Fatty acids synthesized by oral treponemes in chemically defined media.

abstract：:A [Fe]-hydrogenase gene (hydA) was cloned from Clostridium paraputrificum M-21 in Escherichia coli using a conserved DNA sequence of clostridial hydrogenase genes amplified by PCR as the probe. The hydA gene consisted of an open reading frame of 1749 bp encoding 582 amino acids with an estimated molecular mass of 64,5...

journal_title：FEMS microbiology letters

authors： Morimoto K,Kimura T,Sakka K,Ohmiya K

更新日期：2005-05-15 00:00:00

abstract：:This study focused on the alanine racemase gene (alr-2), which is involved in the synthesis of d-alanine that forms the backbone of the cell wall. A stable alr-2 knockout mutant of Aeromonas hydrophila HBNUAh01 was constructed. When the mutant was supplemented with d-alanine, growth was unaffected; deprivation of d-al...

journal_title：FEMS microbiology letters

authors： Liu D,Zhang L,Xue W,Wang Y,Ju J,Zhao B

更新日期：2015-07-01 00:00:00

abstract：:Aspergillus parasiticus (255), a non-toxigenic isolate showed the presence of secondary metabolites-aflatoxins (B1, B2, G1, G2) when grown in yeast extract sucrose media but not in basal media, thus demonstrating its toxigenic potential. Native PAGE of the crude protein isolated at different growth periods of A. paras...

journal_title：FEMS microbiology letters

authors： Shashidhar J,Sashidhar RB,Deshpande V

更新日期：2005-10-01 00:00:00

abstract：:We have cloned and sequenced the rfaH gene from Salmonella enterica serovar Typhi strain Ty2. The gene showed a high degree of similarity to the rfaH genes from Escherichia coli K-12 and S. enterica serovar Typhimurium. A rfaH mutant was constructed by site-directed mutagenesis. This mutant produced a rough lipopolysa...

journal_title：FEMS microbiology letters

authors： Rojas G,Saldías S,Bittner M,Zaldívar M,Contreras I

更新日期：2001-10-16 00:00:00

abstract：:Strains of Salmonella enteritidis expressed a novel porin with a subunit size of 35.5 kDa as shown by SDS-PAGE. This protein was expressed as a major outer membrane protein (MOMP) when grown on nutrient agar, McConkey agar or blood agar, or in Tris-succinate medium; but was only produced in trace amounts when strains ...

journal_title：FEMS microbiology letters

authors： Chart H,Frost JA,Rowe B

更新日期：1993-05-15 00:00:00

abstract：:Bordetella calmodulin-like protein was purified from culture supernatant fluid of B. pertussis, B. parapertussis and B. bronchiseptica by successive chromatography on hydroxyapatite, Toyopearl HW-50F and QAE-Toyopearl 550C columns. The purified calmodulin-like protein appeared to be homogeneous by SDS-polyacrylamide g...

journal_title：FEMS microbiology letters

authors： Nagai M,Endoh M,Danbara H,Nakase Y

更新日期：1994-02-15 00:00:00

abstract：:When Escherichia coli STII was applied to the serosa of the ileum at a concentration of 40 micrograms/ml, an acceleration of the spontaneous motility and a weak contraction were induced 2-3 min later. The induction was not affected by the addition of atropine (10(-6) M), but was abolished by the addition of papaverine...

journal_title：FEMS microbiology letters

authors： Hitotsubashi S,Akagi M,Saitou A,Yamanaka H,Fujii Y,Okamoto K

更新日期：1992-01-15 00:00:00

abstract：:The aim of this study was to understand the pathogenesis of motile aeromonas septicemia caused by an emergent, high virulent Aeromonas hydrophila (vAh) in channel catfish, Ictalurus punctatus Adipose fin clipped catfish were challenged with vAh using a waterborne challenge method, and the distribution of vAh over a ti...

journal_title：FEMS microbiology letters

authors： Zhang D,Moreira GS,Shoemaker C,Newton JC,Xu DH

更新日期：2016-05-01 00:00:00

abstract：:A novel strain of Rhizopus oryzae WPG secretes a noninduced lipase (ROLw) in the culture medium; purified ROLw is a protein of 29 kDa, the 45 N-terminal amino acid residues were sequenced, this sequence is very homologous to Rhizopus delemar lipase (RDL), Rhizopus niveus lipase (RNL) and R. oryzae lipase (ROL29) seque...

journal_title：FEMS microbiology letters

authors： Salah RB,Mosbah H,Fendri A,Gargouri A,Gargouri Y,Mejdoub H

更新日期：2006-07-01 00:00:00

abstract：:Acetobacter spp. are used for industrial vinegar production because of their high ability to oxidize ethanol to acetic acid and high resistance to acetic acid. Two-dimensional gel electrophoretic analysis of a soluble fraction of Acetobacter aceti revealed the presence of several proteins whose production was enhanced...

