Confirmation by using mutant strains that the membrane-bound H(+)-ATPase is the major source of non-linear dielectricity in Saccharomyces cerevisiae.

Abstract:

:Non-linear dielectric spectroscopy is a novel technique for determining the activity of (predominantly) membranous enzymes as their ability to generate harmonics when excited with a sinusoidal electrical field. In washed suspensions of yeast cells, the ability to generate harmonics is inhibited by low concentrations of sodium vanadate, suggesting that the vanadate-sensitive H(+)-ATPase is the major source of the non-linear dielectricity. This conclusion is greatly strengthened by the demonstration herein that the generation of harmonics by a strain containing a vandate-resistant H(+)-ATPase is also highly resistant to sodium metavanadate.

journal_name

FEMS Microbiol Lett

authors

Woodward AM,Kell DB

doi

10.1016/0378-1097(91)90401-u

subject

Has Abstract

pub_date

1991-11-01 00:00:00

pages

91-5

issue

1

eissn

0378-1097

issn

1574-6968

journal_volume

68

pub_type

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