Construction of scrA::lacZ gene fusions to investigate regulation of the sucrose PTS of Streptococcus mutans.

abstract：:For the establishment of a diagnostic tool for mycobacterial species, a part of the dnaA gene was amplified and sequenced from clinically relevant 27 mycobacterial species as well as 49 clinical isolates. Sequence variability in the amplified segment of the dnaA gene allowed the differentiation of all species except f...

journal_title：FEMS microbiology letters

authors： Mukai T,Miyamoto Y,Yamazaki T,Makino M

更新日期：2006-01-01 00:00:00

abstract：:The 0104 antigen (lipopolysaccharide, LPS) of Escherichia coli has an acidic O specific polysaccharide. From the aqueous phase of a phenol water extraction of E. coli O104: K-, a fraction was obtained by ultracentrifugation and Cetavlon precipitation of the supernatant, which was enriched in long-chain LPS. Compositio...

journal_title：FEMS microbiology letters

authors： Kogan G,Jann B,Jann K

更新日期：1992-03-01 00:00:00

abstract：:Two thermostable phytases were identified from Thai isolates of Aspergillus japonicus BCC18313 (TR86) and Aspergillus niger BCC18081 (TR170). Both genes of 1404 bp length, coding for putative phytases of 468 amino acid residues, were cloned and transferred into Pichia pastoris. The recombinant phytases, r-PhyA86 and r...

journal_title：FEMS microbiology letters

authors： Promdonkoy P,Tang K,Sornlake W,Harnpicharnchai P,Kobayashi RS,Ruanglek V,Upathanpreecha T,Vesaratchavest M,Eurwilaichitr L,Tanapongpipat S

更新日期：2009-01-01 00:00:00

abstract：:Escherichia coli mutant MM52 (secA(ts)) was transformed with a cosmid library from Staphylococcus carnosus, and a recombinant cosmid (pBO23) allowing growth at the non-permissive temperature (42 degrees C) was isolated. pBO23 also restored the growth defects of E. coli mutants IQ85 (secY(ts)) and IT41 (lep(ts)). Nucle...

journal_title：FEMS microbiology letters

authors： Overhoff-Freundlieb B,Freudl R

更新日期：1991-11-15 00:00:00

abstract：:Aspergillus niger represents a promising host for the expression of recombinant proteins, but only a few expression systems are available for this organism. In this study, the inducible catalase promoter (PcatR) from A. niger was characterized. For this, constructs were developed and checked for the expression of the ...

journal_title：FEMS microbiology letters

authors： Sharma R,Katoch M,Govindappa N,Srivastava PS,Sastry KN,Qazi GN

更新日期：2012-02-01 00:00:00

abstract：:The expression of the rbp genes, which encode RNA-binding proteins with a single RNA-recognition motif and a glycine-rich sequence, is known to increase at low temperature in cyanobacteria. We previously showed that their regulation involved both transcription and mRNA stability. In the present study, various reporter...

journal_title：FEMS microbiology letters

authors： Ehira S,Ohmori M,Sato N

更新日期：2005-10-01 00:00:00

abstract：:Staphylococcus aureus lipase (SAL) is known to possess broad substrate specificity for triacylglycerides. We found that a sub-minimum inhibitory concentration of farnesol (1000 mg L(-1)) inhibits this lipase activity on a Mueller-Hinton agar containing 1% Tween substrates. A quantitative lipase assay using p-nitrophen...

journal_title：FEMS microbiology letters

authors： Kuroda M,Nagasaki S,Ito R,Ohta T

更新日期：2007-08-01 00:00:00

abstract：:Despite the large number of techniques available for the transformation of bacteria, several species are still resistant to the introduction of foreign DNA. Oenococcus oeni are among the organisms that are particularly refractory to transformation. However, conjugal experiments from Lactococcus lactis to O. oeni with ...

journal_title：FEMS microbiology letters

authors： Beltramo C,Oraby M,Bourel G,Garmyn D,Guzzo J

更新日期：2004-07-01 00:00:00

abstract：:In Bacillus subtilis, the yoxA and dacC genes were proposed to form an operon. The yoxA gene was overexpressed in Escherichia coli and its product fused to a polyhistidine tag was purified. An aldose-1-epimerase or mutarotase activity was measured with the YoxA protein that we propose to rename as GalM by analogy with...

journal_title：FEMS microbiology letters

authors： Duez C,Zervosen A,Teller N,Melkonian R,Banzubazé E,Bouillenne F,Luxen A,Frère JM

更新日期：2009-11-01 00:00:00

abstract：:Mycoplasma mycoides subsp. mycoides (Mmm) strain Afadé had previously been shown to undergo spontaneous phase variations between an opaque capsulated variant and a translucent (TR) variant devoid of a capsule but able to secrete cell-free exopolysaccharides. This phase variation is associated with an ON/OFF genetic sw...

