Lysis of staphylococcal mastitis pathogens by bacteriophage phi11 endolysin.

abstract：:Sera, from 17 patients with diarrhoea or haemolytic uraemic syndrome, and six healthy adults, were tested for neutralisation of Vero cytotoxins (VT). For all 17 patients there was evidence of infection with Escherichia coli O157. Sera from two controls but from none of the patients neutralised VT1, although two patien...

journal_title：FEMS microbiology letters

authors： Scotland SM,Said B,Thomas A,Rowe B

更新日期：1994-01-15 00:00:00

abstract：:Protein A from the Staphylococcus aureus strain V8 has a molecular mass about 8000 Da less than that of known proteins A. The corresponding gene was cloned and expressed in Escherichia coli. Sequence analysis of this structurally new protein A revealed that it lacked an IgG-binding domain (58 amino acids), and that it...

journal_title：FEMS microbiology letters

authors： Finck-Barbançon V,Prevost G,Mazurier I,Piemont Y

更新日期：1992-02-01 00:00:00

abstract：:The flaA gene, which codes for the Spirochaeta aurantia flagellar filament outer layer polypeptide, FlaA, was cloned, sequenced and analysed. The gene appears to be transcribed into a monocistronic mRNA from a sigma 70-like promoter. The translational start is 31 base pairs after the start of the transcript, the open ...

journal_title：FEMS microbiology letters

authors： Parales J Jr,Greenberg EP

更新日期：1993-02-01 00:00:00

abstract：:A synthetic version of the ligA gene encoding the NAD-dependent DNA ligase from the Arctic bacterium Pseudoalteromonas haloplanktis was substituted for its homolog in the chromosome of Mycobacterium smegmatis. The resulting recombinant strain grew identically to the parent strain at permissive temperatures but failed ...

journal_title：FEMS microbiology letters

authors： Pinto CT,Nano FE

更新日期：2015-09-01 00:00:00

abstract：:Ammonium transporters form a conserved family of transport proteins and are widely distributed among all domains of life. The genome of Nitrosomonas europaea codes for a single gene (rh1) that belongs to the family of the AMT/Rh ammonium transporters. For the first time, this study provides functional and physiologica...

journal_title：FEMS microbiology letters

authors： Weidinger K,Neuhäuser B,Gilch S,Ludewig U,Meyer O,Schmidt I

更新日期：2007-08-01 00:00:00

abstract：:The 2.5-kb erythromycin resistance (EmR) plasmid pPV142 of Staphylococcus simulans 13044 was isolated and characterized. Sequence analysis identified ORF1 and ORF2 encoding a 158-residue replication protein (Rep142) and a 244-residue erythromycin resistance protein (Erm, rRNA adenine N-6-methyltransferase), respective...

journal_title：FEMS microbiology letters

authors： Somkuti GA,Solaiman DK,Steinberg DH

更新日期：1998-08-15 00:00:00

abstract：:The microbial adhesion to hydrocarbons (MATH) test is one of the most common method to determine the hydrophobicity of cell surfaces. Despite its prevalence, no standard test parameters are used in literature, making a direct comparison of data almost impossible. Criticism also focuses on test parameters that may mask...

journal_title：FEMS microbiology letters

authors： Nachtigall C,Weber C,Rothenburger S,Jaros D,Rohm H

更新日期：2019-06-01 00:00:00

abstract：:We analyzed gel electrophoretic banding patterns of penicillin-binding proteins (PBPs) of 16 type strains of coagulase-negative staphylococci (CNS). S. epidermidis, S. haemolyticus, S. saprophyticus, S. hominis, S. xylosus, S. simulans, S. warneri, S. capitis, S. saccharolyticus, S. auricularis, S. caseolyticus, S. ga...

journal_title：FEMS microbiology letters

authors： Kanda K,Hiramatsu K,Yokota T

更新日期：1990-10-01 00:00:00

abstract：:In this study, 29 strains of Kluyveromyces marxianus with peculiar genetic and phenotypic traits previously isolated from a fermented goat milk of Yaghnob valley were investigated for chromosome length polymorphism (CLP) by PFGE, adhesion properties and carbon usage by Biolog analysis. Obtained data showed that strain...

journal_title：FEMS microbiology letters

authors： Perpetuini G,Tittarelli F,Mattarelli P,Modesto M,Cilli E,Suzzi G,Tofalo R

更新日期：2018-03-01 00:00:00

abstract：:A high-level expression system for the Escherichia coli HB101 gamma-glutamyltranspeptidase (GGT) gene was constructed to obtain a sufficient amount of the enzyme for structural studies. About 300 mg of the enzyme was purified from 2 1 of culture broth. Both subunits were needed to reconstitute GGT activity. The E. col...

