Analysis of CcpA mutations defective in carbon catabolite repression in Bacillus megaterium.

Abstract:

:Five mutations in ccpA of Bacillus megaterium with impaired functions were analysed for carbon catabolite repression. The phenotypes support the hypothesis that CcpA assumes a PurR/LacI fold. The completely inactive mutants CcpA119GE and CcpA326am cause alterations which are incompatible with that fold. A mutation with reduced activity, CcpA81GE, affects a site that would be partially surface exposed and may interfere with structure formation or cofactor binding. A mutation in the putative hinge alpha-helix, CcpA52AE, is negative transdominant over wild-type ccpA. The mutant CcpA38am is inactive, although reduced amounts of wild-type size protein are produced.

journal_name

FEMS Microbiol Lett

authors

Kraus A,Hillen W

doi

10.1111/j.1574-6968.1997.tb10485.x

subject

Has Abstract

pub_date

1997-08-01 00:00:00

pages

221-6

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(97)00264-4

journal_volume

153

pub_type

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