inactivated by plasmid insertion mutagenesis to study the relationship between elaboration of streptokinase and acquisition of cell-associated plasmin activity after incubation of wild-type and mutant cells in media containing plasminogen or plasmin. The resu...

journal_title：FEMS microbiology letters

authors： Malke H,Mechold U,Gase K,Gerlach D

更新日期：1994-02-01 00:00:00

abstract：:The ADP-ribosylation factor (ARF) family of GTPases are highly conserved from yeast to human and regulate vesicle budding. Sec7 domain containing proteins stimulate the guanine nucleotide exchange on Arf proteins, while ARF-GTPase activating proteins stimulate the hydrolysis of GTP. Since vesicle trafficking is import...

journal_title：FEMS microbiology letters

authors： Oscarsson T,Walther A,Lengeler KB,Wendland J

更新日期：2017-12-29 00:00:00

abstract：:The nucleotide sequence of the gene specifying the ethidium efflux system of Escherichia coli has been determined. The translated open reading frame has identified a membrane-bound polypeptide of 110 amino acids (11,960 Da) which shares 42% identity with a staphylococcal protein specifying resistance to ethidium. ...

journal_title：FEMS microbiology letters

authors： Purewal AS

更新日期：1991-08-01 00:00:00

abstract：:The beta-lactamase genes blaA and blaB were detected by PCR amplification in strains of Yersinia enterocolitica biovar 1A isolated from India, Germany, France and the USA. Both genes were detected in all strains. Polymerase chain reaction-restriction fragment length polymorphism revealed genetic heterogeneity in blaA ...

journal_title：FEMS microbiology letters

authors： Sharma S,Mittal S,Mallik S,Virdi JS

更新日期：2006-04-01 00:00:00

abstract：:Piscirickettsia salmonis is the etiological agent of Salmonid Rickettsial Septicemia, a disease affecting salmon aquaculture industry. We analyzed the 16S-23S rDNA spacer region (internal transcribed spacer, ITS) of Chilean P. salmonis isolates LF-89 and EM-90. Two main ITS amplification products were obtained by PCR ...

journal_title：FEMS microbiology letters

authors： Casanova A,Obreque J,Sandino AM,Jashés M

更新日期：2001-04-01 00:00:00

abstract：:NadR is the repressor protein that controls the expression of genes for NAD synthesis. It is also believed to be involved in nucleotide transport. Point mutations conferring different phenotypes were localized to six different regions within the nadR gene. That mutations affecting repression and transport all mapped w...

journal_title：FEMS microbiology letters

authors： Foster JW,Penfound T

更新日期：1993-09-01 00:00:00

abstract：:Two isolates, belonging to a new species of a novel genus of the Phylum "Deinococcus/Thermus ", were recovered from hot spring runoffs on the Island of São Miguel in the Azores. Strains RQ-24(T) and TU-8 are the first cultured representatives of a distinct phylogenetic lineage within this phylum. These strains form or...

journal_title：FEMS microbiology letters

authors： Albuquerque L,Simões C,Nobre MF,Pino NM,Battista JR,Silva MT,Rainey FA,da Costa MS

更新日期：2005-06-15 00:00:00

abstract：:While there is an extensive database of genes encoding ammonia monooxygenase (amo) found in the ammonia-oxidizing beta-proteobacteria, few amo sequences are available representing the gamma-proteobacteria. We sequenced the complete amo operon (amoCAB) for Nitrosococcus oceani (ATCC 19707), a marine, autotrophic, ammon...

journal_title：FEMS microbiology letters

authors： Alzerreca JJ,Norton JM,Klotz MG

更新日期：1999-11-01 00:00:00

abstract：:Tolaasin is a peptide toxin produced by Pseudomonas tolaasii and causes brown blotch disease of the cultivated mushrooms. Two types of ion channels were identified by the incorporation of tolaasin into lipid bilayer. The slope conductance of type 1 channel measured in the buffer containing 100 mM KCl was 150 pS with a...

journal_title：FEMS microbiology letters

authors： Cho KH,Kim YK

更新日期：2003-04-25 00:00:00

abstract：:Trichothecenes are an important family of mycotoxins produced by several species of the genus Fusarium. These fungi cause serious disease on infected plants and postharvest storage of crops, and the toxins can cause health problems for humans and animals. Unfortunately, there are few methods for controlling mycotoxin ...