journal_title：FEMS microbiology letters

authors： Nakano S,Fukaya M,Horinouchi S

更新日期：2004-06-15 00:00:00

abstract：:Carbohydrate-binding components were shown to be present at the surface of Listeria monocytogenes by means of a panel of neoglycoproteins using direct agglutination. These lectin-like components bind on neoglycoproteins bearing D-glucosamine, L-fucosylamine, or para-amino-phenyl-alpha-D-mannopyrannoside residues. The ...

journal_title：FEMS microbiology letters

authors： Cottin J,Loiseau O,Robert R,Mahaza C,Carbonnelle B,Senet JM

更新日期：1990-03-15 00:00:00

abstract：:The response regulator proteins of two-component systems mediate many adaptations of bacteria to their ever-changing environment. Most response regulators are transcription factors that alter the level of transcription of specific sets of genes. Activation of response regulators requires their phosphorylation on a con...

journal_title：FEMS microbiology letters

authors： Ladds JC,Muchová K,Blaskovic D,Lewis RJ,Brannigan JA,Wilkinson AJ,Barák I

更新日期：2003-06-27 00:00:00

abstract：:The major haem component in the black pigment of Porphyromonas gingivalis is the mu-oxo bishaem of iron protoporphyrin IX and formation and cell-surface binding of this haem species is proposed as an extracellular buffer against reactive oxidants [Smalley, J.W. et al. (1998) Biochem. J. 331, 681-685]. P. gingivalis ce...

journal_title：FEMS microbiology letters

authors： Smalley JW,Birss AJ,Silver J

更新日期：2000-02-01 00:00:00

abstract：:A 3.5-kb native plasmid (pND103) was identified in Streptococcus thermophilus ST2-1. Preliminary sequence analysis indicated that pND103 belongs to group I S. thermophilus plasmids. A region of approximately 2 kb appears to contain three components: a plus origin of replication (ori) typical of plasmids that replicate...

journal_title：FEMS microbiology letters

authors： Su P,Jury K,Allison GE,Wong WY,Kim WS,Liu CQ,Vancov T,Dunn NW

更新日期：2002-10-29 00:00:00

abstract：:The majority of microorganisms in natural environments are difficult to cultivate, but their genes can be studied via metagenome libraries. To enhance the chances that these genes become expressed we here report the construction of a broad-host-range plasmid vector (pRS44) for fosmid and bacterial artificial chromosom...

journal_title：FEMS microbiology letters

authors： Aakvik T,Degnes KF,Dahlsrud R,Schmidt F,Dam R,Yu L,Völker U,Ellingsen TE,Valla S

更新日期：2009-06-01 00:00:00

abstract：:Cells of the pel1 mutant of Saccharomyces cerevisiae were found to contain an extremely low content of cardiolipin, a decreased level of phosphatidylcholine and an increased level of phosphatidylinositol. Disruption of the PEL1 gene in cells containing a null mutation in the CHO1 gene was lethal. Despite its putative ...

journal_title：FEMS microbiology letters

authors： Janitor M,Obernauerová M,Kohlwein SD,Subík J

更新日期：1996-06-15 00:00:00

abstract：:Antibiotic therapies are known to disrupt gastrointestinal (GI) bacterial communities. HIV and pathogenic simian immunodeficiency virus (SIV) infections have also been associated with disrupted GI bacterial communities. We administered a combination antibiotic therapy to six SIV-infected rhesus macaques and collected ...

journal_title：FEMS microbiology letters

authors： Zevin AS,Hensley-McBain T,Miller C,Smith E,Langevin S,Klatt NR

更新日期：2017-12-15 00:00:00

abstract：:The wine yeast Saccharomyces cerevisiae is central in the production of aroma compounds during fermentation. Some of the most important yeast-derived aroma compounds produced are esters. The esters ethyl acetate and isoamyl acetate are formed from alcohols and acetyl-CoA in a reaction catalysed by alcohol acetyltransf...

journal_title：FEMS microbiology letters

authors： Cordente AG,Swiegers JH,Hegardt FG,Pretorius IS

更新日期：2007-02-01 00:00:00

abstract：:Helicobacter pullorum and Campylobacter lari are rarely isolated from humans with acute enteritis. Hitherto the two species could only be identified by genotypic techniques. Gas liquid chromatography of whole cell fatty acid extracts is described as the first phenotypic method for discrimination of the two species. Ch...

journal_title：FEMS microbiology letters

authors： Steinbrueckner B,Haerter G,Pelz K,Burnens A,Kist M

更新日期：1998-11-15 00:00:00

abstract：:Acinetobacter sp. strain 20B was isolated based on the ability to utilize dimethyl sulfide as the sole sulfur source. Since strain 20B oxidized indole as well as dimethyl sulfide, indigo production by recombinant Escherichia coli clones carrying Acinetobacter DNA was used as a selection for cloning genes encoding dime...

