Abstract:
:Five mutations in ccpA of Bacillus megaterium with impaired functions were analysed for carbon catabolite repression. The phenotypes support the hypothesis that CcpA assumes a PurR/LacI fold. The completely inactive mutants CcpA119GE and CcpA326am cause alterations which are incompatible with that fold. A mutation with reduced activity, CcpA81GE, affects a site that would be partially surface exposed and may interfere with structure formation or cofactor binding. A mutation in the putative hinge alpha-helix, CcpA52AE, is negative transdominant over wild-type ccpA. The mutant CcpA38am is inactive, although reduced amounts of wild-type size protein are produced.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Kraus A,Hillen Wdoi
10.1111/j.1574-6968.1997.tb10485.xsubject
Has Abstractpub_date
1997-08-01 00:00:00pages
221-6issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(97)00264-4journal_volume
153pub_type
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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更新日期:1993-11-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2005.00099.x
更新日期:2006-03-01 00:00:00