journal_title：FEMS microbiology letters

authors： Ismail Y,McCormick S,Hijri M

更新日期：2013-11-01 00:00:00

abstract：:We have constructed a DNA library from a virulent Salmonella typhimurium strain, in an avirulent strain. The process of selecting the components of interest from the library involved iterative growth in liquid culture. This resulted, after four cycles, in the culture becoming homogeneous for a single plasmid, which wa...

journal_title：FEMS microbiology letters

authors： Lodge JM,Robey M,Stephen J,Brown NL

更新日期：1999-01-15 00:00:00

abstract：:Ammonia-induced injury was investigated in pure cultures of Escherichia coli and Enterobacter aerogenes, and in natural coliform populations obtained from the oligotrophic Luxapallila and the eutrophic Sunflower Rivers in northern Mississippi. Pure cultures were affected by ammonia exposure as indicated by changes in ...

journal_title：FEMS microbiology letters

authors： Naundorf G,Aumen NG

更新日期：1989-11-01 00:00:00

abstract：:Bacterial populations produce persister cells that neither grow nor die in the presence of microbicidal antibiotics. Persisters are largely responsible for high levels of biofilm tolerance to antimicrobials, but virtually nothing was known about their biology. Tolerance of Escherichia coli to ampicillin and ofloxacin ...

journal_title：FEMS microbiology letters

authors： Keren I,Kaldalu N,Spoering A,Wang Y,Lewis K

更新日期：2004-01-15 00:00:00

abstract：:The Mfa1 protein of Porphyromonas gingivalis is the structural subunit of the short fimbriae and mediates coadhesion between P. gingivalis and Streptococcus gordonii. We utilized a promoter-lacZ reporter construct to examine the regulation of mfa1 expression in consortia with common oral plaque bacteria. Promoter acti...

journal_title：FEMS microbiology letters

authors： Park Y,James CE,Yoshimura F,Lamont RJ

更新日期：2006-09-01 00:00:00

abstract：:IS1(SB) and its two variants were identified as the major and minor IS1 elements in Shigella boydii. The nucleotide sequences of IS1(SB), IS1(O157:H7) from Escherichia coli O157:H7 and IS1F from E. coli K12 suggest that these IS1 elements had been horizontally transferred among S. boydii and E. coli O157:H7 and K12. T...

journal_title：FEMS microbiology letters

authors： Hsu WB,Chen JH

更新日期：2003-05-28 00:00:00

abstract：:The Mycobacterium marinum G13 promoter is a sigma 70-like promoter that is more active by green fluorescent protein (gfp) differential fluorescence induction (DFI) assays when M. marium resides in an intracellular compartment as compared with growth in media. In assays using DFI, we found that the mycobacterial G13 pr...

journal_title：FEMS microbiology letters

authors： Gall K,Barker LP

更新日期：2006-02-01 00:00:00

abstract：:We isolated and characterized nodA genes from photosynthetic and non-photosynthetic rhizobia nodulating the legume genus Aeschynomene, and found that the nodA sequence from photosynthetic stem-nodulating bacteria was phylogenetically distant from the other already described nodA genes. Characterization of the photosyn...

journal_title：FEMS microbiology letters

authors： Chaintreuil C,Boivin C,Dreyfus B,Giraud E

更新日期：2001-01-01 00:00:00

abstract：:We present draft genome sequences of three Holospora species, hosted by the ciliate Paramecium caudatum; that is, the macronucleus-specific H. obtusa and the micronucleus-specific H. undulata and H. elegans. We investigate functions of orthologous core genes conserved across the three Holospora species, which may be e...

journal_title：FEMS microbiology letters

authors： Dohra H,Tanaka K,Suzuki T,Fujishima M,Suzuki H

更新日期：2014-10-01 00:00:00

abstract：:The locus of enterocyte effacement pathogenicity island confers the attaching and effacing histopathology on epithelial cells infected with enteropathogenic and enterohemorrhagic Escherichia coli. We investigated the site of insertion of the locus of enterocyte effacement in E. coli strains in relation to their evolut...

journal_title：FEMS microbiology letters

authors： Wieler LH,McDaniel TK,Whittam TS,Kaper JB

更新日期：1997-11-01 00:00:00

abstract：:The expression of nifA-, niH- and nifB-lacZ fusions was examined in different mutants of Azospirillum brasilense. Mutations in nifA, glnA and glnB severely impaired the expression of nifH- and nifB-lacZ fusions. By contrast, a nifA-lacZ fusion was not affected in a nifA or a glnB background and was only partially impa...

