Abstract：
:A protein kinase from Dictyostelium discoideum which phosphorylates the synthetic peptide, calmodulin-dependent protein kinase substrate (CDPKS, amino acid sequence: PLRRTLSVAA) and is stimulated by Ca2+/calmodulin is described. This is the first report of a protein kinase with these characteristics in D. discoideum. The enzyme was partially purified by Q-Sepharose chromatography. The protein kinase is very labile, and rapidly loses Ca2+/calmodulin-dependence upon standing at 4 degrees C, even in the presence of protease inhibitors, making further purification and characterisation difficult. In the active fractions, a 55 kDa polypeptide is labelled with [gamma-32P]ATP in vitro under conditions in which intramolecular rather than intermolecular reactions are favoured. The phosphorylation of this peptide is stimulated in the presence of Ca2+ and calmodulin but not Ca2+ alone. Ca2+/calmodulin-dependent stimulation is inhibited in the presence of the calmodulin antagonist, trifluoperazine (TFP). It is proposed that the 55 kDa polypeptide may represent the autophosphorylated form of the enzyme.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Dunbar AJ,Wheldrake JFdoi
10.1111/j.1574-6968.1994.tb06623.xsubject
Has Abstractpub_date
1994-01-01 00:00:00pages
113-8issue
1eissn
0378-1097issn
1574-6968journal_volume
115pub_type
杂志文章abstract：:Bacillus anthracis can be identified on the basis of the detection of virulence factor genes located on two plasmids, pXO1 and pXO2. Thus isolates lacking both pXO1 and pXO2 are indistinguishable from closely related B. cereus group bacteria. We developed a multiplex PCR assay for characterization of B. anthracis isol...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1996.tb08548.x
更新日期：1996-11-15 00:00:00
abstract：:This communication presents experimental evidence that intact fragile (osmotic sensitive) yeasts can be electrofused and give viable hybrids. The yield increases with one order of magnitude for electrofusion of intact fragile yeasts with protoplasts of non-fragile ones. The yield of viable hybrids, obtained by electro...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/0378-1097(89)90078-5
更新日期：1989-07-01 00:00:00
abstract：:Alcaligenes sp. strain PPH degrades phenanthrene via 1-hydroxy-2-naphthoic acid (1-H2NA), 1,2-dihydroxynaphthalene (1,2-DHN), salicylic acid and catechol. Enzyme activity versus growth profile and heat stability studies suggested the presence of two distinct hydroxylases, namely 1-hydroxy-2-naphthoic acid hydroxylase ...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2010.02079.x
更新日期：2010-10-01 00:00:00
abstract：:The accumulation of 2-keto-3-deoxy-6-phosphogluconate, the key intermediate of the Entner-Doudoroff pathway, has long been thought to inhibit growth of bacteria, but careful measurements of 2-keto-3-deoxy-6-phosphogluconate accumulation by growing cells and the correlation of intracellular 2-keto-3-deoxy-6-phosphogluc...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1998.tb12870.x
更新日期：1998-02-15 00:00:00
abstract：:The ADP-ribosylation factor (ARF) family of GTPases are highly conserved from yeast to human and regulate vesicle budding. Sec7 domain containing proteins stimulate the guanine nucleotide exchange on Arf proteins, while ARF-GTPase activating proteins stimulate the hydrolysis of GTP. Since vesicle trafficking is import...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1093/femsle/fnx240
更新日期：2017-12-29 00:00:00
abstract：:Biotype, genome, protein and plasmid profile diversity amongst 40 epidemiologically unrelated strains of Helicobacter pylori was studied. Strains were API Zym biotypes II, III and IV but most (87%) were biotype II. Four subsets of strains were defined on a combination of motility (56% positive) and cytotoxin productio...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/0378-1097(91)90086-p
更新日期：1991-04-15 00:00:00
abstract：:Brief exposure of Pseudomonas aeruginosa to a temperature of 10 degrees C or lower caused a significant leakage of the periplasmic beta-lactamase into the medium. The extent of leakage increased as the incubation temperature was lowered to 4 degrees C and reached a maximum at 0 degrees C. Cells grown in the presence o...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/0378-1097(91)90620-p
更新日期：1991-05-15 00:00:00
abstract：:The growth of Escherichia coli is inhibited by an antibiotic compound, azaserine (O-diazoacetyl-L-serine). Previous studies revealed the biochemical properties of azaserine, which involves inhibition of various enzymatic reactions as well as introduction of DNA breakage. However, genetically, nothing has been elucidat...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/j.femsle.2004.06.017
更新日期：2004-08-01 00:00:00
abstract：:A native collection of Bacillus thuringiensis strains was screened, once a reliable bioassay technique to assess the toxicity against the coffee berry borer (CBB) first-instar larvae was developed. A first round of bioassays with 170 strains indicated that the great majority of them showed no or very little insecticid...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/S0378-1097(03)00557-3
更新日期：2003-09-12 00:00:00
abstract：:Three strains of Streptococcus uberis isolated from dairy cows with mastitis were co-cultured with a bovine mammary epithelial cell line (MAC-T) in Dulbecco's modified Eagle's medium without fetal bovine serum. Protein profiles from culture supernatants and bacterial pellets among different treatments were compared by...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1998.tb13896.x
更新日期：1998-09-15 00:00:00
abstract：:The detection of ancient microbial DNA offers a new approach for the study of infectious diseases, their occurrence, frequency and host-pathogen interaction in historic times and populations. Moreover, data obtained from skeletal and mummified tissue may represent an important completion of contemporary phylogenetic a...
