A novel strategy for enzymatic synthesis of 4-hydroxyisoleucine: identification of an enzyme possessing HMKP (4-hydroxy-3-methyl-2-keto-pentanoate) aldolase activity.

abstract：:A fast screening method was developed to assess the pathogenicity of a diverse collection of environmental and clinical Burkholderia cepacia complex isolates in the nematode Caenorhabditis elegans. The method was validated by comparison with the standard slow-killing assay. We observed that the pathogenicity of B. cep...

journal_title：FEMS microbiology letters

authors： Cardona ST,Wopperer J,Eberl L,Valvano MA

更新日期：2005-09-01 00:00:00

abstract：:We report the draft genome sequence from Aeromonas salmonicida sp. strain CBA100, which was characterized as an antibiotic-resistant bacterium isolated from infected rainbow trout. The total size of the genome is 4,788,109 bp, with a G + C content of 60.55%. Comparison of its open reading frames shows that the closest...

journal_title：FEMS microbiology letters

authors： Valdes N,Espinoza C,Sanhueza L,Gonzalez A,Corsini G,Tello M

更新日期：2015-03-01 00:00:00

abstract：:The transport of radioactively labelled uracil into submerged mycelium of T. viride was measured by means of a membrane filtration technique. It was found to be time-dependent (up to 90 min) and concentration-dependent (up to 8 mmol l-1). Its concentration dependence was biphasic and consisted from the saturatable par...

journal_title：FEMS microbiology letters

authors： Lakatos B,Simkovic M,Betina V,Varecka L

更新日期：1999-02-15 00:00:00

abstract：:Among aerobic prokaryotes, many different terminal oxidase complexes have been described. Sequence comparison has revealed that the aa3-type cytochrome c oxidase and the bo3-type quinol oxidase are variations on the same theme: the heme-copper oxidase. A third member of this family has recently been recognized: the cb...

journal_title：FEMS microbiology letters

authors： van der Oost J,de Boer AP,de Gier JW,Zumft WG,Stouthamer AH,van Spanning RJ

更新日期：1994-08-01 00:00:00

abstract：:A Na(+)/H(+) antiporter gene was isolated from alkaliphilic Bacillus sp. G1. The full-length sequence of the Na(+)/H(+) antiporter gene was obtained using a genome walking method, and designated as g1-nhaC. An ORF preceded by a promoter-like sequence and a Shine-Dalgarno sequence, and followed by a terminator-like seq...

journal_title：FEMS microbiology letters

authors： Liew CW,Illias RM,Mahadi NM,Najimudin N

更新日期：2007-11-01 00:00:00

abstract：:DNA hybridization using the Synechocystis PCC6803 glucose transporter gene, glcP, revealed a single homologous region in two facultative photoautotrophic strains out of three tested, and none in three obligate autotrophs. In one of the latter, Synechococcus PCC7942, integration of glcP into the chromosome resulted in ...

journal_title：FEMS microbiology letters

authors： Zhang CC,Jeanjean R,Joset F

更新日期：1998-04-15 00:00:00

abstract：:Lineage-specific genes (LSGs) are defined as genes with sequences that are not significantly similar to those in any other lineage. LSGs have been proposed, and sometimes shown, to have significant effects in the evolution of biological function. In this study, two sets of Hanseniaspora spp. LSGs were identified by co...

journal_title：FEMS microbiology letters

authors： Chen K,Tian Z,Chen P,He H,Jiang F,Long CA

更新日期：2020-06-01 00:00:00

abstract：:This study characterizes the glucomannan utilization operon (gmuBACDREFG, formerly ydhMNOPQRST) of Bacillus subtilis. Transcription of the operon is induced by konjac glucomannan and requires the last mannanase gene (gmuG). Cellobiose and mannobiose, possible degradation products of glucomannan by GmuG, are strong ind...

journal_title：FEMS microbiology letters

authors： Sadaie Y,Nakadate H,Fukui R,Yee LM,Asai K

更新日期：2008-02-01 00:00:00

abstract：:Clostridium difficile and Clostridium perfringens type A are infectious agents of enteric diseases. The main virulence factors of these microorganisms include toxins A and B of C. difficile (ToxA and ToxB) and enterotoxin of C. perfringens (Cpe). In this study genetic constructions have been created for the expression...

journal_title：FEMS microbiology letters

authors： Belyi IF,Varfolomeeva NA

更新日期：2003-08-29 00:00:00

abstract：:In Bacillus subtilis, utilisation of xylose, arabinose and ribose is controlled by the transcriptional factors XylR, AraR and RbsR, respectively. Here we apply the comparative approach to the analysis of these regulons in the Bacillus/Clostridium group. Evolutionary variability of operon structures is demonstrated and...