journal_title：FEMS microbiology letters

authors： Liang YY,de Zamaroczy M,Arsène F,Paquelin A,Elmerich C

更新日期：1992-12-15 00:00:00

abstract：:Two interlinked quorum sensing circuits, las and rhl, which control pathogenesis of Pseudomonas aeruginosa are further modulated by numerous regulators, including VqsR (virulence and quorum sensing regulator). High-density oligonucleotide microarrays were used to compare the global expression profile of a wild-type an...

journal_title：FEMS microbiology letters

authors： Juhas M,Wiehlmann L,Salunkhe P,Lauber J,Buer J,Tümmler B

更新日期：2005-01-15 00:00:00

abstract：:The sepsis caused by Vibrio vulnificus is characterized by an average incubation period of 26 h and a high mortality rate exceeding 50%. The fast growth and dissemination of V. vulnificus in vivo lead to poor clinical outcomes in patients. Therefore, elucidation of the proliferation mechanisms of this organism in vivo...

journal_title：FEMS microbiology letters

authors： Kado T,Kashimoto T,Yamazaki K,Ueno S

更新日期：2017-01-01 00:00:00

abstract：:A physical map of the Enterococcus faecium ATCC19434 chromosome was constructed by NotI, I-CeuI and Sse8387I. The chromosome was a circular DNA of 2600 kb in size, and contained six rRNA operons (rrn). The locations and orientations of the six rrn operons and 24 different determinants were mapped. Genomes of three add...

journal_title：FEMS microbiology letters

authors： Oana K,Okimura Y,Kawakami Y,Hayashida N,Shimosaka M,Okazaki M,Hayashi T,Ohnishi M

更新日期：2002-02-05 00:00:00

abstract：:The effect of Listeria monocytogenes infection on the cellular level of transcription factor NF-kappa B in the human epithelia-like cell line Caco-2 was investigated. Infection with L. monocytogenes or treatment with lipoteichoic acid induced the formation of three NF-kappa B-like DNA-protein complexes C1, C2, and C3,...

journal_title：FEMS microbiology letters

authors： Hauf N,Goebel W,Fiedler F,Kuhn M

更新日期：1999-09-01 00:00:00

abstract：:Helicobacter pylori possesses a gene (HP0326/JHP309) homologous to neuA of other bacteria, encoding a cytidyl monophosphate-N-acetylneuraminic acid synthetase-homologous enzyme in its N-terminal portion. We analysed the function of this gene, which is controlled by a flagellar class 2 sigma(54) promoter, in flagellar ...

journal_title：FEMS microbiology letters

authors： Josenhans C,Vossebein L,Friedrich S,Suerbaum S

更新日期：2002-05-07 00:00:00

abstract：:Legionella pneumophila is known as a facultative intracellular parasite of free-living soil and freshwater amoebae, of which several species have been shown to support the growth of the pathogenic bacteria. We report for the first time the behaviour of two strains (c2c and Z503) of the amoeba Willaertia magna towards ...

journal_title：FEMS microbiology letters

authors： Dey R,Bodennec J,Mameri MO,Pernin P

更新日期：2009-01-01 00:00:00

abstract：:As shown by both bioassay and high-performance liquid chromatographic (HPLC) analysis, penicillin G production by Aspergillus nidulans is subject to regulation by the pH of the growth medium. Penicillin titres were highest at alkaline pH and in strains carrying mutations in the regulatory gene pacC which mimics the ef...

journal_title：FEMS microbiology letters

authors： Shah AJ,Tilburn J,Adlard MW,Arst HN Jr

更新日期：1991-01-15 00:00:00

abstract：:A Deltalgt (Lgt, lipoprotein diacylglyceryl transferase) isogenic mutant was obtained which indicates that lgt is not essential for cell growth in vitro, like in the Gram-positive bacterium Bacillus subtilis, but unlike in the proteobacteria Escherichia coli and Salmonella typhimurium. The mutation was transduced to a...

journal_title：FEMS microbiology letters

authors： Petit CM,Brown JR,Ingraham K,Bryant AP,Holmes DJ

更新日期：2001-06-25 00:00:00

abstract：:The anr gene of Pseudomonas aeruginosa encodes a transcriptional regulator of anaerobic gene expression, homologous to the Fnr protein of Escherichia coli. We report here that Anr has a role in regulating the activity of the aerobic respiratory chain of P. aeruginosa. Strains with internal deletions in their anr gene ...

journal_title：FEMS microbiology letters

authors： Ray A,Williams HD

更新日期：1997-11-15 00:00:00