journal_title：FEMS microbiology letters
pub_type： 历史文章,杂志文章,评审
doi：10.1111/j.1574-6968.2002.tb11298.x
更新日期：2002-08-06 00:00:00
abstract：:Cytoplasmic precursors of the peptidoglycan biosynthetic pathway were purified from vancomycin-treated, glycopeptide-sensitive and -resistant strains of Enterococcus faecium. Resistance was due to production of a modified precursor, UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-lactate, where lactate was identified on the basi...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/0378-1097(92)90608-q
更新日期：1992-07-01 00:00:00
abstract：:Defects in the acd gene (which may be allelic to ubiH) result in the inactivation of the coenzyme A-linked acetaldehyde dehydrogenase activity of the multifunctional AdhE protein of Escherichia coli. This activity is restored by addition of ubiquinone-0 to cell extracts. However, the alcohol dehydrogenase activity of ...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2000.tb08872.x
更新日期：2000-01-01 00:00:00
abstract：:In the transition to the post-petroleum economy, there is a growing demand for novel enzymes with high process performances to replace traditional chemistry with a more 'green' approach. To date, microorganisms encompass the richest source of industrial biocatalysts, but the Earth-living microbiota remains largely unt...
journal_title：FEMS microbiology letters
pub_type： 杂志文章,评审
doi：10.1093/femsle/fnx211
更新日期：2017-11-15 00:00:00
abstract：:The production of exopolysaccharides (EPSs) by a mucoid clinical isolate of Burkholderia cepacia involved in infections in cystic fibrosis patients, was studied. Depending on the growth conditions, this strain was able to produce two different EPS, namely PS-I and PS-II, either alone or together. PS-I is composed of e...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2001.tb10792.x
更新日期：2001-08-07 00:00:00
abstract：:Molecular studies of the rumen bacterium Ruminococcus flavefaciens are constrained by the lack of stable gene transfer systems. We report here on the characterization of RflFII, a restriction endonuclease isolated from R. flavefaciens FD-1. The enzyme is an isoschizomer of ScaI, and cleavage of the DNA is blunt-ended,...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1994.tb07162.x
更新日期：1994-09-15 00:00:00
abstract：:Despite the large number of techniques available for the transformation of bacteria, several species are still resistant to the introduction of foreign DNA. Oenococcus oeni are among the organisms that are particularly refractory to transformation. However, conjugal experiments from Lactococcus lactis to O. oeni with ...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/j.femsle.2004.05.029
更新日期：2004-07-01 00:00:00
abstract：:Three heterocytous cyanobacterial strains were isolated from different habitats of Central India, and initial morphological studies indicated them to be members of the genus Nostoc or other closely related genera. Subsequent studies using morphological, ecological, molecular and phylogenetic methods indicated the thre...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1093/femsle/fny272
更新日期：2018-12-01 00:00:00
abstract：:We have observed significant cell lysis upon temperature up-shift of recombinant Escherichia coli cultures harboring CI857-repressed lambda-based expression vectors. This event, that becomes evident about 30-40 min after the heat shock, takes place when using the lambda promoter system in Ind- lysogenic strains, but n...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1999.tb13750.x
更新日期：1999-08-15 00:00:00
abstract：:The genes for fructosyltransferase (ftf) and glucan-binding protein (gbp) of Streptococcus mutans strain Ingbritt have been cloned in E. coli using bacteriophage and plasmid vectors. A single ftf gene appears to be responsible for the appearance of several FTF of different electrophoretic mobilities. Despite the fact ...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/0378-1097(89)90432-1
更新日期：1989-06-01 00:00:00