journal_title：FEMS microbiology letters

authors： Ishiye M,Niwa M

更新日期：1992-10-15 00:00:00

abstract：:A bacterial isolate, tentatively identified as Pseudomonas sp. strain TR3, was found to utilize the diaryl ester phenylbenzoate as sole source of carbon and energy. This strain has the ability to productively degrade phenylbenzoate and some substituted derivatives by a catabolic sequence which was characterized bioche...

journal_title：FEMS microbiology letters

authors： Reich T,Schmidt S,Fortnagel P

更新日期：1999-07-15 00:00:00

abstract：:Two Csp proteins (CspA and CspD) were fused to the green fluorescent protein GFP and expressed from their natural promoters or from an inducible promoter. Fluorescence microscopy and computerized image analysis indicate that in Escherichia coli growing at 37 degrees C CspD localizes in the nucleoid like the control H-...

journal_title：FEMS microbiology letters

authors： Giangrossi M,Exley RM,Le Hegarat F,Pon CL

更新日期：2001-08-21 00:00:00

abstract：:A bacterial strain, designated as TSB-6, was isolated from the sediments of a Tantloi (India) hot spring at 65 °C. The strain showed 98% 16S rRNA gene sequence similarity with Anoxybacillus kualawohkensis strain KW12 and was found to grow optimally at 37 °C. However, growing cells, cell suspensions, and cell-free extr...

journal_title：FEMS microbiology letters

authors： Paul SC,Jain P,Mitra J,Dutta S,Bhattacharya P,Bal B,Bhattacharyya D,Das Gupta S,Pal S

更新日期：2012-06-01 00:00:00

abstract：:It was speculated that the increase of the UDP-GlcNAc pool observed with chloramphenicol can modulate the residual PEP:UDP-GlcNAc-enolpyruvate activity of fosfomycin-treated cells. This provided an explanation on how chloramphenicol can insure the formation of enough UDP-MurNAc-pentapeptide to sustain peptidoglycan sy...

journal_title：FEMS microbiology letters

authors： Mengin-Lecreulx D,van Heijenoort J

更新日期：1990-01-01 00:00:00

abstract：:Penicillium expansum is the causal agent of blue mold rot, a postharvest decay of stored fruits. This fungus also produces the mycotoxins patulin and citrinin. Control of P. expansum still relies mainly on the use of fungicides such as thiabendazole. Since its introduction, resistant strains have been reported. The ai...

journal_title：FEMS microbiology letters

authors： Cabañas R,Castellá G,Abarca ML,Bragulat MR,Cabañes FJ

更新日期：2009-08-01 00:00:00

abstract：:An obligately anaerobic spirochete designated strain SEBR 4228T (T = type strain) was isolated from an oil field of Congo, Central Africa. The strain grew optimally with a sodium chloride concentration of 5% (sodium chloride concentration) growth range 1.0-10%) at 37 degrees C (growth temperature range 20-40 degrees C...

journal_title：FEMS microbiology letters

authors： Magot M,Fardeau ML,Arnauld O,Lanau C,Ollivier B,Thomas P,Patel BK

更新日期：1997-10-15 00:00:00

abstract：:In this study, we show that berberine chloride (BBR) has antimicrobial activities against all 43 tested strains of Staphylococcus aureus, an important human and animal pathogen. However, the response mechanisms of S. aureus to BBR are still poorly understood. Affymetrix GeneChips were used to determine the global tran...

journal_title：FEMS microbiology letters

authors： Wang D,Yu L,Xiang H,Fan J,He L,Guo N,Feng H,Deng X

更新日期：2008-02-01 00:00:00

abstract：:The scrA gene coding for sucrose EnzymeII of the phosphoenolpyruvate dependent phosphotransferase system previously isolated from Streptococcus mutans was fused in vitro to the promoterless lacZ' gene to monitor the expression of the scrA gene. The scrA::lacZ gene fusion was introduced back into S. mutans GS-5IS3 by t...

journal_title：FEMS microbiology letters

authors： Sato Y,Yamamoto Y,Suzuki R,Kizaki H,Kuramitsu HK

更新日期：1991-04-15 00:00:00

abstract：:Competition assays measure differences between populations of bacteria after stress adaptation, populations of different bacteria and mutations in antibiotic resistance genes. We have developed a competition-based assay to evaluate if genes upregulated under starvation are important for bacterial survival. Stress resp...

journal_title：FEMS microbiology letters

authors： Havis S,Rangel J,Mali S,Bodunrin A,Housammy Z,Zimmerer R,Murphy J,Widger WR,Bark SJ

更新日期：2019-03-01 00:00:00

abstract：:Trichothecenes are an important family of mycotoxins produced by several species of the genus Fusarium. These fungi cause serious disease on infected plants and postharvest storage of crops, and the toxins can cause health problems for humans and animals. Unfortunately, there are few methods for controlling mycotoxin ...