journal_title：FEMS microbiology letters

authors： Gaurivaud P,Lakhdar L,Le Grand D,Poumarat F,Tardy F

更新日期：2014-10-01 00:00:00

abstract：:Spores of Bacillus species have novel properties, which allow them to lie dormant for years and then germinate under favourable conditions. In the current work, the role of a key metabolic integrator, coenzyme A (CoA), in redox regulation of growing cells and during spore formation in Bacillus megaterium and Bacillus ...

journal_title：FEMS microbiology letters

authors： Zhyvoloup A,Yu BYK,Baković J,Davis-Lunn M,Tossounian MA,Thomas N,Tsuchiya Y,Peak-Chew SY,Wigneshweraraj S,Filonenko V,Skehel M,Setlow P,Gout I

更新日期：2020-12-22 00:00:00

abstract：:Shigella flexneri, which causes shigellosis in humans, evolved from Escherichia coli. The sequencing of Shigella genomes has revealed that a large number of insertion sequence (IS) elements (over 200 elements) reside in the genome. Although the presence of these elements has been noted previously and summarized, more ...

journal_title：FEMS microbiology letters

authors： Zaghloul L,Tang C,Chin HY,Bek EJ,Lan R,Tanaka MM

更新日期：2007-12-01 00:00:00

abstract：:Streptococcus pneumoniae strain JNR.7/87 is a highly virulent, type 4 encapsulated Gram-positive bacterium whose transformability has not been tested previously, and whose genome is currently being sequenced. The strain was transformed at very low efficiency by addition of exogenous competence-stimulating peptide: How...

journal_title：FEMS microbiology letters

authors： Bricker AL,Camilli A

更新日期：1999-03-15 00:00:00

abstract：:Arbuscular mycorrhizal fungi (AMF) belong to phylum Glomeromycota, an early divergent fungal lineage forming symbiosis with plant roots. Many reports have documented that bacteria are intimately associated with AMF mycelia in the soil. However, the role of these bacteria remains unclear and their diversity within intr...

journal_title：FEMS microbiology letters

authors： Iffis B,St-Arnaud M,Hijri M

更新日期：2014-09-01 00:00:00

abstract：:Burkholderia pseudomallei, a Gram-negative bacterium is found in the soil and water, mainly in Southeast Asia and Northern Australia. It is responsible for melioidosis in human and animals. The bacteria produce several potential virulent factors such as extracellular protease, hemolysin, lipase and lecithinase. The is...

journal_title：FEMS microbiology letters

authors： Lee MA,Liu Y

更新日期：2000-11-01 00:00:00

abstract：:Mycelium of Pleurotus ostreatus var. florida with a decreased growth rate contained seven double-stranded RNA segments and isometrical virus particles with diameters of 24 and 30 nm. Mycelium with a normal growth rate lacked dsRNA. Protoclones from virus-containing mycelium contained one to seven of these dsRNA segmen...

journal_title：FEMS microbiology letters

authors： van der Lende TR,Harmsen MC,Go SJ,Wessels JG,Wessel JG

更新日期：1995-01-01 00:00:00

abstract：:Helicobacter pullorum and Campylobacter lari are rarely isolated from humans with acute enteritis. Hitherto the two species could only be identified by genotypic techniques. Gas liquid chromatography of whole cell fatty acid extracts is described as the first phenotypic method for discrimination of the two species. Ch...

journal_title：FEMS microbiology letters

authors： Steinbrueckner B,Haerter G,Pelz K,Burnens A,Kist M

更新日期：1998-11-15 00:00:00

abstract：:We present evidence that glycine betaine (betaine) which was synthesized from choline was excreted and reaccumulated in osmoregulating cells of Escherichia coli. Choline which was accumulated in bet mutants defective in betaine synthesis was shown to be excreted in response to betaine uptake. Our data suggest that E. ...

journal_title：FEMS microbiology letters

authors： Lamark T,Styrvold OB,Strøm AR

更新日期：1992-09-15 00:00:00

abstract：:Five independent collections, comprising a total of 34 clones encoding cellulases, hemicellulases and cell surface proteins of Clostridium thermocellum, were searched for overlapping or contiguous DNA fragments. The clones were hybridized to large genomic restriction fragments separated by pulse-field electrophoresis....

journal_title：FEMS microbiology letters

authors： Guglielmi G,Béguin P

更新日期：1998-04-01 00:00:00

abstract：:D-(-)-3-Hydroxybutyrate (3HB) oligomer hydrolase was purified from Paracoccus denitrificans. The enzyme was a monomeric protein with an approximate molecular mass of 31 kDa. The isoelectric point of the enzyme was 5.2. Optimum temperature and pH were 35-40 degrees C and 8.0, respectively. The enzyme activity was not a...

