Analysis of the Spirochaeta aurantia flaA gene and transcript.

abstract：:Rhizobium leguminosarum bv. trifolii rosR gene encodes a transcriptional regulator involved in the positive regulation of exopolysaccharide synthesis. Transcription of rosR is directed by two promoters, distal P1 and proximal P2, of different strengths. We demonstrated that rosR P1 functions as the main promoter and, ...

journal_title：FEMS microbiology letters

authors： Janczarek M,Skorupska A

更新日期：2009-02-01 00:00:00

abstract：:Autotrophic fixation of carbon dioxide into cellular carbon occurs via several pathways but quantitatively, the Calvin-Benson-Bassham cycle is the most important. CbbR regulates the expression of the cbb genes involved in CO2 fixation via the Calvin-Benson-Bassham cycle in a number of autotrophic bacteria. A gene pote...

journal_title：FEMS microbiology letters

authors： Esparza M,Jedlicki E,Dopson M,Holmes DS

更新日期：2015-08-01 00:00:00

abstract：:The low heat resistance (D100 = 0.554 min, z = 13.4 degrees C) of dormant lysozyme-sensitized spores of Bacillus sphaericus 9602 was correlated with a low protoplast wet density (1.305 g/ml) equivalent to a high protoplast water content (61.0%, wet weight basis). These values for these unusual spores were consistent w...

journal_title：FEMS microbiology letters

authors： Beaman TC,Pankratz HS,Gerhardt P

更新日期：1989-03-01 00:00:00

abstract：:Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) represents a simple reliable approach for rapid bacterial identification based on specific peptide/protein fingerprints. However, cell-wall characteristics of mycobacterial species, and their well known stability, complicate MA...

journal_title：FEMS microbiology letters

authors： Balážová T,Makovcová J,Šedo O,Slaný M,Faldyna M,Zdráhal Z

更新日期：2014-04-01 00:00:00

abstract：:In Bordetella pertussis virulence-associated genes, including adenylate cyclase toxin (Cya), are coordinately regulated in response to environmental signals by proteins coded by the bvg-locus. We have constructed cya-lac fusions in Escherichia coli and have shown that the cya operon is not expressed in E. coli, neithe...

journal_title：FEMS microbiology letters

authors： Goyard S,Ullmann A

更新日期：1991-01-15 00:00:00

abstract：:Both ThyA and ThyX proteins catalyze the transfer of the methyl group from methylenetetrahydrofolate (CH(2) H(4) -folate) to dUMP, forming dTMP. To estimate the relative steady state expression levels of ThyA and ThyX, Western blot analysis was performed using ThyA or ThyX antiserum on total protein from the wild-type...

journal_title：FEMS microbiology letters

authors： Cho S,Yang S,Rhie H

更新日期：2012-03-01 00:00:00

abstract：:To elucidate the molecular mechanism by which the MexR repressor regulates expression of the MexAB-OprM efflux pump, we investigated MexR and the mexR-mexA intergenic DNA (mexOP) interaction, and transcription of the mexA-lacZ reporter gene containing different lengths of mexOP. Homogeneously purified MexR bound speci...

journal_title：FEMS microbiology letters

authors： Saito K,Eda S,Maseda H,Nakae T

更新日期：2001-02-05 00:00:00

abstract：:The Saccharomyces sensu stricto genus contains many species that are industrially important for fermentation of wines, beers and ales. The molecular characterization of the genomes of yeasts involved in these processes reveals that the majority arose from interspecific hybridization between two and sometimes three yea...

journal_title：FEMS microbiology letters

authors： Querol A,Bond U

更新日期：2009-04-01 00:00:00

abstract：:Cytoplasmic precursors of the peptidoglycan biosynthetic pathway were purified from vancomycin-treated, glycopeptide-sensitive and -resistant strains of Enterococcus faecium. Resistance was due to production of a modified precursor, UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-lactate, where lactate was identified on the basi...

journal_title：FEMS microbiology letters

authors： Messer J,Reynolds PE

更新日期：1992-07-01 00:00:00

abstract：:A growing body of evidence implicates streptococcal and staphylococcal superantigens in the development of psoriasis, atopic dermatitis and Kawasaki syndrome. In each of these illnesses, an abnormal state of immunologic activity is observed. Superantigens, which have a unique ability to activate large numbers of lymph...

journal_title：FEMS microbiology letters

authors： Yarwood JM,Leung DY,Schlievert PM

更新日期：2000-11-01 00:00:00

abstract：:The spirochaete, Serpulina pilosicoli, is the agent of intestinal spirochaetosis, a diarrhoeal disease of humans and other species. By mechanisms as yet unknown, large numbers of these spirochaetes intimately attach to the colonic mucosa by one cell end. In some infected individuals, the spirochaetes may invade the la...