journal_title：FEMS microbiology letters

authors： Ismail Y,McCormick S,Hijri M

更新日期：2013-11-01 00:00:00

abstract：:Mathematical modeling is an important tool in biological research, allowing for the synthesis of results from many studies into an understanding of a system. Despite this, the need for extensive subject matter knowledge and complex mathematics often leaves modeling as an esoteric subspecialty. A 2-fold approach can be...

journal_title：FEMS microbiology letters

authors： Lofgren ET

更新日期：2016-10-01 00:00:00

abstract：:The occurrence of highly variable penicillin-binding proteins (PBPs) in penicillin-resistant Streptococcus pneumoniae suggested that transfer of homologous genes from related species may be involved in resistance development. Antiserum and monoclonal antibodies raised against PBPs 1a and 2b from the susceptible S. pne...

journal_title：FEMS microbiology letters

authors： Chalkley L,Schuster C,Potgieter E,Hakenbeck R

更新日期：1991-12-15 00:00:00

abstract：:The enteric conjugative transposon-like IncJ elements R391, R392, R705, R706 and pMERPH, all demonstrated increased conjugative transfer upon UV irradiation. The transfer frequency increased on average from its basal rate of 10(-5) to 10(-3) per recipient, upon pre-exposure to UV irradiation. However, the transfer fre...

journal_title：FEMS microbiology letters

authors： McGrath BM,O'Halloran JA,Pembroke JT

更新日期：2005-02-15 00:00:00

abstract：:Saccharomyces cerevisiae was transformed with a genomic library from Penicillium purpurogenum, and an endoxylanase-producing yeast clone (named 44A) that grows on xylose or xylan as sole carbon source was isolated. This yeast synthesizes xynA mRNA and secretes endoxylanase A to culture media when grown on xylan or xyl...

journal_title：FEMS microbiology letters

authors： Chávez R,Navarro C,Calderón I,Peirano A,Bull P,Eyzaguirre J

更新日期：2002-07-02 00:00:00

abstract：:The aim of this study was to evaluate the ability of a nested PCR system to detect Salmonella senftenberg in raw oysters. The specific primers of the PCR were derived from the invA gene sequence, essential for Salmonella invasiveness into epithelial cells. First, for the extraction of DNA, four methods (guanidine isot...

journal_title：FEMS microbiology letters

authors： Vázquez-Novelle MD,Pazos AJ,Abad M,Sánchez JL,Pérez-Parallé ML

更新日期：2005-02-01 00:00:00

abstract：:Multidrug efflux pumps contribute to multiple antibiotic resistance in Pseudomonas aeruginosa. Pump expression usually has been quantified by Western blotting. Quantitative real-time polymerase chain reaction has been developed to measure mRNA expression for genes of interest. Whether this method correlates with pump ...

journal_title：FEMS microbiology letters

authors： Yoneda K,Chikumi H,Murata T,Gotoh N,Yamamoto H,Fujiwara H,Nishino T,Shimizu E

更新日期：2005-02-01 00:00:00

abstract：:The major heat shock proteins from Thiobacillus ferrooxidans were identified as DnaK and GroEL equivalents by Western blotting and analysis of the N-terminal amino acid sequence of spots isolated from dried 2-D polyacrylamide electrophoresis gels. The T. ferrooxidans chaperonins showed 70% and 80% identity with the Es...

journal_title：FEMS microbiology letters

authors： Varela P,Jerez CA

更新日期：1992-11-01 00:00:00

abstract：:Two variants have been isolated from the wild-type Azospirillum lipoferum strain 4B. The first variant, 4V(I), spontaneously emerged from the wild-type at frequencies in the order of 10(-4) to 10(-3) per cell generation. Compared to the wild-type, the 4V(I) variant gained (production of a carotenoid-like pigment, assi...

journal_title：FEMS microbiology letters

authors： Alexandre G,Bally R

更新日期：1999-05-15 00:00:00

abstract：:Xanthomonas oryzae pv. oryzae is an important plant pathogen which causes bacterial blight of rice. To facilitate genome studies of this bacterium, we have constructed a bacterial artificial chromosome (BAC) library of strain MAFF 311018. It consisted of 750 clones representing 16 genome equivalents, and had an insert...

journal_title：FEMS microbiology letters

authors： Ochiai H,Inoue Y,Hasebe A,Kaku H

更新日期：2001-06-12 00:00:00

abstract：:The alternative sigma factor sigmaH has been shown to play an important role in stress response and morphological differentiation in Streptomyces coelicolor. Its gene, sigH, is located in an operon with the gene encoding proposed anti-sigma factor UshX, and one of the promoters directing expression of the operon is de...

journal_title：FEMS microbiology letters

authors： Sevcikova B,Kormanec J

更新日期：2002-04-09 00:00:00