journal_title：FEMS microbiology letters

authors： Ueda S,Sano K,Gao D,Tomihari N,Yamane T,Endo I

更新日期：2002-01-10 00:00:00

abstract：:Saccharomyces cerevisiae genes encoding functions necessary for inhibition by the Pseudomonas syringae pv. syringae cyclic lipodepsipeptide, syringomycin-E, were identified by mutant analyses. Syringomycin-E-resistant mutants were isolated, shown to contain single recessive mutations, and divided into eight gene compl...

journal_title：FEMS microbiology letters

authors： Takemoto JY,Yu Y,Stock SD,Miyakawa T

更新日期：1993-12-15 00:00:00

abstract：:The enterobacterium Erwinia amylovora is the causal agent of fire blight. This study presents the analysis of the complete genome of phage PhiEaH1, isolated from the soil surrounding an E. amylovora-infected apple tree in Hungary. Its genome is 218 kb in size, containing 244 ORFs. PhiEaH1 is the second E. amylovora in...

journal_title：FEMS microbiology letters

authors： Meczker K,Dömötör D,Vass J,Rákhely G,Schneider G,Kovács T

更新日期：2014-01-01 00:00:00

abstract：:This study was initiated to establish whether inhibition of growth of yeasts by medium-chain fatty acids resembled that caused by weak-acid preservatives or uncouplers. Unlike sorbic acid and 2,4-dinitrophenol, decanoic acid caused rapid cell death at its inhibitory concentration. This suggested a mode of action by me...

journal_title：FEMS microbiology letters

authors： Stratford M,Anslow PA

更新日期：1996-08-15 00:00:00

abstract：:Legionella pneumophila, the aetiologic agent of legionnaires' disease, contains a single, monopolar flagellum which is composed of one major subunit, the FlaA protein. Expression studies using a reporter gene fusion of the flaA promoter with the luxAB gene revealed that the flaA expression is not only temperature regu...

journal_title：FEMS microbiology letters

authors： Heuner K,Brand BC,Hacker J

更新日期：1999-06-01 00:00:00

abstract：:A class 1 integron was detected in a single multidrug-resistant strain of enterotoxigenice Escherichia coli (ETEC) O159 after examination of 23 clinical E. coli isolates. This isolate was resistant to streptomycin, kanamycin, gentamicin, chloramphenicol and ampicillin. Sequencing of the class 1 integron identified thr...

journal_title：FEMS microbiology letters

authors： Ahmed AM,Shimamoto T

更新日期：2004-06-15 00:00:00

abstract：:Recently, a novel verocytotoxin named VT2y was described which belongs to the STx family and is produced by Escherichia coli isolated from domestic poultry with swollen head syndrome (SHS). The VT2y toxin induced apoptosis in Vero, HeLa, CHO, CEF (primary chicken embryo fibroblast) and PCK (primary chicken kidney) cel...

journal_title：FEMS microbiology letters

authors： Salvadori MR,Yamada AT,Yano T

更新日期：2001-04-01 00:00:00

abstract：:This study characterizes the glucomannan utilization operon (gmuBACDREFG, formerly ydhMNOPQRST) of Bacillus subtilis. Transcription of the operon is induced by konjac glucomannan and requires the last mannanase gene (gmuG). Cellobiose and mannobiose, possible degradation products of glucomannan by GmuG, are strong ind...

journal_title：FEMS microbiology letters

authors： Sadaie Y,Nakadate H,Fukui R,Yee LM,Asai K

更新日期：2008-02-01 00:00:00

abstract：:The polymerase chain reaction (PCR)-based procedures of randomly amplified polymorphic DNA (RAPD) and repetitive element (RE)-based PCR were used to amplify total DNA prepared from each of 62 clinical Serratia marcescens isolates. Three different random primers, designated 1060, 1254 and 1283, were used individually i...

journal_title：FEMS microbiology letters

authors： Patton TG,Katz S,Sobieski RJ,Crupper SS

更新日期：2001-01-01 00:00:00

abstract：:Leflunomide (LFM) is a novel anti-inflammatory and immunosuppressive drug, and inhibits the growth of cytokine-stimulated lymphoid cells in vitro. The effect of LFM on haploid and diploid cells of Saccharomyces cerevisiae was investigated to elucidate the molecular mechanism of action of the drug. Using a halo assay, ...

journal_title：FEMS microbiology letters

authors： Fujimura H

更新日期：2000-10-01 00:00:00

abstract：:The photoinduced bactericidal capacity of TiO(2) based films was evaluated, using as model organism Pseudomonas aeruginosa. Thin films were obtained by spray pyrolysis; they included undoped, Cu doped, and Al doped TiO(2). Scanning electron microscopy was used to observe the final effect of the irradiated films upon t...

journal_title：FEMS microbiology letters

authors： Amézaga-Madrid P,Nevárez-Moorillón GV,Orrantia-Borunda E,Miki-Yoshida M

更新日期：2002-06-04 00:00:00