abstract：:Six clinical isolates of Enterobacter cloacae isolated in a Portuguese hospital, between April 1999 and November 2000, demonstrated resistance to almost all broad-spectrum cephalosporins, except to cefepime. These isolates were susceptible to quinolones and to aminoglycosides. Isoelectric focusing demonstrated product...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/S0378-1097(03)00891-7
更新日期：2004-01-30 00:00:00
abstract：:Bradyrhizobium japonicum has two types of flagella. One has thin filaments consisting of the 33-kDa flagellins FliCI and FliCII (FliCI-II) and the other has thick filaments consisting of the 65-kDa flagellins FliC1, FliC2, FliC3, and FliC4 (FliC1-4). To investigate the roles of each flagellum in competition for nodula...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2011.02280.x
更新日期：2011-06-01 00:00:00
abstract：:The genes encoding the desulforubidin type of dissimilatory sulfite reductase (Dsr) from the sulfate-reducing bacteria Desulfobacter vibrioformis and Desulfobulbus rhabdoformis were cloned and sequenced. Similar to the genes for dissimilatory sulfite reductase from the genera Archaeoglobus, Desulfovibrio and Desulfoto...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2000.tb09079.x
更新日期：2000-05-01 00:00:00
abstract：:Naturally occurring antimicrobial cationic polypeptides play a major role in innate and adaptive immunity. These polypeptides are found to be either linear and unstructured or structured through disulfide bonds. Among the structured antimicrobial polypeptides, defensins comprise a family of cysteine-rich cationic poly...
journal_title：FEMS microbiology letters
pub_type： 杂志文章,评审
doi：10.1111/j.1574-6968.2002.tb10979.x
更新日期：2002-01-02 00:00:00
abstract：:The wine yeast Saccharomyces cerevisiae is central in the production of aroma compounds during fermentation. Some of the most important yeast-derived aroma compounds produced are esters. The esters ethyl acetate and isoamyl acetate are formed from alcohols and acetyl-CoA in a reaction catalysed by alcohol acetyltransf...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2006.00548.x
更新日期：2007-02-01 00:00:00
abstract：:The time course production of xylanolytic enzymes by the rumen anaerobic fungus Neocallimastix frontalis was studied during growth on different carbon sources and revealed using isoelectric focusing and immunoblotting. A constant low level of endoxylanase expression was observed in glucose medium. A high level of xyla...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1998.tb13066.x
更新日期：1998-07-01 00:00:00
abstract：:Occurrence of tetracycline resistance genes encoding ribosomal protection proteins was examined in 151 tetracycline-resistant bacterial isolates from fish and seawater at coastal aquaculture sites in Japan and Korea. The tet(M) gene was detected in 34 Japanese and Korean isolates, which included Vibrio sp., Lactococcu...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1016/j.femsle.2004.06.026
更新日期：2004-08-01 00:00:00
abstract：:Fire blight, caused by plant pathogenic bacterium Erwinia amylovora, is one of the most important diseases of Rosaceae plants. Due to the lack of effective control measures, fire blight infections pose a recurrent threat on agricultural production worldwide. Recently, bacterial viruses, or bacteriophages, have been pr...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1093/femsle/fnaa070
更新日期：2020-05-01 00:00:00
abstract：:The structure and distribution of a Mycobacterium bovis BCG insertion element of the IS21 family were investigated. Several IS21-like elements found in mycobacterial genomes were separated in four types, following their nucleic acid similarities. The M. bovis BCG IS21 element is highly similar to IS1533 (class I), 70%...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.1999.tb08679.x
更新日期：1999-09-15 00:00:00
abstract：:The polymerase chain reaction (PCR)-based procedures of randomly amplified polymorphic DNA (RAPD) and repetitive element (RE)-based PCR were used to amplify total DNA prepared from each of 62 clinical Serratia marcescens isolates. Three different random primers, designated 1060, 1254 and 1283, were used individually i...
journal_title：FEMS microbiology letters
pub_type： 杂志文章
doi：10.1111/j.1574-6968.2001.tb09440.x
更新日期：2001-01-01 00:00:00