journal_title：FEMS microbiology letters

authors： Rodionov DA,Mironov AA,Gelfand MS

更新日期：2001-12-18 00:00:00

abstract：:Salicylate was found to uniquely induce a 27-kDa protein in Mycobacterium tuberculosis complex organisms but not in Mycobacterium smegmatis or Escherichia coli. The structural analogue antitubercular para-amino-salicylate also induced the 27-kDa protein but to a somewhat lower level than salicylate. Other structural a...

journal_title：FEMS microbiology letters

authors： Sun Z,Cheng SJ,Zhang H,Zhang Y

更新日期：2001-09-25 00:00:00

abstract：:Brucella abortus B19, an avirulent strain obtained by spontaneous mutation, is used worldwide as a vaccine for the control of bovine brucellosis. B19 differs from other B. abortus strains in its sensitivity to erythritol. We took advantage of a previously obtained erythritol sensitive Tn5 insertion mutant of B. abortu...

journal_title：FEMS microbiology letters

authors： Sangari FJ,García-Lobo JM,Agüero J

更新日期：1994-09-01 00:00:00

abstract：:The genus Xanthomonas is composed of several species that cause severe crop losses around the world. In Latin America, one of the most relevant species is Xanthomonas campestris pv. campestris, which is responsible for black rot in cruciferous plants. This pathogen causes yield losses in several cultures, including ca...

journal_title：FEMS microbiology letters

authors： Andrade AE,Silva LP,Pereira JL,Noronha EF,Reis FB Jr,Bloch C Jr,dos Santos MF,Domont GB,Franco OL,Mehta A

更新日期：2008-04-01 00:00:00

abstract：:Lactic acid bacteria were screened for methional production from 4-methylthio-2-ketobutanoate. Only Lactococcus lactis IFPL730 produced high amounts of methional. It was demonstrated that production of this compound was an exclusively enzymatic reaction. The present work describes for the first time that L. lactis can...

journal_title：FEMS microbiology letters

authors： Amárita F,Fernández-Esplá D,Requena T,Pelaez C

更新日期：2001-10-16 00:00:00

abstract：:The filamentous ascomycete Botrytis cinerea is one of the most studied models for understanding the necrotrophic behaviour of phytopathogenic fungi. The genomes of two strains of B. cinerea have been sequenced (B05.10 and T4), which may contribute to elucidating the virulence polymorphism in this fungus. In this study...

journal_title：FEMS microbiology letters

authors： Choquer M,Robin G,Le Pêcheur P,Giraud C,Levis C,Viaud M

更新日期：2008-12-01 00:00:00

abstract：:The wine yeast Saccharomyces cerevisiae is central in the production of aroma compounds during fermentation. Some of the most important yeast-derived aroma compounds produced are esters. The esters ethyl acetate and isoamyl acetate are formed from alcohols and acetyl-CoA in a reaction catalysed by alcohol acetyltransf...

journal_title：FEMS microbiology letters

authors： Cordente AG,Swiegers JH,Hegardt FG,Pretorius IS

更新日期：2007-02-01 00:00:00

abstract：:The photoinduced bactericidal capacity of TiO(2) based films was evaluated, using as model organism Pseudomonas aeruginosa. Thin films were obtained by spray pyrolysis; they included undoped, Cu doped, and Al doped TiO(2). Scanning electron microscopy was used to observe the final effect of the irradiated films upon t...

journal_title：FEMS microbiology letters

authors： Amézaga-Madrid P,Nevárez-Moorillón GV,Orrantia-Borunda E,Miki-Yoshida M

更新日期：2002-06-04 00:00:00

abstract：:Bordetella calmodulin-like protein was purified from culture supernatant fluid of B. pertussis, B. parapertussis and B. bronchiseptica by successive chromatography on hydroxyapatite, Toyopearl HW-50F and QAE-Toyopearl 550C columns. The purified calmodulin-like protein appeared to be homogeneous by SDS-polyacrylamide g...

journal_title：FEMS microbiology letters

authors： Nagai M,Endoh M,Danbara H,Nakase Y

更新日期：1994-02-15 00:00:00

abstract：:Loop-mediated isothermal amplification (LAMP) assay detected Salmonella within 60 min. The 220 strains of 39 serotypes of Salmonella subsp. enterica and 7 strains of Salmonella enterica subsp. arizonae were amplified, but not 62 strains of 23 bacterial species other than Salmonella. The sensitivity of the LAMP assay w...

journal_title：FEMS microbiology letters

authors： Hara-Kudo Y,Yoshino M,Kojima T,Ikedo M

更新日期：2005-12-01 00:00:00

abstract：:The relationship of RNase P RNA from anammox bacteria 'Candidatus Brocadia anammoxidans' and 'Candidatus Kuenenia stuttgartiensis' with that from other Planctomycetes was investigated. Newly identified rnpB gene sequences were aligned against existing planctomycete RNase P RNA sequences and secondary structures deduce...