journal_title：FEMS microbiology letters

authors： Horinouchi M,Kasuga K,Nojiri H,Yamane H,Omori T

更新日期：1997-10-01 00:00:00

abstract：:A polymerase chain reaction (PCR) amplification of 16S rDNA was developed to identify spirochaetes recovered from cases of intestinal spirochaetosis in humans and pigs; these bacteria belong to a distinct genetic group of spirochaetes, with the proposed name 'Anguillina coli'. The PCR incorporated a universal eubacter...

journal_title：FEMS microbiology letters

authors： Park NY,Chung CY,McLaren AJ,Atyeo RF,Hampson DJ

更新日期：1995-01-15 00:00:00

abstract：:A class 1 integron was detected in a single multidrug-resistant strain of enterotoxigenice Escherichia coli (ETEC) O159 after examination of 23 clinical E. coli isolates. This isolate was resistant to streptomycin, kanamycin, gentamicin, chloramphenicol and ampicillin. Sequencing of the class 1 integron identified thr...

journal_title：FEMS microbiology letters

authors： Ahmed AM,Shimamoto T

更新日期：2004-06-15 00:00:00

abstract：:Homologs of the Escherichia coli oxyR gene were identified in several Erwinia species, using a combination of PCR and Southern hybridization analysis. The oxyR gene from Erwinia carotovora was isolated on a cosmid clone and characterized. The gene and deduced gene product shared high level sequence identity with their...

journal_title：FEMS microbiology letters

authors： Calcutt MJ,Lewis MS,Eisenstark A

更新日期：1998-10-15 00:00:00

abstract：:Saccharomyces cerevisiae was transformed with a genomic library from Penicillium purpurogenum, and an endoxylanase-producing yeast clone (named 44A) that grows on xylose or xylan as sole carbon source was isolated. This yeast synthesizes xynA mRNA and secretes endoxylanase A to culture media when grown on xylan or xyl...

journal_title：FEMS microbiology letters

authors： Chávez R,Navarro C,Calderón I,Peirano A,Bull P,Eyzaguirre J

更新日期：2002-07-02 00:00:00

abstract：:The c-type cytochrome (OmcB) and the multicopper protein (OmpB) required for Fe(III) oxide reduction by Geobacter sulfurreducens were predicted previously to be outer membrane proteins, but it is not clear whether they are positioned in a manner that permits the interaction with Fe(III). Treatment of whole cells with ...

journal_title：FEMS microbiology letters

authors： Qian X,Reguera G,Mester T,Lovley DR

更新日期：2007-12-01 00:00:00

abstract：:A bacterial isolate, tentatively identified as Pseudomonas sp. strain TR3, was found to utilize the diaryl ester phenylbenzoate as sole source of carbon and energy. This strain has the ability to productively degrade phenylbenzoate and some substituted derivatives by a catabolic sequence which was characterized bioche...

journal_title：FEMS microbiology letters

authors： Reich T,Schmidt S,Fortnagel P

更新日期：1999-07-15 00:00:00

abstract：:The random amplified polymorphic DNA (RAPD) technique was used to develop DNA probes for the identification of ectomycorrhizal fungi belonging to the genus Tuber. RAPD fingerprinting revealed a high degree of interspecific variability and a low degree of intraspecific variability. One band (approximately 1.5 kb), cons...

journal_title：FEMS microbiology letters

authors： Lanfranco L,Wyss P,Marzachí C,Bonfante P

更新日期：1993-12-15 00:00:00

abstract：:Both form I and II ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) genes were detected in Thiobacillus intermedius by heterologous hybridization using specific probes from Anacystis nidulans and Rhodobacter sphaeroides, respectively. However, only the previously reported form I enzyme could be demonstrated i...

journal_title：FEMS microbiology letters

authors： Stoner MT,Shively JM

更新日期：1993-03-01 00:00:00

abstract：:Correct identification of Campylobacter jejuni and Campylobacter coli isolates to the species or subspecies level is a cumbersome but nevertheless important task for a routine diagnostic laboratory. The widely used biochemical tests might be often misleading while more sophisticated phenotypic or genotypic methods are...

journal_title：FEMS microbiology letters

authors： Steinbrueckner B,Haerter G,Pelz K,Kist M

更新日期：1999-10-15 00:00:00

abstract：:The relationship of RNase P RNA from anammox bacteria 'Candidatus Brocadia anammoxidans' and 'Candidatus Kuenenia stuttgartiensis' with that from other Planctomycetes was investigated. Newly identified rnpB gene sequences were aligned against existing planctomycete RNase P RNA sequences and secondary structures deduce...

journal_title：FEMS microbiology letters

authors： Butler MK,Op den Camp HJ,Harhangi HR,Lafi FF,Strous M,Fuerst JA

更新日期：2007-03-01 00:00:00