journal_title：FEMS microbiology letters

authors： Hartland EL,Mikosza AS,Robins-Browne RM,Hampson DJ

更新日期：1998-08-01 00:00:00

abstract：:A Gram-negative, nonmotile and rod-shaped bacterial strain was isolated from the rhizosphere of Platycodon grandiflorum in a study of bacterial diversity, and its taxonomic position was investigated by a genotypic and phenotypic analysis. This isolate, designated as DR-f4, grew at 4-30 degrees C (optimally at 20-25 de...

journal_title：FEMS microbiology letters

authors： Kim BC,Lee KH,Kim MN,Lee J,Shin KS

更新日期：2010-08-01 00:00:00

abstract：:Using primers corresponding to conserved regions of the bacterial regulatory gene fur, a homologue of this gene from the genome of Rhizobium leguminosarum biovar viciae, the nitrogen-fixing symbiont of peas, was isolated and sequenced. The fur gene is normally expressed constitutively, independent of the presence of F...

journal_title：FEMS microbiology letters

authors： de Luca NG,Wexler M,Pereira MJ,Yeoman KH,Johnston AW

更新日期：1998-11-15 00:00:00

abstract：:To understand their mechanism of antimicrobial activity against Gram-negative bacteria, ostrich beta-defensins, ostricacins-1 and 2 (Osp-1 and Osp-2), were compared with those of sheep myeloid antimicrobial peptide (SMAP)-29 and human neutrophil peptide (HNP)-1, well-characterized sheep alpha-helical and human alpha-d...

journal_title：FEMS microbiology letters

authors： Sugiarto H,Yu PL

更新日期：2007-05-01 00:00:00

abstract：:Uncultured archaeal anaerobic methanotrophs (ANMEs) are known to operate the anaerobic oxidation of methane process, an important sink for the greenhouse gas methane in natural environments. In this study, we designed 16S rRNA gene-specific primers for each of the phylogenetic groups of ANMEs (ANME-1, Guaymas Basin hy...

journal_title：FEMS microbiology letters

authors： Miyashita A,Mochimaru H,Kazama H,Ohashi A,Yamaguchi T,Nunoura T,Horikoshi K,Takai K,Imachi H

更新日期：2009-08-01 00:00:00

abstract：:The 181 251 bp accessory plasmid pSmeSM11b of Sinorhizobium meliloti strain SM11, belonging to a dominant indigenous S. meliloti subpopulation identified during a long-term field release experiment, was sequenced. This plasmid has 166 coding sequences (CDSs), 42% of which encode proteins with homology to proteins of k...

journal_title：FEMS microbiology letters

authors： Stiens M,Schneiker S,Pühler A,Schlüter A

更新日期：2007-06-01 00:00:00

abstract：:We screened for a gene that inhibits streptomycin production in Streptomyces griseus when it is introduced on a high-copy-number plasmid pIJ702, and obtained a plasmid pKM545. The introduction of pKM545 abolished streptomycin production on all media tested including YMP-sugar and Nutrient broth. S1 protection analysis...

journal_title：FEMS microbiology letters

authors： Takano H,Toriumi N,Hirata M,Amano T,Ohya T,Shimada R,Kusada H,Amano S,Matsuda K,Beppu T,Ueda K

更新日期：2016-07-01 00:00:00

abstract：:Protein interactions among RNA polymerase small subunits from the archaeon Methanococcus jannaschii were investigated using affinity pulldown assays in pairwise and higher-order combinations. In the most extensive study of archaeal RNA polymerase subunit interactions to date, including 37 pairs of proteins, 10 ternary...

journal_title：FEMS microbiology letters

authors： Best AA,Olsen GJ

更新日期：2001-02-05 00:00:00

abstract：:We isolated a fungal strain HS-1 utilizing either ethylene glycol dibenzoate or ethyl benzoate as sole source of carbon and energy, and identified it as Aspergillus nomius, based on morphological and rDNA analyses. An enzyme hydrolyzing the esters was purified from the culture supernatant of the strain to an electroph...

journal_title：FEMS microbiology letters

authors： Uchida H,Kurakata Y,Sawamura H,Inamura N,Kotani T,Uwajima T

更新日期：2003-06-06 00:00:00

abstract：:The classical monomeric autotransporters are ubiquitously used by Gram-negative bacteria to export virulence and colonization factors to their cell surface or into their surroundings. They are expressed as monomeric proteins that pass the inner and outer membrane in two consecutive steps facilitated by the Sec translo...

journal_title：FEMS microbiology letters

authors： van Ulsen P,Zinner KM,Jong WSP,Luirink J

更新日期：2018-09-01 00:00:00

abstract：:Coagulase-negative staphylococci (CoNS) are common bacterial colonizers of the human skin. They are often involved in nosocomial infections due to biofilm formation in indwelling medical devices. While biofilm formation has been extensively studied in Staphylococcus epidermidis, little is known regarding other CoNS sp...