journal_title：FEMS microbiology letters

authors： Butler MK,Op den Camp HJ,Harhangi HR,Lafi FF,Strous M,Fuerst JA

更新日期：2007-03-01 00:00:00

abstract：:In this study, we analysed the diversity and composition of double-stranded DNA viral and bacterial communities within the sample of surface coastal water of Southern Baikal through metagenomics and deep sequencing of the 16S ribosomal RNA gene, respectively. The 16S rRNA gene analysis has revealed 14 phyla and domina...

journal_title：FEMS microbiology letters

authors： Butina TV,Bukin YS,Krasnopeev AS,Belykh OI,Tupikin AE,Kabilov MR,Sakirko МV,Belikov SI

更新日期：2019-05-01 00:00:00

abstract：:The molecular genetics of indole-3-acetic (IAA) synthesis and regulation in Azospirillum brasilense was investigated in this study. Tn5 mutagenesis was performed and five mutants with decreased IAA production were isolated. Five Tn5-inserted genes from these mutants were cloned and sequenced. Four genes were reported ...

journal_title：FEMS microbiology letters

authors： Xie B,Xu K,Zhao HX,Chen SF

更新日期：2005-07-01 00:00:00

abstract：:The effect feeding antibiotics has on the bacterial population of the rabbit caecum was investigated. No changes in total volatile fatty acid production or total bacterial counts were observed compared with nonantibiotic treated controls. However, treatment with chlortetracycline resulted in an increase of propionate ...

journal_title：FEMS microbiology letters

authors： Abecia L,Fondevila M,Balcells J,Edwards JE,Newbold CJ,McEwan NR

更新日期：2007-07-01 00:00:00

abstract：:The frequency of spontaneous as well as induced reversions of auxotrophic mutations in Escherichia coli AB1157 and its DeltadnaK and DeltadnaKdnaJ derivatives was estimated. The obtained results demonstrate that both mutants tested are characterized by elevated frequency of spontaneous reversions compared to their AB1...

journal_title：FEMS microbiology letters

authors： Grudniak AM,Kuć M,Wolska KI

更新日期：2005-01-15 00:00:00

abstract：:The outer membrane proteins of several prominent bacterial pathogens demonstrate substantial variation in their surface antigenic epitopes. To determine if this was also true for Pseudomonas aeruginosa outer membrane protein OprF, gene sequencing of a serotype 5 isolate was performed to permit comparison with the publ...

journal_title：FEMS microbiology letters

authors： Martin NL,Rawling EG,Wong RS,Rosok M,Hancock RE

更新日期：1993-11-01 00:00:00

abstract：:The virulence of Salmonella typhimurium for mice is dependent on a plasmid-borne gene cluster termed spv. We previously determined that both S. typhimurium and Escherichia coli bacteria grown in a rich medium preferentially express the spv genes during the stationary phase of growth. In this study we evaluated the rol...

journal_title：FEMS microbiology letters

authors： Norel F,Robbe-Saule V,Popoff MY,Coynault C

更新日期：1992-12-01 00:00:00

abstract：:The lipid modification and processing of truncated mutants of the colicin A lysis protein were observed after overproduction by Escherichia coli bacteria, but at a rate far slower than that of the wild-type. The unmodified precursor form of the mutants was stable over hour(s). The truncated mutants provoked lethality,...

journal_title：FEMS microbiology letters

authors： Cavard D

更新日期：1994-04-01 00:00:00

abstract：:Staphylococcus aureus produces a number of virulence factors. The major virulence factors exhibited by S aureus include various antigens, enzymes, cytotoxins and exotoxins (e.g. hemolysins, enterotoxins and toxic shock syndrome toxin). In this report, we show the influence of punicalagin on the secretion of exoprotein...

journal_title：FEMS microbiology letters

authors： Mun SH,Kong R,Seo YS,Zhou T,Kang OH,Shin DW,Kwon DY

更新日期：2016-11-01 00:00:00

abstract：:The formation of landomycin A or one of its derivatives (5,6-anhydrolandomycin A) in a heterologous strain has never been achieved. It has now been made possible by the coexpression of a cosmid containing all biosynthetic genes necessary to produce landomycin A together with a pathway-specific regulatory gene. As host...

journal_title：FEMS microbiology letters

authors： von Mulert U,Luzhetskyy A,Hofmann C,Mayer A,Bechthold A

更新日期：2004-01-15 00:00:00

abstract：:The c-type cytochrome (OmcB) and the multicopper protein (OmpB) required for Fe(III) oxide reduction by Geobacter sulfurreducens were predicted previously to be outer membrane proteins, but it is not clear whether they are positioned in a manner that permits the interaction with Fe(III). Treatment of whole cells with ...

journal_title：FEMS microbiology letters

authors： Qian X,Reguera G,Mester T,Lovley DR

更新日期：2007-12-01 00:00:00