journal_title：FEMS microbiology letters

authors： Oliveira F,Lima CA,Brás S,França Â,Cerca N

更新日期：2015-10-01 00:00:00

abstract：:The adhesion-promoting proteins (APP) (molecular mass approx. 300 kDa), which promote adhesion of Streptococcus mutans JBP (serotype c) to hydroxyapatite, were isolated from human submandibular-sublingual (SMSL) saliva by gel filtration on a Trisacryl GP2000 M column. The effects of hexoses, pentoses, methyl-pentoses,...

journal_title：FEMS microbiology letters

authors： Kishimoto E,Hay DI,Gibbons RJ

更新日期：1991-12-15 00:00:00

abstract：:The phyCg gene encoding a new phytase from Citrobacter gillenii was optimized, synthesized, cloned and expressed in Pichia pastoris. Analysis of the amino acid sequence of the enzyme showed that it belongs to the histidine acid phosphatase family. The amino acid sequence of the PhyCg phytase has the highest homology (...

journal_title：FEMS microbiology letters

authors： Tkachenko AA,Kalinina AN,Borshchevskaya LN,Sineoky SP,Gordeeva TL

更新日期：2020-12-21 00:00:00

abstract：:When bacterial cells are subjected to stress, cellular and population characteristics can change significantly. Rapid acquisition of these properties of cells in their native physiological state is necessary in order to precisely interpret their survival modes. The multiparametric analytical capability of flow cytomet...

journal_title：FEMS microbiology letters

authors： Sachidanandham R,Gin KY

更新日期：2009-01-01 00:00:00

abstract：:Streptomyces netropsis SD-07, the producer of novel polyene macrolide antifungal antibiotics, was isolated from soil. For the investigation of the functions of its biosynthesis genes and regulation mechanisms, a genetic operating system is necessary. In this study, we successfully transferred the plasmid DNA of pSET15...

journal_title：FEMS microbiology letters

authors： Wang XK,Jin JL

更新日期：2014-08-01 00:00:00

abstract：:We have investigated the mechanisms used by Trichomonas vaginalis to damage cellular membranes, using human erythrocytes as target cells. Haemolysis is a contact- and temperature-dependent phenomenon, and is inhibited in 4 mM EGTA. Osmotic protection experiments using carbohydrates with different molecular diameters a...

journal_title：FEMS microbiology letters

authors： Fiori PL,Rappelli P,Rocchigiani AM,Cappuccinelli P

更新日期：1993-05-01 00:00:00

abstract：:Mycelium of Pleurotus ostreatus var. florida with a decreased growth rate contained seven double-stranded RNA segments and isometrical virus particles with diameters of 24 and 30 nm. Mycelium with a normal growth rate lacked dsRNA. Protoclones from virus-containing mycelium contained one to seven of these dsRNA segmen...

journal_title：FEMS microbiology letters

authors： van der Lende TR,Harmsen MC,Go SJ,Wessels JG,Wessel JG

更新日期：1995-01-01 00:00:00

abstract：:The polyhydroxyalkanoic acid (PHA) synthase gene (phaCAc) of a species of Acinetobacter isolated from an activated sludge treatment plant was cloned by heterologous complementation in a poly-beta-hydroxybutyrate (PHB) negative mutant of Alcaligenes eutrophus. Nucleotide sequence analysis of phaCAc revealed an open rea...

journal_title：FEMS microbiology letters

authors： Schembri MA,Bayly RC,Davies JK

更新日期：1994-05-01 00:00:00

abstract：:Expression of the dimethylsulfoxide respiratory (dor) operon of Rhodobacter is regulated by oxygen, light intensity and availability of substrate. Since dimethylsulfoxide reductase contains a pterin molybdenum cofactor, the role of molybdate in the regulation of dor operon expression was investigated. In this report w...

journal_title：FEMS microbiology letters

authors： Solomon PS,Shaw AL,Young MD,Leimkuhler S,Hanson GR,Klipp W,McEwan AG

更新日期：2000-09-15 00:00:00

abstract：:An Acremonium chrysogenum strain improvement program based on the transformation with cephalosporin biosynthetic genes was carried out to enhance cephalosporin C production. Best results were obtained with cefEF and cefG genes, selecting transformants with increased cephalosporin C production and lower accumulation of...

journal_title：FEMS microbiology letters

authors： Rodríguez-Sáiz M,Lembo M,Bertetti L,Muraca R,Velasco J,Malcangi A,de la Fuente JL,Barredo JL

更新日期：2004-06-01 00:00:00